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The map label for this gene is hycG [H]

Identifier: 119718689

GI number: 119718689

Start: 4725228

End: 4725716

Strand: Reverse

Name: hycG [H]

Synonym: Noca_4470

Alternate gene names: 119718689

Gene position: 4725716-4725228 (Counterclockwise)

Preceding gene: 119718690

Following gene: 119718688

Centisome position: 94.78

GC content: 76.28

Gene sequence:

>489_bases
ATGAGTCTCGCCCGACTGGCCGGGCTGATCCGCCGGACCGGGCGGATCGCCGAGCCGGCGCCGGCGCTCCCCGACGGCCA
CCCCGGCCGTCCGACCGAGCTGGCCGGCAGCGTGCAGATCCGGCACGTCGACGCCGGGTCGTGCAACGGCTGCGAGATCG
AGGTCGGCGGGGCCTTCGGCCCGGTCTACGACGCGGAGCGGTACGGCGCACGCCTGGTCGCCTCGCCACGACATGCCGAC
GTCGTCCTGGTCACCGGCGTGGTGACGCACAACATGCTCGAGCCGCTGCGCCGTACCGTCGAGGCCACGCCGCGGCCGCG
GCTCGTGCTGGCCGTGGGCGACTGCGCCCGCAACTGCGGCGCCTTCAAGGGCGGGTACGGCGTCGCCGGCGCCGTGCACG
ACGCGGTCGACGTCGACCTCGAGGTCCCGGGCTGCCCGCCCGAGCCGGCCGCCATCGTCGACGCGCTGCGCCGGGTGACC
GGACGGTGA

Upstream 100 bases:

>100_bases
TCCCGGAGACCAGCGACCTGCTCCTGGCCGCCCGGCGCATCTTGCGGCTGCTCCCGGTGCAGGTCGAGCTGCGGGCGGAG
CTCGAGAGCGGCGAACCCCG

Downstream 100 bases:

>100_bases
GCCTGCGGATGGGTCGACGGTGACCGGTGTCGAGCTGGGCATGACCGCGCAGGTCGTGCTCGCGGCCGCAGCCGTCGCGG
CCGCGGTGCTGGCCCCGCCC

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSLARLAGLIRRTGRIAEPAPALPDGHPGRPTELAGSVQIRHVDAGSCNGCEIEVGGAFGPVYDAERYGARLVASPRHAD
VVLVTGVVTHNMLEPLRRTVEATPRPRLVLAVGDCARNCGAFKGGYGVAGAVHDAVDVDLEVPGCPPEPAAIVDALRRVT
GR

Sequences:

>Translated_162_residues
MSLARLAGLIRRTGRIAEPAPALPDGHPGRPTELAGSVQIRHVDAGSCNGCEIEVGGAFGPVYDAERYGARLVASPRHAD
VVLVTGVVTHNMLEPLRRTVEATPRPRLVLAVGDCARNCGAFKGGYGVAGAVHDAVDVDLEVPGCPPEPAAIVDALRRVT
GR
>Mature_161_residues
SLARLAGLIRRTGRIAEPAPALPDGHPGRPTELAGSVQIRHVDAGSCNGCEIEVGGAFGPVYDAERYGARLVASPRHADV
VLVTGVVTHNMLEPLRRTVEATPRPRLVLAVGDCARNCGAFKGGYGVAGAVHDAVDVDLEVPGCPPEPAAIVDALRRVTG
R

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=103, Percent_Identity=36.8932038834951, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=42.6229508196721, Blast_Score=113, Evalue=6e-27,
Organism=Escherichia coli, GI1788834, Length=117, Percent_Identity=44.4444444444444, Blast_Score=109, Evalue=7e-26,
Organism=Escherichia coli, GI1788624, Length=111, Percent_Identity=36.9369369369369, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17509685, Length=103, Percent_Identity=37.8640776699029, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18859983, Length=103, Percent_Identity=38.8349514563107, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24642371, Length=103, Percent_Identity=38.8349514563107, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24651058, Length=112, Percent_Identity=35.7142857142857, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 16858; Mature: 16726

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLARLAGLIRRTGRIAEPAPALPDGHPGRPTELAGSVQIRHVDAGSCNGCEIEVGGAFG
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCC
PVYDAERYGARLVASPRHADVVLVTGVVTHNMLEPLRRTVEATPRPRLVLAVGDCARNCG
CCCCHHHHCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCC
AFKGGYGVAGAVHDAVDVDLEVPGCPPEPAAIVDALRRVTGR
CCCCCCCCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLARLAGLIRRTGRIAEPAPALPDGHPGRPTELAGSVQIRHVDAGSCNGCEIEVGGAFG
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCC
PVYDAERYGARLVASPRHADVVLVTGVVTHNMLEPLRRTVEATPRPRLVLAVGDCARNCG
CCCCHHHHCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCC
AFKGGYGVAGAVHDAVDVDLEVPGCPPEPAAIVDALRRVTGR
CCCCCCCCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]