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The map label for this gene is fadK [H]

Identifier: 119718515

GI number: 119718515

Start: 4538227

End: 4539846

Strand: Reverse

Name: fadK [H]

Synonym: Noca_4296

Alternate gene names: 119718515

Gene position: 4539846-4538227 (Counterclockwise)

Preceding gene: 119718516

Following gene: 119718514

Centisome position: 91.05

GC content: 70.62

Gene sequence:

>1620_bases
ATGACCTTCGAGACGATCCTGACCGCCGAGCGGATCGCCCGGCACACCGCGGACGGCCACTGGCTGGACCGGTTGATCAC
CGACTACCTCGACGAGCACGCCGAGGCGACCCCGGACCGCACCGCGTTCGTCGATGCGCGCCGGGAGATGACGTACGCCG
ACCTGCGCCGCGAGGTCGACCGCTGCGCGCACGGCCTGGTCGAGCTCGGCGTCCGCCCCGGCGACGTGGTCAGCTTCCAG
CTGCCGAACTGGATCGAGTGGGTGGTCGTGCACTACGCGTGCACCCGGATCGGTGCGATCAGCAACCCGCTGATCCCGAT
CTACCGCGAGCGCGAGGTCGGCTTCATGATGGGCCTGGCGCGCTCGCGGGTGCTGGTCGTGCCGCGCCGGTTCCGGGACT
TCGACTACCCCGCGATGGTCGAGCGGCTGCGCCCCGACCTGCCCGATCTCGAGCACGTCCTGGTCGTCGACGGTGACGGT
GCCGACTCGTTCGAGACGTTCCTGGCCACCCCGTGGGAGGAGCGGCACGACCCCGGCCTGCTCGACTCGCTGCGGCCGGA
CGCCAACGACGTCACCCTGCTGATCTTCACCTCCGGCACCACCGGTGAGCCCAAGGGCGTCATGCACACCCACAACACCG
TCGTCGCGGCGAACGCACCGCTGCCCGAGCGCCTCGGCGTCGGCGCGGACAGCGTGATCCACATGGCCTCGACGCTGGCG
CACCTCACCGGCTTCCTGTACGGCGCTCGGCTGCCCGTCCAGGTCGGCGCGACCGGCGTGCTGCAGGACGTGTGGGATCC
GGCCGAGTTCGTGCGGCTCGTCGAGCAGCACCGGATCACCTACACCTCCGCGGCGACGCCGTTCCTCCACGACCTCGTCA
ACGTCGCCGACCTCGCCGACCACGATCTGTCCAGCCTGCAGCGGTTCTGCTGCATGGGCGCCCCGATCCCGCGGGCCATC
GTCCGCCGCGCGCGGGCCGCGCTGCCGGGCCTGGTGGTGGTCGGTGGCTGGGGCCAGACCGAGAACGCGCTCGTCACGCT
CGGCGTCCCCGGCGACCCGGACGAGAAGCTGATCGAGACCGACGGCTACCCCTGGCCGGGCATGCGGATCCGGGTCGTCG
ACGCCGACGGTGCCGAGGTGCCGGCGGGCACCGAGGGCCGGCTCCAGGTGACCGGCCCCTTCCTGTTCGTCGGGTACGCC
GAGCGCCTCGAGATGACCCGCGACTCCTTCGACGGCGACTGGTTCGACACCGGCGACCTGGCCCACATCGACGCCGAGGG
CTACGTGAGCATCTCCGGGCGCACCAAGGACGTGATCATCCGCGGCGGCGAGAACATCCCCGTCGCCTACGTCGAGAACG
TGCTGTACGAGAACCCCAAGATCGAGGTGGTGGCCGTGGTGGCGGTCCCCGACCCGCGACTCCAGGAACGGGCGTGCGCC
TGCGTCGTGCTGAAGCCGGGCGTGGCCGGTTTCGACTTCGCCGAGATGCAGGAGTTCCTGCGGGAGAAGGGAGTGGCGAA
GCAGTACTGGCCGGAGTTCCTCACCGTCCTGCCCGAGCTCCCCAAGACCCCCAGCGGCAAGATCCAGAAGTTCCACCTGC
GCGACCAGGTGGCCCAATGA

Upstream 100 bases:

>100_bases
GTGGTCGCCTTCCTGGCCTCCGACGAGGCCGGCTTCGTCACCGGACAGACCGTCAGCGTCAGCGGCGGCCTCACGATGTC
TTGACCACGGGAGACCTGCC

Downstream 100 bases:

>100_bases
GCGCCGGGTTCGAGACGCTGCTGGTCGAGGTCGCCGACGGGATCGCAGTGGTGACCGTCAACCGCCCGGAGGTCCGCAAT
GCGGTGAGCCGGCAGGTCCA

Product: cyclohexanecarboxylate-CoA ligase

Products: crotonoyl-CoA; CO2; reduced acceptor

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTFETILTAERIARHTADGHWLDRLITDYLDEHAEATPDRTAFVDARREMTYADLRREVDRCAHGLVELGVRPGDVVSFQ
LPNWIEWVVVHYACTRIGAISNPLIPIYREREVGFMMGLARSRVLVVPRRFRDFDYPAMVERLRPDLPDLEHVLVVDGDG
ADSFETFLATPWEERHDPGLLDSLRPDANDVTLLIFTSGTTGEPKGVMHTHNTVVAANAPLPERLGVGADSVIHMASTLA
HLTGFLYGARLPVQVGATGVLQDVWDPAEFVRLVEQHRITYTSAATPFLHDLVNVADLADHDLSSLQRFCCMGAPIPRAI
VRRARAALPGLVVVGGWGQTENALVTLGVPGDPDEKLIETDGYPWPGMRIRVVDADGAEVPAGTEGRLQVTGPFLFVGYA
ERLEMTRDSFDGDWFDTGDLAHIDAEGYVSISGRTKDVIIRGGENIPVAYVENVLYENPKIEVVAVVAVPDPRLQERACA
CVVLKPGVAGFDFAEMQEFLREKGVAKQYWPEFLTVLPELPKTPSGKIQKFHLRDQVAQ

Sequences:

>Translated_539_residues
MTFETILTAERIARHTADGHWLDRLITDYLDEHAEATPDRTAFVDARREMTYADLRREVDRCAHGLVELGVRPGDVVSFQ
LPNWIEWVVVHYACTRIGAISNPLIPIYREREVGFMMGLARSRVLVVPRRFRDFDYPAMVERLRPDLPDLEHVLVVDGDG
ADSFETFLATPWEERHDPGLLDSLRPDANDVTLLIFTSGTTGEPKGVMHTHNTVVAANAPLPERLGVGADSVIHMASTLA
HLTGFLYGARLPVQVGATGVLQDVWDPAEFVRLVEQHRITYTSAATPFLHDLVNVADLADHDLSSLQRFCCMGAPIPRAI
VRRARAALPGLVVVGGWGQTENALVTLGVPGDPDEKLIETDGYPWPGMRIRVVDADGAEVPAGTEGRLQVTGPFLFVGYA
ERLEMTRDSFDGDWFDTGDLAHIDAEGYVSISGRTKDVIIRGGENIPVAYVENVLYENPKIEVVAVVAVPDPRLQERACA
CVVLKPGVAGFDFAEMQEFLREKGVAKQYWPEFLTVLPELPKTPSGKIQKFHLRDQVAQ
>Mature_538_residues
TFETILTAERIARHTADGHWLDRLITDYLDEHAEATPDRTAFVDARREMTYADLRREVDRCAHGLVELGVRPGDVVSFQL
PNWIEWVVVHYACTRIGAISNPLIPIYREREVGFMMGLARSRVLVVPRRFRDFDYPAMVERLRPDLPDLEHVLVVDGDGA
DSFETFLATPWEERHDPGLLDSLRPDANDVTLLIFTSGTTGEPKGVMHTHNTVVAANAPLPERLGVGADSVIHMASTLAH
LTGFLYGARLPVQVGATGVLQDVWDPAEFVRLVEQHRITYTSAATPFLHDLVNVADLADHDLSSLQRFCCMGAPIPRAIV
RRARAALPGLVVVGGWGQTENALVTLGVPGDPDEKLIETDGYPWPGMRIRVVDADGAEVPAGTEGRLQVTGPFLFVGYAE
RLEMTRDSFDGDWFDTGDLAHIDAEGYVSISGRTKDVIIRGGENIPVAYVENVLYENPKIEVVAVVAVPDPRLQERACAC
VVLKPGVAGFDFAEMQEFLREKGVAKQYWPEFLTVLPELPKTPSGKIQKFHLRDQVAQ

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It has a low activity on medium or long chain fatty acids and is ma

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein. Note=Partially membrane-associated [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=550, Percent_Identity=29.4545454545455, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI38505220, Length=527, Percent_Identity=27.3244781783681, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI42544132, Length=537, Percent_Identity=25.6983240223464, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=25.1968503937008, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI187761345, Length=541, Percent_Identity=27.3567467652495, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI187761343, Length=541, Percent_Identity=27.3567467652495, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI122937307, Length=491, Percent_Identity=26.0692464358452, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI58082049, Length=483, Percent_Identity=25.2587991718427, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI157311624, Length=483, Percent_Identity=25.0517598343685, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157311622, Length=483, Percent_Identity=25.0517598343685, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI28416953, Length=546, Percent_Identity=24.7252747252747, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI13236579, Length=498, Percent_Identity=26.1044176706827, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI8923896, Length=373, Percent_Identity=27.8820375335121, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI253970489, Length=373, Percent_Identity=27.8820375335121, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI145693145, Length=539, Percent_Identity=35.9925788497217, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=27.6119402985075, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=26.4492753623188, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790505, Length=554, Percent_Identity=27.9783393501805, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=26.0355029585799, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786801, Length=491, Percent_Identity=25.2545824847251, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786529, Length=543, Percent_Identity=25.7826887661142, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789201, Length=361, Percent_Identity=26.5927977839335, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=540, Percent_Identity=25.9259259259259, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71994690, Length=540, Percent_Identity=25.9259259259259, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71994694, Length=540, Percent_Identity=25.9259259259259, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17560308, Length=539, Percent_Identity=29.6846011131725, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17538037, Length=530, Percent_Identity=26.6037735849057, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=543, Percent_Identity=25.7826887661142, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=543, Percent_Identity=25.0460405156538, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=507, Percent_Identity=25.2465483234714, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71996755, Length=523, Percent_Identity=23.5181644359465, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17558820, Length=501, Percent_Identity=24.1516966067864, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=535, Percent_Identity=22.4299065420561, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17507435, Length=534, Percent_Identity=21.5355805243446, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=550, Percent_Identity=23.0909090909091, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=538, Percent_Identity=26.0223048327138, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71983001, Length=538, Percent_Identity=26.0223048327138, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=518, Percent_Identity=21.2355212355212, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323182, Length=555, Percent_Identity=24.5045045045045, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319264, Length=552, Percent_Identity=25.9057971014493, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319699, Length=471, Percent_Identity=21.8683651804671, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319591, Length=614, Percent_Identity=22.9641693811075, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24581924, Length=535, Percent_Identity=28.2242990654206, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI21355181, Length=539, Percent_Identity=24.6753246753247, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI18859661, Length=502, Percent_Identity=25.4980079681275, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI62472339, Length=532, Percent_Identity=25.3759398496241, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24667955, Length=532, Percent_Identity=25.3759398496241, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281365686, Length=465, Percent_Identity=25.3763440860215, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24656500, Length=506, Percent_Identity=23.1225296442688, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356441, Length=446, Percent_Identity=26.0089686098655, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24653035, Length=480, Percent_Identity=24.5833333333333, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922652, Length=520, Percent_Identity=24.4230769230769, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI20130357, Length=379, Percent_Identity=24.802110817942, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21358303, Length=474, Percent_Identity=25.3164556962025, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356947, Length=428, Percent_Identity=24.0654205607477, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI161076582, Length=480, Percent_Identity=23.5416666666667, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24648253, Length=504, Percent_Identity=23.8095238095238, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24667959, Length=366, Percent_Identity=27.3224043715847, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648255, Length=504, Percent_Identity=24.0079365079365, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17933690, Length=497, Percent_Identity=23.7424547283702, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648257, Length=464, Percent_Identity=23.4913793103448, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648260, Length=481, Percent_Identity=23.2848232848233, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 1.3.99.7

Molecular weight: Translated: 59726; Mature: 59594

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFETILTAERIARHTADGHWLDRLITDYLDEHAEATPDRTAFVDARREMTYADLRREVD
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
RCAHGLVELGVRPGDVVSFQLPNWIEWVVVHYACTRIGAISNPLIPIYREREVGFMMGLA
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHH
RSRVLVVPRRFRDFDYPAMVERLRPDLPDLEHVLVVDGDGADSFETFLATPWEERHDPGL
CCCEEEECHHHCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHCCCCCC
LDSLRPDANDVTLLIFTSGTTGEPKGVMHTHNTVVAANAPLPERLGVGADSVIHMASTLA
HHHCCCCCCCEEEEEEECCCCCCCCCEEEECCEEEEECCCCHHHHCCCHHHHHHHHHHHH
HLTGFLYGARLPVQVGATGVLQDVWDPAEFVRLVEQHRITYTSAATPFLHDLVNVADLAD
HHHHHHHCCCCCEEECCCHHHHHHCCHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHCC
HDLSSLQRFCCMGAPIPRAIVRRARAALPGLVVVGGWGQTENALVTLGVPGDPDEKLIET
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCHHEEC
DGYPWPGMRIRVVDADGAEVPAGTEGRLQVTGPFLFVGYAERLEMTRDSFDGDWFDTGDL
CCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHCCCCCCCCCCCE
AHIDAEGYVSISGRTKDVIIRGGENIPVAYVENVLYENPKIEVVAVVAVPDPRLQERACA
EEECCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHCCCEE
CVVLKPGVAGFDFAEMQEFLREKGVAKQYWPEFLTVLPELPKTPSGKIQKFHLRDQVAQ
EEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCC
>Mature Secondary Structure 
TFETILTAERIARHTADGHWLDRLITDYLDEHAEATPDRTAFVDARREMTYADLRREVD
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
RCAHGLVELGVRPGDVVSFQLPNWIEWVVVHYACTRIGAISNPLIPIYREREVGFMMGLA
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHH
RSRVLVVPRRFRDFDYPAMVERLRPDLPDLEHVLVVDGDGADSFETFLATPWEERHDPGL
CCCEEEECHHHCCCCCHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHCCCCCC
LDSLRPDANDVTLLIFTSGTTGEPKGVMHTHNTVVAANAPLPERLGVGADSVIHMASTLA
HHHCCCCCCCEEEEEEECCCCCCCCCEEEECCEEEEECCCCHHHHCCCHHHHHHHHHHHH
HLTGFLYGARLPVQVGATGVLQDVWDPAEFVRLVEQHRITYTSAATPFLHDLVNVADLAD
HHHHHHHCCCCCEEECCCHHHHHHCCHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHCC
HDLSSLQRFCCMGAPIPRAIVRRARAALPGLVVVGGWGQTENALVTLGVPGDPDEKLIET
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCHHEEC
DGYPWPGMRIRVVDADGAEVPAGTEGRLQVTGPFLFVGYAERLEMTRDSFDGDWFDTGDL
CCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCEEEEEHHHHHHHHHHCCCCCCCCCCCE
AHIDAEGYVSISGRTKDVIIRGGENIPVAYVENVLYENPKIEVVAVVAVPDPRLQERACA
EEECCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHCCCEE
CVVLKPGVAGFDFAEMQEFLREKGVAKQYWPEFLTVLPELPKTPSGKIQKFHLRDQVAQ
EEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glutaryl-CoA; acceptor

Specific reaction: glutaryl-CoA + acceptor = crotonoyl-CoA + CO2 + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 7984428; 12535077 [H]