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The map label for this gene is pilT [H]

Identifier: 119715368

GI number: 119715368

Start: 1197616

End: 1198716

Strand: Reverse

Name: pilT [H]

Synonym: Noca_1130

Alternate gene names: 119715368

Gene position: 1198716-1197616 (Counterclockwise)

Preceding gene: 119715376

Following gene: 119715367

Centisome position: 24.04

GC content: 72.3

Gene sequence:

>1101_bases
TTGCGGATCGCGCCCTTCCTCCAGGCCGTCGTGGTCAACCGCGGCTCCGACCTGCACGTGAAGGTCGGCAGCCCGCCGCG
GATCCGCATCTCGGGGTCGCTGGTCCCGCTGCAGGTGGACCCGCTGACCGCCGAGCAGGTCGAGGAGCTGGTCCGCGAGA
CCATGCCCGCGGACGTCGAGGCGCACTTCAGCGCCACCAACGAGGCCGACTACGCGCTCGCCGTCCCCGGCATCGGCCGG
TTCCGCGTCAACGCGTTCCGGTCACGAGGGGCGGTCGGGTGCGTGTTGCGCCTGGTGGGCGACGAGCCGCGCTCGCTGGC
GGACCTGGCCCTGCCCGAGGTGATCCACCGCCTCGCCCTGGAGCCGCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGCT
CGGGCAAGACCACGACCCTGGCGGCGATGGTCGACGCCATCAACGAGGCCCGGCCCGTGCACATCCTCACCATCGAGGAC
CCGATCGAGGTGATCCACCGCGACAAGCTCGCGGCGGTCAACCAGCGCGAGCTCGGCACCGACACCGCCGACTGGGCGGT
GGCGCTGCGGGCCGCGATGCGGCAGGACCCGGACGTGATCCTGATCGGCGAGATGCGCGACCCCGACACCGTCCGGGCCG
CGCTCGCGGCCGCCGAGACCGGGCACTTCGTGATGTCGACGCTGCACACCACGGACGCCAAGGAGACGGTCAACCGGATC
ATCGACTTCTTCCCCCCGCATGAGCAGAAGCAGGTACGCCTCGGGCTCGCCGGCTCGCTGCGCGGCATCGTCTGCCAGCG
GCTGGTGCCGCGCGCCGACGGCCAGGGCCGGGTCGTCGCGATGGAGGTCGCCGTGAACACGCCGCGGCTCGCCGAGGCGG
TCGCCGACCCGGACCGCACCGACACGATCCCGGACATCGTCTCCGACGGCGCCTACTCCGGCATGCAGACCTTCGACCAG
CACCTGGTCCGGTTGGTGCTCGACGGCTCGGTCTCGGTCCCCGACGCGAAGCTGGTCAGCTCCAACACCCACGACTTCTC
GGTGATGCTGAAGCGCGCCGGGGTCGATCCGGCCCTGGTCGACGCGGAGCCGCCCCGATGA

Upstream 100 bases:

>100_bases
GGGAAACCGCCCGCCGATCCCGCCGCACCTGGACCGACGACAACGCCGACGTGGTACACAAGGGGCCGTGAGCAGCCCCC
GGGATCCGAGCGCGACCCCG

Downstream 100 bases:

>100_bases
GCGGCACCCGCCGCCGCCCGGTGGTCGACGCGATGGCGTCCGAGCAGCTCGCCGGGCTCGACCTGCAGGGCCTGCGCTCC
TACCGCCACCGCCTCGAGGC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRIAPFLQAVVVNRGSDLHVKVGSPPRIRISGSLVPLQVDPLTAEQVEELVRETMPADVEAHFSATNEADYALAVPGIGR
FRVNAFRSRGAVGCVLRLVGDEPRSLADLALPEVIHRLALEPRGLVLVTGPTGSGKTTTLAAMVDAINEARPVHILTIED
PIEVIHRDKLAAVNQRELGTDTADWAVALRAAMRQDPDVILIGEMRDPDTVRAALAAAETGHFVMSTLHTTDAKETVNRI
IDFFPPHEQKQVRLGLAGSLRGIVCQRLVPRADGQGRVVAMEVAVNTPRLAEAVADPDRTDTIPDIVSDGAYSGMQTFDQ
HLVRLVLDGSVSVPDAKLVSSNTHDFSVMLKRAGVDPALVDAEPPR

Sequences:

