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The map label for this gene is thcD [H]

Identifier: 119714933

GI number: 119714933

Start: 724797

End: 726050

Strand: Direct

Name: thcD [H]

Synonym: Noca_0686

Alternate gene names: 119714933

Gene position: 724797-726050 (Clockwise)

Preceding gene: 119714932

Following gene: 119714936

Centisome position: 14.54

GC content: 72.09

Gene sequence:

>1254_bases
ATGAGTGGGCAGCGAATGAGCAAGCAGATCCCCGATCGGGTGGTGGTCGTCGGCTCCTCGGTCGGTGGGACCCGGGCTGC
GCAGGGCCTGCGGGCAGCGGGATTCACCGGGCAGGTCGTCATCGTCGGTGCCGAGAAGGTGGCGCCGTACGACAAGCCGC
CGCTCTCCAAGCAGCTGCTCTCCGGCGAGTGGAGCGTGGAGGACATCGCGCTGGCCTCGGTCGAGGAGCTGGGTGGCGCA
GGCATCGAGCTGCGCCTCGGTGTGGCCGCGGGCGGGGTCGACGTGGCCAACCGCACGTTGACGCTCGTCGACGGCACGAC
GCTCGGCTACGACGCGCTGGTCATCGCGACCGGGGTCCGGCCACGCACCCTCTCGGCCCCGCGGACCGTGGCCCTGCACA
CCGTGCGCGAGTTGGCCGACGTCAGCGCCCTCCGCGGGGCGCTGGACTCCGGCCATGGTCTGGTCGTGGTCGGCGGCGGC
TTCATCGGGGCCGAGATCGCTTCCACCGCCGCGGGTCTGGGCGTCCCTGTGTCGATCGTCGAAGCCTTGCCGGCGCCGTT
CTCGCGCGTGCTCGGAGATGAGGTCGGCGGCCTCCTGGCCCAGCTCCACGCCGAGAACGGGGTCGAGGTCGTGTCGGGCA
CGGGCGTCGTTGCACTGCGCTGTGGCCCGACCGGGCTCACCGAGGTCGAGCTGGGTGACGGGAGGGTGTTGTCGGCGGGT
GCGGTGGCCGTGGGGATCGGCTGCGTGCCGAACACCGATTGGCTGGCTGGCTCCGGGATCCCGCTCGCGGACGGCGTCGC
GACCGACCAGTTCTCGCGGGTCCGCGAGGTTCCGGGTGTCTACGCGATCGGGGATGTGGCGAACTGGCATGACGTGCGTA
CGGGAACGTCCCGGCGCGTGGAGCACTGGACGCACGCCTTCCAGCAGGCCCAGCTGGTGGCCCACAACATCGTGCACCCC
GATGACCTCCAAGCCCAGACACGGGCGCCGTACTTCTGGTCCGACCAGCACGGCGTCAAGATCCAGATGGTCGGGCGCCG
CGGGCGCGAGGACGCAGTCGACGTCATGTGGCGTGACACCTCGGACGGGCAGCGCCCGGTGGCGCTCTACTCCGACCGGG
GGCGGTTCACCGGCGGGGTCACCTTCGGCGCACCGCAGCTGAGTGCGCGGCTGCGACGCGCCTGGGAGGACGGCCTCGCG
CTGACGGACGCATGCGCCCTCGTCGCAGGACTGCCCGCCCCCGCTCGCGCCTGA

Upstream 100 bases:

>100_bases
CCCGGCAACGTCTTGCACCGGGAGCCGACGACGCCCGTGGGCGGCGACGACCCTGCGGCCGGAACCCGTGGCCAGGCATG
AAGCGGCAGAGCGGCAAGGA

Downstream 100 bases:

>100_bases
CCCGAGTGCCGGCCCGGCTCAGCCGTAGGCCCCCCGCCAGCCCAGCGCTTCCGAGATCCGCCCGGCGGTCTCCGACAGCG
TCTTCAGCTGGACTGCCTGC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSGQRMSKQIPDRVVVVGSSVGGTRAAQGLRAAGFTGQVVIVGAEKVAPYDKPPLSKQLLSGEWSVEDIALASVEELGGA
GIELRLGVAAGGVDVANRTLTLVDGTTLGYDALVIATGVRPRTLSAPRTVALHTVRELADVSALRGALDSGHGLVVVGGG
FIGAEIASTAAGLGVPVSIVEALPAPFSRVLGDEVGGLLAQLHAENGVEVVSGTGVVALRCGPTGLTEVELGDGRVLSAG
AVAVGIGCVPNTDWLAGSGIPLADGVATDQFSRVREVPGVYAIGDVANWHDVRTGTSRRVEHWTHAFQQAQLVAHNIVHP
DDLQAQTRAPYFWSDQHGVKIQMVGRRGREDAVDVMWRDTSDGQRPVALYSDRGRFTGGVTFGAPQLSARLRRAWEDGLA
LTDACALVAGLPAPARA

Sequences:

>Translated_417_residues
MSGQRMSKQIPDRVVVVGSSVGGTRAAQGLRAAGFTGQVVIVGAEKVAPYDKPPLSKQLLSGEWSVEDIALASVEELGGA
GIELRLGVAAGGVDVANRTLTLVDGTTLGYDALVIATGVRPRTLSAPRTVALHTVRELADVSALRGALDSGHGLVVVGGG
FIGAEIASTAAGLGVPVSIVEALPAPFSRVLGDEVGGLLAQLHAENGVEVVSGTGVVALRCGPTGLTEVELGDGRVLSAG
AVAVGIGCVPNTDWLAGSGIPLADGVATDQFSRVREVPGVYAIGDVANWHDVRTGTSRRVEHWTHAFQQAQLVAHNIVHP
DDLQAQTRAPYFWSDQHGVKIQMVGRRGREDAVDVMWRDTSDGQRPVALYSDRGRFTGGVTFGAPQLSARLRRAWEDGLA
LTDACALVAGLPAPARA
>Mature_416_residues
SGQRMSKQIPDRVVVVGSSVGGTRAAQGLRAAGFTGQVVIVGAEKVAPYDKPPLSKQLLSGEWSVEDIALASVEELGGAG
IELRLGVAAGGVDVANRTLTLVDGTTLGYDALVIATGVRPRTLSAPRTVALHTVRELADVSALRGALDSGHGLVVVGGGF
IGAEIASTAAGLGVPVSIVEALPAPFSRVLGDEVGGLLAQLHAENGVEVVSGTGVVALRCGPTGLTEVELGDGRVLSAGA
VAVGIGCVPNTDWLAGSGIPLADGVATDQFSRVREVPGVYAIGDVANWHDVRTGTSRRVEHWTHAFQQAQLVAHNIVHPD
DLQAQTRAPYFWSDQHGVKIQMVGRRGREDAVDVMWRDTSDGQRPVALYSDRGRFTGGVTFGAPQLSARLRRAWEDGLAL
TDACALVAGLPAPARA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=336, Percent_Identity=32.4404761904762, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI226437568, Length=336, Percent_Identity=32.4404761904762, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI21389617, Length=336, Percent_Identity=32.4404761904762, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4757732, Length=350, Percent_Identity=23.4285714285714, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI22202629, Length=350, Percent_Identity=23.4285714285714, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788892, Length=373, Percent_Identity=28.4182305630027, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1789065, Length=275, Percent_Identity=22.5454545454545, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=346, Percent_Identity=30.3468208092486, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI32564386, Length=340, Percent_Identity=27.6470588235294, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585130, Length=327, Percent_Identity=28.1345565749235, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24639257, Length=359, Percent_Identity=28.4122562674095, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281359715, Length=359, Percent_Identity=28.4122562674095, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281359713, Length=359, Percent_Identity=28.4122562674095, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639250, Length=359, Percent_Identity=28.4122562674095, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18543267, Length=359, Percent_Identity=28.4122562674095, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24639252, Length=359, Percent_Identity=28.4122562674095, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24581020, Length=379, Percent_Identity=26.9129287598945, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI28573993, Length=379, Percent_Identity=26.9129287598945, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43162; Mature: 43031

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQRMSKQIPDRVVVVGSSVGGTRAAQGLRAAGFTGQVVIVGAEKVAPYDKPPLSKQLL
CCCCHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCCCCEEEEECHHCCCCCCCCHHHHHH
SGEWSVEDIALASVEELGGAGIELRLGVAAGGVDVANRTLTLVDGTTLGYDALVIATGVR
CCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCC
PRTLSAPRTVALHTVRELADVSALRGALDSGHGLVVVGGGFIGAEIASTAAGLGVPVSIV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHH
EALPAPFSRVLGDEVGGLLAQLHAENGVEVVSGTGVVALRCGPTGLTEVELGDGRVLSAG
HHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEECCCCEEEECC
AVAVGIGCVPNTDWLAGSGIPLADGVATDQFSRVREVPGVYAIGDVANWHDVRTGTSRRV
CEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHCCCCCHHHH
EHWTHAFQQAQLVAHNIVHPDDLQAQTRAPYFWSDQHGVKIQMVGRRGREDAVDVMWRDT
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEECCCCCEEEEEECCCCCCCCEEEEEECC
SDGQRPVALYSDRGRFTGGVTFGAPQLSARLRRAWEDGLALTDACALVAGLPAPARA
CCCCCCEEEEECCCCEECCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGQRMSKQIPDRVVVVGSSVGGTRAAQGLRAAGFTGQVVIVGAEKVAPYDKPPLSKQLL
CCCHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCCCCEEEEECHHCCCCCCCCHHHHHH
SGEWSVEDIALASVEELGGAGIELRLGVAAGGVDVANRTLTLVDGTTLGYDALVIATGVR
CCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEEECCC
PRTLSAPRTVALHTVRELADVSALRGALDSGHGLVVVGGGFIGAEIASTAAGLGVPVSIV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHH
EALPAPFSRVLGDEVGGLLAQLHAENGVEVVSGTGVVALRCGPTGLTEVELGDGRVLSAG
HHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEECCCCEEEECC
AVAVGIGCVPNTDWLAGSGIPLADGVATDQFSRVREVPGVYAIGDVANWHDVRTGTSRRV
CEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHCCCCCHHHH
EHWTHAFQQAQLVAHNIVHPDDLQAQTRAPYFWSDQHGVKIQMVGRRGREDAVDVMWRDT
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEECCCCCEEEEEECCCCCCCCEEEEEECC
SDGQRPVALYSDRGRFTGGVTFGAPQLSARLRRAWEDGLALTDACALVAGLPAPARA
CCCCCCEEEEECCCCEECCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]