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The map label for this gene is cheB

Identifier: 119717821

GI number: 119717821

Start: 3816380

End: 3817480

Strand: Reverse

Name: cheB

Synonym: Noca_3599

Alternate gene names: 119717821

Gene position: 3817480-3816380 (Counterclockwise)

Preceding gene: 119717822

Following gene: 119717820

Centisome position: 76.57

GC content: 73.57

Gene sequence:

>1101_bases
ATGCCCGGTATCAGGGTGCTCGTGGTCGACGACTCCGTGGTGATCCGCAAGCTGGTGTCCGACCTGCTCTCCGCCGACCC
GGACATCGAGGTCGTCGGCACGGCCGTGAACGGCCGGGCCGCGCTGCAGAAGGTCGCGCAGCTCAGGCCGGACCTGGTGA
CCATGGATATCGAGATGCCGGAGATGGACGGCATCGAGTCGGTCCGCGCCATCCGCGCGACCGGCAACAAGGTACCGATC
ATCATGTTCAGCACGCTCACCGAGCGGGGCGCGGTCGCGACCCTGGACGCCCTCGGCGCGGGCGCCTCCGACTACGTGCC
CAAGCCGGCGAACGTCGGCAGCGTGGGCCGCTCGATGGAGCAGGTCCGCGAGGCGCTGATCCCGCGGATCAAGTCGCTGG
TGCCCCGACGCGGCATCACCCCGCACCCCGCTGGTGTCACGGTCGCCCCGACCGCCACCGTGCAGCTGCACGCCCCGGCG
ACGCCTCCGCTCGGAGGCCACCGCCTGCTGGTCATCGGCAGCTCGACCGGCGGCCCCGAGGCCCTCAGCGCGCTGCTGCA
CACGCTGCCGCCGCTCTCGGTCCCGGTGGCGGTCGTGCAGCACATGCCGCCCCTGTTCACCCGCCAGTTCGCCGCCCGCC
TGGACCGGCAGCTGCCGTACGACGTCGTCGAGGCGGAGCACAACCAGCCGCTGCGGCCGGGCACCGTGGCGATCGCGCCC
GGCGACTACCACCTCGAGGTGACCACCGACGCCGCCGGCCTCCGCACCCGGCTCACCCAGGCGCCGCCCGAGAACTACTG
CCGGCCGGCCGTCGACGTGCTGTTCCGCAGCGCCGTGGCCGCCGTCGGCCCGGCGGTGCTGGCCGCTGTGCTGACCGGCA
TGGGGTCCGACGGCTGCAAGGGCGCGAGGCTGATCGTCGAGCGCGGCGGCAGCGTCCTGGCCCAGGACCAGGCCACCTCC
GTGGTCTGGGGGATGCCGGGCGCCGTCGCCACCGCGGGGCTGGCCGAACGGGTGCTGCCCCTTCTCGAGCTCGGACCGGA
GATCCTGCGCCGGCTCGCTCGCCAACGTCCGTCCCTCGTCCCGGCAGGAGCGCTGTCATGA

Upstream 100 bases:

>100_bases
CTGACATCCGGACCTGGGTGCGTCGCACTCCTTAGGTATCCGCCCGCGCCGCCGATGGTCACTACGTCCGCAGCCTGGCG
GACAGGACGAGAGGACGCCC

Downstream 100 bases:

>100_bases
GCATCGGGCACGACGCCTTCACGTTCGTCCGCGAGCTGGTGCGAGCGGAGAGCGCGATCGTCCTCGAGCCGGGCAAGGAG
TACCTCGTCGAGTCCCGCCT

Product: response regulator receiver modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MPGIRVLVVDDSVVIRKLVSDLLSADPDIEVVGTAVNGRAALQKVAQLRPDLVTMDIEMPEMDGIESVRAIRATGNKVPI
IMFSTLTERGAVATLDALGAGASDYVPKPANVGSVGRSMEQVREALIPRIKSLVPRRGITPHPAGVTVAPTATVQLHAPA
TPPLGGHRLLVIGSSTGGPEALSALLHTLPPLSVPVAVVQHMPPLFTRQFAARLDRQLPYDVVEAEHNQPLRPGTVAIAP
GDYHLEVTTDAAGLRTRLTQAPPENYCRPAVDVLFRSAVAAVGPAVLAAVLTGMGSDGCKGARLIVERGGSVLAQDQATS
VVWGMPGAVATAGLAERVLPLLELGPEILRRLARQRPSLVPAGALS

Sequences:

>Translated_366_residues
MPGIRVLVVDDSVVIRKLVSDLLSADPDIEVVGTAVNGRAALQKVAQLRPDLVTMDIEMPEMDGIESVRAIRATGNKVPI
IMFSTLTERGAVATLDALGAGASDYVPKPANVGSVGRSMEQVREALIPRIKSLVPRRGITPHPAGVTVAPTATVQLHAPA
TPPLGGHRLLVIGSSTGGPEALSALLHTLPPLSVPVAVVQHMPPLFTRQFAARLDRQLPYDVVEAEHNQPLRPGTVAIAP
GDYHLEVTTDAAGLRTRLTQAPPENYCRPAVDVLFRSAVAAVGPAVLAAVLTGMGSDGCKGARLIVERGGSVLAQDQATS
VVWGMPGAVATAGLAERVLPLLELGPEILRRLARQRPSLVPAGALS
>Mature_365_residues
PGIRVLVVDDSVVIRKLVSDLLSADPDIEVVGTAVNGRAALQKVAQLRPDLVTMDIEMPEMDGIESVRAIRATGNKVPII
MFSTLTERGAVATLDALGAGASDYVPKPANVGSVGRSMEQVREALIPRIKSLVPRRGITPHPAGVTVAPTATVQLHAPAT
PPLGGHRLLVIGSSTGGPEALSALLHTLPPLSVPVAVVQHMPPLFTRQFAARLDRQLPYDVVEAEHNQPLRPGTVAIAPG
DYHLEVTTDAAGLRTRLTQAPPENYCRPAVDVLFRSAVAAVGPAVLAAVLTGMGSDGCKGARLIVERGGSVLAQDQATSV
VWGMPGAVATAGLAERVLPLLELGPEILRRLARQRPSLVPAGALS

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=361, Percent_Identity=34.6260387811634, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788550, Length=112, Percent_Identity=37.5, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082052, Length=132, Percent_Identity=31.0606060606061, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB_NOCSJ (A1SMR4)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924786.1
- ProteinModelPortal:   A1SMR4
- SMR:   A1SMR4
- STRING:   A1SMR4
- GeneID:   4599402
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3599
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   YLFESIS
- PhylomeDB:   A1SMR4
- BioCyc:   NSP35761:NOCA_3599-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38225; Mature: 38094

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 175-175 ACT_SITE 201-201 ACT_SITE 297-297

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIRVLVVDDSVVIRKLVSDLLSADPDIEVVGTAVNGRAALQKVAQLRPDLVTMDIEMP
CCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCC
EMDGIESVRAIRATGNKVPIIMFSTLTERGAVATLDALGAGASDYVPKPANVGSVGRSME
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEHHHHCCCCCCCCCCCCCCCHHHHHHH
QVREALIPRIKSLVPRRGITPHPAGVTVAPTATVQLHAPATPPLGGHRLLVIGSSTGGPE
HHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCEEEEEECCCCCHH
ALSALLHTLPPLSVPVAVVQHMPPLFTRQFAARLDRQLPYDVVEAEHNQPLRPGTVAIAP
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECC
GDYHLEVTTDAAGLRTRLTQAPPENYCRPAVDVLFRSAVAAVGPAVLAAVLTGMGSDGCK
CCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GARLIVERGGSVLAQDQATSVVWGMPGAVATAGLAERVLPLLELGPEILRRLARQRPSLV
CCEEEEECCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
PAGALS
CCCCCC
>Mature Secondary Structure 
PGIRVLVVDDSVVIRKLVSDLLSADPDIEVVGTAVNGRAALQKVAQLRPDLVTMDIEMP
CCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCC
EMDGIESVRAIRATGNKVPIIMFSTLTERGAVATLDALGAGASDYVPKPANVGSVGRSME
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEHHHHCCCCCCCCCCCCCCCHHHHHHH
QVREALIPRIKSLVPRRGITPHPAGVTVAPTATVQLHAPATPPLGGHRLLVIGSSTGGPE
HHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCEEEEEECCCCCHH
ALSALLHTLPPLSVPVAVVQHMPPLFTRQFAARLDRQLPYDVVEAEHNQPLRPGTVAIAP
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECC
GDYHLEVTTDAAGLRTRLTQAPPENYCRPAVDVLFRSAVAAVGPAVLAAVLTGMGSDGCK
CCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GARLIVERGGSVLAQDQATSVVWGMPGAVATAGLAERVLPLLELGPEILRRLARQRPSLV
CCEEEEECCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
PAGALS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA