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The map label for this gene is waaA [H]

Identifier: 119773242

GI number: 119773242

Start: 113051

End: 114313

Strand: Direct

Name: waaA [H]

Synonym: Sama_0100

Alternate gene names: 119773242

Gene position: 113051-114313 (Clockwise)

Preceding gene: 119773236

Following gene: 119773244

Centisome position: 2.63

GC content: 57.32

Gene sequence:

>1263_bases
ATGGCTCGATTGCTCTATTCAGTTCTCTTATACCTGCTGTCGCCACTCTTGGTACTTTATCTGGCCCAGCGTGCCATAAA
GAGCCCGGATTATCGTGGTCGCTGGGGCGAGCGGTTTGGCCTTAAGGCGCTGATGCCTACCGACATACTGATCCATTCTG
TCTCCATGGGCGAGACGCTCGCAGCCATACCACTCATTCGCGCTATCATGGCGGCCAGGCCAAATCTGCGGATCACGGTT
ACCACGTCAAGCCCCACGGGCTCGGCAGAAGTTATCAAGGCCTTTGGCGACCGGGTACAACATTGTTATTTACCCTTTGA
CCTGGGGTTTGCCGTTAACCGCTTTTTGAGCCAATTGGGCTGCGCCGAGCTTATCATCATGGAAACCGAGCTGTGGCCAA
ACCTCATTGCCGGGGCCAGGGCTCGGGGCATCAAGGTATCTTTGCTCAATGCCCGTTTGTCAGAAAAATCCGCCGCCAGT
TATCAAAAGTTCGCATCCCTCACTGTGCCCATGCTCAGGTCGCTGGACCAGATTGCGGTGCAAACGACCAAAGAAGCAGA
ACGTTTTGTCGCACTTGGCGTGGATGCCTCGCGGGTACATGTGTGTGGTAGTTTGAAGTTTGATATCCAGATCCCCCCAT
CCCGCCGTGAATCGGCGCGGCAGTTGCGGCAGAGTTGGGGACGGGACGACCACTTGATATGGGTGGCGGGCAGCGTACAT
CCCGGTGAATTCGATACCATGCTCAGCGCCCATCACCAACTACTTGAGCAGTTTCCAGACGCGCTGCTGATTATGGTGCC
AAGGCATCCTGAGCAGTTTGCCAACGCTGCACGGGCCATACGTGAGAGAGGCTTACAGCTGGCCGTTCGCAGTATGGGAG
AGGCGCCGACAGTGGGCACGCAGGTGCTCCTTGGCGACACCATGGGCGAGCTCTTGACCTGGTACGGCGCTGCCGATCTG
GCCTTTGTGGGCGGTACACTGGTGGTCAATGGCGGCCACAATCCGCTGGAGCCTGCGGCCATGGGGTTACCGGTTGCCAT
GGGACCCAACCATTGGGATTTTAAAGAAATTACCGAACTTCTGCAGCAGGCGGGTAATCTCACCCTGTGTGCTACCCCGG
ACGCCTTTAACGACTGGTTGTTGGCGACTGCACAGGATTCGGTTGGCCGCCTGACCGCCGGTGAGGCTGGGTTAAGAGTC
GTGGAGAGCAATCGTGGGGCGCTGAGTCGTCAACTGGCGGTATTGGGTTACGCAGCAGCGTAA

Upstream 100 bases:

>100_bases
GCCGCTTCCTGGCTAATCGCTCTGTGCTTATTCCTGCTTCCCGGCTTATGTTAACATTGCCGTAATTCATCAGGCAAAAC
TCATACATCTCTTGGTACTT

Downstream 100 bases:

>100_bases
CCCTCCAGTAGCCATTGCCAGCAGGACTCATCGAAGGCAAGCGAAGGAAGCTTGCCTTTTTCTTTATCAAAAGAGCGTCT
CAGTCTGGACAGGTTGGCTT

Product: 3-deoxy-D-manno-octulosonic-acid (KDO) transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MARLLYSVLLYLLSPLLVLYLAQRAIKSPDYRGRWGERFGLKALMPTDILIHSVSMGETLAAIPLIRAIMAARPNLRITV
TTSSPTGSAEVIKAFGDRVQHCYLPFDLGFAVNRFLSQLGCAELIIMETELWPNLIAGARARGIKVSLLNARLSEKSAAS
YQKFASLTVPMLRSLDQIAVQTTKEAERFVALGVDASRVHVCGSLKFDIQIPPSRRESARQLRQSWGRDDHLIWVAGSVH
PGEFDTMLSAHHQLLEQFPDALLIMVPRHPEQFANAARAIRERGLQLAVRSMGEAPTVGTQVLLGDTMGELLTWYGAADL
AFVGGTLVVNGGHNPLEPAAMGLPVAMGPNHWDFKEITELLQQAGNLTLCATPDAFNDWLLATAQDSVGRLTAGEAGLRV
VESNRGALSRQLAVLGYAAA

