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The map label for this gene is gatB

Identifier: 119717657

GI number: 119717657

Start: 3640758

End: 3642251

Strand: Reverse

Name: gatB

Synonym: Noca_3435

Alternate gene names: 119717657

Gene position: 3642251-3640758 (Counterclockwise)

Preceding gene: 119717658

Following gene: 119717656

Centisome position: 73.05

GC content: 71.22

Gene sequence:

>1494_bases
GTGACCGAGACCCTCGTCGCCTTCGACGACGTCCTGGCCTCCTACGACCCGGCGATGGGCCTGGAGGTCCACGTCGAGCT
GAACACCGCCTCGAAGATGTTCTGCGGCTGCCCGACCGAGTTCGGCGCCGAGCCCAACACCCAGGTCTGCCCGACCTGCC
TGGGCCTGCCCGGGGCGATGCCGGTCGTCAACGGCAAGGCGGTCGAGTCGGCGATCCGGATCGGGCTGGCGCTGAACTGC
GAGATCGCCGAGTGGTGCCGGTTCGCGCGGAAGAACTACTTCTACCCGGACATGCCGAAGAACTTCCAGACCTCGCAGTA
CGACGAGCCGATCTGCTTCGAGGGCTACATGGACGTCGATGTGGACGGCGAGACGTTCCGGGTCGAGATCGAGCGGGCGC
ACATGGAGGAGGACACCGGCAAGTCGCTGCACGTCGGTGGCGCGACCGGCCGCATCCACGGCGCCGACCACTCGCTGGTC
GACTACAACCGGGCCGGCATCCCGCTCATCGAGATCGTCACCAAGCCGATCGTCGGCGCCGGCGCGAAGGCGCCGGAGGT
CGCCCGCGCGTACGTCGCGCAGCTGCGCGACCTGATCATCGCGCTCGGGGTCTCCGACGCGCGGATGGACCAGGGGTCGA
TCCGCGCCGACGTGAACCTGTCGCTCTCACCCAAGGACTCGGGGACGCTCGGGACCCGCACCGAGACCAAGAACGTCAAC
TCGCTGCGCTCGGTCGAGCGCGCGGTCCGCTACGAGATGTCCCGCCACGCCGGCGTCCTGGACGCCGGGCGGCCGATCCT
CCAGGAGACCCGGCACTGGCACGAGGACACCGGCGTCACCACGAGCGGCCGGGAGAAGTCCGACGCGGAGGACTACCGCT
ACTTCCCCGAGCCCGACCTGGTGCCGGTCGCTCCGACCCGCGCCTGGGTGGAGGAGTTGCGCGGCACGCTGCCCGAGAAC
CCGACCCAGAAGCGCGCCCGGCTGCAGGCGGAGTGGGGCTTCTCCGACCTCGAGATGCGCGACACCGTCGGTGCGGGTGC
GCTGCTGATCGTCGAGGAGACGATCGCGGCGGGCGCCTCGCCCCAGGCCGCCCGCAAGTGGTGGCTGGGCGAGATGGCCC
GCCGCGCCAACGAGGCCGGTGTCGAGGTCGGCGAGCTCGGGGTCACCCCGGTCGACGTCGCGCGGGTCCAGGTGCTGGTC
GACGAGGGGAAGCTCAACGACAAGCTGGCGCGCCAGGTCTTCGACGGGCTGCTCGCCGGCGAGGGCAGCCCCGACGAGAT
CGTGGCGGCCCGCGGCCTGGCGATCGTCTCCGACGAGGGCGCCCTGTCCGCGGCGGTGGACAGCGCGATCGCTGCGAACC
CGGACGTCGCGGACAAGATCCGCGACGGCAAGGTCGCCGCCGCCGGTGCCCTGATCGGCGCGGTGATGAAGGAGATGCGC
GGCCAGGCCGATGCCGCCCGGGTCCGCGAGCTGATCCTGGAGAAGCTCGCCTAG

Upstream 100 bases:

>100_bases
ACCGCTGCTACCGCGTCGGCGCCGCCCTCGAGGCGCTGCTCCACGACCAGTGGGGTGGACCCCTGCTCGACAAGGCCCCC
GCTCTGGAAGGAGCACGCCC

Downstream 100 bases:

>100_bases
CCGGGTCGAGTCGGCGCGTCTTGCTCGCTCAGGAGGGTCGAGTCGGCGTGTCTTGCTCGCTCAGGAGGGTCGAGTCGGCG
CGTCTTGCTCGCTTCGCCCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTETLVAFDDVLASYDPAMGLEVHVELNTASKMFCGCPTEFGAEPNTQVCPTCLGLPGAMPVVNGKAVESAIRIGLALNC
EIAEWCRFARKNYFYPDMPKNFQTSQYDEPICFEGYMDVDVDGETFRVEIERAHMEEDTGKSLHVGGATGRIHGADHSLV
DYNRAGIPLIEIVTKPIVGAGAKAPEVARAYVAQLRDLIIALGVSDARMDQGSIRADVNLSLSPKDSGTLGTRTETKNVN
SLRSVERAVRYEMSRHAGVLDAGRPILQETRHWHEDTGVTTSGREKSDAEDYRYFPEPDLVPVAPTRAWVEELRGTLPEN
PTQKRARLQAEWGFSDLEMRDTVGAGALLIVEETIAAGASPQAARKWWLGEMARRANEAGVEVGELGVTPVDVARVQVLV
DEGKLNDKLARQVFDGLLAGEGSPDEIVAARGLAIVSDEGALSAAVDSAIAANPDVADKIRDGKVAAAGALIGAVMKEMR
GQADAARVRELILEKLA

Sequences:

>Translated_497_residues
MTETLVAFDDVLASYDPAMGLEVHVELNTASKMFCGCPTEFGAEPNTQVCPTCLGLPGAMPVVNGKAVESAIRIGLALNC
EIAEWCRFARKNYFYPDMPKNFQTSQYDEPICFEGYMDVDVDGETFRVEIERAHMEEDTGKSLHVGGATGRIHGADHSLV
DYNRAGIPLIEIVTKPIVGAGAKAPEVARAYVAQLRDLIIALGVSDARMDQGSIRADVNLSLSPKDSGTLGTRTETKNVN
SLRSVERAVRYEMSRHAGVLDAGRPILQETRHWHEDTGVTTSGREKSDAEDYRYFPEPDLVPVAPTRAWVEELRGTLPEN
PTQKRARLQAEWGFSDLEMRDTVGAGALLIVEETIAAGASPQAARKWWLGEMARRANEAGVEVGELGVTPVDVARVQVLV
DEGKLNDKLARQVFDGLLAGEGSPDEIVAARGLAIVSDEGALSAAVDSAIAANPDVADKIRDGKVAAAGALIGAVMKEMR
GQADAARVRELILEKLA
>Mature_496_residues
TETLVAFDDVLASYDPAMGLEVHVELNTASKMFCGCPTEFGAEPNTQVCPTCLGLPGAMPVVNGKAVESAIRIGLALNCE
IAEWCRFARKNYFYPDMPKNFQTSQYDEPICFEGYMDVDVDGETFRVEIERAHMEEDTGKSLHVGGATGRIHGADHSLVD
YNRAGIPLIEIVTKPIVGAGAKAPEVARAYVAQLRDLIIALGVSDARMDQGSIRADVNLSLSPKDSGTLGTRTETKNVNS
LRSVERAVRYEMSRHAGVLDAGRPILQETRHWHEDTGVTTSGREKSDAEDYRYFPEPDLVPVAPTRAWVEELRGTLPENP
TQKRARLQAEWGFSDLEMRDTVGAGALLIVEETIAAGASPQAARKWWLGEMARRANEAGVEVGELGVTPVDVARVQVLVD
EGKLNDKLARQVFDGLLAGEGSPDEIVAARGLAIVSDEGALSAAVDSAIAANPDVADKIRDGKVAAAGALIGAVMKEMRG
QADAARVRELILEKLA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=505, Percent_Identity=33.2673267326733, Blast_Score=267, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI115534094, Length=310, Percent_Identity=36.4516129032258, Blast_Score=196, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319391, Length=314, Percent_Identity=31.8471337579618, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI45550808, Length=509, Percent_Identity=29.4695481335953, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_NOCSJ (A1SMA0)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924622.1
- ProteinModelPortal:   A1SMA0
- SMR:   A1SMA0
- STRING:   A1SMA0
- GeneID:   4597251
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3435
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   NSP35761:NOCA_3435-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53595; Mature: 53464

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLVAFDDVLASYDPAMGLEVHVELNTASKMFCGCPTEFGAEPNTQVCPTCLGLPGAM
CCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEECCCHHHCCCCCCCCCHHHHCCCCCC
PVVNGKAVESAIRIGLALNCEIAEWCRFARKNYFYPDMPKNFQTSQYDEPICFEGYMDVD
CCCCCHHHHHHHHHCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCEEEEE
VDGETFRVEIERAHMEEDTGKSLHVGGATGRIHGADHSLVDYNRAGIPLIEIVTKPIVGA
CCCCEEEEEEEHHHCCCCCCCEEEECCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHCC
GAKAPEVARAYVAQLRDLIIALGVSDARMDQGSIRADVNLSLSPKDSGTLGTRTETKNVN
CCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHH
SLRSVERAVRYEMSRHAGVLDAGRPILQETRHWHEDTGVTTSGREKSDAEDYRYFPEPDL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCC
VPVAPTRAWVEELRGTLPENPTQKRARLQAEWGFSDLEMRDTVGAGALLIVEETIAAGAS
EECCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCEEEEEEHHHHCCCC
PQAARKWWLGEMARRANEAGVEVGELGVTPVDVARVQVLVDEGKLNDKLARQVFDGLLAG
HHHHHHHHHHHHHHHHHHCCCEEHHCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHCC
EGSPDEIVAARGLAIVSDEGALSAAVDSAIAANPDVADKIRDGKVAAAGALIGAVMKEMR
CCCCHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHC
GQADAARVRELILEKLA
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TETLVAFDDVLASYDPAMGLEVHVELNTASKMFCGCPTEFGAEPNTQVCPTCLGLPGAM
CCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEECCCHHHCCCCCCCCCHHHHCCCCCC
PVVNGKAVESAIRIGLALNCEIAEWCRFARKNYFYPDMPKNFQTSQYDEPICFEGYMDVD
CCCCCHHHHHHHHHCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCEEEEE
VDGETFRVEIERAHMEEDTGKSLHVGGATGRIHGADHSLVDYNRAGIPLIEIVTKPIVGA
CCCCEEEEEEEHHHCCCCCCCEEEECCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHCC
GAKAPEVARAYVAQLRDLIIALGVSDARMDQGSIRADVNLSLSPKDSGTLGTRTETKNVN
CCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHH
SLRSVERAVRYEMSRHAGVLDAGRPILQETRHWHEDTGVTTSGREKSDAEDYRYFPEPDL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCC
VPVAPTRAWVEELRGTLPENPTQKRARLQAEWGFSDLEMRDTVGAGALLIVEETIAAGAS
EECCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCEEEEEEHHHHCCCC
PQAARKWWLGEMARRANEAGVEVGELGVTPVDVARVQVLVDEGKLNDKLARQVFDGLLAG
HHHHHHHHHHHHHHHHHHCCCEEHHCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHCC
EGSPDEIVAARGLAIVSDEGALSAAVDSAIAANPDVADKIRDGKVAAAGALIGAVMKEMR
CCCCHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHC
GQADAARVRELILEKLA
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA