Click here to switch to the map view.

The map label for this gene is yiaE [C]

Identifier: 119716178

GI number: 119716178

Start: 2076789

End: 2077703

Strand: Direct

Name: yiaE [C]

Synonym: Noca_1946

Alternate gene names: 119716178

Gene position: 2076789-2077703 (Clockwise)

Preceding gene: 119716177

Following gene: 119716179

Centisome position: 41.65

GC content: 73.66

Gene sequence:

>915_bases
GTGAGCGATCGTCTCGTCTGGCTGCCGTTCGACCCCGCCGAGCTGGGCGACCCGCCGGAGGGCCTGCGCTACCAGGTGGT
CGACCCGACGCAGCACGTCCCCGACACGGTCGGCGACGTCCGGTTCTACGTGCCGCCCTACCAGGTCGGCAGCCGCGTCG
CGGAGGTGCTGCCGCGGATGACCGGGCTCGAGGTGGTCCAGACGGTCACGGCCGGCGTCGACAACCTGCGCGGCCGCGTG
CCGGACGGCGTCACGCTCTGCAACGGCCGTGGCATCCACGACACCTCCACCGCCGAGCTCGCGCTGACCCTGATCCTGAG
CAGCCTGCGCGGGATCCCGGACTTCGTGCGCGCCCAGGCCCGGCACGAGTGGGCGCCCGGGTGGCGCCCGGCGCTGGCCG
ACAAGCGCGTGCTGGTGATCGGGTACGGCGCCATCGGGCGGGCCATCGAGGCCCGGCTGCTGCCGTTCGAGGTCGAGGTC
GTCAGGGTCGCCCGCACCCGACGCGCCGGCGTGCACGGCATCGACGAGCTCCTCGGGCTGCTCCCGGACGCGGACGTCGT
GGTGCTCGTCGTACCCCTCACGGAGGAGACCCGCGGCCTGGTCGACGCGGGCTTCCTGGCCGCGATGAAGGAGGGTGCGC
TGCTGGTCAACGTCGCCCGCGGTGCGGTCGTCGACACCCCTGCCCTGATCGACGCGCTCGACTCGGGTCGGATCCGGGCA
GCGCTCGACGTGACCGACCCCGAGCCGATCCCGGAGGACCACGCGCTCTGGGACGCGCCCGGCCTGCTGATCTCGCCGCA
CGTGGGCGGCGCGAGCAGCGCGATGTGGCCGCGGGCGTACCGGCTGGTGCGCGACCAGCTGCACCGTTACGCGGCGGGGG
AGCGGCTGTGGAACATCATGTCCGACGACTACTGA

Upstream 100 bases:

>100_bases
CAGTTCGACCCAGACGACAGACGGGGCACGCTCTGGTCGGACGGTCCGGCCGCGAACCGCCCCGGGTTCGGTCCCCGGCC
CCGCCGTAGGCTCGACCCTC

Downstream 100 bases:

>100_bases
CCGGGACATAGACTCCGCACCGTGGCAGGGCGGATCCGTGATGAAGACATCGCCGAGGTGCGCGAGAAGGCACGCATCGA
CGACGTCGTCTCGCAGTACG

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSDRLVWLPFDPAELGDPPEGLRYQVVDPTQHVPDTVGDVRFYVPPYQVGSRVAEVLPRMTGLEVVQTVTAGVDNLRGRV
PDGVTLCNGRGIHDTSTAELALTLILSSLRGIPDFVRAQARHEWAPGWRPALADKRVLVIGYGAIGRAIEARLLPFEVEV
VRVARTRRAGVHGIDELLGLLPDADVVVLVVPLTEETRGLVDAGFLAAMKEGALLVNVARGAVVDTPALIDALDSGRIRA
ALDVTDPEPIPEDHALWDAPGLLISPHVGGASSAMWPRAYRLVRDQLHRYAAGERLWNIMSDDY

Sequences:

>Translated_304_residues
MSDRLVWLPFDPAELGDPPEGLRYQVVDPTQHVPDTVGDVRFYVPPYQVGSRVAEVLPRMTGLEVVQTVTAGVDNLRGRV
PDGVTLCNGRGIHDTSTAELALTLILSSLRGIPDFVRAQARHEWAPGWRPALADKRVLVIGYGAIGRAIEARLLPFEVEV
VRVARTRRAGVHGIDELLGLLPDADVVVLVVPLTEETRGLVDAGFLAAMKEGALLVNVARGAVVDTPALIDALDSGRIRA
ALDVTDPEPIPEDHALWDAPGLLISPHVGGASSAMWPRAYRLVRDQLHRYAAGERLWNIMSDDY
>Mature_303_residues
SDRLVWLPFDPAELGDPPEGLRYQVVDPTQHVPDTVGDVRFYVPPYQVGSRVAEVLPRMTGLEVVQTVTAGVDNLRGRVP
DGVTLCNGRGIHDTSTAELALTLILSSLRGIPDFVRAQARHEWAPGWRPALADKRVLVIGYGAIGRAIEARLLPFEVEVV
RVARTRRAGVHGIDELLGLLPDADVVVLVVPLTEETRGLVDAGFLAAMKEGALLVNVARGAVVDTPALIDALDSGRIRAA
LDVTDPEPIPEDHALWDAPGLLISPHVGGASSAMWPRAYRLVRDQLHRYAAGERLWNIMSDDY

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=235, Percent_Identity=34.0425531914894, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI23308577, Length=259, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI145580578, Length=298, Percent_Identity=27.8523489932886, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI4557499, Length=298, Percent_Identity=27.8523489932886, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI61743967, Length=279, Percent_Identity=29.3906810035842, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI4557497, Length=279, Percent_Identity=29.3906810035842, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI145580575, Length=242, Percent_Identity=30.5785123966942, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI87082289, Length=234, Percent_Identity=31.1965811965812, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87081824, Length=224, Percent_Identity=29.4642857142857, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789279, Length=220, Percent_Identity=33.1818181818182, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=255, Percent_Identity=30.5882352941176, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=205, Percent_Identity=34.6341463414634, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322116, Length=246, Percent_Identity=29.2682926829268, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320925, Length=216, Percent_Identity=31.4814814814815, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324964, Length=281, Percent_Identity=25.9786476868327, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324980, Length=195, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325144, Length=156, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=184, Percent_Identity=27.1739130434783, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574286, Length=250, Percent_Identity=31.6, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45552429, Length=253, Percent_Identity=31.2252964426877, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574284, Length=253, Percent_Identity=31.2252964426877, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585514, Length=253, Percent_Identity=31.2252964426877, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28574282, Length=253, Percent_Identity=31.2252964426877, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45551003, Length=253, Percent_Identity=31.2252964426877, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28571528, Length=187, Percent_Identity=35.8288770053476, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24585516, Length=226, Percent_Identity=32.3008849557522, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921140, Length=249, Percent_Identity=31.7269076305221, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24646446, Length=273, Percent_Identity=29.3040293040293, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24646448, Length=273, Percent_Identity=29.3040293040293, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24646452, Length=273, Percent_Identity=29.3040293040293, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24646450, Length=273, Percent_Identity=29.3040293040293, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI62472511, Length=273, Percent_Identity=29.3040293040293, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 33036; Mature: 32905

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLVWLPFDPAELGDPPEGLRYQVVDPTQHVPDTVGDVRFYVPPYQVGSRVAEVLPRM
CCCCEEEEECCHHHCCCCCCCCEEEEECCHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
TGLEVVQTVTAGVDNLRGRVPDGVTLCNGRGIHDTSTAELALTLILSSLRGIPDFVRAQA
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
RHEWAPGWRPALADKRVLVIGYGAIGRAIEARLLPFEVEVVRVARTRRAGVHGIDELLGL
HCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
LPDADVVVLVVPLTEETRGLVDAGFLAAMKEGALLVNVARGAVVDTPALIDALDSGRIRA
CCCCCEEEEEEECCHHHCCHHHHHHHHHHHCCCEEEEECCCCEECCHHHHHHHCCCCEEE
ALDVTDPEPIPEDHALWDAPGLLISPHVGGASSAMWPRAYRLVRDQLHRYAAGERLWNIM
EEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDDY
CCCC
>Mature Secondary Structure 
SDRLVWLPFDPAELGDPPEGLRYQVVDPTQHVPDTVGDVRFYVPPYQVGSRVAEVLPRM
CCCEEEEECCHHHCCCCCCCCEEEEECCHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
TGLEVVQTVTAGVDNLRGRVPDGVTLCNGRGIHDTSTAELALTLILSSLRGIPDFVRAQA
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
RHEWAPGWRPALADKRVLVIGYGAIGRAIEARLLPFEVEVVRVARTRRAGVHGIDELLGL
HCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHH
LPDADVVVLVVPLTEETRGLVDAGFLAAMKEGALLVNVARGAVVDTPALIDALDSGRIRA
CCCCCEEEEEEECCHHHCCHHHHHHHHHHHCCCEEEEECCCCEECCHHHHHHHCCCCEEE
ALDVTDPEPIPEDHALWDAPGLLISPHVGGASSAMWPRAYRLVRDQLHRYAAGERLWNIM
EEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDDY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]