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The map label for this gene is hyfF [H]

Identifier: 119718685

GI number: 119718685

Start: 4720175

End: 4721461

Strand: Reverse

Name: hyfF [H]

Synonym: Noca_4466

Alternate gene names: 119718685

Gene position: 4721461-4720175 (Counterclockwise)

Preceding gene: 119718686

Following gene: 119718684

Centisome position: 94.7

GC content: 73.04

Gene sequence:

>1287_bases
GTGGCGCTCGACGACCACACACCGACCGCGCTCGACGGCCTGCTGCGCGTCGACGCCCTCTCGGCGTTCATGCTCACCGT
CATCGGCTCGGTCGGCCTCACCGCGGCCTGGGGAGCGCTGGACCCACTCCGGCCACGGGACCACGTCTACTCGGTGCTCG
CCCTGCTGTTCTTGGGCGCGATGTCGCTCGCGGTGCTCGCCGACAATCTCGGGGTGCTCTGGATCGCCGTCGAGGCGACC
ACCATCGCGACCGCGTTCATGGTCGGTCACCACCGCACCCGTCGCGCGATCGAGGCCGCGTGGAAGTACGTCGTCCTCGG
CTCGGTCGGCGTCGCGATCGCGTTCCTCGGCATCGTGCTGCTCTACGCCGCGACCCGGTCCGCCGGCGAGCCGACCCTGT
CCTGGAGCGTGCTCGCGACCACCCACCCCGACCTGGACCCGTCGGTGGTCCGGGTCGCGGCCGCGCTGGCCATCCTCGGC
TTCGCCACCAAGGCGGGCCTGGCGCCGATGCACAGCTGGCTCCCGGACGCGCACAGTCAGGCGCCCGCACCCGTGTCCGG
GCTGATGTCGGGCGTGCTGCTCGCCGTCGCGTTCTACGCCATCCTCCGGGTGCAGGCCATCGCCACGCCGGTGATCGGCA
CCGGGTTGATGCGCGGACTTCTCGTGACCGCGGGCCTGCTGTCGCTCACCCTCGCCGCGCTCCTGGTGATCCGGCAGCGT
GACCTCAAGCGGATGCTCGCCTACTCCAGCATCGAGCACATGGGGCTGCTCGCGCTGGCCGCCGCCGCCGGCGGCCGGCT
GGCGATCGCGGGCGCCCTGCTGCACGTGCTCGGCCACGGGCTGGCCAAGTCGAGCACGTTCGTGGCCTCCGGCCGGATCC
TCGCGGCGGAGGGCACCACGACGATGGCCGACATCCACGGGCTGGTCGCCCGCCGCCCCGGCGTCGCAGTCCCGTTCCTG
GTCGGCGTGGTGGCGCTGCTCGGGTTGCCGCCCTTCAGCCTGTTCTTCTCCGAGGTGGCGATCGTGGTGGGCGGGGTGCA
GCAGGGACTCGGGTGGGCGATGGCCGTCGCGGTACTGATGCTTCTGGTGATGTTCGTGGCGATGTCGCGCCACGTGGTGG
CGATGCTCTTCGGCGGCGCTGCCGCCGCCACCGACCCGGACCGGGCGCCGTACGGCCCACGCCTCCCGCTCGTGCTCGCG
CTGGCCGCGACCGGCGTCGTGGGCTTCGTCGCCGGGCCCTTCGCCACCCTGCTGGACCGGGCCGCGTCCGTGCTGGGGGG
ATCGTGA

Upstream 100 bases:

>100_bases
GGCCGCCGCCACCGCGATCCTGCGGAACCGGTTCCTCAGCCGGTACGCCGTCGTCGCGGCTGCCGTCGTCGTGCTCGGCT
CGGGCCTGGCCCTGGTGGGC

Downstream 100 bases:

>100_bases
GCACGACGTACGACGTGAGCGCCGCCGACCTGGCACCGACCCTCGGAGCCCGGCTGGCCGACGGCTTCCGGGTGGCGCTG
GTCTCCGGTGTGGAGGACGA

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MALDDHTPTALDGLLRVDALSAFMLTVIGSVGLTAAWGALDPLRPRDHVYSVLALLFLGAMSLAVLADNLGVLWIAVEAT
TIATAFMVGHHRTRRAIEAAWKYVVLGSVGVAIAFLGIVLLYAATRSAGEPTLSWSVLATTHPDLDPSVVRVAAALAILG
FATKAGLAPMHSWLPDAHSQAPAPVSGLMSGVLLAVAFYAILRVQAIATPVIGTGLMRGLLVTAGLLSLTLAALLVIRQR
DLKRMLAYSSIEHMGLLALAAAAGGRLAIAGALLHVLGHGLAKSSTFVASGRILAAEGTTTMADIHGLVARRPGVAVPFL
VGVVALLGLPPFSLFFSEVAIVVGGVQQGLGWAMAVAVLMLLVMFVAMSRHVVAMLFGGAAAATDPDRAPYGPRLPLVLA
LAATGVVGFVAGPFATLLDRAASVLGGS

Sequences:

>Translated_428_residues
MALDDHTPTALDGLLRVDALSAFMLTVIGSVGLTAAWGALDPLRPRDHVYSVLALLFLGAMSLAVLADNLGVLWIAVEAT
TIATAFMVGHHRTRRAIEAAWKYVVLGSVGVAIAFLGIVLLYAATRSAGEPTLSWSVLATTHPDLDPSVVRVAAALAILG
FATKAGLAPMHSWLPDAHSQAPAPVSGLMSGVLLAVAFYAILRVQAIATPVIGTGLMRGLLVTAGLLSLTLAALLVIRQR
DLKRMLAYSSIEHMGLLALAAAAGGRLAIAGALLHVLGHGLAKSSTFVASGRILAAEGTTTMADIHGLVARRPGVAVPFL
VGVVALLGLPPFSLFFSEVAIVVGGVQQGLGWAMAVAVLMLLVMFVAMSRHVVAMLFGGAAAATDPDRAPYGPRLPLVLA
LAATGVVGFVAGPFATLLDRAASVLGGS
>Mature_427_residues
ALDDHTPTALDGLLRVDALSAFMLTVIGSVGLTAAWGALDPLRPRDHVYSVLALLFLGAMSLAVLADNLGVLWIAVEATT
IATAFMVGHHRTRRAIEAAWKYVVLGSVGVAIAFLGIVLLYAATRSAGEPTLSWSVLATTHPDLDPSVVRVAAALAILGF
ATKAGLAPMHSWLPDAHSQAPAPVSGLMSGVLLAVAFYAILRVQAIATPVIGTGLMRGLLVTAGLLSLTLAALLVIRQRD
LKRMLAYSSIEHMGLLALAAAAGGRLAIAGALLHVLGHGLAKSSTFVASGRILAAEGTTTMADIHGLVARRPGVAVPFLV
GVVALLGLPPFSLFFSEVAIVVGGVQQGLGWAMAVAVLMLLVMFVAMSRHVVAMLFGGAAAATDPDRAPYGPRLPLVLAL
AATGVVGFVAGPFATLLDRAASVLGGS

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=325, Percent_Identity=24.9230769230769, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI251831116, Length=228, Percent_Identity=28.5087719298246, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788831, Length=350, Percent_Identity=40.8571428571429, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI1788827, Length=346, Percent_Identity=31.7919075144509, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788613, Length=339, Percent_Identity=26.2536873156342, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788829, Length=378, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI145693160, Length=315, Percent_Identity=27.9365079365079, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788614, Length=346, Percent_Identity=30.3468208092486, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI2367154, Length=431, Percent_Identity=25.5220417633411, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 43860; Mature: 43728

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDDHTPTALDGLLRVDALSAFMLTVIGSVGLTAAWGALDPLRPRDHVYSVLALLFLGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
MSLAVLADNLGVLWIAVEATTIATAFMVGHHRTRRAIEAAWKYVVLGSVGVAIAFLGIVL
HHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYAATRSAGEPTLSWSVLATTHPDLDPSVVRVAAALAILGFATKAGLAPMHSWLPDAHSQ
HHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
APAPVSGLMSGVLLAVAFYAILRVQAIATPVIGTGLMRGLLVTAGLLSLTLAALLVIRQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLKRMLAYSSIEHMGLLALAAAAGGRLAIAGALLHVLGHGLAKSSTFVASGRILAAEGTT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCCEEECCEEEEECCCC
TMADIHGLVARRPGVAVPFLVGVVALLGLPPFSLFFSEVAIVVGGVQQGLGWAMAVAVLM
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVMFVAMSRHVVAMLFGGAAAATDPDRAPYGPRLPLVLALAATGVVGFVAGPFATLLDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AASVLGGS
HHHHHCCC
>Mature Secondary Structure 
ALDDHTPTALDGLLRVDALSAFMLTVIGSVGLTAAWGALDPLRPRDHVYSVLALLFLGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
MSLAVLADNLGVLWIAVEATTIATAFMVGHHRTRRAIEAAWKYVVLGSVGVAIAFLGIVL
HHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYAATRSAGEPTLSWSVLATTHPDLDPSVVRVAAALAILGFATKAGLAPMHSWLPDAHSQ
HHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
APAPVSGLMSGVLLAVAFYAILRVQAIATPVIGTGLMRGLLVTAGLLSLTLAALLVIRQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLKRMLAYSSIEHMGLLALAAAAGGRLAIAGALLHVLGHGLAKSSTFVASGRILAAEGTT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCCEEECCEEEEECCCC
TMADIHGLVARRPGVAVPFLVGVVALLGLPPFSLFFSEVAIVVGGVQQGLGWAMAVAVLM
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVMFVAMSRHVVAMLFGGAAAATDPDRAPYGPRLPLVLALAATGVVGFVAGPFATLLDR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AASVLGGS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]