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The map label for this gene is tgs1 [H]

Identifier: 119715632

GI number: 119715632

Start: 1473826

End: 1475202

Strand: Reverse

Name: tgs1 [H]

Synonym: Noca_1396

Alternate gene names: 119715632

Gene position: 1475202-1473826 (Counterclockwise)

Preceding gene: 119715642

Following gene: 119715631

Centisome position: 29.59

GC content: 75.53

Gene sequence:

>1377_bases
GTGGACCGACTCACACCGCTCGCCGATGCGTTCCTCGAGGTCGAGGACGTCGACCCGGCGGTCTCGCTCGCCATCGGGTC
GCTGGCGGTCCTCGGCGGCCCGGCGCCCCGGTACGACGAGTTCCTGGCGCTGGTCGACGCACGGCTCCCCCTCGTCCCCC
GGTACCGGCAGCGCCTGCGGCGGGTGCCGTTCGACCTGGCCGCACCGGCGTGGGTCGCGGACGAGGCCTTCGACCTGCGC
AACCACGTGATGCGCGCCGCCGTGCCCAGCCCCGGCGGGCCGGGCGAGGTCGCCGGGGTCGTGGCCGCGCTGATGTCGGC
CCGCATGGACCGGTCCCGCCCGCTCTGGGAGTCGTGGCTGATCGAGGGGCTCGCCGACGGGAAGTGGGCGGTGCTCTCCC
GGGTCCACCACTGCCTCGCCGACGGGGTCTCGGGGATCGATCTCTACCGGCTCTTCCTCGACCTGGCCCCGAACGCCGCT
CCGCTGGTGCCGGCACCAGACCCGCATGGCCGCGCGGACACGCCGGCCGCCTTCGCGGCCCGTGGCCTCGGCGAGCTGGT
CGGCTACCCGGCCCGGGTGGCGACCGCGCTGAGCCGGCTCTCCCGCACCCCGGTCCGCTCGGCCAACGCCGTCGCGCACG
CCGCCCGGGGGCTGCTCGACCTGAGCGGCGCGGCCCGGCCCGTGTACCCGTCCTCCCTCCTCGGCCCGATCGGCGCCGAC
CGGCGGTTCGCGTGGGTCGAGGTGCACCTGTCCGAGGTCGCCGACGTACGCCGGCGCTGCGGCGCGAGCGTCAACGACGT
CGCGCTCGCGGCGATCGCCGGCGGGTTCCGTCAGCTGCTGCTCGGCAGGGGCGAGAGCCCCGACGCCCGGACGGTCCGCT
CGCTGGTCCCGGTCTCGACGCGCGCGAAGGGCGACGAGGCGACGACGGACAACCAGGTGTCGCTGCTGCTCCCCTACCTG
CCGGTCGACGTCGCCGACCCCGTCGAGCGGGTGCACGCCGTGCACCGGCGGCTGCGCCGCCTGCGCGCCCGCCACCAGCC
AGAGGCCGGCGTGGGTGTCACCTCGACGGCCGGGCTGGGCCCGTTCCCGCTCGTGTCGTGGGGCCTGCGGACCGCCTGGC
GGTTCCCGCAGCACCAGGTGACGACCGTGACCACGAACGTACCCGGTCCCCCGGTGCCGCTGTACTGCCTGGGCCGACCC
GTGTGCTCCATCGTGCCGTGGGTGCCGATCGCCGACCACCTCCGGGTCGGCGTGGCGATGCTGTCCTACCAGGACGCCCT
GACCTTCGGGCTGACCGGCGACCACGAGTCGATGCCGGATCTCCAGGTCCTGGCCGACGGCATTGCGGAGTCCTGGTCGG
AGCTGACCGCACGGTAG

Upstream 100 bases:

>100_bases
GGCCCGGGACCCTCAGGATCCGCGCCGCCTCGGCGAGGGTCGGTCGGCCCGGGCGCCCGGGGCGGCACCGCGTCGACACT
GGTCGCGGAAGGAGTCCCCG

Downstream 100 bases:

>100_bases
CGTCGGGGCATGACTGATCGCCCCCGGGGGGACGGCGAGCCCGGGGACGGGTCTCCCGACTTCCACTGGCTGTACGGCGG
CTCCGAGGACCCGGGCCGCG

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: TGS1; Probable triacylglycerol synthase tgs1 [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MDRLTPLADAFLEVEDVDPAVSLAIGSLAVLGGPAPRYDEFLALVDARLPLVPRYRQRLRRVPFDLAAPAWVADEAFDLR
NHVMRAAVPSPGGPGEVAGVVAALMSARMDRSRPLWESWLIEGLADGKWAVLSRVHHCLADGVSGIDLYRLFLDLAPNAA
PLVPAPDPHGRADTPAAFAARGLGELVGYPARVATALSRLSRTPVRSANAVAHAARGLLDLSGAARPVYPSSLLGPIGAD
RRFAWVEVHLSEVADVRRRCGASVNDVALAAIAGGFRQLLLGRGESPDARTVRSLVPVSTRAKGDEATTDNQVSLLLPYL
PVDVADPVERVHAVHRRLRRLRARHQPEAGVGVTSTAGLGPFPLVSWGLRTAWRFPQHQVTTVTTNVPGPPVPLYCLGRP
VCSIVPWVPIADHLRVGVAMLSYQDALTFGLTGDHESMPDLQVLADGIAESWSELTAR

Sequences:

>Translated_458_residues
MDRLTPLADAFLEVEDVDPAVSLAIGSLAVLGGPAPRYDEFLALVDARLPLVPRYRQRLRRVPFDLAAPAWVADEAFDLR
NHVMRAAVPSPGGPGEVAGVVAALMSARMDRSRPLWESWLIEGLADGKWAVLSRVHHCLADGVSGIDLYRLFLDLAPNAA
PLVPAPDPHGRADTPAAFAARGLGELVGYPARVATALSRLSRTPVRSANAVAHAARGLLDLSGAARPVYPSSLLGPIGAD
RRFAWVEVHLSEVADVRRRCGASVNDVALAAIAGGFRQLLLGRGESPDARTVRSLVPVSTRAKGDEATTDNQVSLLLPYL
PVDVADPVERVHAVHRRLRRLRARHQPEAGVGVTSTAGLGPFPLVSWGLRTAWRFPQHQVTTVTTNVPGPPVPLYCLGRP
VCSIVPWVPIADHLRVGVAMLSYQDALTFGLTGDHESMPDLQVLADGIAESWSELTAR
>Mature_458_residues
MDRLTPLADAFLEVEDVDPAVSLAIGSLAVLGGPAPRYDEFLALVDARLPLVPRYRQRLRRVPFDLAAPAWVADEAFDLR
NHVMRAAVPSPGGPGEVAGVVAALMSARMDRSRPLWESWLIEGLADGKWAVLSRVHHCLADGVSGIDLYRLFLDLAPNAA
PLVPAPDPHGRADTPAAFAARGLGELVGYPARVATALSRLSRTPVRSANAVAHAARGLLDLSGAARPVYPSSLLGPIGAD
RRFAWVEVHLSEVADVRRRCGASVNDVALAAIAGGFRQLLLGRGESPDARTVRSLVPVSTRAKGDEATTDNQVSLLLPYL
PVDVADPVERVHAVHRRLRRLRARHQPEAGVGVTSTAGLGPFPLVSWGLRTAWRFPQHQVTTVTTNVPGPPVPLYCLGRP
VCSIVPWVPIADHLRVGVAMLSYQDALTFGLTGDHESMPDLQVLADGIAESWSELTAR

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Prefers C(26:0)-CoA over C(18:1)-CoA. TG synthesis activity increases in M.tuberculosis upon oxygen depletion and NO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 49077; Mature: 49077

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLTPLADAFLEVEDVDPAVSLAIGSLAVLGGPAPRYDEFLALVDARLPLVPRYRQRLR
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
RVPFDLAAPAWVADEAFDLRNHVMRAAVPSPGGPGEVAGVVAALMSARMDRSRPLWESWL
CCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
IEGLADGKWAVLSRVHHCLADGVSGIDLYRLFLDLAPNAAPLVPAPDPHGRADTPAAFAA
HHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
RGLGELVGYPARVATALSRLSRTPVRSANAVAHAARGLLDLSGAARPVYPSSLLGPIGAD
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCC
RRFAWVEVHLSEVADVRRRCGASVNDVALAAIAGGFRQLLLGRGESPDARTVRSLVPVST
CEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
RAKGDEATTDNQVSLLLPYLPVDVADPVERVHAVHRRLRRLRARHQPEAGVGVTSTAGLG
CCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFPLVSWGLRTAWRFPQHQVTTVTTNVPGPPVPLYCLGRPVCSIVPWVPIADHLRVGVAM
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHCCCCCHHHHCCCCCCHHHHHHHHHH
LSYQDALTFGLTGDHESMPDLQVLADGIAESWSELTAR
HHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRLTPLADAFLEVEDVDPAVSLAIGSLAVLGGPAPRYDEFLALVDARLPLVPRYRQRLR
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
RVPFDLAAPAWVADEAFDLRNHVMRAAVPSPGGPGEVAGVVAALMSARMDRSRPLWESWL
CCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
IEGLADGKWAVLSRVHHCLADGVSGIDLYRLFLDLAPNAAPLVPAPDPHGRADTPAAFAA
HHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
RGLGELVGYPARVATALSRLSRTPVRSANAVAHAARGLLDLSGAARPVYPSSLLGPIGAD
HHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCC
RRFAWVEVHLSEVADVRRRCGASVNDVALAAIAGGFRQLLLGRGESPDARTVRSLVPVST
CEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
RAKGDEATTDNQVSLLLPYLPVDVADPVERVHAVHRRLRRLRARHQPEAGVGVTSTAGLG
CCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PFPLVSWGLRTAWRFPQHQVTTVTTNVPGPPVPLYCLGRPVCSIVPWVPIADHLRVGVAM
CHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHCCCCCHHHHCCCCCCHHHHHHHHHH
LSYQDALTFGLTGDHESMPDLQVLADGIAESWSELTAR
HHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]