>Translated_366_residues
MRIAPFLQAVVVNRGSDLHVKVGSPPRIRISGSLVPLQVDPLTAEQVEELVRETMPADVEAHFSATNEADYALAVPGIGR
FRVNAFRSRGAVGCVLRLVGDEPRSLADLALPEVIHRLALEPRGLVLVTGPTGSGKTTTLAAMVDAINEARPVHILTIED
PIEVIHRDKLAAVNQRELGTDTADWAVALRAAMRQDPDVILIGEMRDPDTVRAALAAAETGHFVMSTLHTTDAKETVNRI
IDFFPPHEQKQVRLGLAGSLRGIVCQRLVPRADGQGRVVAMEVAVNTPRLAEAVADPDRTDTIPDIVSDGAYSGMQTFDQ
HLVRLVLDGSVSVPDAKLVSSNTHDFSVMLKRAGVDPALVDAEPPR
>Mature_366_residues
MRIAPFLQAVVVNRGSDLHVKVGSPPRIRISGSLVPLQVDPLTAEQVEELVRETMPADVEAHFSATNEADYALAVPGIGR
FRVNAFRSRGAVGCVLRLVGDEPRSLADLALPEVIHRLALEPRGLVLVTGPTGSGKTTTLAAMVDAINEARPVHILTIED
PIEVIHRDKLAAVNQRELGTDTADWAVALRAAMRQDPDVILIGEMRDPDTVRAALAAAETGHFVMSTLHTTDAKETVNRI
IDFFPPHEQKQVRLGLAGSLRGIVCQRLVPRADGQGRVVAMEVAVNTPRLAEAVADPDRTDTIPDIVSDGAYSGMQTFDQ
HLVRLVLDGSVSVPDAKLVSSNTHDFSVMLKRAGVDPALVDAEPPR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=317, Percent_Identity=41.3249211356467, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=35.2313167259786, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789723, Length=157, Percent_Identity=44.5859872611465, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39400; Mature: 39400

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAPFLQAVVVNRGSDLHVKVGSPPRIRISGSLVPLQVDPLTAEQVEELVRETMPADVE
CCCHHHHHHHHHCCCCEEEEEECCCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCCCCE
AHFSATNEADYALAVPGIGRFRVNAFRSRGAVGCVLRLVGDEPRSLADLALPEVIHRLAL
EEECCCCCCCEEEECCCCCHHHHHHHHCCCHHEEHHEECCCCCHHHHHHHHHHHHHHHHC
EPRGLVLVTGPTGSGKTTTLAAMVDAINEARPVHILTIEDPIEVIHRDKLAAVNQRELGT
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCHHHCCC
DTADWAVALRAAMRQDPDVILIGEMRDPDTVRAALAAAETGHFVMSTLHTTDAKETVNRI
CHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHEEHHHHCCCHHHHHHHH
IDFFPPHEQKQVRLGLAGSLRGIVCQRLVPRADGQGRVVAMEVAVNTPRLAEAVADPDRT
HHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHCCCCCC
DTIPDIVSDGAYSGMQTFDQHLVRLVLDGSVSVPDAKLVSSNTHDFSVMLKRAGVDPALV
CCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCEEC
DAEPPR
CCCCCC
>Mature Secondary Structure
MRIAPFLQAVVVNRGSDLHVKVGSPPRIRISGSLVPLQVDPLTAEQVEELVRETMPADVE
CCCHHHHHHHHHCCCCEEEEEECCCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCCCCE
AHFSATNEADYALAVPGIGRFRVNAFRSRGAVGCVLRLVGDEPRSLADLALPEVIHRLAL
EEECCCCCCCEEEECCCCCHHHHHHHHCCCHHEEHHEECCCCCHHHHHHHHHHHHHHHHC
EPRGLVLVTGPTGSGKTTTLAAMVDAINEARPVHILTIEDPIEVIHRDKLAAVNQRELGT
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCHHHCCC
DTADWAVALRAAMRQDPDVILIGEMRDPDTVRAALAAAETGHFVMSTLHTTDAKETVNRI
CHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHEEHHHHCCCHHHHHHHH
IDFFPPHEQKQVRLGLAGSLRGIVCQRLVPRADGQGRVVAMEVAVNTPRLAEAVADPDRT
HHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHCCCCCC
DTIPDIVSDGAYSGMQTFDQHLVRLVLDGSVSVPDAKLVSSNTHDFSVMLKRAGVDPALV
CCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCEEC
DAEPPR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]