Sequences:

>Translated_420_residues
MARLLYSVLLYLLSPLLVLYLAQRAIKSPDYRGRWGERFGLKALMPTDILIHSVSMGETLAAIPLIRAIMAARPNLRITV
TTSSPTGSAEVIKAFGDRVQHCYLPFDLGFAVNRFLSQLGCAELIIMETELWPNLIAGARARGIKVSLLNARLSEKSAAS
YQKFASLTVPMLRSLDQIAVQTTKEAERFVALGVDASRVHVCGSLKFDIQIPPSRRESARQLRQSWGRDDHLIWVAGSVH
PGEFDTMLSAHHQLLEQFPDALLIMVPRHPEQFANAARAIRERGLQLAVRSMGEAPTVGTQVLLGDTMGELLTWYGAADL
AFVGGTLVVNGGHNPLEPAAMGLPVAMGPNHWDFKEITELLQQAGNLTLCATPDAFNDWLLATAQDSVGRLTAGEAGLRV
VESNRGALSRQLAVLGYAAA
>Mature_419_residues
ARLLYSVLLYLLSPLLVLYLAQRAIKSPDYRGRWGERFGLKALMPTDILIHSVSMGETLAAIPLIRAIMAARPNLRITVT
TSSPTGSAEVIKAFGDRVQHCYLPFDLGFAVNRFLSQLGCAELIIMETELWPNLIAGARARGIKVSLLNARLSEKSAASY
QKFASLTVPMLRSLDQIAVQTTKEAERFVALGVDASRVHVCGSLKFDIQIPPSRRESARQLRQSWGRDDHLIWVAGSVHP
GEFDTMLSAHHQLLEQFPDALLIMVPRHPEQFANAARAIRERGLQLAVRSMGEAPTVGTQVLLGDTMGELLTWYGAADLA
FVGGTLVVNGGHNPLEPAAMGLPVAMGPNHWDFKEITELLQQAGNLTLCATPDAFNDWLLATAQDSVGRLTAGEAGLRVV
ESNRGALSRQLAVLGYAAA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=417, Percent_Identity=48.6810551558753, Blast_Score=383, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 45723; Mature: 45591

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLLYSVLLYLLSPLLVLYLAQRAIKSPDYRGRWGERFGLKALMPTDILIHSVSMGETL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCHHH
AAIPLIRAIMAARPNLRITVTTSSPTGSAEVIKAFGDRVQHCYLPFDLGFAVNRFLSQLG
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCC
CAELIIMETELWPNLIAGARARGIKVSLLNARLSEKSAASYQKFASLTVPMLRSLDQIAV
CCEEEEEEHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTTKEAERFVALGVDASRVHVCGSLKFDIQIPPSRRESARQLRQSWGRDDHLIWVAGSVH
HHHHHHHHEEEECCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCC
PGEFDTMLSAHHQLLEQFPDALLIMVPRHPEQFANAARAIRERGLQLAVRSMGEAPTVGT
CCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QVLLGDTMGELLTWYGAADLAFVGGTLVVNGGHNPLEPAAMGLPVAMGPNHWDFKEITEL
EEEEHHHHHHHHHHHCCCHHEEECCEEEECCCCCCCCHHHCCCCEEECCCCCCHHHHHHH
LQQAGNLTLCATPDAFNDWLLATAQDSVGRLTAGEAGLRVVESNRGALSRQLAVLGYAAA
HHHCCCEEEEECCCCCCCCEEEECCCCCCCEECCCCCCEEEECCCCHHHHHHHHHEECCC
>Mature Secondary Structure 
ARLLYSVLLYLLSPLLVLYLAQRAIKSPDYRGRWGERFGLKALMPTDILIHSVSMGETL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCHHH
AAIPLIRAIMAARPNLRITVTTSSPTGSAEVIKAFGDRVQHCYLPFDLGFAVNRFLSQLG
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHCC
CAELIIMETELWPNLIAGARARGIKVSLLNARLSEKSAASYQKFASLTVPMLRSLDQIAV
CCEEEEEEHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTTKEAERFVALGVDASRVHVCGSLKFDIQIPPSRRESARQLRQSWGRDDHLIWVAGSVH
HHHHHHHHEEEECCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCC
PGEFDTMLSAHHQLLEQFPDALLIMVPRHPEQFANAARAIRERGLQLAVRSMGEAPTVGT
CCHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QVLLGDTMGELLTWYGAADLAFVGGTLVVNGGHNPLEPAAMGLPVAMGPNHWDFKEITEL
EEEEHHHHHHHHHHHCCCHHEEECCEEEECCCCCCCCHHHCCCCEEECCCCCCHHHHHHH
LQQAGNLTLCATPDAFNDWLLATAQDSVGRLTAGEAGLRVVESNRGALSRQLAVLGYAAA
HHHCCCEEEEECCCCCCCCEEEECCCCCCCEECCCCCCEEEECCCCHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]