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The map label for this gene is metK

Identifier: 119716666

GI number: 119716666

Start: 2600173

End: 2601363

Strand: Direct

Name: metK

Synonym: Noca_2439

Alternate gene names: 119716666

Gene position: 2600173-2601363 (Clockwise)

Preceding gene: 119716665

Following gene: 119716667

Centisome position: 52.15

GC content: 70.28

Gene sequence:

>1191_bases
GTGACCGGACGCCTCTTCACCTCGGAGTCGGTGACCGAGGGTCACCCGGACAAGATCGCCGACCAGATCAGCGACTCGGT
GCTCGACGCCATGCTGGAGCTGGACCCCACCAGCCGGGTCGCGGTCGAGACGCTGCTGACCACCGGCGTCGTGGTCGTCG
CCGGTGAGGTGACCACCACCGGGTACGTCGACGTCAAGGACGTCGTCCGCCGCCGGATCATCGAGATCGGGTACGACTCC
TCGGTCAAGGGCTTCGACGGGCACTCCTGCGGGGTGATGGTGGCGATCGGCGGCCAGTCCGGCGACATCGCGCAGGGCGT
CGACACCGGCCACGAGAGCCGCACCGGCTCGGTCGACGCGATGGACAAGCAAGGTGCCGGCGACCAGGGCCTGATGTTCG
GGTACGCGTGCGACGACACCGACGTGCTGATGCCGCTGCCGATCGTGATCGCCCAGCGGCTCGCCGAGCAGCTCACCGCG
GTCCGCAAGGACGGCACGCTGCCCTACCTGCGCCCCGACGGGAAGACCCAGGTCACCATCGAGTACGACGCCGAGAACCG
CCCGGTGCGGGTCGACACGGTCGTGCTGTCCTCCCAGCACGACGAGGACACCGAGCTCGACACCTTGGAGGCCGACGTCA
AGAAGCACGTCATCGACCCGGTGCTCGCCACCTTCCCGATCCCGTCCGAGGGCTACCGCCTGCTGGTGAATCCCACCGGT
CGTTTCGTCGTCGGCGGCCCGATGGGCGACGCCGGCCTGACCGGCCGCAAGATCATCGTCGACACCTATGGGGGGATGGC
GCGGCACGGCGGCGGTGCGTTCTCCGGCAAGGACCCGTCGAAGGTCGACCGCTCCGCGGCCTACGCGATGCGGTGGGTCG
CGAAGAACGTCGTGGCCGCCGGTCTCGCCACCCGGTGCGAGGCGCAGGTCGCCTATGCCATCGGCAAGGCCCAGCCGGTC
GGCGTCTTCGTCGAGACGTTCGGCACCGGCGTCGTGGACGACGAGCGGATCCAGGAGGCGGTCCTCGAGGTGTTCGACCT
GCGGCCCGCCGCGATCATCCGCGACCTCGATCTGCTCCGGCCGATCTACGCCAAGACCGCGGCGTACGGCCACTTCGGCC
GCGAGCTCCCCGAGTTCACCTGGGAGCGCACCGACCGCGCGGACGCGCTGCGCGCCGCCGCCGGCCTCTAA

Upstream 100 bases:

>100_bases
GAGCAGGCTGCAGGATTGAGACCGCCGTCCCGTGATCCGGGACGCTCGATATCGTTGGCAATCGTTGGCAGACCGCCAAC
CAGGACACAGGAGCGCAGAA

Downstream 100 bases:

>100_bases
CGGCGCCTCGCGCGTCGCCTTCGGCGCCGCGCGGGTCGCGGGGTCGGTCCTCGGGCCGGCCAGTCCTCGTTGTGGTTACG
GTGGGTGCGCGGGTGCGTCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTGRLFTSESVTEGHPDKIADQISDSVLDAMLELDPTSRVAVETLLTTGVVVVAGEVTTTGYVDVKDVVRRRIIEIGYDS
SVKGFDGHSCGVMVAIGGQSGDIAQGVDTGHESRTGSVDAMDKQGAGDQGLMFGYACDDTDVLMPLPIVIAQRLAEQLTA
VRKDGTLPYLRPDGKTQVTIEYDAENRPVRVDTVVLSSQHDEDTELDTLEADVKKHVIDPVLATFPIPSEGYRLLVNPTG
RFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLATRCEAQVAYAIGKAQPV
GVFVETFGTGVVDDERIQEAVLEVFDLRPAAIIRDLDLLRPIYAKTAAYGHFGRELPEFTWERTDRADALRAAAGL

Sequences:

>Translated_396_residues
MTGRLFTSESVTEGHPDKIADQISDSVLDAMLELDPTSRVAVETLLTTGVVVVAGEVTTTGYVDVKDVVRRRIIEIGYDS
SVKGFDGHSCGVMVAIGGQSGDIAQGVDTGHESRTGSVDAMDKQGAGDQGLMFGYACDDTDVLMPLPIVIAQRLAEQLTA
VRKDGTLPYLRPDGKTQVTIEYDAENRPVRVDTVVLSSQHDEDTELDTLEADVKKHVIDPVLATFPIPSEGYRLLVNPTG
RFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLATRCEAQVAYAIGKAQPV
GVFVETFGTGVVDDERIQEAVLEVFDLRPAAIIRDLDLLRPIYAKTAAYGHFGRELPEFTWERTDRADALRAAAGL
>Mature_395_residues
TGRLFTSESVTEGHPDKIADQISDSVLDAMLELDPTSRVAVETLLTTGVVVVAGEVTTTGYVDVKDVVRRRIIEIGYDSS
VKGFDGHSCGVMVAIGGQSGDIAQGVDTGHESRTGSVDAMDKQGAGDQGLMFGYACDDTDVLMPLPIVIAQRLAEQLTAV
RKDGTLPYLRPDGKTQVTIEYDAENRPVRVDTVVLSSQHDEDTELDTLEADVKKHVIDPVLATFPIPSEGYRLLVNPTGR
FVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLATRCEAQVAYAIGKAQPVG
VFVETFGTGVVDDERIQEAVLEVFDLRPAAIIRDLDLLRPIYAKTAAYGHFGRELPEFTWERTDRADALRAAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=383, Percent_Identity=55.0913838120104, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=54.4502617801047, Blast_Score=379, Evalue=1e-105,
Organism=Escherichia coli, GI1789311, Length=396, Percent_Identity=61.1111111111111, Blast_Score=476, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17538494, Length=382, Percent_Identity=53.4031413612565, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17538498, Length=382, Percent_Identity=53.6649214659686, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17551082, Length=382, Percent_Identity=52.6178010471204, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=52.5906735751295, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=48.6910994764398, Blast_Score=320, Evalue=7e-88,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=51.6320474777448, Blast_Score=314, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=50.4398826979472, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI32565903, Length=310, Percent_Identity=51.9354838709677, Blast_Score=287, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=55.421686746988, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=385, Percent_Identity=51.9480519480519, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323209, Length=382, Percent_Identity=53.1413612565445, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=51.1811023622047, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=51.1811023622047, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=51.1811023622047, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=51.1811023622047, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=51.1811023622047, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=51.1811023622047, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=50.6561679790026, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=50.6561679790026, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580541, Length=369, Percent_Identity=49.3224932249323, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=49.6, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_NOCSJ (A1SJF9)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_923631.1
- ProteinModelPortal:   A1SJF9
- SMR:   A1SJF9
- STRING:   A1SJF9
- GeneID:   4598275
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_2439
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   A1SJF9
- ProtClustDB:   PRK05250
- BioCyc:   NSP35761:NOCA_2439-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42385; Mature: 42254

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRLFTSESVTEGHPDKIADQISDSVLDAMLELDPTSRVAVETLLTTGVVVVAGEVTTT
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCEECC
GYVDVKDVVRRRIIEIGYDSSVKGFDGHSCGVMVAIGGQSGDIAQGVDTGHESRTGSVDA
CCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCCCC
MDKQGAGDQGLMFGYACDDTDVLMPLPIVIAQRLAEQLTAVRKDGTLPYLRPDGKTQVTI
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEE
EYDAENRPVRVDTVVLSSQHDEDTELDTLEADVKKHVIDPVLATFPIPSEGYRLLVNPTG
EECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
RFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAA
CEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLATRCEAQVAYAIGKAQPVGVFVETFGTGVVDDERIQEAVLEVFDLRPAAIIRDLDLLR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
PIYAKTAAYGHFGRELPEFTWERTDRADALRAAAGL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TGRLFTSESVTEGHPDKIADQISDSVLDAMLELDPTSRVAVETLLTTGVVVVAGEVTTT
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCEECC
GYVDVKDVVRRRIIEIGYDSSVKGFDGHSCGVMVAIGGQSGDIAQGVDTGHESRTGSVDA
CCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHCCCCCCCCCCCCCCC
MDKQGAGDQGLMFGYACDDTDVLMPLPIVIAQRLAEQLTAVRKDGTLPYLRPDGKTQVTI
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEE
EYDAENRPVRVDTVVLSSQHDEDTELDTLEADVKKHVIDPVLATFPIPSEGYRLLVNPTG
EECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
RFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAA
CEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLATRCEAQVAYAIGKAQPVGVFVETFGTGVVDDERIQEAVLEVFDLRPAAIIRDLDLLR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
PIYAKTAAYGHFGRELPEFTWERTDRADALRAAAGL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA