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The map label for this gene is ispE

Identifier: 119718075

GI number: 119718075

Start: 4073974

End: 4074867

Strand: Reverse

Name: ispE

Synonym: Noca_3855

Alternate gene names: 119718075

Gene position: 4074867-4073974 (Counterclockwise)

Preceding gene: 119718076

Following gene: 119718074

Centisome position: 81.73

GC content: 76.4

Gene sequence:

>894_bases
GTGCGCGCGCCCGCCAAGGTCAACCTGCACCTCGGGGTCGGCGCGCCCCGCGAGGACGGCTTCCATCCGCTGGTGACGGT
CTACCAGGCGGTCGGGCTCTACGACGACGTCACGGCGCGCGCGGCGCCGGACTGGAGCGTGGGCGTGGGCCTGCCGGACT
GGATGGACGACGACGCCGTACCCCTCAACGGCGCCAACATCGTGGACCGGGCCGCGGACCTGCTCGCGGCCCATCACGGC
GTCGAACGGACCGGGGAGCTGCACATCGCCAAGGCGATCCCGGTCGCGGGCGGCATGGCCGGCGGCTCCGCCGACGCCGC
CGCGGCCCTGGTCGCCCTGGACCGGCTCTGGGGGCTGGACACCTCCGACGACGACCTCCTCGCGCTCGCCGCCCGGCTGG
GCAGCGACGTGCCGTTCGCCCTGCTCGGCGGCACCGCGCTCGGCACCGGCCGGGGCGAGGTGGTCACGCCGGTGCAGGAC
CGCGGCACCTGGTGGTGGGTGGTCGTCCCCTCCGACACCGGGCTGTCGACCCCCGAGGTCTACCGGCACTTCGACCGGAT
GTTCCCCGACGCACCCTCCCAGCCGGTGCCGGCCGACGCGCTGCTCGGCGCGATCGCCGCCGGCGACACCTGGGCGCTCG
CGGACGCCCTGCACAACGACCTCGAGGCGGCCGCGATCGACCTGCGCCCCGAGCTCGGCCGGCTGATCGAGCGCGGCGAG
GAGGCCGGGGCGCTGCGCGGCCTGGTGTCCGGGTCCGGCCCGACCTGCGTCTTCCTGTGCGGCTCCGCCGACCACGCTCG
CTCGCTGGCCGCCGACCTGTCCGGCGCCGGTCATCCCGTCGTCCTCGCCGCCAACGGGCCGGTCGCCGGCGCGCACCTGG
TCTCCTATGCCTAG

Upstream 100 bases:

>100_bases
TGGCCCGCGGGGAGTCGCTGCGCGTGGAGGACTTCGCCCGGCTTGCGGAGCGGCTGCCGTGGGAGGCGCTGCCCTCGAAG
GAGGGGCCGTGGAGGTGACC

Downstream 100 bases:

>100_bases
CCCGGCGGCCAACCTCGTCAACCTCGAACGGGTCTCCAAGGCGTACGGCGTCCGGCCGCTGCTCACCGAGGTCTCGCTCG
GCATCGGCGCCGGCGAGCGG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRAPAKVNLHLGVGAPREDGFHPLVTVYQAVGLYDDVTARAAPDWSVGVGLPDWMDDDAVPLNGANIVDRAADLLAAHHG
VERTGELHIAKAIPVAGGMAGGSADAAAALVALDRLWGLDTSDDDLLALAARLGSDVPFALLGGTALGTGRGEVVTPVQD
RGTWWWVVVPSDTGLSTPEVYRHFDRMFPDAPSQPVPADALLGAIAAGDTWALADALHNDLEAAAIDLRPELGRLIERGE
EAGALRGLVSGSGPTCVFLCGSADHARSLAADLSGAGHPVVLAANGPVAGAHLVSYA

Sequences:

>Translated_297_residues
MRAPAKVNLHLGVGAPREDGFHPLVTVYQAVGLYDDVTARAAPDWSVGVGLPDWMDDDAVPLNGANIVDRAADLLAAHHG
VERTGELHIAKAIPVAGGMAGGSADAAAALVALDRLWGLDTSDDDLLALAARLGSDVPFALLGGTALGTGRGEVVTPVQD
RGTWWWVVVPSDTGLSTPEVYRHFDRMFPDAPSQPVPADALLGAIAAGDTWALADALHNDLEAAAIDLRPELGRLIERGE
EAGALRGLVSGSGPTCVFLCGSADHARSLAADLSGAGHPVVLAANGPVAGAHLVSYA
>Mature_297_residues
MRAPAKVNLHLGVGAPREDGFHPLVTVYQAVGLYDDVTARAAPDWSVGVGLPDWMDDDAVPLNGANIVDRAADLLAAHHG
VERTGELHIAKAIPVAGGMAGGSADAAAALVALDRLWGLDTSDDDLLALAARLGSDVPFALLGGTALGTGRGEVVTPVQD
RGTWWWVVVPSDTGLSTPEVYRHFDRMFPDAPSQPVPADALLGAIAAGDTWALADALHNDLEAAAIDLRPELGRLIERGE
EAGALRGLVSGSGPTCVFLCGSADHARSLAADLSGAGHPVVLAANGPVAGAHLVSYA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=232, Percent_Identity=31.4655172413793, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_NOCSJ (A1SNG8)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_925040.1
- ProteinModelPortal:   A1SNG8
- STRING:   A1SNG8
- GeneID:   4597990
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3855
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK03188
- BioCyc:   NSP35761:NOCA_3855-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30448; Mature: 30448

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: ACT_SITE 6-6 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPAKVNLHLGVGAPREDGFHPLVTVYQAVGLYDDVTARAAPDWSVGVGLPDWMDDDAV
CCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCEECCCCCCCCCCCCC
PLNGANIVDRAADLLAAHHGVERTGELHIAKAIPVAGGMAGGSADAAAALVALDRLWGLD
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
TSDDDLLALAARLGSDVPFALLGGTALGTGRGEVVTPVQDRGTWWWVVVPSDTGLSTPEV
CCCCHHHHHHHHHCCCCCEEEECCCEECCCCCCEECCEECCCCEEEEEEECCCCCCCHHH
YRHFDRMFPDAPSQPVPADALLGAIAAGDTWALADALHNDLEAAAIDLRPELGRLIERGE
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHEEEECCHHHHHHHHCCC
EAGALRGLVSGSGPTCVFLCGSADHARSLAADLSGAGHPVVLAANGPVAGAHLVSYA
HHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHEECC
>Mature Secondary Structure
MRAPAKVNLHLGVGAPREDGFHPLVTVYQAVGLYDDVTARAAPDWSVGVGLPDWMDDDAV
CCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCEECCCCCCCCCCCCC
PLNGANIVDRAADLLAAHHGVERTGELHIAKAIPVAGGMAGGSADAAAALVALDRLWGLD
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCC
TSDDDLLALAARLGSDVPFALLGGTALGTGRGEVVTPVQDRGTWWWVVVPSDTGLSTPEV
CCCCHHHHHHHHHCCCCCEEEECCCEECCCCCCEECCEECCCCEEEEEEECCCCCCCHHH
YRHFDRMFPDAPSQPVPADALLGAIAAGDTWALADALHNDLEAAAIDLRPELGRLIERGE
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHEEEECCHHHHHHHHCCC
EAGALRGLVSGSGPTCVFLCGSADHARSLAADLSGAGHPVVLAANGPVAGAHLVSYA
HHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA