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The map label for this gene is tcmA [H]

Identifier: 119717724

GI number: 119717724

Start: 3710699

End: 3712123

Strand: Reverse

Name: tcmA [H]

Synonym: Noca_3502

Alternate gene names: 119717724

Gene position: 3712123-3710699 (Counterclockwise)

Preceding gene: 119717727

Following gene: 119717723

Centisome position: 74.45

GC content: 73.68

Gene sequence:

>1425_bases
ATGACAGCTCTCGACGTGGCGGTCGCACCGGCCCCGACGCGGTCCCGACGCTCGCCGGTGTGGCTCGCCGTCCTCGCGGC
CTCGCTGCCGATGTTCATGGCCACCCTTGACAACCTGGTGATGACCAGCGCGCTGCCGGTGATCCGGACCGACCTCGGCT
CGTCGGTCAACCAGCTCTCCTGGTTCATGAACGCCTATACGCTGACCTTCGCGACCTTCATGCTCCCCGCCGCGACCCTC
GGCGACCGGCTCGGCCGACGGCGGATGATGCTCGCGGGCCTGACCGTCTTCACGCTCGCGTCGGTCGCGTCGGCGCTGAG
CACCACCTCGGAGGCGCTGATCGCGGCGCGCGCCGTGCAGGGCCTCGGTGCGGCGGCGATCATGCCGCTCTCCCTGACCC
TGCTCGCCTCCGCCGTGCCCCCGGCCCTGCGCTCGGCCGCCATCGGGATCTGGGGTGGCGTCAGCGGGCTCGGTGTCGCG
CTCGGTCCCGTGGTCGGCGGCGCGGTCGTCGAGGGCGTCAGCTGGCAGGCGATCTTCTGGCTCAACGTCCCGGTGGCCGC
GGTCGCGGGGCCGCTGCTGGTCCTCGGGGTGCGCGAGTCGCACGGCGCCTGGCAGCGGCTCGACCTGGTCGGGACCCTGC
TCGTCGGCGGCGCGGTCCTCCTCGGGATCTGGGGCATCGTGCACGGCAACGACGACGGCTGGGGCGATCCGCGGGTCCTC
GGCCCGCTCGTGGTCGCTGCGCTGCTGGCGCCGGCGTACCTGCGCTGGGCCCGAGGTCGTTCCCACGCGGTCCTGCCGCT
GCGGCTGTTCGCCGCGCGTGGGTTCTCCGTCGCGAACGTGATCGCCCTGTTCTTCACCATCGGGATGTTCGGGACGGTCT
TCCTGCTCACGCAGTACCTCCAGGTGGTCCAGGGCTACAGCCCGCTCGCCGCTGGCGTGCGCACGCTGCCGTGGACGGCC
GCACCGATGGTGGTCGCGCCGCTCGCCGGCCTGCTGGCGCCGCGCACCGGCTTGCGGGCGCTGCTCCTGACGGGGCTGGC
GCTCCAGACCGGGTCGCTGGTCTGGTTCGCGGTGCTCACCGAGACCGCTGCGGGCTACCCGGCGTTCATGCCGGCGCTGC
TGATGGCCGGGGTCGGGATGGGGCTGACGTTCGCACCGATGGCGACTGCCGTGCTCGAGGGCCTGCCCGAGGAGGACTTC
GCCATGGCCAGCTCGGCCAACTCCACGATCCGCGAGTTCGGGGTCGCGCTCGGCATCGCCGTGCTCACGGCGGTCTTCCT
CGGCAACGGCGGCGCGATCGAGCCGCTCGGGTACGACGGCGCGATCGGCCCGGCGCTGCTGACCGGCGCCGGGGCCGTGG
CGGTTGCGACACTCGCCGCGCTGCTCGCTCCCGGCAGGGGGAGGCGGGCCACCCCTCGGGCCTGA

Upstream 100 bases:

>100_bases
TCCGGACCGCCTTCGTGGCACTCATGCTTGACATGTTATCAGTCGATTACTTAGTGTCCTTTCGGTCAAGTAATCATTCA
ATTACAACCAAGGACCTCCG

Downstream 100 bases:

>100_bases
CGGCCCTCGGGCATGATGGCGCGGTGCGCCGCCGCCCCTCGCCGACCGCCTCGTCGATCCGGCCCCCGGTCCTGATGCTG
GTCGCCCTGGTGGCCGCCGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTALDVAVAPAPTRSRRSPVWLAVLAASLPMFMATLDNLVMTSALPVIRTDLGSSVNQLSWFMNAYTLTFATFMLPAATL
GDRLGRRRMMLAGLTVFTLASVASALSTTSEALIAARAVQGLGAAAIMPLSLTLLASAVPPALRSAAIGIWGGVSGLGVA
LGPVVGGAVVEGVSWQAIFWLNVPVAAVAGPLLVLGVRESHGAWQRLDLVGTLLVGGAVLLGIWGIVHGNDDGWGDPRVL
GPLVVAALLAPAYLRWARGRSHAVLPLRLFAARGFSVANVIALFFTIGMFGTVFLLTQYLQVVQGYSPLAAGVRTLPWTA
APMVVAPLAGLLAPRTGLRALLLTGLALQTGSLVWFAVLTETAAGYPAFMPALLMAGVGMGLTFAPMATAVLEGLPEEDF
AMASSANSTIREFGVALGIAVLTAVFLGNGGAIEPLGYDGAIGPALLTGAGAVAVATLAALLAPGRGRRATPRA

Sequences:

>Translated_474_residues
MTALDVAVAPAPTRSRRSPVWLAVLAASLPMFMATLDNLVMTSALPVIRTDLGSSVNQLSWFMNAYTLTFATFMLPAATL
GDRLGRRRMMLAGLTVFTLASVASALSTTSEALIAARAVQGLGAAAIMPLSLTLLASAVPPALRSAAIGIWGGVSGLGVA
LGPVVGGAVVEGVSWQAIFWLNVPVAAVAGPLLVLGVRESHGAWQRLDLVGTLLVGGAVLLGIWGIVHGNDDGWGDPRVL
GPLVVAALLAPAYLRWARGRSHAVLPLRLFAARGFSVANVIALFFTIGMFGTVFLLTQYLQVVQGYSPLAAGVRTLPWTA
APMVVAPLAGLLAPRTGLRALLLTGLALQTGSLVWFAVLTETAAGYPAFMPALLMAGVGMGLTFAPMATAVLEGLPEEDF
AMASSANSTIREFGVALGIAVLTAVFLGNGGAIEPLGYDGAIGPALLTGAGAVAVATLAALLAPGRGRRATPRA
>Mature_473_residues
TALDVAVAPAPTRSRRSPVWLAVLAASLPMFMATLDNLVMTSALPVIRTDLGSSVNQLSWFMNAYTLTFATFMLPAATLG
DRLGRRRMMLAGLTVFTLASVASALSTTSEALIAARAVQGLGAAAIMPLSLTLLASAVPPALRSAAIGIWGGVSGLGVAL
GPVVGGAVVEGVSWQAIFWLNVPVAAVAGPLLVLGVRESHGAWQRLDLVGTLLVGGAVLLGIWGIVHGNDDGWGDPRVLG
PLVVAALLAPAYLRWARGRSHAVLPLRLFAARGFSVANVIALFFTIGMFGTVFLLTQYLQVVQGYSPLAAGVRTLPWTAA
PMVVAPLAGLLAPRTGLRALLLTGLALQTGSLVWFAVLTETAAGYPAFMPALLMAGVGMGLTFAPMATAVLEGLPEEDFA
MASSANSTIREFGVALGIAVLTAVFLGNGGAIEPLGYDGAIGPALLTGAGAVAVATLAALLAPGRGRRATPRA

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=406, Percent_Identity=30.2955665024631, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788710, Length=420, Percent_Identity=28.3333333333333, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081983, Length=430, Percent_Identity=29.3023255813954, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1790195, Length=416, Percent_Identity=30.2884615384615, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788392, Length=419, Percent_Identity=30.0715990453461, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1790146, Length=136, Percent_Identity=31.6176470588235, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=425, Percent_Identity=25.8823529411765, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322958, Length=469, Percent_Identity=21.9616204690832, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319770, Length=384, Percent_Identity=23.6979166666667, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=136, Percent_Identity=31.6176470588235, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=296, Percent_Identity=22.2972972972973, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=24.1830065359477, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48469; Mature: 48338

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALDVAVAPAPTRSRRSPVWLAVLAASLPMFMATLDNLVMTSALPVIRTDLGSSVNQLS
CCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WFMNAYTLTFATFMLPAATLGDRLGRRRMMLAGLTVFTLASVASALSTTSEALIAARAVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGAAAIMPLSLTLLASAVPPALRSAAIGIWGGVSGLGVALGPVVGGAVVEGVSWQAIFW
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
LNVPVAAVAGPLLVLGVRESHGAWQRLDLVGTLLVGGAVLLGIWGIVHGNDDGWGDPRVL
ECCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHH
GPLVVAALLAPAYLRWARGRSHAVLPLRLFAARGFSVANVIALFFTIGMFGTVFLLTQYL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QVVQGYSPLAAGVRTLPWTAAPMVVAPLAGLLAPRTGLRALLLTGLALQTGSLVWFAVLT
HHHCCCCHHHHCHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ETAAGYPAFMPALLMAGVGMGLTFAPMATAVLEGLPEEDFAMASSANSTIREFGVALGIA
HHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHH
VLTAVFLGNGGAIEPLGYDGAIGPALLTGAGAVAVATLAALLAPGRGRRATPRA
HHHHHHHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TALDVAVAPAPTRSRRSPVWLAVLAASLPMFMATLDNLVMTSALPVIRTDLGSSVNQLS
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WFMNAYTLTFATFMLPAATLGDRLGRRRMMLAGLTVFTLASVASALSTTSEALIAARAVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGAAAIMPLSLTLLASAVPPALRSAAIGIWGGVSGLGVALGPVVGGAVVEGVSWQAIFW
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
LNVPVAAVAGPLLVLGVRESHGAWQRLDLVGTLLVGGAVLLGIWGIVHGNDDGWGDPRVL
ECCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHH
GPLVVAALLAPAYLRWARGRSHAVLPLRLFAARGFSVANVIALFFTIGMFGTVFLLTQYL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QVVQGYSPLAAGVRTLPWTAAPMVVAPLAGLLAPRTGLRALLLTGLALQTGSLVWFAVLT
HHHCCCCHHHHCHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
ETAAGYPAFMPALLMAGVGMGLTFAPMATAVLEGLPEEDFAMASSANSTIREFGVALGIA
HHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHH
VLTAVFLGNGGAIEPLGYDGAIGPALLTGAGAVAVATLAALLAPGRGRRATPRA
HHHHHHHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]