BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is yngF [H]

Identifier: 119718514

GI number: 119718514

Start: 4537448

End: 4538230

Strand: Reverse

Name: yngF [H]

Synonym: Noca_4295

Alternate gene names: 119718514

Gene position: 4538230-4537448 (Counterclockwise)

Preceding gene: 119718515

Following gene: 119718513

Centisome position: 91.02

GC content: 73.05

Gene sequence:

>783_bases
ATGAGCGCCGGGTTCGAGACGCTGCTGGTCGAGGTCGCCGACGGGATCGCAGTGGTGACCGTCAACCGCCCGGAGGTCCG
CAATGCGGTGAGCCGGCAGGTCCAGGCCGACCTCCGCGCCGTCCTCGACACGTTCCGTCACGACGACGCGGTGGAGGTGG
TGGTGTTCACCGGCGCGGGCGACCGCGCCTTCGTCGCCGGCGCCGACATCGCCCAGCTGCGCGACTACACGCTGCACACC
GGACTCGCCTCGGAGATGCAGGCGCTCTACGACGAGGTCGAGGCCTATGAGAAGCCGACCATCGCCGCGGTCAACGGCTA
CGCCCTGGGCGGTGGCTGCGAGCTCGCGATGGCGTGCGACCTGCGGGTGGCGTCCACGAGCGCCCGGTTCGGGCTGCCGG
AGACCAACCTCGCCGTACTGCCCGGCGCGGGCGGCACCCAGCGGCTCGCCAGGCTGGTCGGCGTGGGGCGTGCCCTCGAG
CTGATCCTCACCGGACGGCTCGTCGACGCCGAGGAGGCCCGCACGATCGGGCTGGTCACCTCGGTGGTCGCACCGGAGGA
GCTCCTCCCCCACGCCCGCGAGGTGGCCGGGCAGATCCGGGCCAAGGGTCCGCTCGCGGTCCGGCTCGCCAAGCTGGTGG
TGCGCTCCGGCATGGACACCGACCGGCGGACCGGCCTGGTCATCGAGCAGCTGGCCCAGTCGCTGCTCTACACCACCGGG
GACAAGCGGGAGGGCGCCGAGGCGTTCCTGGCCAAGCGCACCCCTGCGTTCCGGGGGGCCTGA

Upstream 100 bases:

>100_bases
CGAAGCAGTACTGGCCGGAGTTCCTCACCGTCCTGCCCGAGCTCCCCAAGACCCCCAGCGGCAAGATCCAGAAGTTCCAC
CTGCGCGACCAGGTGGCCCA

Downstream 100 bases:

>100_bases
TGCGCAGCGTCCTGGTCGTGGGTGCGGGGGCGATGGGCTCCCAGATCGCGATGGTCTGCGCCCTCGCGGGCCACCAGGTG
TGCCTCCACGACGTCGATCC

Product: short chain enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSAGFETLLVEVADGIAVVTVNRPEVRNAVSRQVQADLRAVLDTFRHDDAVEVVVFTGAGDRAFVAGADIAQLRDYTLHT
GLASEMQALYDEVEAYEKPTIAAVNGYALGGGCELAMACDLRVASTSARFGLPETNLAVLPGAGGTQRLARLVGVGRALE
LILTGRLVDAEEARTIGLVTSVVAPEELLPHAREVAGQIRAKGPLAVRLAKLVVRSGMDTDRRTGLVIEQLAQSLLYTTG
DKREGAEAFLAKRTPAFRGA

Sequences:

>Translated_260_residues
MSAGFETLLVEVADGIAVVTVNRPEVRNAVSRQVQADLRAVLDTFRHDDAVEVVVFTGAGDRAFVAGADIAQLRDYTLHT
GLASEMQALYDEVEAYEKPTIAAVNGYALGGGCELAMACDLRVASTSARFGLPETNLAVLPGAGGTQRLARLVGVGRALE
LILTGRLVDAEEARTIGLVTSVVAPEELLPHAREVAGQIRAKGPLAVRLAKLVVRSGMDTDRRTGLVIEQLAQSLLYTTG
DKREGAEAFLAKRTPAFRGA
>Mature_259_residues
SAGFETLLVEVADGIAVVTVNRPEVRNAVSRQVQADLRAVLDTFRHDDAVEVVVFTGAGDRAFVAGADIAQLRDYTLHTG
LASEMQALYDEVEAYEKPTIAAVNGYALGGGCELAMACDLRVASTSARFGLPETNLAVLPGAGGTQRLARLVGVGRALEL
ILTGRLVDAEEARTIGLVTSVVAPEELLPHAREVAGQIRAKGPLAVRLAKLVVRSGMDTDRRTGLVIEQLAQSLLYTTGD
KREGAEAFLAKRTPAFRGA

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=37.4517374517375, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=37.3540856031128, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=37.7777777777778, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=34.8, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI68989263, Length=188, Percent_Identity=38.2978723404255, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=29.7619047619048, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI70995211, Length=226, Percent_Identity=30.5309734513274, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI45643119, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260275230, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260274832, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=29.7872340425532, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=29.7872340425532, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI261878539, Length=94, Percent_Identity=47.8723404255319, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI157694516, Length=211, Percent_Identity=28.436018957346, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI213417737, Length=211, Percent_Identity=28.436018957346, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI221136756, Length=228, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=228, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=225, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI25453481, Length=228, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4757966, Length=228, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI51538573, Length=228, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI51538581, Length=228, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI116235487, Length=248, Percent_Identity=25.4032258064516, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=204, Percent_Identity=25.9803921568627, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI49355787, Length=204, Percent_Identity=25.9803921568627, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI62530384, Length=222, Percent_Identity=27.9279279279279, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI157694520, Length=122, Percent_Identity=33.6065573770492, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=40.8730158730159, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=34.469696969697, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=34.5864661654135, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788682, Length=215, Percent_Identity=41.3953488372093, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1790281, Length=203, Percent_Identity=36.4532019704434, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=34.469696969697, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082183, Length=265, Percent_Identity=27.9245283018868, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=38.1147540983607, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17540714, Length=219, Percent_Identity=39.7260273972603, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=38.6178861788618, Blast_Score=166, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=35.1648351648352, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=37.3626373626374, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17508951, Length=226, Percent_Identity=33.6283185840708, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17508953, Length=226, Percent_Identity=33.6283185840708, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=173, Percent_Identity=34.6820809248555, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=29.4117647058824, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=208, Percent_Identity=28.8461538461538, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=280, Percent_Identity=26.4285714285714, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=172, Percent_Identity=26.7441860465116, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=227, Percent_Identity=26.8722466960352, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=27.4611398963731, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=39.84375, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=39.84375, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=40.0763358778626, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=25.3968253968254, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357171, Length=195, Percent_Identity=34.8717948717949, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24650670, Length=209, Percent_Identity=35.8851674641148, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19921000, Length=184, Percent_Identity=35.8695652173913, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583077, Length=184, Percent_Identity=35.8695652173913, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583079, Length=184, Percent_Identity=35.8695652173913, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24654903, Length=208, Percent_Identity=27.4038461538462, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19920382, Length=243, Percent_Identity=29.2181069958848, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19921018, Length=220, Percent_Identity=29.5454545454545, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583165, Length=238, Percent_Identity=31.0924369747899, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571730, Length=185, Percent_Identity=29.1891891891892, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571729, Length=185, Percent_Identity=29.1891891891892, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 27524; Mature: 27393

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGFETLLVEVADGIAVVTVNRPEVRNAVSRQVQADLRAVLDTFRHDDAVEVVVFTGAG
CCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DRAFVAGADIAQLRDYTLHTGLASEMQALYDEVEAYEKPTIAAVNGYALGGGCELAMACD
CCEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCEEEEEEC
LRVASTSARFGLPETNLAVLPGAGGTQRLARLVGVGRALELILTGRLVDAEEARTIGLVT
EEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHH
SVVAPEELLPHAREVAGQIRAKGPLAVRLAKLVVRSGMDTDRRTGLVIEQLAQSLLYTTG
HHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
DKREGAEAFLAKRTPAFRGA
CCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAGFETLLVEVADGIAVVTVNRPEVRNAVSRQVQADLRAVLDTFRHDDAVEVVVFTGAG
CCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
DRAFVAGADIAQLRDYTLHTGLASEMQALYDEVEAYEKPTIAAVNGYALGGGCELAMACD
CCEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCEEEEEEC
LRVASTSARFGLPETNLAVLPGAGGTQRLARLVGVGRALELILTGRLVDAEEARTIGLVT
EEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHH
SVVAPEELLPHAREVAGQIRAKGPLAVRLAKLVVRSGMDTDRRTGLVIEQLAQSLLYTTG
HHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
DKREGAEAFLAKRTPAFRGA
CCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]