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The map label for this gene is trmB

Identifier: 119718139

GI number: 119718139

Start: 4125444

End: 4126190

Strand: Reverse

Name: trmB

Synonym: Noca_3919

Alternate gene names: 119718139

Gene position: 4126190-4125444 (Counterclockwise)

Preceding gene: 119718142

Following gene: 119718137

Centisome position: 82.76

GC content: 75.23

Gene sequence:

>747_bases
ATGCCCGAACACGCCCCCGACGGGGTGCGCCCCGCGCGCCCGCACCACAAGCTCACGGCGGACGGGCGGCGGATGCGGGA
GGTGCTCACCTACTCGCGTCGGGGCAGCCGGTTCTCGCCTCGTCAGCAGCAGGCCTGGGCCGCGTACGCCGAGCGCTGGT
GGGTGCCCGACGAGGCGGTCGACGACCCCGGCTTCACCCTGGCCGGCTGCTTCGAGCGCAGCGCGCCCCTCATCGTCGAG
ATCGGCTCGGGCATCGGCGAGTCGACGGTCGCCCTGGCGGCGGCGCGGCCCGACCACGACGTGGTCGCGATCGAGGTCTG
GCGGCCCGGGGTCGCGGACACGCTCGGCCGGATCGGCGCGGCCGGCCTGTCCAACGTCCGGCTGCTCAGCGTGGACGCCG
TGTGGTCGATGGCGCACCTGGTGGAGCCCGACAGCCTCGCGGCGCTCTGGACCTTCTTCCCCGACCCCTGGCACAAGGCG
AAGCACCACAAGCGACGCCTGGTCACCGCGTCCTTCGCCCGGCTCGTCGCGGGGCGGCTGGCGCCCGGTGCCGAGTGGCG
GCTGGCGACCGACTGGGCCGACTACGCCGACCAGATGGTCGAGGTGCTCGACGCCGAGCCGATGCTCGAGGGCGGCGTCG
TGGAGCGGTGGGCGGAGCGTCCGGTGACGAAGTTCGAGCGCAAGGGGATCGCGGCCGGGCGCGCGATCACCGACCTCGCC
TACCGCCGCCGCGGCGCCGCGCGCTAG

Upstream 100 bases:

>100_bases
CCGGTTCGGCACGAGTAGGGCCGAAGGGCACGGGTGCCCGGGACGCCCGGGCACGCCTCCCGATCCGCGTCCCGGTCGGG
GGCTGGGGGAGGATGGGGCC

Downstream 100 bases:

>100_bases
GCCCCGGGCGGGGCGCCGGGGCGGCCGTCGTCCGACGTACCACCAGCCGGGTCGGCAGCAGCACCTGCTCGGGCTCCCAG
TCGCGCCGGCCGAGCGCGCC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MPEHAPDGVRPARPHHKLTADGRRMREVLTYSRRGSRFSPRQQQAWAAYAERWWVPDEAVDDPGFTLAGCFERSAPLIVE
IGSGIGESTVALAAARPDHDVVAIEVWRPGVADTLGRIGAAGLSNVRLLSVDAVWSMAHLVEPDSLAALWTFFPDPWHKA
KHHKRRLVTASFARLVAGRLAPGAEWRLATDWADYADQMVEVLDAEPMLEGGVVERWAERPVTKFERKGIAAGRAITDLA
YRRRGAAR

Sequences:

>Translated_248_residues
MPEHAPDGVRPARPHHKLTADGRRMREVLTYSRRGSRFSPRQQQAWAAYAERWWVPDEAVDDPGFTLAGCFERSAPLIVE
IGSGIGESTVALAAARPDHDVVAIEVWRPGVADTLGRIGAAGLSNVRLLSVDAVWSMAHLVEPDSLAALWTFFPDPWHKA
KHHKRRLVTASFARLVAGRLAPGAEWRLATDWADYADQMVEVLDAEPMLEGGVVERWAERPVTKFERKGIAAGRAITDLA
YRRRGAAR
>Mature_247_residues
PEHAPDGVRPARPHHKLTADGRRMREVLTYSRRGSRFSPRQQQAWAAYAERWWVPDEAVDDPGFTLAGCFERSAPLIVEI
GSGIGESTVALAAARPDHDVVAIEVWRPGVADTLGRIGAAGLSNVRLLSVDAVWSMAHLVEPDSLAALWTFFPDPWHKAK
HHKRRLVTASFARLVAGRLAPGAEWRLATDWADYADQMVEVLDAEPMLEGGVVERWAERPVTKFERKGIAAGRAITDLAY
RRRGAAR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=232, Percent_Identity=38.3620689655172, Blast_Score=160, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_NOCSJ (A1SNN2)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_925104.1
- ProteinModelPortal:   A1SNN2
- SMR:   A1SNN2
- STRING:   A1SNN2
- GeneID:   4598054
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3919
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   A1SNN2
- BioCyc:   NSP35761:NOCA_3919-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27498; Mature: 27367

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: BINDING 80-80 BINDING 105-105 BINDING 132-132 BINDING 155-155 BINDING 159-159 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEHAPDGVRPARPHHKLTADGRRMREVLTYSRRGSRFSPRQQQAWAAYAERWWVPDEAV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
DDPGFTLAGCFERSAPLIVEIGSGIGESTVALAAARPDHDVVAIEVWRPGVADTLGRIGA
CCCCCEEEHHHCCCCCEEEEECCCCCCHHEEEEECCCCCCEEEEEEECCCHHHHHHHHHC
AGLSNVRLLSVDAVWSMAHLVEPDSLAALWTFFPDPWHKAKHHKRRLVTASFARLVAGRL
CCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
APGAEWRLATDWADYADQMVEVLDAEPMLEGGVVERWAERPVTKFERKGIAAGRAITDLA
CCCCCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHCCCCCCHHHHHHH
YRRRGAAR
HHHCCCCC
>Mature Secondary Structure 
PEHAPDGVRPARPHHKLTADGRRMREVLTYSRRGSRFSPRQQQAWAAYAERWWVPDEAV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
DDPGFTLAGCFERSAPLIVEIGSGIGESTVALAAARPDHDVVAIEVWRPGVADTLGRIGA
CCCCCEEEHHHCCCCCEEEEECCCCCCHHEEEEECCCCCCEEEEEEECCCHHHHHHHHHC
AGLSNVRLLSVDAVWSMAHLVEPDSLAALWTFFPDPWHKAKHHKRRLVTASFARLVAGRL
CCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
APGAEWRLATDWADYADQMVEVLDAEPMLEGGVVERWAERPVTKFERKGIAAGRAITDLA
CCCCCEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHCCCCCCHHHHHHH
YRRRGAAR
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA