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The map label for this gene is yqhT [H]

Identifier: 119718693

GI number: 119718693

Start: 4729658

End: 4730905

Strand: Reverse

Name: yqhT [H]

Synonym: Noca_4474

Alternate gene names: 119718693

Gene position: 4730905-4729658 (Counterclockwise)

Preceding gene: 119718694

Following gene: 119718692

Centisome position: 94.89

GC content: 69.55

Gene sequence:

>1248_bases
ATGAGCGCCCCGAACCGACCGCTCCCGGCGCCCGGGCACATGGCGGTCGACTACGAGGAGCGGGTCGACTTCGACCGGCT
GCGCCACTACCGGCTCGGCCGGGCCCAGGCCGCGCTGGAGGGCAGCGAGTGCGGGGCCTTCCTGCTCTTCGACTTCTACA
ACATCCGCTACACCACGCACACCTGGATCGGCGGGGCGCTCGGCGACAAGATGATCCGCTACGCGCTGGTCGCGCGCGGC
AAAGAGCCGGTGCTCTGGGACTTCGGGTCCGCGGTCAAGCACCACAAGATCTACTCCCAGTGGGTGCCCGAGGAGAACTA
CCGGGCCGGGTTCCTCGGCTTCCGCGGCGCGGTCGCCCCGAGCGTCGGGCTGATGGAGACCGCGGTCGCGGAGATCAAGT
CGCTGCTGGTCGAGGCCGGCGTCGCCGACCTCCCGGTCGGCGTGGACATCGTGGAGCCGCCGTTCCTCTTCGAGATGCAG
CGTCAGGGCCTGACCGTCGTCGACGCCCAGCAGCTGATGCTCGACGCACGCTGCATCAAGTCCCACGACGAGATCGTGCT
GCTCAACCAGGCCGCCGCGATGGTCGACGGCGTCTACCAGGACATCGTCGAGGCGCTCAAGCCCGGCGTGCGCGAGAACG
AGATCGTCGCGCTCGCCAACAAGCGGCTCTACGAGATGGGCTCGGACCAGGTCGAGGCCGTCAACGCGATCTCCGGCGAA
CGCTGCAACCCGCACCCGCACAACTTCACCGACCGGCTGATCCGCCCCGGCGACCAGGCGTTCTTCGACATCATCCACTC
CTTCAACGGCTACCGGACCTGCTACTACCGCACGTTCTCGGTCGGCAGCGCGACCCCGGCCCAGCGCGACGCCTACACCC
AGGCGCGGGAGTGGATGGACCGCGGCATCGACGGCATCCGCGCCGGCGTCGGCACCGACGAGGTGGCCGCGCTGCTGCCC
GAGGCCGAGGAGTTCGGCTTCGGCTCCGAGATGGAGGCCTTCGGCCTCCAGTTCGCCCACGGGCTCGGCCTCGGCCTGCA
CGAGCGGCCGATCATCTCCCGGCTCAACTCGATGAAGGAGCCGGTCGAGCTCCAGGTCGGCATGGTCTTCGCGCTGGAGA
CCTACTGCCCGGCCTCCGACGGCGTCTCCGCGGCCCGGATCGAGGAGGAGATCGTGATCACCGAGGACGGCCCCCGGGTG
CTCACCCTCTTCCCGGCGCAGGACCTGGTCGTCGCCAACCCCTACTAG

Upstream 100 bases:

>100_bases
ACTTCGCGATCCTGCTCGACCTCCAGGCGCACAACTCCGGCATCACCCTCAAGGCCGAGAACGTCCAGGTCGACGACGGG
CTCGGCGGAGAGCTGGGCTG

Downstream 100 bases:

>100_bases
CAGCACCCGAAAGGCAACCCCCATGGCTGACTTCACCCTCGCGCAGATCCCCGACCTGCCGCTCGACCTCTGCGTCGGCG
GGAAGGAGGTCCCGGCGTCC

Product: peptidase M24

Products: N-terminal aminoacid; peptide preferentially methionine [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSAPNRPLPAPGHMAVDYEERVDFDRLRHYRLGRAQAALEGSECGAFLLFDFYNIRYTTHTWIGGALGDKMIRYALVARG
KEPVLWDFGSAVKHHKIYSQWVPEENYRAGFLGFRGAVAPSVGLMETAVAEIKSLLVEAGVADLPVGVDIVEPPFLFEMQ
RQGLTVVDAQQLMLDARCIKSHDEIVLLNQAAAMVDGVYQDIVEALKPGVRENEIVALANKRLYEMGSDQVEAVNAISGE
RCNPHPHNFTDRLIRPGDQAFFDIIHSFNGYRTCYYRTFSVGSATPAQRDAYTQAREWMDRGIDGIRAGVGTDEVAALLP
EAEEFGFGSEMEAFGLQFAHGLGLGLHERPIISRLNSMKEPVELQVGMVFALETYCPASDGVSAARIEEEIVITEDGPRV
LTLFPAQDLVVANPY

Sequences:

>Translated_415_residues
MSAPNRPLPAPGHMAVDYEERVDFDRLRHYRLGRAQAALEGSECGAFLLFDFYNIRYTTHTWIGGALGDKMIRYALVARG
KEPVLWDFGSAVKHHKIYSQWVPEENYRAGFLGFRGAVAPSVGLMETAVAEIKSLLVEAGVADLPVGVDIVEPPFLFEMQ
RQGLTVVDAQQLMLDARCIKSHDEIVLLNQAAAMVDGVYQDIVEALKPGVRENEIVALANKRLYEMGSDQVEAVNAISGE
RCNPHPHNFTDRLIRPGDQAFFDIIHSFNGYRTCYYRTFSVGSATPAQRDAYTQAREWMDRGIDGIRAGVGTDEVAALLP
EAEEFGFGSEMEAFGLQFAHGLGLGLHERPIISRLNSMKEPVELQVGMVFALETYCPASDGVSAARIEEEIVITEDGPRV
LTLFPAQDLVVANPY
>Mature_414_residues
SAPNRPLPAPGHMAVDYEERVDFDRLRHYRLGRAQAALEGSECGAFLLFDFYNIRYTTHTWIGGALGDKMIRYALVARGK
EPVLWDFGSAVKHHKIYSQWVPEENYRAGFLGFRGAVAPSVGLMETAVAEIKSLLVEAGVADLPVGVDIVEPPFLFEMQR
QGLTVVDAQQLMLDARCIKSHDEIVLLNQAAAMVDGVYQDIVEALKPGVRENEIVALANKRLYEMGSDQVEAVNAISGER
CNPHPHNFTDRLIRPGDQAFFDIIHSFNGYRTCYYRTFSVGSATPAQRDAYTQAREWMDRGIDGIRAGVGTDEVAALLPE
AEEFGFGSEMEAFGLQFAHGLGLGLHERPIISRLNSMKEPVELQVGMVFALETYCPASDGVSAARIEEEIVITEDGPRVL
TLFPAQDLVVANPY

Specific function: Removes The Amino-Terminal Methionine From Nascent Proteins. [C]

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family [H]

Homologues:

None

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR001131 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.11.18 [C]

Molecular weight: Translated: 45928; Mature: 45797

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPNRPLPAPGHMAVDYEERVDFDRLRHYRLGRAQAALEGSECGAFLLFDFYNIRYTTH
CCCCCCCCCCCCCEEECHHHHCCHHHHHHHHCCHHHHHCCCCCCCEEEEEEEEEEEEEEE
TWIGGALGDKMIRYALVARGKEPVLWDFGSAVKHHKIYSQWVPEENYRAGFLGFRGAVAP
HHCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCC
SVGLMETAVAEIKSLLVEAGVADLPVGVDIVEPPFLFEMQRQGLTVVDAQQLMLDARCIK
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHCCCEEEEHHHHHHHHHHHC
SHDEIVLLNQAAAMVDGVYQDIVEALKPGVRENEIVALANKRLYEMGSDQVEAVNAISGE
CCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHCCCCHHHHHHHCCCC
RCNPHPHNFTDRLIRPGDQAFFDIIHSFNGYRTCYYRTFSVGSATPAQRDAYTQAREWMD
CCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHH
RGIDGIRAGVGTDEVAALLPEAEEFGFGSEMEAFGLQFAHGLGLGLHERPIISRLNSMKE
HCHHHHHCCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
PVELQVGMVFALETYCPASDGVSAARIEEEIVITEDGPRVLTLFPAQDLVVANPY
CCEEEEEEEEEEEECCCCCCCCCHHEECCEEEEECCCCEEEEEECCCCEEEECCC
>Mature Secondary Structure 
SAPNRPLPAPGHMAVDYEERVDFDRLRHYRLGRAQAALEGSECGAFLLFDFYNIRYTTH
CCCCCCCCCCCCEEECHHHHCCHHHHHHHHCCHHHHHCCCCCCCEEEEEEEEEEEEEEE
TWIGGALGDKMIRYALVARGKEPVLWDFGSAVKHHKIYSQWVPEENYRAGFLGFRGAVAP
HHCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCC
SVGLMETAVAEIKSLLVEAGVADLPVGVDIVEPPFLFEMQRQGLTVVDAQQLMLDARCIK
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHCCCEEEEHHHHHHHHHHHC
SHDEIVLLNQAAAMVDGVYQDIVEALKPGVRENEIVALANKRLYEMGSDQVEAVNAISGE
CCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHCCCCHHHHHHHCCCC
RCNPHPHNFTDRLIRPGDQAFFDIIHSFNGYRTCYYRTFSVGSATPAQRDAYTQAREWMD
CCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHH
RGIDGIRAGVGTDEVAALLPEAEEFGFGSEMEAFGLQFAHGLGLGLHERPIISRLNSMKE
HCHHHHHCCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
PVELQVGMVFALETYCPASDGVSAARIEEEIVITEDGPRVLTLFPAQDLVVANPY
CCEEEEEEEEEEEECCCCCCCCCHHEECCEEEEECCCCEEEEEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Peptide; H2O [C]

Specific reaction: peptide + H2O = N-terminal aminoacid + peptide [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: EDTA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]