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The map label for this gene is hycE [H]

Identifier: 119718684

GI number: 119718684

Start: 4718706

End: 4719881

Strand: Reverse

Name: hycE [H]

Synonym: Noca_4465

Alternate gene names: 119718684

Gene position: 4719881-4718706 (Counterclockwise)

Preceding gene: 119718685

Following gene: 119718683

Centisome position: 94.67

GC content: 73.3

Gene sequence:

>1176_bases
GTGCGGCACGGCCACTGGCCGCGCGGCTGGTACCCGATGCGCCGCGACGCCGGACCGCCGCCGCCGTTCGACGCCGACAT
CGGCGCCTTCCCGTTCCTCGAGGTCGAGGGCGACGGGGTCTACGAGATCCCTGTCGGGCCGGTGCACGCGGGCATGATCG
AGCCCGGTCACTTCCGGTTCTCGGTCATCGGCGAGACCATCCTGCGGCTGAAGGCCCGGCTGTGGTTCGTCCACCGCGGG
GTCGAGAAGCTCTTCGAGGGCCGCCGGCCCGAGGACGCCGTCGAGCTCGCCGAGCGGATCAGCGGGGACACGGCCGTCGG
CCACACGACCGCCTTCGCGACGGCCGTCGAGGACGCGCTCGGCATCGAGGTCCCCGAGCGGGACCGGCTGGGGCGCGCGC
TGCTGCTCGAGCTGGAGCGGATGCACAACCACGCGGCCGACCTCGGCGCGCTCGCCAACGACGTCGGCTACGGGATCGTC
AACACCCACGCGCTGCGGATCCGGGAGTCGCTGCTGCGCCTGAACAAGGCGACCACGGGCCACCGCCTGCTGCGAGGTGG
GGTCGGCATCGGTGGGACCCGGATCGACGCGCTCCCCGACCCGGCCGCCCTCGAGGCGGTCGCCGCCGAGGTCGCGGAGC
TCGCGGAGATCACGCTCGGCAACTCGACCGTGCGCGACCGGTTCACCGGGACCGCCGTCCTCGCCGCGGAGTCCGCGCGC
GACCTGGGCACCCTCGGCTATGTGGCCCGGGCCTCCGGCATCGACCGCGATGCGCGCCGCGACCACCCCTTCGTCGACCT
GGGCGACCGGTTCCACGTCGTCGTCGAGACCGGGGGCGACGTGCTAGCCCGGTACCGGGTCCGGGTGCGCGAGATCGCCG
TCTCCGCGGCGGTTGCCGGCGACCTCGTACGTCGGCTCAGCAGCGGCACCGGCGTCGACAGCCACACCTCGGCCCCGGGT
GGCGGGCACGGGCTCGGCCTGGTGGAAGGCTGGCGCGGCACCATCGCGCACAGGGTCGAGATCGACCCCTCCGGGCGGTT
GAGCCGGGTCAAGATCGTCGACCCGTCGTTCTTCAACTGGCCCGCGCTCCCGATCGCGCTCGCGGACACGATCGTCCCGG
ACTTCCCGCTCGCCAACAAGAGCTTCAACCAGTCGTACGCCGGCAACGACCTGTAG

Upstream 100 bases:

>100_bases
GTCGCTCGCCTACCTGGACTACGCCACCGGCCGGTTCGAGCGCGAGCTGCGCGACCTGTACGGCATCACGCCGTCCGGGC
ACCCGCTGCCCCGGCGCCTG

Downstream 100 bases:

>100_bases
GGGTCGTGCTGGGCAGGCGTCCCGATCCGGATGGCCAGCGACACCGAGTACGGCCTGGTCTCTCACCTCTACACCCGCGA
CCTGGCCCGCGGCCTGCGGA

Product: NADH-ubiquinone oxidoreductase, chain 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRHGHWPRGWYPMRRDAGPPPPFDADIGAFPFLEVEGDGVYEIPVGPVHAGMIEPGHFRFSVIGETILRLKARLWFVHRG
VEKLFEGRRPEDAVELAERISGDTAVGHTTAFATAVEDALGIEVPERDRLGRALLLELERMHNHAADLGALANDVGYGIV
NTHALRIRESLLRLNKATTGHRLLRGGVGIGGTRIDALPDPAALEAVAAEVAELAEITLGNSTVRDRFTGTAVLAAESAR
DLGTLGYVARASGIDRDARRDHPFVDLGDRFHVVVETGGDVLARYRVRVREIAVSAAVAGDLVRRLSSGTGVDSHTSAPG
GGHGLGLVEGWRGTIAHRVEIDPSGRLSRVKIVDPSFFNWPALPIALADTIVPDFPLANKSFNQSYAGNDL

Sequences:

>Translated_391_residues
MRHGHWPRGWYPMRRDAGPPPPFDADIGAFPFLEVEGDGVYEIPVGPVHAGMIEPGHFRFSVIGETILRLKARLWFVHRG
VEKLFEGRRPEDAVELAERISGDTAVGHTTAFATAVEDALGIEVPERDRLGRALLLELERMHNHAADLGALANDVGYGIV
NTHALRIRESLLRLNKATTGHRLLRGGVGIGGTRIDALPDPAALEAVAAEVAELAEITLGNSTVRDRFTGTAVLAAESAR
DLGTLGYVARASGIDRDARRDHPFVDLGDRFHVVVETGGDVLARYRVRVREIAVSAAVAGDLVRRLSSGTGVDSHTSAPG
GGHGLGLVEGWRGTIAHRVEIDPSGRLSRVKIVDPSFFNWPALPIALADTIVPDFPLANKSFNQSYAGNDL
>Mature_391_residues
MRHGHWPRGWYPMRRDAGPPPPFDADIGAFPFLEVEGDGVYEIPVGPVHAGMIEPGHFRFSVIGETILRLKARLWFVHRG
VEKLFEGRRPEDAVELAERISGDTAVGHTTAFATAVEDALGIEVPERDRLGRALLLELERMHNHAADLGALANDVGYGIV
NTHALRIRESLLRLNKATTGHRLLRGGVGIGGTRIDALPDPAALEAVAAEVAELAEITLGNSTVRDRFTGTAVLAAESAR
DLGTLGYVARASGIDRDARRDHPFVDLGDRFHVVVETGGDVLARYRVRVREIAVSAAVAGDLVRRLSSGTGVDSHTSAPG
GGHGLGLVEGWRGTIAHRVEIDPSGRLSRVKIVDPSFFNWPALPIALADTIVPDFPLANKSFNQSYAGNDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260898743, Length=267, Percent_Identity=27.3408239700375, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4758786, Length=267, Percent_Identity=27.3408239700375, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789076, Length=399, Percent_Identity=35.3383458646617, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1788832, Length=397, Percent_Identity=32.9974811083123, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI145693162, Length=379, Percent_Identity=24.2744063324538, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17555284, Length=263, Percent_Identity=29.277566539924, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17568379, Length=263, Percent_Identity=28.1368821292776, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221459469, Length=303, Percent_Identity=27.7227722772277, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24638644, Length=253, Percent_Identity=27.6679841897233, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 42148; Mature: 42148

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHGHWPRGWYPMRRDAGPPPPFDADIGAFPFLEVEGDGVYEIPVGPVHAGMIEPGHFRF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCEEE
SVIGETILRLKARLWFVHRGVEKLFEGRRPEDAVELAERISGDTAVGHTTAFATAVEDAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GIEVPERDRLGRALLLELERMHNHAADLGALANDVGYGIVNTHALRIRESLLRLNKATTG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
HRLLRGGVGIGGTRIDALPDPAALEAVAAEVAELAEITLGNSTVRDRFTGTAVLAAESAR
HHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCHH
DLGTLGYVARASGIDRDARRDHPFVDLGDRFHVVVETGGDVLARYRVRVREIAVSAAVAG
HHHHHHHHHHHCCCCCHHHCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
DLVRRLSSGTGVDSHTSAPGGGHGLGLVEGWRGTIAHRVEIDPSGRLSRVKIVDPSFFNW
HHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCC
PALPIALADTIVPDFPLANKSFNQSYAGNDL
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRHGHWPRGWYPMRRDAGPPPPFDADIGAFPFLEVEGDGVYEIPVGPVHAGMIEPGHFRF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCEEE
SVIGETILRLKARLWFVHRGVEKLFEGRRPEDAVELAERISGDTAVGHTTAFATAVEDAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GIEVPERDRLGRALLLELERMHNHAADLGALANDVGYGIVNTHALRIRESLLRLNKATTG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
HRLLRGGVGIGGTRIDALPDPAALEAVAAEVAELAEITLGNSTVRDRFTGTAVLAAESAR
HHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCHH
DLGTLGYVARASGIDRDARRDHPFVDLGDRFHVVVETGGDVLARYRVRVREIAVSAAVAG
HHHHHHHHHHHCCCCCHHHCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
DLVRRLSSGTGVDSHTSAPGGGHGLGLVEGWRGTIAHRVEIDPSGRLSRVKIVDPSFFNW
HHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCC
PALPIALADTIVPDFPLANKSFNQSYAGNDL
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]