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The map label for this gene is pinE [H]

Identifier: 119715327

GI number: 119715327

Start: 1148506

End: 1149189

Strand: Reverse

Name: pinE [H]

Synonym: Noca_1088

Alternate gene names: 119715327

Gene position: 1149189-1148506 (Counterclockwise)

Preceding gene: 119715328

Following gene: 119715326

Centisome position: 23.05

GC content: 65.06

Gene sequence:

>684_bases
ATGGGAAAACGCCGCCGGAGTGATGCTGTCATCGGCTACCTGCGGGTGAGCACAGAGGAGCAGGCGGTCTCTGGCCTCGG
CTTGGCGGACCAGAGGTCGGCGATTGAGGCGGCTTCCGCCGCCCGGAACTGGCCTGCTGTCACCTACATCTCAGACGAAG
GCCACTCGGCCCGCAGTCTTGGGCGCCCGGGAATCTCGGCGGTGCTGACAGCCCTCGCGCGGGGGGAAGCATCCGTGCTC
GTAGTCGCGAAGCTTGACCGCCTCTCCCGGTCGCTGCTGGACTTCGCCATGCTCATGGAACGAGCGCGTCGAGAGGGTTG
GGAACTGGTAGTCCTCGATCTGGCGATCGACACGACAACCCCGAGCGGAGCGCTCATGGCAAACGTCATGGCGTCGTTCG
CCGAGTACGAGCGCCAACTCATTGGCACCAGAACAAGCGCGGCCCTCCAGCAACTGAAGATGCAAGGAGTCCGCTTGGGC
CGGCCCCGCAAGACGTCCATCGAAACCCTGAGCCGGATAGCACGCCAACGGGACGCGGGCGAGACGCTAGCAGCGATCGC
TGGCGGGCTTAACCGGGACGGAGTCCCAACCACGCGCGGGGGAGCCAAGTGGTACCCGTCAACGGTTCGCGCCGCCTTGG
AGTCGATGAAGCTGGACGATGCTCTAAGTACGACCACAAGGTGA

Upstream 100 bases:

>100_bases
AGTTCCTAAGACCGTTCTGGATGCGGCGTGCACGGTGCAGAGCGGCTCGGAGTTCGGCGAGACGCATACACAAAACGACC
GTTTCATGTATTCTGAGTCC

Downstream 100 bases:

>100_bases
GGCCGACACCAACCGAACGGGAATCATGTTCACTGTGACGGGCACGATCGACTCAATCCTCTACCGTGTTGGCATCCATC
CCGCCGCACTAGAAGGCGAA

Product: resolvase domain-containing protein-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MGKRRRSDAVIGYLRVSTEEQAVSGLGLADQRSAIEAASAARNWPAVTYISDEGHSARSLGRPGISAVLTALARGEASVL
VVAKLDRLSRSLLDFAMLMERARREGWELVVLDLAIDTTTPSGALMANVMASFAEYERQLIGTRTSAALQQLKMQGVRLG
RPRKTSIETLSRIARQRDAGETLAAIAGGLNRDGVPTTRGGAKWYPSTVRAALESMKLDDALSTTTR

Sequences:

>Translated_227_residues
MGKRRRSDAVIGYLRVSTEEQAVSGLGLADQRSAIEAASAARNWPAVTYISDEGHSARSLGRPGISAVLTALARGEASVL
VVAKLDRLSRSLLDFAMLMERARREGWELVVLDLAIDTTTPSGALMANVMASFAEYERQLIGTRTSAALQQLKMQGVRLG
RPRKTSIETLSRIARQRDAGETLAAIAGGLNRDGVPTTRGGAKWYPSTVRAALESMKLDDALSTTTR
>Mature_226_residues
GKRRRSDAVIGYLRVSTEEQAVSGLGLADQRSAIEAASAARNWPAVTYISDEGHSARSLGRPGISAVLTALARGEASVLV
VAKLDRLSRSLLDFAMLMERARREGWELVVLDLAIDTTTPSGALMANVMASFAEYERQLIGTRTSAALQQLKMQGVRLGR
PRKTSIETLSRIARQRDAGETLAAIAGGLNRDGVPTTRGGAKWYPSTVRAALESMKLDDALSTTTR

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=158, Percent_Identity=37.9746835443038, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787827, Length=179, Percent_Identity=30.7262569832402, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787638, Length=179, Percent_Identity=30.7262569832402, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 24414; Mature: 24283

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRRRSDAVIGYLRVSTEEQAVSGLGLADQRSAIEAASAARNWPAVTYISDEGHSARSL
CCCCCCCCCEEEEEEECCHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHC
GRPGISAVLTALARGEASVLVVAKLDRLSRSLLDFAMLMERARREGWELVVLDLAIDTTT
CCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCC
PSGALMANVMASFAEYERQLIGTRTSAALQQLKMQGVRLGRPRKTSIETLSRIARQRDAG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
ETLAAIAGGLNRDGVPTTRGGAKWYPSTVRAALESMKLDDALSTTTR
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKRRRSDAVIGYLRVSTEEQAVSGLGLADQRSAIEAASAARNWPAVTYISDEGHSARSL
CCCCCCCCEEEEEEECCHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHC
GRPGISAVLTALARGEASVLVVAKLDRLSRSLLDFAMLMERARREGWELVVLDLAIDTTT
CCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCC
PSGALMANVMASFAEYERQLIGTRTSAALQQLKMQGVRLGRPRKTSIETLSRIARQRDAG
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
ETLAAIAGGLNRDGVPTTRGGAKWYPSTVRAALESMKLDDALSTTTR
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]