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The map label for this gene is bioB

Identifier: 119717637

GI number: 119717637

Start: 3616735

End: 3617859

Strand: Reverse

Name: bioB

Synonym: Noca_3415

Alternate gene names: 119717637

Gene position: 3617859-3616735 (Counterclockwise)

Preceding gene: 119717640

Following gene: 119717636

Centisome position: 72.56

GC content: 69.16

Gene sequence:

>1125_bases
ATGCAGACATCCTTCGATCACCTGGCGGACCGCATCCTTGCCGGAGGTGACGCGACGCCCGCCGACGCGTTGGCGGTGCT
ACGCGCCGACGAGAAGGACCTGCTCCACGTGGTTGCGGCAGCGGGTCGGCTGCGCCGCGCGCGCTTCGGCAACACGGTGA
AGGTCAACTACCTGGTGAACCTGAAGTCCGGGCTCTGTCCGGAGGACTGCCATTACTGCAGCCAGGCGCTGGGATCCCGG
GCGCCGATCCTCAAGTACAACTGGCTCTCGTCCGAGGAGGTCCTGGAGCAGGCCGGTGCCGGCCTGCGAGGCGGGGCGAC
GCGGGTGTGCCTGGTGTCCTCGGGCCGTGGCCCGTCGGACCGGGACGTGGACCGGGTCGCAGCGATGGCCCAGGAACTGA
AGGGTGAGCAGCCCGGCGTCGAGATCTGCGCCTGTCTAGGGTTGCTGAAGGACGGGCAGGCCGAGCGGCTCCGGGCAGCC
GGAGTGGACGCCTACAACCACAACATCAACACCGCCGAATCCCACCACGACACCATTGTCTCGACCCACTCCTACTCCGA
TCGAGTGGACACCATCGAGAAGGCGGCGGCCGCTGGGCTCTCGCCGTGCTCGGGATTGATCGCCGGACTCGGCGAGACCG
ACGAGCAGCTGGTCGAGGCGCTGTTCGCGCTCAAGGCTCTGGGCGCGGACTCGATCCCGGTGAACTTCCTGATGCCGTTC
GACGGCACCCCCAGCGAGCGCACTTTCGAGCTCACGCCGATCCGGTGCGTGCAGATCCTGGCGATGACACGATTCGTGTG
TCCCGATACCGAGATCCGCATCGCCGGCGGCCGCGAGATGCACCTGCGGTCGCTGCAGGCCCTCGCCCTGCATGTCGCGA
ACTCCATCTTCCTCGGCGACTACCTCACTTCCGAGGGCCAGGACGCGCGCGCCGACCTGGAGATGCTGCGCGACAACGGG
TTCGCCATCCTCGGCGCGGAGGCCAAACCCGCCGGCACGGCCACTGCGGCCCACCGCGCCCAGACAGCCCACGACATTGC
CGGCGGCACCTCCGTTGCGGGGTCCGCCCCGGATCCGGCGATCCGCCGCCGTGGCGCCGGAACCGACGTGCCGGCCAACG
CGTGA

Upstream 100 bases:

>100_bases
CTAACCTGAACGCTGTTCACCTGAACGCTGTTCACCTGAACGCTGTTCACCTGAACGCTGTTCACCTGAACGCTGTTCAG
GATCTCGTCGAGGAGGCTGG

Downstream 100 bases:

>100_bases
GCACCGCCCTCGTCGCCGAACCGGCCGAGCGGACCACGCTCGCAGGGCTGCTGGCGTTCGACCGGGCGCACGTGTGGCAT
CCGTACGCCTCGATGACGGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MQTSFDHLADRILAGGDATPADALAVLRADEKDLLHVVAAAGRLRRARFGNTVKVNYLVNLKSGLCPEDCHYCSQALGSR
APILKYNWLSSEEVLEQAGAGLRGGATRVCLVSSGRGPSDRDVDRVAAMAQELKGEQPGVEICACLGLLKDGQAERLRAA
GVDAYNHNINTAESHHDTIVSTHSYSDRVDTIEKAAAAGLSPCSGLIAGLGETDEQLVEALFALKALGADSIPVNFLMPF
DGTPSERTFELTPIRCVQILAMTRFVCPDTEIRIAGGREMHLRSLQALALHVANSIFLGDYLTSEGQDARADLEMLRDNG
FAILGAEAKPAGTATAAHRAQTAHDIAGGTSVAGSAPDPAIRRRGAGTDVPANA

Sequences:

>Translated_374_residues
MQTSFDHLADRILAGGDATPADALAVLRADEKDLLHVVAAAGRLRRARFGNTVKVNYLVNLKSGLCPEDCHYCSQALGSR
APILKYNWLSSEEVLEQAGAGLRGGATRVCLVSSGRGPSDRDVDRVAAMAQELKGEQPGVEICACLGLLKDGQAERLRAA
GVDAYNHNINTAESHHDTIVSTHSYSDRVDTIEKAAAAGLSPCSGLIAGLGETDEQLVEALFALKALGADSIPVNFLMPF
DGTPSERTFELTPIRCVQILAMTRFVCPDTEIRIAGGREMHLRSLQALALHVANSIFLGDYLTSEGQDARADLEMLRDNG
FAILGAEAKPAGTATAAHRAQTAHDIAGGTSVAGSAPDPAIRRRGAGTDVPANA
>Mature_374_residues
MQTSFDHLADRILAGGDATPADALAVLRADEKDLLHVVAAAGRLRRARFGNTVKVNYLVNLKSGLCPEDCHYCSQALGSR
APILKYNWLSSEEVLEQAGAGLRGGATRVCLVSSGRGPSDRDVDRVAAMAQELKGEQPGVEICACLGLLKDGQAERLRAA
GVDAYNHNINTAESHHDTIVSTHSYSDRVDTIEKAAAAGLSPCSGLIAGLGETDEQLVEALFALKALGADSIPVNFLMPF
DGTPSERTFELTPIRCVQILAMTRFVCPDTEIRIAGGREMHLRSLQALALHVANSIFLGDYLTSEGQDARADLEMLRDNG
FAILGAEAKPAGTATAAHRAQTAHDIAGGTSVAGSAPDPAIRRRGAGTDVPANA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=296, Percent_Identity=34.7972972972973, Blast_Score=181, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321725, Length=288, Percent_Identity=30.2083333333333, Blast_Score=148, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_NOCSJ (A1SM80)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924602.1
- ProteinModelPortal:   A1SM80
- SMR:   A1SM80
- STRING:   A1SM80
- GeneID:   4598213
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3415
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A1SM80
- ProtClustDB:   PRK06256
- BioCyc:   NSP35761:NOCA_3415-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39432; Mature: 39432

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSFDHLADRILAGGDATPADALAVLRADEKDLLHVVAAAGRLRRARFGNTVKVNYLVN
CCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
LKSGLCPEDCHYCSQALGSRAPILKYNWLSSEEVLEQAGAGLRGGATRVCLVSSGRGPSD
CCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
RDVDRVAAMAQELKGEQPGVEICACLGLLKDGQAERLRAAGVDAYNHNINTAESHHDTIV
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHCCCCCCCCCCCCCEE
STHSYSDRVDTIEKAAAAGLSPCSGLIAGLGETDEQLVEALFALKALGADSIPVNFLMPF
ECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
DGTPSERTFELTPIRCVQILAMTRFVCPDTEIRIAGGREMHLRSLQALALHVANSIFLGD
CCCCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
YLTSEGQDARADLEMLRDNGFAILGAEAKPAGTATAAHRAQTAHDIAGGTSVAGSAPDPA
HHCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHH
IRRRGAGTDVPANA
HHHCCCCCCCCCCC
>Mature Secondary Structure
MQTSFDHLADRILAGGDATPADALAVLRADEKDLLHVVAAAGRLRRARFGNTVKVNYLVN
CCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
LKSGLCPEDCHYCSQALGSRAPILKYNWLSSEEVLEQAGAGLRGGATRVCLVSSGRGPSD
CCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
RDVDRVAAMAQELKGEQPGVEICACLGLLKDGQAERLRAAGVDAYNHNINTAESHHDTIV
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHCCCCCCCCCCCCCEE
STHSYSDRVDTIEKAAAAGLSPCSGLIAGLGETDEQLVEALFALKALGADSIPVNFLMPF
ECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
DGTPSERTFELTPIRCVQILAMTRFVCPDTEIRIAGGREMHLRSLQALALHVANSIFLGD
CCCCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
YLTSEGQDARADLEMLRDNGFAILGAEAKPAGTATAAHRAQTAHDIAGGTSVAGSAPDPA
HHCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHH
IRRRGAGTDVPANA
HHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA