Click here to switch to the map view.

The map label for this gene is galE [C]

Identifier: 119717529

GI number: 119717529

Start: 3511207

End: 3512151

Strand: Reverse

Name: galE [C]

Synonym: Noca_3305

Alternate gene names: 119717529

Gene position: 3512151-3511207 (Counterclockwise)

Preceding gene: 119717530

Following gene: 119717528

Centisome position: 70.44

GC content: 73.12

Gene sequence:

>945_bases
ATGACCGTCCTCGTGACCGGTGGCACCGGCTACCTCGGCTCCCTCGTCGTGCGCCGGCTGGCCGACCGGGGGATCGGGAC
CGTGTCGGTCGACGTTCGTGACGCGCGCGACCCCGTCCCCGGGGTCACCTACGTGACCGCAGACCTGCGTGGGCTCGACC
TGGCGCTTCTGATGGGTGAGCACGAGGTCGACGCGGTGGTGCACCTCGCGGCGATCGTGGAGCCCCCCAAGGGGATGTCC
GAGGCGGAGCTCGAGGACATCGAGGTCGGCGGCACCCAGCGCGTGGTCGACGGCTGCCTGGCTGCGGGCGTCGGGCACCT
GACCGTGACCAGCTCGGGCGCGGCGTACGGCTATCTCGCCCGCAACGACGGCCGCCTGCTCACCGAGGACCACCCGGTCC
CCGGCAGCGACGAGTTCGCGTACTCCCGGCACAAGGCCGCCGTCGAGGCGCTGCTCGCGGGGGCCCGTCGGCTGCATCCC
GAGCTCGGGCAGCTGGTGCTGCGTCCGGGGACCATCCTCGGCGCGCACACCGACAACCAGATCACCGCGCTCTTCACGGG
GCGGATCGTGATGACGCTCGCCGAGACCGACGGTCCGTTCACGTTCATCTGGGACGAGGACGTGGCCGACATCGTCGTGG
CCGGCGTGCTGGCCCGGCGCACCGGGACCTACAACCTCGCGGGCGACGGCGTGATGACGCTGCGCGACATCGCCGCGGTG
GAGGGCACCCGCGTCGTGGCGGTGCCGGCCGTGGTCGTGCGGACCGCGCTGGCCGCGATGAAGCGGCTCGGGGTCGGCCG
CTACGGCCCCGAGCAGGTGGAGTTCCTGCTGCACCGCCCGGTGCTCGACAACAGCCGGCTGCGCCGCGACTTCCCGGGGC
TCCCGACCCTGACGACCCGCGAGACCTACGAGGTCTTCCGCCAGGGCCGGTCGGGACGGGGCTGA

Upstream 100 bases:

>100_bases
CGCCGTGGTGCAGGTGCACGTCGACCAGGTCGAGCACCTCTGGGCGCCGGGCCTGCAAGCGTTCAAGAAGATGCACCAGG
AGCCGAAGGGCTGACCCGCG

Downstream 100 bases:

>100_bases
CGTGGCGACCGGATCGGCGGGGGTGGCGATGGTGACCGGCGCGAGCGGCCGGATCGGCCGGCACGTCGTCGACCGGCTGA
TCGAGCGCGGGTACGCCGTG

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTVLVTGGTGYLGSLVVRRLADRGIGTVSVDVRDARDPVPGVTYVTADLRGLDLALLMGEHEVDAVVHLAAIVEPPKGMS
EAELEDIEVGGTQRVVDGCLAAGVGHLTVTSSGAAYGYLARNDGRLLTEDHPVPGSDEFAYSRHKAAVEALLAGARRLHP
ELGQLVLRPGTILGAHTDNQITALFTGRIVMTLAETDGPFTFIWDEDVADIVVAGVLARRTGTYNLAGDGVMTLRDIAAV
EGTRVVAVPAVVVRTALAAMKRLGVGRYGPEQVEFLLHRPVLDNSRLRRDFPGLPTLTTRETYEVFRQGRSGRG

Sequences:

>Translated_314_residues
MTVLVTGGTGYLGSLVVRRLADRGIGTVSVDVRDARDPVPGVTYVTADLRGLDLALLMGEHEVDAVVHLAAIVEPPKGMS
EAELEDIEVGGTQRVVDGCLAAGVGHLTVTSSGAAYGYLARNDGRLLTEDHPVPGSDEFAYSRHKAAVEALLAGARRLHP
ELGQLVLRPGTILGAHTDNQITALFTGRIVMTLAETDGPFTFIWDEDVADIVVAGVLARRTGTYNLAGDGVMTLRDIAAV
EGTRVVAVPAVVVRTALAAMKRLGVGRYGPEQVEFLLHRPVLDNSRLRRDFPGLPTLTTRETYEVFRQGRSGRG
>Mature_313_residues
TVLVTGGTGYLGSLVVRRLADRGIGTVSVDVRDARDPVPGVTYVTADLRGLDLALLMGEHEVDAVVHLAAIVEPPKGMSE
AELEDIEVGGTQRVVDGCLAAGVGHLTVTSSGAAYGYLARNDGRLLTEDHPVPGSDEFAYSRHKAAVEALLAGARRLHPE
LGQLVLRPGTILGAHTDNQITALFTGRIVMTLAETDGPFTFIWDEDVADIVVAGVLARRTGTYNLAGDGVMTLRDIAAVE
GTRVVAVPAVVVRTALAAMKRLGVGRYGPEQVEFLLHRPVLDNSRLRRDFPGLPTLTTRETYEVFRQGRSGRG

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=192, Percent_Identity=28.6458333333333, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 33467; Mature: 33336

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLVTGGTGYLGSLVVRRLADRGIGTVSVDVRDARDPVPGVTYVTADLRGLDLALLMGE
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCEEEEEECC
HEVDAVVHLAAIVEPPKGMSEAELEDIEVGGTQRVVDGCLAAGVGHLTVTSSGAAYGYLA
CHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEE
RNDGRLLTEDHPVPGSDEFAYSRHKAAVEALLAGARRLHPELGQLVLRPGTILGAHTDNQ
ECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHEEECCCEEEECCCCCC
ITALFTGRIVMTLAETDGPFTFIWDEDVADIVVAGVLARRTGTYNLAGDGVMTLRDIAAV
EEEEEECEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHH
EGTRVVAVPAVVVRTALAAMKRLGVGRYGPEQVEFLLHRPVLDNSRLRRDFPGLPTLTTR
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCH
ETYEVFRQGRSGRG
HHHHHHHCCCCCCC
>Mature Secondary Structure 
TVLVTGGTGYLGSLVVRRLADRGIGTVSVDVRDARDPVPGVTYVTADLRGLDLALLMGE
EEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCEEEEEECC
HEVDAVVHLAAIVEPPKGMSEAELEDIEVGGTQRVVDGCLAAGVGHLTVTSSGAAYGYLA
CHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEE
RNDGRLLTEDHPVPGSDEFAYSRHKAAVEALLAGARRLHPELGQLVLRPGTILGAHTDNQ
ECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHEEECCCEEEECCCCCC
ITALFTGRIVMTLAETDGPFTFIWDEDVADIVVAGVLARRTGTYNLAGDGVMTLRDIAAV
EEEEEECEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHH
EGTRVVAVPAVVVRTALAAMKRLGVGRYGPEQVEFLLHRPVLDNSRLRRDFPGLPTLTTR
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCH
ETYEVFRQGRSGRG
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]