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The map label for this gene is yngI [H]

Identifier: 119716950

GI number: 119716950

Start: 2898636

End: 2900282

Strand: Reverse

Name: yngI [H]

Synonym: Noca_2725

Alternate gene names: 119716950

Gene position: 2900282-2898636 (Counterclockwise)

Preceding gene: 119716953

Following gene: 119716949

Centisome position: 58.17

GC content: 69.34

Gene sequence:

>1647_bases
GTGGCTCACAACATCGCTGACCTCTTCGAACACGCCGTCGATGCCGCGCCCGACAACCGCGCCCTCAAGGTCGGTGACCG
GGTCGTCACCTACGCGGACCTGGAGGCCGACGCCAACCGCCTCGCGCACTACCTCCGGGCGCAGGGTGTCGGGGTCGGGG
ACCACGTGGCGATCTACGCCAAGAACAGCATCGAGCACGTCGTGGCGGTGCTCGCGGTCGTCAAGATCCGCGCCGTCAAC
ATCAACGTGAACTACCGCTACGTCGAGGCCGAGCTCGACTACCTCCTCGACAACGCGGATGTGGTCGCGGTGATCCACGA
ACGCACCTACGCCCCGCTGGTTGCCGCGTGCTTCCCGAAGCACGCGAAGCTGCGCCTCGCCGTGGCGATGCGCGACGCCA
TCGAGCCGGAGGACACCTCCGACGTGTCGTCGTACGGCGGCGTCCTGTGGGAGGACGCGCTCGCCGGCCAGAGTGCCGAG
CGGGACTTCGGCGAGCGCAGTGCGGACGACATCCACATCATCTACACCGGTGGGACCACCGGGTTCCCCAAGGGCGTGAT
GTGGCGCCACGAGGACTTCTGGCGGGTGCTCGGCGGCGGCATCGATTTCTACACCGGTGCTCCGCTCGAGGAGTACGACC
AGTCCAAGCAGGCCAACGACCCCCGGATGGTCACCTTCCCGCTGAGCCCGCTGATGCACGGCGGCGCCCAGGCGGGCCTG
CTGATGCACCTGTTCGCCGGGCACCTGACGATCCTGGAGCCCAGGTTCGACCCCGTGCGCACCTGGCAGATCATCGACGA
GAACCAGGTCCAGCTGATCTTCATGACCGGCGACGCCATGGCCCGCCCCCTCATCGAGGCCTACGAGGCCGGCGGCTACG
ACGGGTCCAGCCTGTTCGCGATCTCGAGCAGCGCCGCCATCTTCAGCCCGCCGGTGAAGAAGCGCTGGATGGACGCCTTC
CCCAACGCGGTGTTCACCGACTCGATCGGCGCCTCGGAGACCGGCTTCCAGGGGATGGGCATGCAGGAGAAGGACAACAT
CAGCCCGGACGGCCCGGTCGTCGGCCTCGGCCCGCACAGCGTGGTCATCGACGAGGACGGCCGGGTCCTGGACGTCACCA
CGGACATCGGGAAGGTCGGCCGGCTCGGCCGCGGTGGCAGCGTCCCCGTCGGCTACTACAAGGACCCGGCGAAGTCGGCC
ACCACCTTCCTCACCATCGGCGGCGAGCGCTACTCCGTCCCGGGCGACTTCGCCCGGATCGAGGAGGGCGGCCGGGTCAC
CCTCCTGGGCCGTGGCTCCAACTGCGTGAACACCGGCGGCGAGAAGGTCTACCCCGAGGAGGTCGAGATGGCCGTCAAGG
GACACCCCTCCGTGTACGACGTGCTCGTCGTCGGCATCCCCCACGAGCGCTACGGCCAGGCTGTGGCCGCGGTCGTGGAG
CTGCGCGAGGGCGCCTCCCTCGAGCTGGAGGAGCTGCGGGGCTACCTGCGCGCGCACCTGTCGGGCTACAAGCTGCCGCG
CGCCCTCACGATCGTCGACCGGATCCCGCGCAACGCCACCGGCAAGGCGCAGTACCCGAAGGCCAAGGAGCTGGCGATGG
CCGGCTTCGCCGCCACGGCACCGACCGTCGACCCGACCTCGGTCTGA

Upstream 100 bases:

>100_bases
TGGCCGTCCTGCTCGACGAGGCAGTGGGGGGCGTCCGTGGCTGGGGAGGTCATGGCGCGGAGCCTAGCCAAAAACGAGAA
CGTGTTCTAGTCTCGCGCTC

Downstream 100 bases:

>100_bases
GGCTCCCTCGATCTGTAGGAGAGATGACCATGCGCACCCCGATCTGCGACACGTTCGGGATCGACTACCCGATCTTCGCC
TTCACCCCGTCCGAGCACGT

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAHNIADLFEHAVDAAPDNRALKVGDRVVTYADLEADANRLAHYLRAQGVGVGDHVAIYAKNSIEHVVAVLAVVKIRAVN
INVNYRYVEAELDYLLDNADVVAVIHERTYAPLVAACFPKHAKLRLAVAMRDAIEPEDTSDVSSYGGVLWEDALAGQSAE
RDFGERSADDIHIIYTGGTTGFPKGVMWRHEDFWRVLGGGIDFYTGAPLEEYDQSKQANDPRMVTFPLSPLMHGGAQAGL
LMHLFAGHLTILEPRFDPVRTWQIIDENQVQLIFMTGDAMARPLIEAYEAGGYDGSSLFAISSSAAIFSPPVKKRWMDAF
PNAVFTDSIGASETGFQGMGMQEKDNISPDGPVVGLGPHSVVIDEDGRVLDVTTDIGKVGRLGRGGSVPVGYYKDPAKSA
TTFLTIGGERYSVPGDFARIEEGGRVTLLGRGSNCVNTGGEKVYPEEVEMAVKGHPSVYDVLVVGIPHERYGQAVAAVVE
LREGASLELEELRGYLRAHLSGYKLPRALTIVDRIPRNATGKAQYPKAKELAMAGFAATAPTVDPTSV

Sequences:

>Translated_548_residues
MAHNIADLFEHAVDAAPDNRALKVGDRVVTYADLEADANRLAHYLRAQGVGVGDHVAIYAKNSIEHVVAVLAVVKIRAVN
INVNYRYVEAELDYLLDNADVVAVIHERTYAPLVAACFPKHAKLRLAVAMRDAIEPEDTSDVSSYGGVLWEDALAGQSAE
RDFGERSADDIHIIYTGGTTGFPKGVMWRHEDFWRVLGGGIDFYTGAPLEEYDQSKQANDPRMVTFPLSPLMHGGAQAGL
LMHLFAGHLTILEPRFDPVRTWQIIDENQVQLIFMTGDAMARPLIEAYEAGGYDGSSLFAISSSAAIFSPPVKKRWMDAF
PNAVFTDSIGASETGFQGMGMQEKDNISPDGPVVGLGPHSVVIDEDGRVLDVTTDIGKVGRLGRGGSVPVGYYKDPAKSA
TTFLTIGGERYSVPGDFARIEEGGRVTLLGRGSNCVNTGGEKVYPEEVEMAVKGHPSVYDVLVVGIPHERYGQAVAAVVE
LREGASLELEELRGYLRAHLSGYKLPRALTIVDRIPRNATGKAQYPKAKELAMAGFAATAPTVDPTSV
>Mature_547_residues
AHNIADLFEHAVDAAPDNRALKVGDRVVTYADLEADANRLAHYLRAQGVGVGDHVAIYAKNSIEHVVAVLAVVKIRAVNI
NVNYRYVEAELDYLLDNADVVAVIHERTYAPLVAACFPKHAKLRLAVAMRDAIEPEDTSDVSSYGGVLWEDALAGQSAER
DFGERSADDIHIIYTGGTTGFPKGVMWRHEDFWRVLGGGIDFYTGAPLEEYDQSKQANDPRMVTFPLSPLMHGGAQAGLL
MHLFAGHLTILEPRFDPVRTWQIIDENQVQLIFMTGDAMARPLIEAYEAGGYDGSSLFAISSSAAIFSPPVKKRWMDAFP
NAVFTDSIGASETGFQGMGMQEKDNISPDGPVVGLGPHSVVIDEDGRVLDVTTDIGKVGRLGRGGSVPVGYYKDPAKSAT
TFLTIGGERYSVPGDFARIEEGGRVTLLGRGSNCVNTGGEKVYPEEVEMAVKGHPSVYDVLVVGIPHERYGQAVAAVVEL
REGASLELEELRGYLRAHLSGYKLPRALTIVDRIPRNATGKAQYPKAKELAMAGFAATAPTVDPTSV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=564, Percent_Identity=23.2269503546099, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI187761345, Length=550, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI187761343, Length=550, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI42544132, Length=514, Percent_Identity=22.568093385214, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI58082049, Length=555, Percent_Identity=23.7837837837838, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI157311624, Length=555, Percent_Identity=23.4234234234234, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI157311622, Length=555, Percent_Identity=23.4234234234234, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI28416953, Length=527, Percent_Identity=23.5294117647059, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI38505220, Length=559, Percent_Identity=22.7191413237925, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=22.9287090558767, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI122937307, Length=210, Percent_Identity=30, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788107, Length=563, Percent_Identity=24.3339253996448, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI145693145, Length=559, Percent_Identity=23.613595706619, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790505, Length=517, Percent_Identity=26.4990328820116, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1786810, Length=530, Percent_Identity=22.8301886792453, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI221142682, Length=501, Percent_Identity=23.9520958083832, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=25.0478011472275, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=570, Percent_Identity=24.7368421052632, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=569, Percent_Identity=24.4288224956063, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=570, Percent_Identity=24.7368421052632, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560308, Length=530, Percent_Identity=24.7169811320755, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=24.3445692883895, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=512, Percent_Identity=23.828125, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=519, Percent_Identity=21.3872832369942, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=154, Percent_Identity=33.1168831168831, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=508, Percent_Identity=22.8346456692913, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=569, Percent_Identity=24.780316344464, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=569, Percent_Identity=24.780316344464, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=497, Percent_Identity=25.9557344064386, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323182, Length=575, Percent_Identity=25.0434782608696, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=532, Percent_Identity=22.9323308270677, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=25.4237288135593, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI18859661, Length=533, Percent_Identity=26.078799249531, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21355181, Length=493, Percent_Identity=22.5152129817444, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648253, Length=523, Percent_Identity=23.7093690248566, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 59255; Mature: 59123

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHNIADLFEHAVDAAPDNRALKVGDRVVTYADLEADANRLAHYLRAQGVGVGDHVAIYA
CCCCHHHHHHHHHCCCCCCCEEEECCEEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEE
KNSIEHVVAVLAVVKIRAVNINVNYRYVEAELDYLLDNADVVAVIHERTYAPLVAACFPK
CCCHHHHHHHHHHHEEEEEEEEEEEEEEEEEEHHHCCCCCEEEEEECCCCCHHHHHHCCC
HAKLRLAVAMRDAIEPEDTSDVSSYGGVLWEDALAGQSAERDFGERSADDIHIIYTGGTT
CCEEEEEEEHHHCCCCCCCCCHHHHCCEEEHHHHCCCCHHHHCCCCCCCCEEEEEECCCC
GFPKGVMWRHEDFWRVLGGGIDFYTGAPLEEYDQSKQANDPRMVTFPLSPLMHGGAQAGL
CCCCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCEEEEEECCHHHCCCCHHHH
LMHLFAGHLTILEPRFDPVRTWQIIDENQVQLIFMTGDAMARPLIEAYEAGGYDGSSLFA
HHHHHHCCEEEECCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEEE
ISSSAAIFSPPVKKRWMDAFPNAVFTDSIGASETGFQGMGMQEKDNISPDGPVVGLGPHS
EECCCEEECCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCE
VVIDEDGRVLDVTTDIGKVGRLGRGGSVPVGYYKDPAKSATTFLTIGGERYSVPGDFARI
EEECCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEECCCCCHHHH
EEGGRVTLLGRGSNCVNTGGEKVYPEEVEMAVKGHPSVYDVLVVGIPHERYGQAVAAVVE
CCCCEEEEEECCCCCCCCCCCEECHHHHHHEECCCCCEEEEEEEECCCHHHHHHHHHHHH
LREGASLELEELRGYLRAHLSGYKLPRALTIVDRIPRNATGKAQYPKAKELAMAGFAATA
HHCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCCCCCCCHHHHHHHCCCCCC
PTVDPTSV
CCCCCCCC
>Mature Secondary Structure 
AHNIADLFEHAVDAAPDNRALKVGDRVVTYADLEADANRLAHYLRAQGVGVGDHVAIYA
CCCHHHHHHHHHCCCCCCCEEEECCEEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEE
KNSIEHVVAVLAVVKIRAVNINVNYRYVEAELDYLLDNADVVAVIHERTYAPLVAACFPK
CCCHHHHHHHHHHHEEEEEEEEEEEEEEEEEEHHHCCCCCEEEEEECCCCCHHHHHHCCC
HAKLRLAVAMRDAIEPEDTSDVSSYGGVLWEDALAGQSAERDFGERSADDIHIIYTGGTT
CCEEEEEEEHHHCCCCCCCCCHHHHCCEEEHHHHCCCCHHHHCCCCCCCCEEEEEECCCC
GFPKGVMWRHEDFWRVLGGGIDFYTGAPLEEYDQSKQANDPRMVTFPLSPLMHGGAQAGL
CCCCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCEEEEEECCHHHCCCCHHHH
LMHLFAGHLTILEPRFDPVRTWQIIDENQVQLIFMTGDAMARPLIEAYEAGGYDGSSLFA
HHHHHHCCEEEECCCCCCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCCCEEEE
ISSSAAIFSPPVKKRWMDAFPNAVFTDSIGASETGFQGMGMQEKDNISPDGPVVGLGPHS
EECCCEEECCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCE
VVIDEDGRVLDVTTDIGKVGRLGRGGSVPVGYYKDPAKSATTFLTIGGERYSVPGDFARI
EEECCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEECCCCCHHHH
EEGGRVTLLGRGSNCVNTGGEKVYPEEVEMAVKGHPSVYDVLVVGIPHERYGQAVAAVVE
CCCCEEEEEECCCCCCCCCCCEECHHHHHHEECCCCCEEEEEEEECCCHHHHHHHHHHHH
LREGASLELEELRGYLRAHLSGYKLPRALTIVDRIPRNATGKAQYPKAKELAMAGFAATA
HHCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCCCCCCCHHHHHHHCCCCCC
PTVDPTSV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]