Click here to switch to the map view.

The map label for this gene is hyfB [C]

Identifier: 119718688

GI number: 119718688

Start: 4723214

End: 4725208

Strand: Reverse

Name: hyfB [C]

Synonym: Noca_4469

Alternate gene names: 119718688

Gene position: 4725208-4723214 (Counterclockwise)

Preceding gene: 119718689

Following gene: 119718687

Centisome position: 94.77

GC content: 74.39

Gene sequence:

>1995_bases
GTGACCGGTGTCGAGCTGGGCATGACCGCGCAGGTCGTGCTCGCGGCCGCAGCCGTCGCGGCCGCGGTGCTGGCCCCGCC
CCGGCTGCGCAACCTGGTGGCGGGGACACTTGCCGCCGCCGTCGGCGCGGCCGGCGTGGTGACCGGCGCCGCCGCGCTCA
CCGACCACTCCGGCTCGCTTGACCTGTCGACGGCCCTCCCCCTCACGGGCGTAGCCCTCGACCCCACGCCGCTCGGCGGC
TTCGTGATGCTGGTCGCGGGAGCGGTCGGCGCTGTGGCCGCCGTCTTCGCGATCGGCTACGCACACGGACCGGCCGCCTC
GCGCACCTCCTGGGCGGCGCTCCCCGCGTTCCTGGTCTCGATGCAGCTGGTGCCCGCGGCCGCAGATGCCGTCTCGTTCC
TGCTGATGTGGGAGCTGATGGCGCTCGCCTCGGCGGTGCTGGTCCTCACCGAGCACCGGGACCGAGAGGAAGTGCGGTCC
GCGGCACTCTGGTACGCCGCGATGACCCACCTGAGCTTCGGGCTGCTGCTGCTGGGCTTCACCGTGCTCGTGGAGGAGGG
CGGCGGCACCGGCTTCGACGCCATGAGCGCCGTCGACCCCACGTCCCTCCAGGCGTCGATCGCCTTCGTGCTGCTGATCG
CGGGTTTCGCGACCAAGGCGGGCGCGGTCCCGTTCCACGTCTGGTTGCCCCGGGCCCACCCGGCCGCGCCGAGCCACGTC
TCCGCGGTGATGAGCGCGGCGATGGTCAAGATGGGGGTGTACGGCGCGCTGCTGGTCGCGCTCCGCCTGCTGCCGGGCGG
CCCCACCTGGTGGGGGTTCGTCGTGGTGGCACTCGGCGCGGTGTCCGCGACGTACGGCATCCTCCAGGCCTCGGTCACCA
GCGACCTCAAGCGCCTGCTCGCGTACTCGACGACCGAGAACGTCGGGCTGATGTTCCTCGCGCTCGGGACGGCGATGCTC
CTGCGCTCCCACGATGTCTCGGCCCCGGCGGACGCGGCGCTCGTCGCCTGCCTGCTGCTGCTCACCAGTCACGCCGCCTT
CAAGACCGCACTGTTCCTCGGCGCGGGGGCCGTGCTGCACGCCACCGGTGAGCGGGACCTGGACCGAATGGGGGGTCTCG
CGGCCCGGATGCCGGTCACCAACGTGGCGTTCGCTCTCGGCGCTCTGGGCGCGGCGGCGCTCCCGCTGAGCGTGGGCTTC
GTGGCCGAGTGGACGCTGCTCCAGTCGCTGATCCACGGCGCCCGTCCGGACGATCGACTGGTCGCGGCGATGATGCCGAT
CTCGGTCGCCGTCGTCGCCCTCACGGCGGGGCTGGCGCTGCTCACGTTCGTCAAGGCCTACGGGATCGCCTTCCTGGCCC
GGCCCCGCTCGTCCGGTGCCGCCGAGGCCCGGGAGGCGACACCCGGCATGCGCACGGCGATGCTCGTGGCCGCGGTCGCC
GTCGTCGGCCTCGGCCTGGTTCCTGGCCCGCTCGCCGACCTCGCCGGGCGCGCCGCGGGTGTCGCCGATCCCGGGTCAGA
GGTGGCCGTCGACCTGCCCGGGATCGCGGGCGGCGTGCTCGATCCCGTCGCCGTGTCCGTCCTGGCGGTGCTGCTCGCGG
CGCCGGTCGGCGTGGTCGCGCTGGCCGCGGCACGGCGCCGCCGGCGGCGCCGGGTGGACCTCGCCTGGGGGTGCGGGGGC
GCGCGGGTCAGCCCGCGGATGCAGTACACCGCGACGTCGTACGCCGAACCCCTGGCGCGCGTGTTCGACGACGCCGTCCG
GCCGGAGCGCGACGTGGTGGTCACCCACTACAGCGAGTCCCGCTACCTGGTCGAGCGCGTGCAGTTCCGCCAGCGGCTCG
ACGAGGTCGTCGAGACCCGGCTCTACCAGCCCGTGCTGCGCGTCGGCGACCGTGTCGGGGCCGCGGCGCGCCGGCTGCAG
AACGGCAGCATCCATCGCTACCTGGGCTACTCCTTCGCCGCCCTGGTCGCCGTGCTCGTGGTGGTCGCGCTGTGA

Upstream 100 bases:

>100_bases
GACGTCGACCTCGAGGTCCCGGGCTGCCCGCCCGAGCCGGCCGCCATCGTCGACGCGCTGCGCCGGGTGACCGGACGGTG
AGCCTGCGGATGGGTCGACG

Downstream 100 bases:

>100_bases
GCGCCGGCGCGGTCGTGGTGACGGTGGTCCAGGCGGCGGTCCTGGTGGCGCTGTCCCCGCTGCTGGTGGGCGTGATGCGA
CAGGTGCGGGCCCGCCTCGA

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MTGVELGMTAQVVLAAAAVAAAVLAPPRLRNLVAGTLAAAVGAAGVVTGAAALTDHSGSLDLSTALPLTGVALDPTPLGG
FVMLVAGAVGAVAAVFAIGYAHGPAASRTSWAALPAFLVSMQLVPAAADAVSFLLMWELMALASAVLVLTEHRDREEVRS
AALWYAAMTHLSFGLLLLGFTVLVEEGGGTGFDAMSAVDPTSLQASIAFVLLIAGFATKAGAVPFHVWLPRAHPAAPSHV
SAVMSAAMVKMGVYGALLVALRLLPGGPTWWGFVVVALGAVSATYGILQASVTSDLKRLLAYSTTENVGLMFLALGTAML
LRSHDVSAPADAALVACLLLLTSHAAFKTALFLGAGAVLHATGERDLDRMGGLAARMPVTNVAFALGALGAAALPLSVGF
VAEWTLLQSLIHGARPDDRLVAAMMPISVAVVALTAGLALLTFVKAYGIAFLARPRSSGAAEAREATPGMRTAMLVAAVA
VVGLGLVPGPLADLAGRAAGVADPGSEVAVDLPGIAGGVLDPVAVSVLAVLLAAPVGVVALAAARRRRRRRVDLAWGCGG
ARVSPRMQYTATSYAEPLARVFDDAVRPERDVVVTHYSESRYLVERVQFRQRLDEVVETRLYQPVLRVGDRVGAAARRLQ
NGSIHRYLGYSFAALVAVLVVVAL

Sequences:

>Translated_664_residues
MTGVELGMTAQVVLAAAAVAAAVLAPPRLRNLVAGTLAAAVGAAGVVTGAAALTDHSGSLDLSTALPLTGVALDPTPLGG
FVMLVAGAVGAVAAVFAIGYAHGPAASRTSWAALPAFLVSMQLVPAAADAVSFLLMWELMALASAVLVLTEHRDREEVRS
AALWYAAMTHLSFGLLLLGFTVLVEEGGGTGFDAMSAVDPTSLQASIAFVLLIAGFATKAGAVPFHVWLPRAHPAAPSHV
SAVMSAAMVKMGVYGALLVALRLLPGGPTWWGFVVVALGAVSATYGILQASVTSDLKRLLAYSTTENVGLMFLALGTAML
LRSHDVSAPADAALVACLLLLTSHAAFKTALFLGAGAVLHATGERDLDRMGGLAARMPVTNVAFALGALGAAALPLSVGF
VAEWTLLQSLIHGARPDDRLVAAMMPISVAVVALTAGLALLTFVKAYGIAFLARPRSSGAAEAREATPGMRTAMLVAAVA
VVGLGLVPGPLADLAGRAAGVADPGSEVAVDLPGIAGGVLDPVAVSVLAVLLAAPVGVVALAAARRRRRRRVDLAWGCGG
ARVSPRMQYTATSYAEPLARVFDDAVRPERDVVVTHYSESRYLVERVQFRQRLDEVVETRLYQPVLRVGDRVGAAARRLQ
NGSIHRYLGYSFAALVAVLVVVAL
>Mature_663_residues
TGVELGMTAQVVLAAAAVAAAVLAPPRLRNLVAGTLAAAVGAAGVVTGAAALTDHSGSLDLSTALPLTGVALDPTPLGGF
VMLVAGAVGAVAAVFAIGYAHGPAASRTSWAALPAFLVSMQLVPAAADAVSFLLMWELMALASAVLVLTEHRDREEVRSA
ALWYAAMTHLSFGLLLLGFTVLVEEGGGTGFDAMSAVDPTSLQASIAFVLLIAGFATKAGAVPFHVWLPRAHPAAPSHVS
AVMSAAMVKMGVYGALLVALRLLPGGPTWWGFVVVALGAVSATYGILQASVTSDLKRLLAYSTTENVGLMFLALGTAMLL
RSHDVSAPADAALVACLLLLTSHAAFKTALFLGAGAVLHATGERDLDRMGGLAARMPVTNVAFALGALGAAALPLSVGFV
AEWTLLQSLIHGARPDDRLVAAMMPISVAVVALTAGLALLTFVKAYGIAFLARPRSSGAAEAREATPGMRTAMLVAAVAV
VGLGLVPGPLADLAGRAAGVADPGSEVAVDLPGIAGGVLDPVAVSVLAVLLAAPVGVVALAAARRRRRRRVDLAWGCGGA
RVSPRMQYTATSYAEPLARVFDDAVRPERDVVVTHYSESRYLVERVQFRQRLDEVVETRLYQPVLRVGDRVGAAARRLQN
GSIHRYLGYSFAALVAVLVVVAL

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=187, Percent_Identity=30.4812834224599, Blast_Score=91, Evalue=5e-18,
Organism=Escherichia coli, GI1788827, Length=556, Percent_Identity=35.9712230215827, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI2367154, Length=582, Percent_Identity=29.553264604811, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788831, Length=372, Percent_Identity=32.258064516129, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788614, Length=360, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788613, Length=393, Percent_Identity=26.2086513994911, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788829, Length=412, Percent_Identity=26.9417475728155, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693160, Length=351, Percent_Identity=25.6410256410256, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 68112; Mature: 67980

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVELGMTAQVVLAAAAVAAAVLAPPRLRNLVAGTLAAAVGAAGVVTGAAALTDHSGSL
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLSTALPLTGVALDPTPLGGFVMLVAGAVGAVAAVFAIGYAHGPAASRTSWAALPAFLVS
EECCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
MQLVPAAADAVSFLLMWELMALASAVLVLTEHRDREEVRSAALWYAAMTHLSFGLLLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TVLVEEGGGTGFDAMSAVDPTSLQASIAFVLLIAGFATKAGAVPFHVWLPRAHPAAPSHV
HHHEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
SAVMSAAMVKMGVYGALLVALRLLPGGPTWWGFVVVALGAVSATYGILQASVTSDLKRLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYSTTENVGLMFLALGTAMLLRSHDVSAPADAALVACLLLLTSHAAFKTALFLGAGAVLH
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ATGERDLDRMGGLAARMPVTNVAFALGALGAAALPLSVGFVAEWTLLQSLIHGARPDDRL
ECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VAAMMPISVAVVALTAGLALLTFVKAYGIAFLARPRSSGAAEAREATPGMRTAMLVAAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHCCCCHHHHHHHHHHH
VVGLGLVPGPLADLAGRAAGVADPGSEVAVDLPGIAGGVLDPVAVSVLAVLLAAPVGVVA
HHHCCCCCCCHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAAARRRRRRRVDLAWGCGGARVSPRMQYTATSYAEPLARVFDDAVRPERDVVVTHYSES
HHHHHHHHHHCCCEEECCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCEEEEEECCH
RYLVERVQFRQRLDEVVETRLYQPVLRVGDRVGAAARRLQNGSIHRYLGYSFAALVAVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VVAL
HHCC
>Mature Secondary Structure 
TGVELGMTAQVVLAAAAVAAAVLAPPRLRNLVAGTLAAAVGAAGVVTGAAALTDHSGSL
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLSTALPLTGVALDPTPLGGFVMLVAGAVGAVAAVFAIGYAHGPAASRTSWAALPAFLVS
EECCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
MQLVPAAADAVSFLLMWELMALASAVLVLTEHRDREEVRSAALWYAAMTHLSFGLLLLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TVLVEEGGGTGFDAMSAVDPTSLQASIAFVLLIAGFATKAGAVPFHVWLPRAHPAAPSHV
HHHEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
SAVMSAAMVKMGVYGALLVALRLLPGGPTWWGFVVVALGAVSATYGILQASVTSDLKRLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYSTTENVGLMFLALGTAMLLRSHDVSAPADAALVACLLLLTSHAAFKTALFLGAGAVLH
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ATGERDLDRMGGLAARMPVTNVAFALGALGAAALPLSVGFVAEWTLLQSLIHGARPDDRL
ECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VAAMMPISVAVVALTAGLALLTFVKAYGIAFLARPRSSGAAEAREATPGMRTAMLVAAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHCCCCHHHHHHHHHHH
VVGLGLVPGPLADLAGRAAGVADPGSEVAVDLPGIAGGVLDPVAVSVLAVLLAAPVGVVA
HHHCCCCCCCHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAAARRRRRRRVDLAWGCGGARVSPRMQYTATSYAEPLARVFDDAVRPERDVVVTHYSES
HHHHHHHHHHCCCEEECCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCCCEEEEEECCH
RYLVERVQFRQRLDEVVETRLYQPVLRVGDRVGAAARRLQNGSIHRYLGYSFAALVAVLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VVAL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]