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The map label for this gene is birA [H]

Identifier: 119717716

GI number: 119717716

Start: 3702624

End: 3703415

Strand: Reverse

Name: birA [H]

Synonym: Noca_3494

Alternate gene names: 119717716

Gene position: 3703415-3702624 (Counterclockwise)

Preceding gene: 119717722

Following gene: 119717715

Centisome position: 74.28

GC content: 75.0

Gene sequence:

>792_bases
GTGCGACGCACATCACTCGACCCCCGCCCGCTCGCAGCGCTTGCACCCGACCTCGTCGTCGAGGTGCTCGAGAGCGCGAC
CTCGTCCAACGCGGTCGCGTCCGACCGCGCCCGGGAGGGCGCCGACGAGGGACTGGTCATCGTCGCCGAGCACCAGAGCG
CCGGGCGGGGCCGCCTGGACCGGGCCTGGGAGGCCCCCGCCCGGTCCGCGCTCACGTTCTCGTTGCTGGTCCGGCCCACG
GCGCCGGCAGCCGACTGGCCGTGGCTGCCGCTGCTCACCGGCTACACCGTGATGAAGACGCTGCGCGCCGAGGGGTACGA
CGTCGGGGTGAAGTGGCCCAACGACGTGCTGGTCGGCGATCGCAAGCTCGTCGGCATCCTCGTCGAGCGGGTCGAGACCC
CCTCCGGTCCGGCCGCTGTGCTCGGGGTGGGGCTGAACGTCAGCCTCACCGCGGACGAGCTGCCGGTCCCGACGGCGACG
TCGCTGACACTCGAGTCCGGCGCGGAGCCGGACCGGACCCACCTGCTCGTCGAGCTGCTCCGCTCGCTGCGCGAGGCCTA
CGACGTCTGGCAGGCAGGCGGTGCCGAGGCCACCGCCCGGCTGCGCTCGTCGTACGCCGCGGCCTGTGTGACCGTGGGCC
GCGAGGTGCGCGTCGACCTGCCCGGGGGTCAGCTGCTGACCGGCCGGGCGACCGGCGTCGACGGGGCCGGGCGCCTGGTG
GTGCTGGGCGCGGACGGCGAGACCGTCGTCGGCGCGGGTGACGTAGTGCACGTGCGCGCCGCCGATCAGTGA

Upstream 100 bases:

>100_bases
CCGGGCTGGGCGCTCGCCGTCGCTGCCATCTTCTGGGGGGCTCCTCACTGGCACGGGGTTGGGATCGTGGGCCAATGTAG
TGCCCGTGACCTCCGACCCG

Downstream 100 bases:

>100_bases
CATGATCGGCGCGTGGCCATCTCGCACAAGCTGCTGAACGAGGGTGAGACCGTCGTCGTCGACACCCGTACGCACGTCAA
GGCGCTGATCGTCCCGCTCC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRRTSLDPRPLAALAPDLVVEVLESATSSNAVASDRAREGADEGLVIVAEHQSAGRGRLDRAWEAPARSALTFSLLVRPT
APAADWPWLPLLTGYTVMKTLRAEGYDVGVKWPNDVLVGDRKLVGILVERVETPSGPAAVLGVGLNVSLTADELPVPTAT
SLTLESGAEPDRTHLLVELLRSLREAYDVWQAGGAEATARLRSSYAAACVTVGREVRVDLPGGQLLTGRATGVDGAGRLV
VLGADGETVVGAGDVVHVRAADQ

Sequences:

>Translated_263_residues
MRRTSLDPRPLAALAPDLVVEVLESATSSNAVASDRAREGADEGLVIVAEHQSAGRGRLDRAWEAPARSALTFSLLVRPT
APAADWPWLPLLTGYTVMKTLRAEGYDVGVKWPNDVLVGDRKLVGILVERVETPSGPAAVLGVGLNVSLTADELPVPTAT
SLTLESGAEPDRTHLLVELLRSLREAYDVWQAGGAEATARLRSSYAAACVTVGREVRVDLPGGQLLTGRATGVDGAGRLV
VLGADGETVVGAGDVVHVRAADQ
>Mature_263_residues
MRRTSLDPRPLAALAPDLVVEVLESATSSNAVASDRAREGADEGLVIVAEHQSAGRGRLDRAWEAPARSALTFSLLVRPT
APAADWPWLPLLTGYTVMKTLRAEGYDVGVKWPNDVLVGDRKLVGILVERVETPSGPAAVLGVGLNVSLTADELPVPTAT
SLTLESGAEPDRTHLLVELLRSLREAYDVWQAGGAEATARLRSSYAAACVTVGREVRVDLPGGQLLTGRATGVDGAGRLV
VLGADGETVVGAGDVVHVRAADQ

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=247, Percent_Identity=33.6032388663968, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27551; Mature: 27551

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTSLDPRPLAALAPDLVVEVLESATSSNAVASDRAREGADEGLVIVAEHQSAGRGRLD
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCC
RAWEAPARSALTFSLLVRPTAPAADWPWLPLLTGYTVMKTLRAEGYDVGVKWPNDVLVGD
HHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEECC
RKLVGILVERVETPSGPAAVLGVGLNVSLTADELPVPTATSLTLESGAEPDRTHLLVELL
HHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHH
RSLREAYDVWQAGGAEATARLRSSYAAACVTVGREVRVDLPGGQLLTGRATGVDGAGRLV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCCEEE
VLGADGETVVGAGDVVHVRAADQ
EEECCCCEEEECCCEEEEEECCC
>Mature Secondary Structure
MRRTSLDPRPLAALAPDLVVEVLESATSSNAVASDRAREGADEGLVIVAEHQSAGRGRLD
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCC
RAWEAPARSALTFSLLVRPTAPAADWPWLPLLTGYTVMKTLRAEGYDVGVKWPNDVLVGD
HHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEECC
RKLVGILVERVETPSGPAAVLGVGLNVSLTADELPVPTATSLTLESGAEPDRTHLLVELL
HHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHH
RSLREAYDVWQAGGAEATARLRSSYAAACVTVGREVRVDLPGGQLLTGRATGVDGAGRLV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCCEEE
VLGADGETVVGAGDVVHVRAADQ
EEECCCCEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]