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The map label for this gene is ilvB [H]

Identifier: 119717530

GI number: 119717530

Start: 3512158

End: 3513924

Strand: Reverse

Name: ilvB [H]

Synonym: Noca_3306

Alternate gene names: 119717530

Gene position: 3513924-3512158 (Counterclockwise)

Preceding gene: 119717532

Following gene: 119717529

Centisome position: 70.48

GC content: 73.46

Gene sequence:

>1767_bases
ATGGCCAAGGGCCTGACCGGTGGTCAGCTGATCGCCAAGATGCTGGCGGCCGAGGCGGTCGACCAGGTGTTCGGGATCAT
CGACGGCACGTACTTCGGGCTCTACCGGGCGCTGGGCGACGAGGGCATCACGCTGCACTCCCCGCGCCACGAGGCGGCCG
CGGCGCACATGGCGGGCGCCTACGCCCGGGTCAGCGGACGGCTCGGGGTCTGCATGGCCTCCAACGGCCCCGGCGTCGCG
AACGTGCTGCCCGGCGTCGCCGTCGAGCAGGGCGAGGGCAACCGGATCCTGCTGATCACCAGCGGCCGGCGGGTCGGCAT
CACCCACCCCGACCGCGGCGGCACCTACCAGAACTTCGACCAGACCGCCGTGACGGCGCCGATGGCCAAGTGGTCCTGCC
ACGTGCCGAGCGCCGACCGACTGCCCGAGATCCTGCGGCGGGCGCTGCGGGTGAGCTTCAGCGGCCGGCCGGGCGTGGTG
CACGTCGACGTCCCCGAGAACATCGTCAACCAGCCGACCGGCCTCGACGCCGGGGCGGTGCGGGCGCCGGAGTCCTACCG
GCGTACGACGCCGCTGCAGCCCGACCCGGTCCTCGTCGAGAAGGCCGCGCAGCTGCTCGTCGAGGCCGAGTGGCCGATGA
TCCACGCCGGCTCCGGGGTCTACCACGCCGGCGCGGAAGCCGAGCTCGCGCGGCTGGCCGGCCTGCTCGCCGCACCGGTG
ACCACCAGCTGGGCGGCCCGCGGCGCACTGTCGGAGGCCCGGCCCGAGGCCATCCCGATGACGGCACTGGGCGTCAACGA
CGAGGTCCGCAACGCCGCCGACGTGGCACTGGTCGTCGGCAGCCGGCTGGGGGAGACCGACTGGTGGGGCAAGGCGCCGA
ACTGGGCGCCGCCGGCGCTGCAGAGGACGATCCAGGTCGACGTCGACGACGAGCGGCTGGGTGTCAACAAGCCGGTCAGC
CTGGCGATCCTGGCCGACGCCCGCGAGGCGCTCCGGGCCCTGGCCGACGCGGTGGAGCGGCGGACGGTCCCGGGGCTGGC
CGCGCGCCGGGACCGGCTCGAGGTGTGGCGCGGCAAGGTCGCCGACGAGCGGGCGAAGCTCGCCAAGGCGGTCAAGCCCG
GGAGCCCGGTCCACCCCGGTCACGTGCCGTCGGTCGCGCAGCAGGTGATGCCCGACGACACCGTGTGGGTCTTCGACGGC
GGCAACACGGTCGTGTGGTCGAACTTCCACCACTCGGCCGACGTGCCGCGGACCATCCTGTCGACGTACAAGTTCGGCAT
GCTCGGCGCCGGCATGGGCCAGGCCCTCGGCGCCGCGGTCGCGGCGCCCGACCAGCGGGTCTGCGTGCTCATCGGCGACG
GTGCGTTCGGCATGCACCCGACCGAGGTCGAGAGCGCGGTGCGCCTCGGGCTGCCCATCGTCTACGTGGTCCTGGTCGAC
GGCGCGTGGGGCATGGTCAAGATGAGCCAGCAGATCCAGGCCCGCCCGATCGCGACGGTGGCGCACAAGATGCTGACCAA
CACCACGCTGCCGGCGGACCAGATCGTGTACGCCGACTTCGCGCCCTGCCGCTACGACCTGATGGGCGAGGCGATGGGAG
CCCACGGCGAGCTGGTGACCGACTCGGCCGACCTGCCGGCCGCGCTGGAGCGGGCCCGCGACTGCGGCCGCGCCGCCGTG
GTGCAGGTGCACGTCGACCAGGTCGAGCACCTCTGGGCGCCGGGCCTGCAAGCGTTCAAGAAGATGCACCAGGAGCCGAA
GGGCTGA

Upstream 100 bases:

>100_bases
TGTGGCGCGTCACAACGGAAGGGGACTTCTGAGGCGGAGGAACAGTACGGCAGCCGTGAGCGACCCCTATCTTGTGGGCA
CAGCCACAAGAGGAGGAGTC

Downstream 100 bases:

>100_bases
CCCGCGATGACCGTCCTCGTGACCGGTGGCACCGGCTACCTCGGCTCCCTCGTCGTGCGCCGGCTGGCCGACCGGGGGAT
CGGGACCGTGTCGGTCGACG

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MAKGLTGGQLIAKMLAAEAVDQVFGIIDGTYFGLYRALGDEGITLHSPRHEAAAAHMAGAYARVSGRLGVCMASNGPGVA
NVLPGVAVEQGEGNRILLITSGRRVGITHPDRGGTYQNFDQTAVTAPMAKWSCHVPSADRLPEILRRALRVSFSGRPGVV
HVDVPENIVNQPTGLDAGAVRAPESYRRTTPLQPDPVLVEKAAQLLVEAEWPMIHAGSGVYHAGAEAELARLAGLLAAPV
TTSWAARGALSEARPEAIPMTALGVNDEVRNAADVALVVGSRLGETDWWGKAPNWAPPALQRTIQVDVDDERLGVNKPVS
LAILADAREALRALADAVERRTVPGLAARRDRLEVWRGKVADERAKLAKAVKPGSPVHPGHVPSVAQQVMPDDTVWVFDG
GNTVVWSNFHHSADVPRTILSTYKFGMLGAGMGQALGAAVAAPDQRVCVLIGDGAFGMHPTEVESAVRLGLPIVYVVLVD
GAWGMVKMSQQIQARPIATVAHKMLTNTTLPADQIVYADFAPCRYDLMGEAMGAHGELVTDSADLPAALERARDCGRAAV
VQVHVDQVEHLWAPGLQAFKKMHQEPKG

Sequences:

>Translated_588_residues
MAKGLTGGQLIAKMLAAEAVDQVFGIIDGTYFGLYRALGDEGITLHSPRHEAAAAHMAGAYARVSGRLGVCMASNGPGVA
NVLPGVAVEQGEGNRILLITSGRRVGITHPDRGGTYQNFDQTAVTAPMAKWSCHVPSADRLPEILRRALRVSFSGRPGVV
HVDVPENIVNQPTGLDAGAVRAPESYRRTTPLQPDPVLVEKAAQLLVEAEWPMIHAGSGVYHAGAEAELARLAGLLAAPV
TTSWAARGALSEARPEAIPMTALGVNDEVRNAADVALVVGSRLGETDWWGKAPNWAPPALQRTIQVDVDDERLGVNKPVS
LAILADAREALRALADAVERRTVPGLAARRDRLEVWRGKVADERAKLAKAVKPGSPVHPGHVPSVAQQVMPDDTVWVFDG
GNTVVWSNFHHSADVPRTILSTYKFGMLGAGMGQALGAAVAAPDQRVCVLIGDGAFGMHPTEVESAVRLGLPIVYVVLVD
GAWGMVKMSQQIQARPIATVAHKMLTNTTLPADQIVYADFAPCRYDLMGEAMGAHGELVTDSADLPAALERARDCGRAAV
VQVHVDQVEHLWAPGLQAFKKMHQEPKG
>Mature_587_residues
AKGLTGGQLIAKMLAAEAVDQVFGIIDGTYFGLYRALGDEGITLHSPRHEAAAAHMAGAYARVSGRLGVCMASNGPGVAN
VLPGVAVEQGEGNRILLITSGRRVGITHPDRGGTYQNFDQTAVTAPMAKWSCHVPSADRLPEILRRALRVSFSGRPGVVH
VDVPENIVNQPTGLDAGAVRAPESYRRTTPLQPDPVLVEKAAQLLVEAEWPMIHAGSGVYHAGAEAELARLAGLLAAPVT
TSWAARGALSEARPEAIPMTALGVNDEVRNAADVALVVGSRLGETDWWGKAPNWAPPALQRTIQVDVDDERLGVNKPVSL
AILADAREALRALADAVERRTVPGLAARRDRLEVWRGKVADERAKLAKAVKPGSPVHPGHVPSVAQQVMPDDTVWVFDGG
NTVVWSNFHHSADVPRTILSTYKFGMLGAGMGQALGAAVAAPDQRVCVLIGDGAFGMHPTEVESAVRLGLPIVYVVLVDG
AWGMVKMSQQIQARPIATVAHKMLTNTTLPADQIVYADFAPCRYDLMGEAMGAHGELVTDSADLPAALERARDCGRAAVV
QVHVDQVEHLWAPGLQAFKKMHQEPKG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=489, Percent_Identity=26.9938650306748, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI21361361, Length=526, Percent_Identity=26.9961977186312, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI87081685, Length=585, Percent_Identity=26.8376068376068, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1790104, Length=581, Percent_Identity=27.5387263339071, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788716, Length=479, Percent_Identity=26.9311064718163, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787096, Length=581, Percent_Identity=26.6781411359725, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1786717, Length=490, Percent_Identity=25.1020408163265, Blast_Score=129, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=611, Percent_Identity=25.69558101473, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=24.1563055062167, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=24.1563055062167, Blast_Score=112, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=26.9360269360269, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320816, Length=561, Percent_Identity=25.4901960784314, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=28.7456445993031, Blast_Score=166, Evalue=4e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62452; Mature: 62321

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGLTGGQLIAKMLAAEAVDQVFGIIDGTYFGLYRALGDEGITLHSPRHEAAAAHMAGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHH
YARVSGRLGVCMASNGPGVANVLPGVAVEQGEGNRILLITSGRRVGITHPDRGGTYQNFD
HHEECCCEEEEEECCCCCHHHHCCCCEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCC
QTAVTAPMAKWSCHVPSADRLPEILRRALRVSFSGRPGVVHVDVPENIVNQPTGLDAGAV
CHHHHCCCHHCEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCCCCCCCCCC
RAPESYRRTTPLQPDPVLVEKAAQLLVEAEWPMIHAGSGVYHAGAEAELARLAGLLAAPV
CCCHHHHCCCCCCCCHHHHHHHHHHHEECCCCEEECCCCCEECCCHHHHHHHHHHHHCCC
TTSWAARGALSEARPEAIPMTALGVNDEVRNAADVALVVGSRLGETDWWGKAPNWAPPAL
HHHHHHHCCHHHCCCCCCCEEEECCCHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCHHH
QRTIQVDVDDERLGVNKPVSLAILADAREALRALADAVERRTVPGLAARRDRLEVWRGKV
CEEEEEEECHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
ADERAKLAKAVKPGSPVHPGHVPSVAQQVMPDDTVWVFDGGNTVVWSNFHHSADVPRTIL
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCHHHHH
STYKFGMLGAGMGQALGAAVAAPDQRVCVLIGDGAFGMHPTEVESAVRLGLPIVYVVLVD
HHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEC
GAWGMVKMSQQIQARPIATVAHKMLTNTTLPADQIVYADFAPCRYDLMGEAMGAHGELVT
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHEEEECCCCCHHHHHHHHCCCCCCEEE
DSADLPAALERARDCGRAAVVQVHVDQVEHLWAPGLQAFKKMHQEPKG
CCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
AKGLTGGQLIAKMLAAEAVDQVFGIIDGTYFGLYRALGDEGITLHSPRHEAAAAHMAGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHH
YARVSGRLGVCMASNGPGVANVLPGVAVEQGEGNRILLITSGRRVGITHPDRGGTYQNFD
HHEECCCEEEEEECCCCCHHHHCCCCEEECCCCCEEEEEECCCEEECCCCCCCCCCCCCC
QTAVTAPMAKWSCHVPSADRLPEILRRALRVSFSGRPGVVHVDVPENIVNQPTGLDAGAV
CHHHHCCCHHCEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCCCCCCCCCC
RAPESYRRTTPLQPDPVLVEKAAQLLVEAEWPMIHAGSGVYHAGAEAELARLAGLLAAPV
CCCHHHHCCCCCCCCHHHHHHHHHHHEECCCCEEECCCCCEECCCHHHHHHHHHHHHCCC
TTSWAARGALSEARPEAIPMTALGVNDEVRNAADVALVVGSRLGETDWWGKAPNWAPPAL
HHHHHHHCCHHHCCCCCCCEEEECCCHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCHHH
QRTIQVDVDDERLGVNKPVSLAILADAREALRALADAVERRTVPGLAARRDRLEVWRGKV
CEEEEEEECHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
ADERAKLAKAVKPGSPVHPGHVPSVAQQVMPDDTVWVFDGGNTVVWSNFHHSADVPRTIL
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCHHHHH
STYKFGMLGAGMGQALGAAVAAPDQRVCVLIGDGAFGMHPTEVESAVRLGLPIVYVVLVD
HHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEC
GAWGMVKMSQQIQARPIATVAHKMLTNTTLPADQIVYADFAPCRYDLMGEAMGAHGELVT
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHEEEECCCCCHHHHHHHHCCCCCCEEE
DSADLPAALERARDCGRAAVVQVHVDQVEHLWAPGLQAFKKMHQEPKG
CCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]