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The map label for this gene is thiE [H]

Identifier: 119717628

GI number: 119717628

Start: 3608517

End: 3609128

Strand: Reverse

Name: thiE [H]

Synonym: Noca_3406

Alternate gene names: 119717628

Gene position: 3609128-3608517 (Counterclockwise)

Preceding gene: 119717629

Following gene: 119717627

Centisome position: 72.39

GC content: 70.1

Gene sequence:

>612_bases
GTGTTACCCCGCATTCATTGCATCACCGACTTCTCGTCGTACGGCGACAGGGCGATCACCCTGCTGGAGGCCGTCGTCCG
TGAGGGCGTCGACGCCGTCCAGGTGCGTGCGAAGTCGGTGTCCGACCGAGAGGTGGTTGCGTTCACCCGCGCCCTCGTGG
ACCGCCTTGCGGGGACCTCGGCGGCGGTGATCGTGAACGACCGCGTCGACATCGCGCTCATTGCGGGAGCGCAAGGCGTC
CATCTCGGCCGTGATGACCTCGCGGTCGCCGAGGCCAGACGCCTGGCACCGCAAGGCTTCCTCGTCGGCGGCACCTGCCG
GAACGCCGACCAGGCCCGTGAGGCGAGGGCCCAGGGCGCCGACTACATAGGCGTTGGACCGGTCTACGCCACGACCACGA
AGAGTGGTCTCCCGGACCCGATCGGGTTGGACGCGCTGCGTGATGCGGCGCGAGTCCTGCCGGCGATCGCGATCTCTGGC
ATCAACGCCGAGCGCGCGCCCGAGGTGATGGCGGCCGGGGCCTACGGGATCGCGGTGGCCTCGGCCATCTGCCGATCACC
TCAGCCCGGACTGGCCGCGAGGGAACTCGTCGGCGCCGTCGCGCTCGCATGA

Upstream 100 bases:

>100_bases
TCCACACGGGAGCCCGAGGCATCGGGCTGAGATGGGGCTGGAGCCGCTCCGACCGTCGAACCTGATCCGGGTAATGCCGG
CGAAGGAAGTGAGGAGCGAC

Downstream 100 bases:

>100_bases
GGGTGGCGGTGATCGGGGCCGGCATCGTCGGTCTGGCCTGCGCGGAGGAACTGGTCCGCGCGAAGCACGACGTGCGGGTG
TTCGACCCCGACCCGGCTGG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLPRIHCITDFSSYGDRAITLLEAVVREGVDAVQVRAKSVSDREVVAFTRALVDRLAGTSAAVIVNDRVDIALIAGAQGV
HLGRDDLAVAEARRLAPQGFLVGGTCRNADQAREARAQGADYIGVGPVYATTTKSGLPDPIGLDALRDAARVLPAIAISG
INAERAPEVMAAGAYGIAVASAICRSPQPGLAARELVGAVALA

Sequences:

>Translated_203_residues
MLPRIHCITDFSSYGDRAITLLEAVVREGVDAVQVRAKSVSDREVVAFTRALVDRLAGTSAAVIVNDRVDIALIAGAQGV
HLGRDDLAVAEARRLAPQGFLVGGTCRNADQAREARAQGADYIGVGPVYATTTKSGLPDPIGLDALRDAARVLPAIAISG
INAERAPEVMAAGAYGIAVASAICRSPQPGLAARELVGAVALA
>Mature_203_residues
MLPRIHCITDFSSYGDRAITLLEAVVREGVDAVQVRAKSVSDREVVAFTRALVDRLAGTSAAVIVNDRVDIALIAGAQGV
HLGRDDLAVAEARRLAPQGFLVGGTCRNADQAREARAQGADYIGVGPVYATTTKSGLPDPIGLDALRDAARVLPAIAISG
INAERAPEVMAAGAYGIAVASAICRSPQPGLAARELVGAVALA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=183, Percent_Identity=32.7868852459016, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 20907; Mature: 20907

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPRIHCITDFSSYGDRAITLLEAVVREGVDAVQVRAKSVSDREVVAFTRALVDRLAGTS
CCCCEEEECCHHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
AAVIVNDRVDIALIAGAQGVHLGRDDLAVAEARRLAPQGFLVGGTCRNADQAREARAQGA
EEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCC
DYIGVGPVYATTTKSGLPDPIGLDALRDAARVLPAIAISGINAERAPEVMAAGAYGIAVA
CEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCHHHHHHH
SAICRSPQPGLAARELVGAVALA
HHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLPRIHCITDFSSYGDRAITLLEAVVREGVDAVQVRAKSVSDREVVAFTRALVDRLAGTS
CCCCEEEECCHHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
AAVIVNDRVDIALIAGAQGVHLGRDDLAVAEARRLAPQGFLVGGTCRNADQAREARAQGA
EEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCC
DYIGVGPVYATTTKSGLPDPIGLDALRDAARVLPAIAISGINAERAPEVMAAGAYGIAVA
CEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCHHHHCCHHHHHHH
SAICRSPQPGLAARELVGAVALA
HHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA