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The map label for this gene is lepA [H]

Identifier: 119716123

GI number: 119716123

Start: 2017233

End: 2019140

Strand: Direct

Name: lepA [H]

Synonym: Noca_1891

Alternate gene names: 119716123

Gene position: 2017233-2019140 (Clockwise)

Preceding gene: 119716120

Following gene: 119716124

Centisome position: 40.46

GC content: 68.5

Gene sequence:

>1908_bases
ATGGCGCCGTCCCCGTCCGCTCGACGAGAGTCACCTGTGCCCACGAACACCGCGCCGCTCCCCGGCCACACCGATCCCGC
GATCATCAGGAACTTCTGCATCATCGCGCACATCGACCACGGCAAGTCGACGCTGGCCGACCGGATGCTGCAGCTGACCG
GTGTCGTCGACGAGCGCAGCGCCCGGGCGCAGTACCTCGACCGGATGGACATCGAGCGCGAGCGCGGCATCACGATCAAG
AGCCAGGCCGTGCGGATGCCGTGGACCGTGCCGGCCGGCAACGCCGCGGGCGCCGAGCCCGGCACCTACGTGCTCAACAT
GATCGACACCCCCGGGCACGTCGACTTCACCTACGAGGTCTCCCGCTCGCTGGAGGCCTGCGAGGCCGCGATCCTGCTGG
TCGACGCCGCCCAGGGCATCGAGGCCCAGACGCTCGCGAACCTCTACCTCGCGATGGGCGCGGACCTGCACATCATCCCG
GTGCTCAACAAGATCGACCTGCCGGGCGCGAACCCCGAGAAGTACGCCGCCGAGCTGGCCGGTCTGGTCGGCTGTGAGCC
CGAGGACGTGCTGCGGGTCAGCGCGAAGACCGGCGACGGCGTGGCGGACCTCCTCAACGAGATCGTCCACCAGACGCCGC
CGCCGGTGGGGGAGGCGGACCGCCCGGCACGGGCGCTGATCTTCGACTCCGTCTACGACACCTACCGCGGCGTGGTCACG
TACGTGCGGGTGGTCGACGGCAGGCTCACCCACCGCGACCGGATCAAGATGATGTCGACGGGCGCGACCCACGAGCTGCT
CGAGCTCGGTGTGATCAGCCCCGAGCAGGTCAAGGCCACCGAGATCGGGGTCGGCGAGGTCGGCTACCTGATCACCGGCG
TGAAGGACGTCCGCCAGTCGCGGGTCGGCGACACCGTCACCACCCAGAACCACGGGGCCACCGAGGCGCTCGGCGGCTAC
AAGCACCCGAACCCGATGGTGTACGCCGGGCTCTACCCGATCGACGGCGACCAGTTCGGCGACCTGCGCGAGGCGCTGGA
GAAGCTCCAGCTCAACGACGCGGCGCTCACCTACGAGCCGGAGACGTCGGGCGCGCTGGGCTTCGGCTTCCGCTGTGGCT
TCCTCGGGCTGCTGCACATGGAGATCACCCGGGACCGGCTGGAGCGGGAGTTCGACCTCGACCTGATCTCCACGGCGCCC
AACGTGGTCTACCACGTCCAGATGGAGGACGGCTCCGAGGTCGAGGTGACCAACCCCAGCGAGTTCCCCGACGGCAAGAT
CGCCGAGATCCGCGAGCCCGTCGTACGCGCCACGATCCTGAGCCCGAGCGACTACATCGGCACGATCATGGAGCTGTGCC
AGACCAAGCGCGGCAGCCTCCAGGGCATGGACTACCTCTCCGAGGACCGCGTGGAGATGCGCTACACGCTGCCGATGGGC
GAGATCGTCTTCGACTTCTTCGACCAGCTGAAGTCGCGCACCAAGGGCTACGCCTCGCTCGACTACGAGCGCTCCGGCGA
GCAGGCGGCCGATCTCGTCAAGGTCGACGTCCTGCTCCAGGGTGAGCCGGTCGACGCGTTCTCGGCGATCGTGCACCGCG
AGGCGGCGTACTCCTACGGCGTGATGATGGCCGGCAAGCTCAAGGAGCTGATCCCGCGCCAGCAGTTCGAGGTGCCGATC
CAGGCCGCCATCGGCGCCCGCGTGATCGCGCGGGAGAACATCCGCGCGATCCGCAAGGACGTGCTCGCCAAGTGCTACGG
CGGCGACATCAGCCGCAAGCGCAAGCTGCTGGAGAAGCAGAAGGCCGGCAAGAAGCGGATGAAGAACATCGGCACCGTCG
AGGTGCCGCCGGAGGCGTTCATCGCCGCGCTCTCCAACGCGCCCACCTCGGAGAAGACCAAGAAGTAG

Upstream 100 bases:

>100_bases
GGACGGCGTACGGCGCTCACCGGGTCATCCTCGCGGATGGGGCCGCGCCGATCGTGCGGGGTCCGTGCGGAGTGCGGGTT
TCGCGGCGCCGAATGGAACA

Downstream 100 bases:

>100_bases
TGATCCCGCCAGGGTTCTCGGCGTACGCCGCGCGCGGCGCGGACTGGGCCGCGTTCCTCGATCGTCTTCCCGGGCTGGTC
CGCGACCTGCTGGAGGAGTG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MAPSPSARRESPVPTNTAPLPGHTDPAIIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSARAQYLDRMDIERERGITIK
SQAVRMPWTVPAGNAAGAEPGTYVLNMIDTPGHVDFTYEVSRSLEACEAAILLVDAAQGIEAQTLANLYLAMGADLHIIP
VLNKIDLPGANPEKYAAELAGLVGCEPEDVLRVSAKTGDGVADLLNEIVHQTPPPVGEADRPARALIFDSVYDTYRGVVT
YVRVVDGRLTHRDRIKMMSTGATHELLELGVISPEQVKATEIGVGEVGYLITGVKDVRQSRVGDTVTTQNHGATEALGGY
KHPNPMVYAGLYPIDGDQFGDLREALEKLQLNDAALTYEPETSGALGFGFRCGFLGLLHMEITRDRLEREFDLDLISTAP
NVVYHVQMEDGSEVEVTNPSEFPDGKIAEIREPVVRATILSPSDYIGTIMELCQTKRGSLQGMDYLSEDRVEMRYTLPMG
EIVFDFFDQLKSRTKGYASLDYERSGEQAADLVKVDVLLQGEPVDAFSAIVHREAAYSYGVMMAGKLKELIPRQQFEVPI
QAAIGARVIARENIRAIRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKNIGTVEVPPEAFIAALSNAPTSEKTKK

Sequences:

>Translated_635_residues
MAPSPSARRESPVPTNTAPLPGHTDPAIIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSARAQYLDRMDIERERGITIK
SQAVRMPWTVPAGNAAGAEPGTYVLNMIDTPGHVDFTYEVSRSLEACEAAILLVDAAQGIEAQTLANLYLAMGADLHIIP
VLNKIDLPGANPEKYAAELAGLVGCEPEDVLRVSAKTGDGVADLLNEIVHQTPPPVGEADRPARALIFDSVYDTYRGVVT
YVRVVDGRLTHRDRIKMMSTGATHELLELGVISPEQVKATEIGVGEVGYLITGVKDVRQSRVGDTVTTQNHGATEALGGY
KHPNPMVYAGLYPIDGDQFGDLREALEKLQLNDAALTYEPETSGALGFGFRCGFLGLLHMEITRDRLEREFDLDLISTAP
NVVYHVQMEDGSEVEVTNPSEFPDGKIAEIREPVVRATILSPSDYIGTIMELCQTKRGSLQGMDYLSEDRVEMRYTLPMG
EIVFDFFDQLKSRTKGYASLDYERSGEQAADLVKVDVLLQGEPVDAFSAIVHREAAYSYGVMMAGKLKELIPRQQFEVPI
QAAIGARVIARENIRAIRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKNIGTVEVPPEAFIAALSNAPTSEKTKK
>Mature_634_residues
APSPSARRESPVPTNTAPLPGHTDPAIIRNFCIIAHIDHGKSTLADRMLQLTGVVDERSARAQYLDRMDIERERGITIKS
QAVRMPWTVPAGNAAGAEPGTYVLNMIDTPGHVDFTYEVSRSLEACEAAILLVDAAQGIEAQTLANLYLAMGADLHIIPV
LNKIDLPGANPEKYAAELAGLVGCEPEDVLRVSAKTGDGVADLLNEIVHQTPPPVGEADRPARALIFDSVYDTYRGVVTY
VRVVDGRLTHRDRIKMMSTGATHELLELGVISPEQVKATEIGVGEVGYLITGVKDVRQSRVGDTVTTQNHGATEALGGYK
HPNPMVYAGLYPIDGDQFGDLREALEKLQLNDAALTYEPETSGALGFGFRCGFLGLLHMEITRDRLEREFDLDLISTAPN
VVYHVQMEDGSEVEVTNPSEFPDGKIAEIREPVVRATILSPSDYIGTIMELCQTKRGSLQGMDYLSEDRVEMRYTLPMGE
IVFDFFDQLKSRTKGYASLDYERSGEQAADLVKVDVLLQGEPVDAFSAIVHREAAYSYGVMMAGKLKELIPRQQFEVPIQ
AAIGARVIARENIRAIRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKNIGTVEVPPEAFIAALSNAPTSEKTKK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=605, Percent_Identity=46.7768595041322, Blast_Score=595, Evalue=1e-170,
Organism=Homo sapiens, GI94966754, Length=145, Percent_Identity=41.3793103448276, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI19923640, Length=186, Percent_Identity=40.8602150537634, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306287, Length=186, Percent_Identity=40.8602150537634, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306283, Length=186, Percent_Identity=40.8602150537634, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI18390331, Length=152, Percent_Identity=36.1842105263158, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=34.4827586206897, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI310132016, Length=120, Percent_Identity=39.1666666666667, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310110807, Length=120, Percent_Identity=39.1666666666667, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310123363, Length=120, Percent_Identity=39.1666666666667, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI217272892, Length=141, Percent_Identity=32.6241134751773, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI217272894, Length=141, Percent_Identity=32.6241134751773, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI53729339, Length=242, Percent_Identity=27.6859504132231, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI53729337, Length=242, Percent_Identity=27.6859504132231, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=54.9413735343384, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=485, Percent_Identity=28.0412371134021, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI1789738, Length=161, Percent_Identity=39.1304347826087, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790835, Length=167, Percent_Identity=29.940119760479, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789559, Length=287, Percent_Identity=27.5261324041812, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=631, Percent_Identity=39.4611727416799, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17533571, Length=171, Percent_Identity=38.5964912280702, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=145, Percent_Identity=38.6206896551724, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=139, Percent_Identity=38.1294964028777, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=152, Percent_Identity=35.5263157894737, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=143, Percent_Identity=34.2657342657343, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=603, Percent_Identity=43.4494195688226, Blast_Score=516, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6324166, Length=147, Percent_Identity=40.1360544217687, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=37.0629370629371, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=37.0629370629371, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323098, Length=189, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=122, Percent_Identity=41.8032786885246, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI78706572, Length=605, Percent_Identity=43.3057851239669, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=41.726618705036, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582462, Length=171, Percent_Identity=34.5029239766082, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=35.3741496598639, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=35.3741496598639, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=35.3741496598639, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=179, Percent_Identity=37.9888268156425, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=145, Percent_Identity=32.4137931034483, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 69546; Mature: 69415

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSPSARRESPVPTNTAPLPGHTDPAIIRNFCIIAHIDHGKSTLADRMLQLTGVVDERS
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHHHHCCHHHHH
ARAQYLDRMDIERERGITIKSQAVRMPWTVPAGNAAGAEPGTYVLNMIDTPGHVDFTYEV
HHHHHHHHHCCHHHCCCEEECCCEECCEECCCCCCCCCCCCCEEEEEECCCCCEEEEHHH
SRSLEACEAAILLVDAAQGIEAQTLANLYLAMGADLHIIPVLNKIDLPGANPEKYAAELA
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
GLVGCEPEDVLRVSAKTGDGVADLLNEIVHQTPPPVGEADRPARALIFDSVYDTYRGVVT
HHCCCCHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YVRVVDGRLTHRDRIKMMSTGATHELLELGVISPEQVKATEIGVGEVGYLITGVKDVRQS
HHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCEECCCCHHHHHHHCHHHHHHH
RVGDTVTTQNHGATEALGGYKHPNPMVYAGLYPIDGDQFGDLREALEKLQLNDAALTYEP
HCCCCEECCCCCCHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECC
ETSGALGFGFRCGFLGLLHMEITRDRLEREFDLDLISTAPNVVYHVQMEDGSEVEVTNPS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEEECCCCEEEECCCC
EFPDGKIAEIREPVVRATILSPSDYIGTIMELCQTKRGSLQGMDYLSEDRVEMRYTLPMG
CCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEECCHH
EIVFDFFDQLKSRTKGYASLDYERSGEQAADLVKVDVLLQGEPVDAFSAIVHREAAYSYG
HHHHHHHHHHHHHCCCCEECCCCCCCHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHH
VMMAGKLKELIPRQQFEVPIQAAIGARVIARENIRAIRKDVLAKCYGGDISRKRKLLEKQ
HHHHHHHHHHCCHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KAGKKRMKNIGTVEVPPEAFIAALSNAPTSEKTKK
HHHHHHHHCCCCEECCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
APSPSARRESPVPTNTAPLPGHTDPAIIRNFCIIAHIDHGKSTLADRMLQLTGVVDERS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHHHHCCHHHHH
ARAQYLDRMDIERERGITIKSQAVRMPWTVPAGNAAGAEPGTYVLNMIDTPGHVDFTYEV
HHHHHHHHHCCHHHCCCEEECCCEECCEECCCCCCCCCCCCCEEEEEECCCCCEEEEHHH
SRSLEACEAAILLVDAAQGIEAQTLANLYLAMGADLHIIPVLNKIDLPGANPEKYAAELA
HHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
GLVGCEPEDVLRVSAKTGDGVADLLNEIVHQTPPPVGEADRPARALIFDSVYDTYRGVVT
HHCCCCHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YVRVVDGRLTHRDRIKMMSTGATHELLELGVISPEQVKATEIGVGEVGYLITGVKDVRQS
HHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCEECCCCHHHHHHHCHHHHHHH
RVGDTVTTQNHGATEALGGYKHPNPMVYAGLYPIDGDQFGDLREALEKLQLNDAALTYEP
HCCCCEECCCCCCHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECC
ETSGALGFGFRCGFLGLLHMEITRDRLEREFDLDLISTAPNVVYHVQMEDGSEVEVTNPS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEEECCCCEEEECCCC
EFPDGKIAEIREPVVRATILSPSDYIGTIMELCQTKRGSLQGMDYLSEDRVEMRYTLPMG
CCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEECCHH
EIVFDFFDQLKSRTKGYASLDYERSGEQAADLVKVDVLLQGEPVDAFSAIVHREAAYSYG
HHHHHHHHHHHHHCCCCEECCCCCCCHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHH
VMMAGKLKELIPRQQFEVPIQAAIGARVIARENIRAIRKDVLAKCYGGDISRKRKLLEKQ
HHHHHHHHHHCCHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KAGKKRMKNIGTVEVPPEAFIAALSNAPTSEKTKK
HHHHHHHHCCCCEECCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA