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The map label for this gene is menE [H]

Identifier: 119718335

GI number: 119718335

Start: 4346531

End: 4348030

Strand: Reverse

Name: menE [H]

Synonym: Noca_4116

Alternate gene names: 119718335

Gene position: 4348030-4346531 (Counterclockwise)

Preceding gene: 119718336

Following gene: 119718334

Centisome position: 87.21

GC content: 71.4

Gene sequence:

>1500_bases
ATGGACTACACGCTGGCCGACATGGTCGGCATGGTCGCCCGCAGTCGCCCCGACCAGGTGGCGGCGACGGTGCTCGGCGA
GGAGACGACGACGTACTCGTTCGCCGAGCTCTGGGACCGGATCGGCGACCTGGCTGACGCGGTCTCCGACACGGAGGCCG
GCGTCCACGGACCGATGGTCGCGACCCTGCTGCCGAACAGCCTCGACGCGCTGGCCGCCTACCTGGCGTGCCAGCGCGGC
GGGGTCGGCGCGGTGCCGCTCAACAACCGGCTCGCCGACCGCGAGCTCCAGCACATCCTGGCGGACTCGGAGGCGCGAAC
CATCCTGGCCGCCGGCGAGTACGTCGAGGTCGCGCGGCGGATCGCCGACGCCGGCGTGCGCGTGGTCGACGCGGACACGG
TCGTGGCGGGCAGCCGCGACCGCGACCTGATCGCCGATCCCGAGCGGGGCAGCCGGGTGGCCGTCGTGTTCTACACGTCC
GGCACCACGGGCCTGCCCAAGGGCGCCGCCGTGCGCAACGACGACTGGGTGGTCAACACGATGCGCTGGGGGTGGCAGCT
GCGGATCCAGTGGGACGAGCGGACGCTCGTCCCCGGCCCGCTGTTCCACATGTCCTACTCGAGCTTCGCGCTGGCCACCT
GGCTGATGGGTGGCGAGGTCCGGATCATGCCGTCGTTCTCGGCGGCCACCGCCTACGAGGAGTTCGCCGAGCGCTCGACG
TTCGCGTTCCTGGTGCCGTCGATGACCCAGATGATCCACGACGAGTGGGTCGCCCGTGGCCGGCAGCCGATGCCGGCGGC
GCGTTCGATCATGACCTCGGGAGCCGCGGCCGCGGTCGAGCTGGTCGAGGCGGCATTCGACATGTTCCCGAACGCGACCA
TCCAGGAGACCTACGGGTGGACCGAGGCGGGCTTCGCGACGATGGAGGTCAAGACCCGCGAGACCGTCCGCCGCGGCACG
GTCGGCTACAACACCGTCGGGTCAGACGTCGCCGTGTTCGACGACGACGGCAAGCCGTGCGCGCCGGGCGAGCGGGGTGA
GGTCGGAATCCGCACCCTCGCGGCCTCGATCGGCTACCTCTCGCCGGCGACCGCGGGGGCGAACACCCGCCGCGGACCGT
GGATCCTCTCGGGCGACATCGGGACGTTCGACGAGGCCGGTCGCCTGATCATCATCGACCGCAAGCACGGCATGATCATC
AGCGGCGGGGAGAACGTCTACGCCGCCGAGGTCGAGAAGGTCGTGGGCCAGCACCCCGCCGTCGACGAGTGCGTCGTGAT
CGGCCGCCCGAGTCGGCAGTGGGGCGAGGAGATCGTCGCCGTCGTGGTCCTCGAGACCGGCGCCGACCTGGACCTCGACG
GCCTGCGCGTGTTCTGCCGCGAGCACCTCGCCTCGTACAAGGTCCCGCGCGACCTGGCGATCGTCGACGCGCTCCCCCGG
AACTCGATGGGCAAGCTCCAGCGGTTCGAGGTCAAGCGGGTCCTGGAGGCGACGGGGTGA

Upstream 100 bases:

>100_bases
CTCTCCTTCGACGATGCCGGGTTGAACACCAGCCCGATGGGCCTGGGCATCCTCCGGGACGGGCAAGGCGAGGTCGTGTC
GTTCGGGGGGCGGTGACCCA

Downstream 100 bases:

>100_bases
GCGACCTCCTGCTGACCCTCCTGCTCGGCCTCATGCAGGCGGGCCTCTACACGCTCGTCGGCCTGGGCCTGACGATCACC
TTCGGCGTGACCCACATCCT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MDYTLADMVGMVARSRPDQVAATVLGEETTTYSFAELWDRIGDLADAVSDTEAGVHGPMVATLLPNSLDALAAYLACQRG
GVGAVPLNNRLADRELQHILADSEARTILAAGEYVEVARRIADAGVRVVDADTVVAGSRDRDLIADPERGSRVAVVFYTS
GTTGLPKGAAVRNDDWVVNTMRWGWQLRIQWDERTLVPGPLFHMSYSSFALATWLMGGEVRIMPSFSAATAYEEFAERST
FAFLVPSMTQMIHDEWVARGRQPMPAARSIMTSGAAAAVELVEAAFDMFPNATIQETYGWTEAGFATMEVKTRETVRRGT
VGYNTVGSDVAVFDDDGKPCAPGERGEVGIRTLAASIGYLSPATAGANTRRGPWILSGDIGTFDEAGRLIIIDRKHGMII
SGGENVYAAEVEKVVGQHPAVDECVVIGRPSRQWGEEIVAVVVLETGADLDLDGLRVFCREHLASYKVPRDLAIVDALPR
NSMGKLQRFEVKRVLEATG

Sequences:

>Translated_499_residues
MDYTLADMVGMVARSRPDQVAATVLGEETTTYSFAELWDRIGDLADAVSDTEAGVHGPMVATLLPNSLDALAAYLACQRG
GVGAVPLNNRLADRELQHILADSEARTILAAGEYVEVARRIADAGVRVVDADTVVAGSRDRDLIADPERGSRVAVVFYTS
GTTGLPKGAAVRNDDWVVNTMRWGWQLRIQWDERTLVPGPLFHMSYSSFALATWLMGGEVRIMPSFSAATAYEEFAERST
FAFLVPSMTQMIHDEWVARGRQPMPAARSIMTSGAAAAVELVEAAFDMFPNATIQETYGWTEAGFATMEVKTRETVRRGT
VGYNTVGSDVAVFDDDGKPCAPGERGEVGIRTLAASIGYLSPATAGANTRRGPWILSGDIGTFDEAGRLIIIDRKHGMII
SGGENVYAAEVEKVVGQHPAVDECVVIGRPSRQWGEEIVAVVVLETGADLDLDGLRVFCREHLASYKVPRDLAIVDALPR
NSMGKLQRFEVKRVLEATG
>Mature_499_residues
MDYTLADMVGMVARSRPDQVAATVLGEETTTYSFAELWDRIGDLADAVSDTEAGVHGPMVATLLPNSLDALAAYLACQRG
GVGAVPLNNRLADRELQHILADSEARTILAAGEYVEVARRIADAGVRVVDADTVVAGSRDRDLIADPERGSRVAVVFYTS
GTTGLPKGAAVRNDDWVVNTMRWGWQLRIQWDERTLVPGPLFHMSYSSFALATWLMGGEVRIMPSFSAATAYEEFAERST
FAFLVPSMTQMIHDEWVARGRQPMPAARSIMTSGAAAAVELVEAAFDMFPNATIQETYGWTEAGFATMEVKTRETVRRGT
VGYNTVGSDVAVFDDDGKPCAPGERGEVGIRTLAASIGYLSPATAGANTRRGPWILSGDIGTFDEAGRLIIIDRKHGMII
SGGENVYAAEVEKVVGQHPAVDECVVIGRPSRQWGEEIVAVVVLETGADLDLDGLRVFCREHLASYKVPRDLAIVDALPR
NSMGKLQRFEVKRVLEATG

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=547, Percent_Identity=25.7769652650823, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI187761345, Length=506, Percent_Identity=27.6679841897233, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI187761343, Length=506, Percent_Identity=27.6679841897233, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI58082049, Length=489, Percent_Identity=25.9713701431493, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI157311624, Length=489, Percent_Identity=25.9713701431493, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI157311622, Length=489, Percent_Identity=25.9713701431493, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI42544132, Length=516, Percent_Identity=23.4496124031008, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI115511026, Length=501, Percent_Identity=25.748502994012, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI122937307, Length=493, Percent_Identity=24.1379310344828, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI38505220, Length=506, Percent_Identity=22.9249011857708, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI145693145, Length=520, Percent_Identity=26.1538461538462, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=25.6029684601113, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI221142682, Length=490, Percent_Identity=25.5102040816327, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786810, Length=416, Percent_Identity=24.5192307692308, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786801, Length=523, Percent_Identity=22.9445506692161, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788595, Length=361, Percent_Identity=24.3767313019391, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI32563687, Length=477, Percent_Identity=27.6729559748428, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17558820, Length=477, Percent_Identity=26.8343815513627, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17560308, Length=376, Percent_Identity=27.6595744680851, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=25.2427184466019, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=479, Percent_Identity=25.678496868476, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=419, Percent_Identity=25.5369928400955, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=419, Percent_Identity=25.5369928400955, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=419, Percent_Identity=25.5369928400955, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=478, Percent_Identity=24.6861924686192, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=339, Percent_Identity=27.1386430678466, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=515, Percent_Identity=25.0485436893204, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=486, Percent_Identity=23.6625514403292, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=23.1182795698925, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=514, Percent_Identity=26.0700389105058, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24581924, Length=532, Percent_Identity=26.3157894736842, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI18859661, Length=497, Percent_Identity=27.364185110664, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21355181, Length=458, Percent_Identity=23.5807860262009, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24648253, Length=511, Percent_Identity=26.8101761252446, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648255, Length=511, Percent_Identity=26.614481409002, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI20130357, Length=354, Percent_Identity=25.1412429378531, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19922652, Length=526, Percent_Identity=23.0038022813688, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653035, Length=471, Percent_Identity=22.5053078556263, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356947, Length=466, Percent_Identity=23.175965665236, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21356441, Length=499, Percent_Identity=22.6452905811623, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648257, Length=358, Percent_Identity=25.4189944134078, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI161076582, Length=469, Percent_Identity=21.7484008528785, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24656500, Length=380, Percent_Identity=26.0526315789474, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI221329755, Length=93, Percent_Identity=30.1075268817204, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53990; Mature: 53990

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00455 AMP_BINDING ; PS00309 GALAPTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTLADMVGMVARSRPDQVAATVLGEETTTYSFAELWDRIGDLADAVSDTEAGVHGPMV
CCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
ATLLPNSLDALAAYLACQRGGVGAVPLNNRLADRELQHILADSEARTILAAGEYVEVARR
EECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
IADAGVRVVDADTVVAGSRDRDLIADPERGSRVAVVFYTSGTTGLPKGAAVRNDDWVVNT
HHHCCCEEEECCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEECCCCEEEEE
MRWGWQLRIQWDERTLVPGPLFHMSYSSFALATWLMGGEVRIMPSFSAATAYEEFAERST
EECCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCC
FAFLVPSMTQMIHDEWVARGRQPMPAARSIMTSGAAAAVELVEAAFDMFPNATIQETYGW
EEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHCCCC
TEAGFATMEVKTRETVRRGTVGYNTVGSDVAVFDDDGKPCAPGERGEVGIRTLAASIGYL
CCCCEEEEEEHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
SPATAGANTRRGPWILSGDIGTFDEAGRLIIIDRKHGMIISGGENVYAAEVEKVVGQHPA
CCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEECCCCEEHHHHHHHHCCCCC
VDECVVIGRPSRQWGEEIVAVVVLETGADLDLDGLRVFCREHLASYKVPRDLAIVDALPR
CCCEEEECCCCHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
NSMGKLQRFEVKRVLEATG
CCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDYTLADMVGMVARSRPDQVAATVLGEETTTYSFAELWDRIGDLADAVSDTEAGVHGPMV
CCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
ATLLPNSLDALAAYLACQRGGVGAVPLNNRLADRELQHILADSEARTILAAGEYVEVARR
EECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
IADAGVRVVDADTVVAGSRDRDLIADPERGSRVAVVFYTSGTTGLPKGAAVRNDDWVVNT
HHHCCCEEEECCEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEECCCCEEEEE
MRWGWQLRIQWDERTLVPGPLFHMSYSSFALATWLMGGEVRIMPSFSAATAYEEFAERST
EECCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCC
FAFLVPSMTQMIHDEWVARGRQPMPAARSIMTSGAAAAVELVEAAFDMFPNATIQETYGW
EEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEHHCCCC
TEAGFATMEVKTRETVRRGTVGYNTVGSDVAVFDDDGKPCAPGERGEVGIRTLAASIGYL
CCCCEEEEEEHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
SPATAGANTRRGPWILSGDIGTFDEAGRLIIIDRKHGMIISGGENVYAAEVEKVVGQHPA
CCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEECCCCEEHHHHHHHHCCCCC
VDECVVIGRPSRQWGEEIVAVVVLETGADLDLDGLRVFCREHLASYKVPRDLAIVDALPR
CCCEEEECCCCHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
NSMGKLQRFEVKRVLEATG
CCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA