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The map label for this gene is def [H]

Identifier: 119773185

GI number: 119773185

Start: 47066

End: 47569

Strand: Reverse

Name: def [H]

Synonym: Sama_0043

Alternate gene names: 119773185

Gene position: 47569-47066 (Counterclockwise)

Preceding gene: 119773189

Following gene: 119773184

Centisome position: 1.1

GC content: 49.21

Gene sequence:

>504_bases
ATGCCTCTATTAAAAGTTTTACGTTTTCCAGACGAAAGATTAAGGACAGTTGCCAAGCCGGTGACGGAATTTACACCCGC
TCTGCAATCTCAAATCGACGACATGTTTGAAACCATGTACGAAGAAAATGGCATTGGCCTGGCCGCAACTCAGGTCGATT
TCCATCAACGCCTGATTGTGATGGATCTGCAAGATGAAGTTGAGCGACCCAAGGTGTTCATTAACCCTGAGATTGTGGCC
AAGAGCGGTGACTTCTGTAACGAGGAAGGCTGCCTGTCGGTGCCTGGCGTCTACGCTAAGGTTGATCGCGCCGAGTTAAT
TACAGTTAAGGCGCTTGACCGTAATGGCAATGAATTTACGGTAGAGGCCGACGGCCTGTTCGCCATTTGCCTGCAACATG
AGATGGACCATCTCGTCGGCAAGCTCTTTGTTGACTATCTTTCTCCCCTGAAGCGTCAGCGTATTCGCCAAAAGCTGGAA
AAAGCCGCCCGTAACGACAAATAA

Upstream 100 bases:

>100_bases
TTTACTGTTATTGATTGTCGCTACGGATTAAAATTGACCCAATAGCAAAGTCAGTATAACCAACTGGCACGGATTTGACA
GACTTGTTAAGAGTTTAAGT

Downstream 100 bases:

>100_bases
GATTCAGGAATCGTTTTGAAGCCACTGAAAATCATCTTTGCTGGCACGCCGGATTTTGCTGCCCGCCACCTGCAGGCCCT
GCTGACATCTGAGCATGAAG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MPLLKVLRFPDERLRTVAKPVTEFTPALQSQIDDMFETMYEENGIGLAATQVDFHQRLIVMDLQDEVERPKVFINPEIVA
KSGDFCNEEGCLSVPGVYAKVDRAELITVKALDRNGNEFTVEADGLFAICLQHEMDHLVGKLFVDYLSPLKRQRIRQKLE
KAARNDK

Sequences:

>Translated_167_residues
MPLLKVLRFPDERLRTVAKPVTEFTPALQSQIDDMFETMYEENGIGLAATQVDFHQRLIVMDLQDEVERPKVFINPEIVA
KSGDFCNEEGCLSVPGVYAKVDRAELITVKALDRNGNEFTVEADGLFAICLQHEMDHLVGKLFVDYLSPLKRQRIRQKLE
KAARNDK
>Mature_166_residues
PLLKVLRFPDERLRTVAKPVTEFTPALQSQIDDMFETMYEENGIGLAATQVDFHQRLIVMDLQDEVERPKVFINPEIVAK
SGDFCNEEGCLSVPGVYAKVDRAELITVKALDRNGNEFTVEADGLFAICLQHEMDHLVGKLFVDYLSPLKRQRIRQKLEK
AARNDK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=166, Percent_Identity=31.3253012048193, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=63.4146341463415, Blast_Score=214, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24645726, Length=140, Percent_Identity=30.7142857142857, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645728, Length=133, Percent_Identity=31.5789473684211, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19051; Mature: 18920

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLKVLRFPDERLRTVAKPVTEFTPALQSQIDDMFETMYEENGIGLAATQVDFHQRLIV
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHEEE
MDLQDEVERPKVFINPEIVAKSGDFCNEEGCLSVPGVYAKVDRAELITVKALDRNGNEFT
EECHHHHCCCEEEECCEEEECCCCCCCCCCCCCCCCHHHCCCCHHEEEEEEECCCCCEEE
VEADGLFAICLQHEMDHLVGKLFVDYLSPLKRQRIRQKLEKAARNDK
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLLKVLRFPDERLRTVAKPVTEFTPALQSQIDDMFETMYEENGIGLAATQVDFHQRLIV
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHEEE
MDLQDEVERPKVFINPEIVAKSGDFCNEEGCLSVPGVYAKVDRAELITVKALDRNGNEFT
EECHHHHCCCEEEECCEEEECCCCCCCCCCCCCCCCHHHCCCCHHEEEEEEECCCCCEEE
VEADGLFAICLQHEMDHLVGKLFVDYLSPLKRQRIRQKLEKAARNDK
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]