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The map label for this gene is ysgA [H]

Identifier: 119716688

GI number: 119716688

Start: 2620178

End: 2620891

Strand: Direct

Name: ysgA [H]

Synonym: Noca_2462

Alternate gene names: 119716688

Gene position: 2620178-2620891 (Clockwise)

Preceding gene: 119716687

Following gene: 119716689

Centisome position: 52.55

GC content: 73.53

Gene sequence:

>714_bases
GTGCGAACCGAGCGGCGGCTCTTCCTTGCCGACGGCCCGAAGGCCGTCGAGGGTGCCCTCGCCGCCGACGTGGTCGTCGA
GCTCTTCGCGACGCCGGCGGCAGCGGAGCAGTACGCCGACCTGCTCGGTGGCTTCCCGGCGGCGTTGGTCGACGACGGCG
CCATGGCGGGGCTCTCCGACAGCGTGACCCCGGCGGGTCTGGTGGCCGTGTGCCGGTTCCTCGACGACGAGCCCGTGGTC
GACGCCCGGGTGTCGCGGTTGTTGGCGATCTGCGCCGACGTACGCGATCCCGGCAACGCCGGCACGGTCATCCGCTGCGC
CGATGCGGTCGGCGGCGACGGCGTCGTGCTCGCCGGGAACTCGGTCGACCCCTACAACCCCAAGACGGTCCGCGCCAGCG
TCGGCAGCCTGTTCCACGTGCGGATCGGGCAGGAGCGGCACCCCGCGACGGCGGTGTCGCACGCCCGCTCCGCCGGCTTC
ACGGTGCTCGCCGCCGACGGCGCGGGAGACGTCGATCTCTTCGACGCCGACGAGTTGCTGGCGGGTCCGTCCGCCTGGCT
GTTCGGCAACGAGGCCTGGGGCCTCCCCGAGGAGCTCGCGGCGTTGGCCGACCACCGGGTGCGGATCCCGATCTACGGCC
GCGCCGAGAGCCTCAACCTGTCCACCGCGGCGGCACTCTGCCTTTACGCCTCGGCCCGGGTCCAGCGTCGGTAA

Upstream 100 bases:

>100_bases
CGGACGTCAACGCGCCCAAGGCGGAGGCCTCGGCCTGACCACCCCGCTCACGGCCGGCAACGGCCGGGTCAAGGAAGCGC
GCAAGCTCAGCCGCCGCTCG

Downstream 100 bases:

>100_bases
CCTCGCGGCCGTGCCCCCGCGTCGCCTGCTGATCCGACTCGCCCTCGCGCTGAACCTCGCGCTGGCCCTCCCGGTGGCCC
TCGTCGCCCCCGGTGCCGTC

Product: tRNA/rRNA methyltransferase SpoU

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRTERRLFLADGPKAVEGALAADVVVELFATPAAAEQYADLLGGFPAALVDDGAMAGLSDSVTPAGLVAVCRFLDDEPVV
DARVSRLLAICADVRDPGNAGTVIRCADAVGGDGVVLAGNSVDPYNPKTVRASVGSLFHVRIGQERHPATAVSHARSAGF
TVLAADGAGDVDLFDADELLAGPSAWLFGNEAWGLPEELAALADHRVRIPIYGRAESLNLSTAAALCLYASARVQRR

Sequences:

>Translated_237_residues
MRTERRLFLADGPKAVEGALAADVVVELFATPAAAEQYADLLGGFPAALVDDGAMAGLSDSVTPAGLVAVCRFLDDEPVV
DARVSRLLAICADVRDPGNAGTVIRCADAVGGDGVVLAGNSVDPYNPKTVRASVGSLFHVRIGQERHPATAVSHARSAGF
TVLAADGAGDVDLFDADELLAGPSAWLFGNEAWGLPEELAALADHRVRIPIYGRAESLNLSTAAALCLYASARVQRR
>Mature_237_residues
MRTERRLFLADGPKAVEGALAADVVVELFATPAAAEQYADLLGGFPAALVDDGAMAGLSDSVTPAGLVAVCRFLDDEPVV
DARVSRLLAICADVRDPGNAGTVIRCADAVGGDGVVLAGNSVDPYNPKTVRASVGSLFHVRIGQERHPATAVSHARSAGF
TVLAADGAGDVDLFDADELLAGPSAWLFGNEAWGLPEELAALADHRVRIPIYGRAESLNLSTAAALCLYASARVQRR

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=180, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 24577; Mature: 24577

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTERRLFLADGPKAVEGALAADVVVELFATPAAAEQYADLLGGFPAALVDDGAMAGLSD
CCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCC
SVTPAGLVAVCRFLDDEPVVDARVSRLLAICADVRDPGNAGTVIRCADAVGGDGVVLAGN
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEECC
SVDPYNPKTVRASVGSLFHVRIGQERHPATAVSHARSAGFTVLAADGAGDVDLFDADELL
CCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCEEECCHHHH
AGPSAWLFGNEAWGLPEELAALADHRVRIPIYGRAESLNLSTAAALCLYASARVQRR
CCCCEEEECCCCCCCHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTERRLFLADGPKAVEGALAADVVVELFATPAAAEQYADLLGGFPAALVDDGAMAGLSD
CCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCC
SVTPAGLVAVCRFLDDEPVVDARVSRLLAICADVRDPGNAGTVIRCADAVGGDGVVLAGN
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEECC
SVDPYNPKTVRASVGSLFHVRIGQERHPATAVSHARSAGFTVLAADGAGDVDLFDADELL
CCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCEEECCHHHH
AGPSAWLFGNEAWGLPEELAALADHRVRIPIYGRAESLNLSTAAALCLYASARVQRR
CCCCEEEECCCCCCCHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]