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The map label for this gene is paaF [C]

Identifier: 119716388

GI number: 119716388

Start: 2306614

End: 2307387

Strand: Reverse

Name: paaF [C]

Synonym: Noca_2157

Alternate gene names: 119716388

Gene position: 2307387-2306614 (Counterclockwise)

Preceding gene: 119716389

Following gene: 119716387

Centisome position: 46.28

GC content: 59.56

Gene sequence:

>774_bases
ATGAGCGAACCCAACATTCAAGTCCACCGGCTCCATGACGTGCTCTACGTCACATTCGACCGGCCAGAAAAGCTGAACGC
CATGACCTGGGAGATGTACGCCCGACTCGAGTCTGCCTGCCGAGAGGCAGAGGCCGACGACGTACGCGCAATGGTTCTGC
AATCCTCGTCACCGCGCGCGTTCATCGCTGGAACGGACATCACCCAGTTCCAATCGTTCGACTCGGGTCGAGACGGCGTC
GACTACGAGCGAAAAATTGACGGAGTCATCGACGCAATCCTGAGCGTTCCGGTGCCAACCATTGCCGCAGTCGACGGCTA
CTGCGTCGGTGGAGGCTTCCTGATCTCCGCCGCATGTGACCTACGCATTGCAACCACGTCGGCACAGTTCGGTGCGCCGA
TGGCACGCACCCTTGGCAACTGTCTTTCTCAACATTCCTACCGGCTCATCGTTGAGCTCCTGGGACGAAGCACAGCGTTC
GACCTCCTCCTGCGAGCACGGCTCATGACTGCTGCCGAGGCTCACCAGCGTGGCTTCGTCGCAGAGCTATGTGCCGCCGG
TCAAATCAGTGTCGTGGTAGAGAGCGAAGCTGAACGACTCAAGAATCATTCGCCGCTCAGCATGCGGGCAGCGAAGACCT
CGCTGAACCGCCTTCTCTTTGAACCCGACCGTAGTAACGACGACGTCCTGGAACAGGTATACGGAAGCCACGACTTTCGC
GAAGCCGTGGAACGCTTCGGAGCGAGACAGGACCCGAAGTGGAGTGGCCGATGA

Upstream 100 bases:

>100_bases
TGCGGTTTTCGAGGACGCCGACAGTCGCAGGTCAGGCTGGGCCGCTTCTGGGCCAGCACACCCAGCAAGTCCTAGCGGAG
TTCGGCGCGGGGGAGGCGTG

Downstream 100 bases:

>100_bases
CCGACGGGGACAACTCGGACCTTCGGCCCAGGCCTGGACCGTTGCGAGGAACCACCGTCATCGACATGACGCGTGCCCTT
GCCGGTCCCCACGCTGCAAT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSEPNIQVHRLHDVLYVTFDRPEKLNAMTWEMYARLESACREAEADDVRAMVLQSSSPRAFIAGTDITQFQSFDSGRDGV
DYERKIDGVIDAILSVPVPTIAAVDGYCVGGGFLISAACDLRIATTSAQFGAPMARTLGNCLSQHSYRLIVELLGRSTAF
DLLLRARLMTAAEAHQRGFVAELCAAGQISVVVESEAERLKNHSPLSMRAAKTSLNRLLFEPDRSNDDVLEQVYGSHDFR
EAVERFGARQDPKWSGR

Sequences:

>Translated_257_residues
MSEPNIQVHRLHDVLYVTFDRPEKLNAMTWEMYARLESACREAEADDVRAMVLQSSSPRAFIAGTDITQFQSFDSGRDGV
DYERKIDGVIDAILSVPVPTIAAVDGYCVGGGFLISAACDLRIATTSAQFGAPMARTLGNCLSQHSYRLIVELLGRSTAF
DLLLRARLMTAAEAHQRGFVAELCAAGQISVVVESEAERLKNHSPLSMRAAKTSLNRLLFEPDRSNDDVLEQVYGSHDFR
EAVERFGARQDPKWSGR
>Mature_256_residues
SEPNIQVHRLHDVLYVTFDRPEKLNAMTWEMYARLESACREAEADDVRAMVLQSSSPRAFIAGTDITQFQSFDSGRDGVD
YERKIDGVIDAILSVPVPTIAAVDGYCVGGGFLISAACDLRIATTSAQFGAPMARTLGNCLSQHSYRLIVELLGRSTAFD
LLLRARLMTAAEAHQRGFVAELCAAGQISVVVESEAERLKNHSPLSMRAAKTSLNRLLFEPDRSNDDVLEQVYGSHDFRE
AVERFGARQDPKWSGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=242, Percent_Identity=26.8595041322314, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4502327, Length=272, Percent_Identity=27.5735294117647, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=28, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221136756, Length=232, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136753, Length=232, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221307494, Length=232, Percent_Identity=25, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI25453481, Length=233, Percent_Identity=24.0343347639485, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI51538573, Length=233, Percent_Identity=24.0343347639485, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4757966, Length=223, Percent_Identity=24.6636771300448, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538581, Length=223, Percent_Identity=24.6636771300448, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45643119, Length=204, Percent_Identity=25.9803921568627, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI260275230, Length=204, Percent_Identity=25.9803921568627, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI260274832, Length=246, Percent_Identity=24.7967479674797, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4757968, Length=233, Percent_Identity=23.6051502145923, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI49355787, Length=233, Percent_Identity=23.6051502145923, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=25.6704980842912, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=27.2277227722772, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082183, Length=271, Percent_Identity=26.1992619926199, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=26.5384615384615, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=25.9398496240602, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788597, Length=203, Percent_Identity=24.6305418719212, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=29.5454545454545, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=25.8064516129032, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=25.3846153846154, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=252, Percent_Identity=26.5873015873016, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=210, Percent_Identity=26.1904761904762, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=24.904214559387, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=240, Percent_Identity=26.25, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=247, Percent_Identity=27.9352226720648, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=24.6031746031746, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=24.6031746031746, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=24.5283018867925, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920382, Length=261, Percent_Identity=30.2681992337165, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21357171, Length=191, Percent_Identity=26.7015706806283, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28415; Mature: 28284

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPNIQVHRLHDVLYVTFDRPEKLNAMTWEMYARLESACREAEADDVRAMVLQSSSPRA
CCCCCEEHEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCCCE
FIAGTDITQFQSFDSGRDGVDYERKIDGVIDAILSVPVPTIAAVDGYCVGGGFLISAACD
EEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCEEEEECCC
LRIATTSAQFGAPMARTLGNCLSQHSYRLIVELLGRSTAFDLLLRARLMTAAEAHQRGFV
EEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
AELCAAGQISVVVESEAERLKNHSPLSMRAAKTSLNRLLFEPDRSNDDVLEQVYGSHDFR
HHHHCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCHHH
EAVERFGARQDPKWSGR
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEPNIQVHRLHDVLYVTFDRPEKLNAMTWEMYARLESACREAEADDVRAMVLQSSSPRA
CCCCEEHEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCCCE
FIAGTDITQFQSFDSGRDGVDYERKIDGVIDAILSVPVPTIAAVDGYCVGGGFLISAACD
EEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCEEEEECCC
LRIATTSAQFGAPMARTLGNCLSQHSYRLIVELLGRSTAFDLLLRARLMTAAEAHQRGFV
EEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHH
AELCAAGQISVVVESEAERLKNHSPLSMRAAKTSLNRLLFEPDRSNDDVLEQVYGSHDFR
HHHHCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCHHH
EAVERFGARQDPKWSGR
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA