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The map label for this gene is ksgA [H]

Identifier: 119718076

GI number: 119718076

Start: 4074870

End: 4075763

Strand: Reverse

Name: ksgA [H]

Synonym: Noca_3856

Alternate gene names: 119718076

Gene position: 4075763-4074870 (Counterclockwise)

Preceding gene: 119718077

Following gene: 119718075

Centisome position: 81.75

GC content: 75.95

Gene sequence:

>894_bases
ATGTCTGACACCTCCGCCGGCCCCAGGTTGCTGGGGCCGGCGGACGTGCGCCTGCTCGCCGCCGAGCTCGACCTGCGACC
CACCAAGCAGCGCGGTCAGAACTTCGTGATCGACGCGAACACGGTCCGTCGGATCGTCCGGGAGGCCGGCGTCACCGCCG
AGGACGTGGTCCTCGAGGTCGGTCCCGGCCTGGGCTCGCTGACCCTCGCGCTGCTCGCCGTCGCCGGCCGGGTGGTCGCG
GTCGAGGTGGACCCGGTGCTGGCCGGCCGGCTGCCCGCGACGATCGCGGCGTACGCGCCGGCCCAGGCCGACCGGTTCGA
GGTCGTCCTCGGTGATGCGATGCGGATCCAGGAGGTGCCCGGGCCGACGCCGACCGCGCTGGTCGCGAACCTGCCCTACA
ACGTCTCCGTGCCGGTGCTGCTGCACCTGCTCACGCTGCTGCCCTCCCTCGAGCGGGGCCTGGTGATGGTGCAGGCCGAG
GTCGCCGACCGGCTCGCCGCGGCCCCCGGCTCCAAGGTGTACGGCGTCCCCTCGGTCAAGGCGGCCTGGTTCGCCGACGT
CCGCCGCGCCGGTGCCGTCGGGCGCAACGTGTTCTGGCCCGCGCCGAACGTGGACTCCGGCCTGGTGGCCTGGTCCCGGC
GCGAGCCGCCGGCCACCACCGCCACCCGCGAGCAGGTCTTCGCGGTCGTCGACGCGGCGTTCGCCCAGCGCCGCAAGGCC
CTGCGCGGGGTGCTGCGCTCGCTCGCCCCGGCGGAGTCGGTCGACGCGGCGCTGGCCGGCGCGGGCGTGGACCCGCTGGC
CCGCGGGGAGTCGCTGCGCGTGGAGGACTTCGCCCGGCTTGCGGAGCGGCTGCCGTGGGAGGCGCTGCCCTCGAAGGAGG
GGCCGTGGAGGTGA

Upstream 100 bases:

>100_bases
GGCGCTTGGCCGGGCTGCGCCGCGGCGCTCGGGCTGCCCCGCTGACGTCACCGGAGCCCGCCGCGAAGCGCGCGGGGTCG
CGCTGACTACGCTGGCGCCC

Downstream 100 bases:

>100_bases
CCGTGCGCGCGCCCGCCAAGGTCAACCTGCACCTCGGGGTCGGCGCGCCCCGCGAGGACGGCTTCCATCCGCTGGTGACG
GTCTACCAGGCGGTCGGGCT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSDTSAGPRLLGPADVRLLAAELDLRPTKQRGQNFVIDANTVRRIVREAGVTAEDVVLEVGPGLGSLTLALLAVAGRVVA
VEVDPVLAGRLPATIAAYAPAQADRFEVVLGDAMRIQEVPGPTPTALVANLPYNVSVPVLLHLLTLLPSLERGLVMVQAE
VADRLAAAPGSKVYGVPSVKAAWFADVRRAGAVGRNVFWPAPNVDSGLVAWSRREPPATTATREQVFAVVDAAFAQRRKA
LRGVLRSLAPAESVDAALAGAGVDPLARGESLRVEDFARLAERLPWEALPSKEGPWR

Sequences:

>Translated_297_residues
MSDTSAGPRLLGPADVRLLAAELDLRPTKQRGQNFVIDANTVRRIVREAGVTAEDVVLEVGPGLGSLTLALLAVAGRVVA
VEVDPVLAGRLPATIAAYAPAQADRFEVVLGDAMRIQEVPGPTPTALVANLPYNVSVPVLLHLLTLLPSLERGLVMVQAE
VADRLAAAPGSKVYGVPSVKAAWFADVRRAGAVGRNVFWPAPNVDSGLVAWSRREPPATTATREQVFAVVDAAFAQRRKA
LRGVLRSLAPAESVDAALAGAGVDPLARGESLRVEDFARLAERLPWEALPSKEGPWR
>Mature_296_residues
SDTSAGPRLLGPADVRLLAAELDLRPTKQRGQNFVIDANTVRRIVREAGVTAEDVVLEVGPGLGSLTLALLAVAGRVVAV
EVDPVLAGRLPATIAAYAPAQADRFEVVLGDAMRIQEVPGPTPTALVANLPYNVSVPVLLHLLTLLPSLERGLVMVQAEV
ADRLAAAPGSKVYGVPSVKAAWFADVRRAGAVGRNVFWPAPNVDSGLVAWSRREPPATTATREQVFAVVDAAFAQRRKAL
RGVLRSLAPAESVDAALAGAGVDPLARGESLRVEDFARLAERLPWEALPSKEGPWR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=283, Percent_Identity=29.6819787985866, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI156415992, Length=292, Percent_Identity=29.7945205479452, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786236, Length=269, Percent_Identity=32.3420074349442, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25146882, Length=272, Percent_Identity=30.8823529411765, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25141369, Length=289, Percent_Identity=28.3737024221453, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=185, Percent_Identity=34.5945945945946, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21358017, Length=190, Percent_Identity=34.2105263157895, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357273, Length=298, Percent_Identity=30.2013422818792, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31395; Mature: 31263

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSAGPRLLGPADVRLLAAELDLRPTKQRGQNFVIDANTVRRIVREAGVTAEDVVLEV
CCCCCCCCCCCCCHHHEEEEECCCCCCCHHHCCEEEEEHHHHHHHHHHHCCCHHHEEEEE
GPGLGSLTLALLAVAGRVVAVEVDPVLAGRLPATIAAYAPAQADRFEVVLGDAMRIQEVP
CCCHHHHHHHHHHHHCCEEEEEECCHHHCCCCHHHHHHCCCCCCCEEEEECCCHHHHCCC
GPTPTALVANLPYNVSVPVLLHLLTLLPSLERGLVMVQAEVADRLAAAPGSKVYGVPSVK
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCEECCCCCH
AAWFADVRRAGAVGRNVFWPAPNVDSGLVAWSRREPPATTATREQVFAVVDAAFAQRRKA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LRGVLRSLAPAESVDAALAGAGVDPLARGESLRVEDFARLAERLPWEALPSKEGPWR
HHHHHHHHCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
SDTSAGPRLLGPADVRLLAAELDLRPTKQRGQNFVIDANTVRRIVREAGVTAEDVVLEV
CCCCCCCCCCCCHHHEEEEECCCCCCCHHHCCEEEEEHHHHHHHHHHHCCCHHHEEEEE
GPGLGSLTLALLAVAGRVVAVEVDPVLAGRLPATIAAYAPAQADRFEVVLGDAMRIQEVP
CCCHHHHHHHHHHHHCCEEEEEECCHHHCCCCHHHHHHCCCCCCCEEEEECCCHHHHCCC
GPTPTALVANLPYNVSVPVLLHLLTLLPSLERGLVMVQAEVADRLAAAPGSKVYGVPSVK
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCEECCCCCH
AAWFADVRRAGAVGRNVFWPAPNVDSGLVAWSRREPPATTATREQVFAVVDAAFAQRRKA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LRGVLRSLAPAESVDAALAGAGVDPLARGESLRVEDFARLAERLPWEALPSKEGPWR
HHHHHHHHCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]