Click here to switch to the map view.

The map label for this gene is yngF [H]

Identifier: 119715630

GI number: 119715630

Start: 1471739

End: 1472605

Strand: Reverse

Name: yngF [H]

Synonym: Noca_1394

Alternate gene names: 119715630

Gene position: 1472605-1471739 (Counterclockwise)

Preceding gene: 119715631

Following gene: 119715628

Centisome position: 29.54

GC content: 72.09

Gene sequence:

>867_bases
ATGAGCTACCAGACCCTCGACTGGTCCGTCGACACGGACGGGATCGCCACACTCACCTTGAACCGGCCCGACCAGCTCAA
CGCCTTCGACGTGACCATGGCGCGCGAGCTCGAGTCGGTCTTCCGCGGCGACGCCTGGTCCGACGACGTGCGCGCGGTCG
TGGTCACCGGCGCCGGCCGCGCGTTCTGCGCCGGGATGGACCTGGCGGTCGAGGGCAACGTGTTCGGGCTCGACGAGTCG
CTGCGCCCGACGCCCGAGGAGTTCCGGGCGTCGTACGACGAGCCGCCGTACCACGACGGGGTGCGCGACACCGGCGGCCG
CGTCACGCTCGCGATCCACGCCCTGCCGAAGCCGGTGATCGCGGCGATCAACGGCCCGGCGGTCGGCATCGGCGCCACGA
TGACGCTGGCGATGGACCTGCGGCTGGCGTCGACGAGGGCCCGGATCGGCTTTGTCTTCGGGCGGCTCGGCATCGTCCCG
GAGGCGGCGTCGACCTGGTTCCTGCCGCGGATCGTGGGCATCCAGCAGGCACTCGAGTGGGTCTACTCGGCGGACATCCT
CACCGCCGACGCCGCGCTCGCCGGCCGCCTGGTGCGCAGCGTGCACGAGCCGGACGAGCTGCTCGCCGCGGCGCACGACC
TCGCCCGTTCCTTCGTGGTCGGTCGGTCCCCCATGGCGCTGGGCCTGGCGAAGCGGCTGCTCTATCGCAACAGCGCGGTC
GCCGACCCCCTCGAGGCGCACCTGTCGGACTCCCTGGCCATGTACTGGGCCTCGATCGGTGACGGGAAGGAGGGCGTCGC
CGCGTTCCTGGAGAAGCGGGCGCCCACCTTCACCGGTCGCGCCTCGGAGCTGCCGCCGATCTCCTAA

Upstream 100 bases:

>100_bases
AGCGCTCCCAGCAGATGTTCCGCTACATCATCGAGGACAGGACCGACGCCATCCCGGCGAGCCTGTGCACGCCCACCGGA
CTCCCGAAGAGCGTGACCGG

Downstream 100 bases:

>100_bases
GCCGCCGGGCCCGAGGACACCGGCGCCTCGTCGGAGGCGGCGAGCATCCGCTCCCGCAGGCGGGCCAGGATCCGGTTCAG
CAGCCGGGAGACCTGCATCT

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSYQTLDWSVDTDGIATLTLNRPDQLNAFDVTMARELESVFRGDAWSDDVRAVVVTGAGRAFCAGMDLAVEGNVFGLDES
LRPTPEEFRASYDEPPYHDGVRDTGGRVTLAIHALPKPVIAAINGPAVGIGATMTLAMDLRLASTRARIGFVFGRLGIVP
EAASTWFLPRIVGIQQALEWVYSADILTADAALAGRLVRSVHEPDELLAAAHDLARSFVVGRSPMALGLAKRLLYRNSAV
ADPLEAHLSDSLAMYWASIGDGKEGVAAFLEKRAPTFTGRASELPPIS

Sequences:

>Translated_288_residues
MSYQTLDWSVDTDGIATLTLNRPDQLNAFDVTMARELESVFRGDAWSDDVRAVVVTGAGRAFCAGMDLAVEGNVFGLDES
LRPTPEEFRASYDEPPYHDGVRDTGGRVTLAIHALPKPVIAAINGPAVGIGATMTLAMDLRLASTRARIGFVFGRLGIVP
EAASTWFLPRIVGIQQALEWVYSADILTADAALAGRLVRSVHEPDELLAAAHDLARSFVVGRSPMALGLAKRLLYRNSAV
ADPLEAHLSDSLAMYWASIGDGKEGVAAFLEKRAPTFTGRASELPPIS
>Mature_287_residues
SYQTLDWSVDTDGIATLTLNRPDQLNAFDVTMARELESVFRGDAWSDDVRAVVVTGAGRAFCAGMDLAVEGNVFGLDESL
RPTPEEFRASYDEPPYHDGVRDTGGRVTLAIHALPKPVIAAINGPAVGIGATMTLAMDLRLASTRARIGFVFGRLGIVPE
AASTWFLPRIVGIQQALEWVYSADILTADAALAGRLVRSVHEPDELLAAAHDLARSFVVGRSPMALGLAKRLLYRNSAVA
DPLEAHLSDSLAMYWASIGDGKEGVAAFLEKRAPTFTGRASELPPIS

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=269, Percent_Identity=26.7657992565056, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=28.7937743190661, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI4502327, Length=276, Percent_Identity=30.7971014492754, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=28.9617486338798, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787660, Length=271, Percent_Identity=35.7933579335793, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=33.5849056603774, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788597, Length=277, Percent_Identity=28.158844765343, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI221142681, Length=273, Percent_Identity=31.1355311355311, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788682, Length=214, Percent_Identity=28.5046728971963, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790281, Length=216, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082183, Length=290, Percent_Identity=25.5172413793103, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=221, Percent_Identity=32.1266968325792, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=233, Percent_Identity=32.1888412017167, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=272, Percent_Identity=27.5735294117647, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=276, Percent_Identity=30.0724637681159, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=28.0701754385965, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=26.6409266409266, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653139, Length=271, Percent_Identity=30.6273062730627, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20129971, Length=267, Percent_Identity=28.8389513108614, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653477, Length=267, Percent_Identity=28.8389513108614, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24654903, Length=209, Percent_Identity=27.7511961722488, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920382, Length=239, Percent_Identity=26.3598326359833, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922422, Length=270, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357171, Length=246, Percent_Identity=26.4227642276423, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 30879; Mature: 30748

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQTLDWSVDTDGIATLTLNRPDQLNAFDVTMARELESVFRGDAWSDDVRAVVVTGAGR
CCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
AFCAGMDLAVEGNVFGLDESLRPTPEEFRASYDEPPYHDGVRDTGGRVTLAIHALPKPVI
EEEECCCEEECCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEE
AAINGPAVGIGATMTLAMDLRLASTRARIGFVFGRLGIVPEAASTWFLPRIVGIQQALEW
EECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VYSADILTADAALAGRLVRSVHEPDELLAAAHDLARSFVVGRSPMALGLAKRLLYRNSAV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
ADPLEAHLSDSLAMYWASIGDGKEGVAAFLEKRAPTFTGRASELPPIS
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SYQTLDWSVDTDGIATLTLNRPDQLNAFDVTMARELESVFRGDAWSDDVRAVVVTGAGR
CCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
AFCAGMDLAVEGNVFGLDESLRPTPEEFRASYDEPPYHDGVRDTGGRVTLAIHALPKPVI
EEEECCCEEECCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEE
AAINGPAVGIGATMTLAMDLRLASTRARIGFVFGRLGIVPEAASTWFLPRIVGIQQALEW
EECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VYSADILTADAALAGRLVRSVHEPDELLAAAHDLARSFVVGRSPMALGLAKRLLYRNSAV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
ADPLEAHLSDSLAMYWASIGDGKEGVAAFLEKRAPTFTGRASELPPIS
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]