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The map label for this gene is hcaB [H]

Identifier: 119714936

GI number: 119714936

Start: 728112

End: 728921

Strand: Direct

Name: hcaB [H]

Synonym: Noca_0689

Alternate gene names: 119714936

Gene position: 728112-728921 (Clockwise)

Preceding gene: 119714933

Following gene: 119714937

Centisome position: 14.6

GC content: 71.73

Gene sequence:

>810_bases
GTGTCCCAGGCCGGTGACCACGAGCTCGCCGGACGGGCAATGCTCCTGACCGGGGCAGCGACCGGCATCGGAGCTGCGCT
GTCGGCGGGTCTGCGCTCGGTGGGCTGTCACGTCGTCGAGCTCGACCGAGTTCCGCTCGCCGCCGCCGACGACCTGATCG
CCCTTCCCGGCCGTCGGCACCGCGTCGTCGGCGACGTGACCGATCCGGAGGCCAATCTCGAGGCCGTGCGGCTGGCCCTT
CGCGAGTTCGGGGCCCTCGACGGCTTCATCGGGAACGCCGGTGTGCACGACGGCGGCGTGCACGTCCGCGATGCCGACCC
GGCCACCCTGCGTGACCTCACCCGCACGGTCCTCGACGTCAACGTGGTCGGTTACGTCGTGGGTGCCTGCGCGGCCGCCG
AGGCGGTGACGCAAGCGGGTGGCGTCATGCTCTTCACGCTGTCCGACGCGTCGTACGTCGTGTCCGGCAACGGCGCCGGC
ATCGCCTATGCCGCCTCCAAGCACGCCGCGCTCGGTGTGGTGCGGTCGCTCGCGGCCAAGCTGGCGCCCGCGGTACGCGT
GAACGCGGTCGCTCCGGGCGGGGTGGTGACGCCGCTGAGCCGGGTGGCGGCGGACGGCGAGGCCACGGGGCGGCAGCAGG
TCTACGAGAACGTCGAGGACTCGGTGGCCGGCATCCGTGCGCTCAATCCACTGCGCACCATCCTCACGCCCGAGGAACTC
GTTCCTCACTACCTCTACCTGTTGACCAGTCAGAGCACGGGTCTGACCGGTCACGTCGTCCGCCCGGACGGCGGCCTCTC
GATCGCCTGA

Upstream 100 bases:

>100_bases
GGGCCGGTCCGAGCCGGACGACGCTTGACAGCGTGTCAGTCTCGATGCGAGCATAATGCTCGCATTATGAACAACGCATC
CAAATTTACGAGGAGGGTCC

Downstream 100 bases:

>100_bases
CCGGCGGTCGACCGATCCACCCCGCACCCGACTACTCCGGCGCGCTCGCGCCCCCACCGAAGGAGACCCTCGTGGCCAGC
TACGAGAACGCCACCGACGC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase; CI-dihydrodiol dehydrogenase; Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase; Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase; PP-dihydrodiol dehydrogenase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSQAGDHELAGRAMLLTGAATGIGAALSAGLRSVGCHVVELDRVPLAAADDLIALPGRRHRVVGDVTDPEANLEAVRLAL
REFGALDGFIGNAGVHDGGVHVRDADPATLRDLTRTVLDVNVVGYVVGACAAAEAVTQAGGVMLFTLSDASYVVSGNGAG
IAYAASKHAALGVVRSLAAKLAPAVRVNAVAPGGVVTPLSRVAADGEATGRQQVYENVEDSVAGIRALNPLRTILTPEEL
VPHYLYLLTSQSTGLTGHVVRPDGGLSIA

Sequences:

>Translated_269_residues
MSQAGDHELAGRAMLLTGAATGIGAALSAGLRSVGCHVVELDRVPLAAADDLIALPGRRHRVVGDVTDPEANLEAVRLAL
REFGALDGFIGNAGVHDGGVHVRDADPATLRDLTRTVLDVNVVGYVVGACAAAEAVTQAGGVMLFTLSDASYVVSGNGAG
IAYAASKHAALGVVRSLAAKLAPAVRVNAVAPGGVVTPLSRVAADGEATGRQQVYENVEDSVAGIRALNPLRTILTPEEL
VPHYLYLLTSQSTGLTGHVVRPDGGLSIA
>Mature_268_residues
SQAGDHELAGRAMLLTGAATGIGAALSAGLRSVGCHVVELDRVPLAAADDLIALPGRRHRVVGDVTDPEANLEAVRLALR
EFGALDGFIGNAGVHDGGVHVRDADPATLRDLTRTVLDVNVVGYVVGACAAAEAVTQAGGVMLFTLSDASYVVSGNGAGI
AYAASKHAALGVVRSLAAKLAPAVRVNAVAPGGVVTPLSRVAADGEATGRQQVYENVEDSVAGIRALNPLRTILTPEELV
PHYLYLLTSQSTGLTGHVVRPDGGLSIA

Specific function: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3- dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7706318, Length=221, Percent_Identity=27.6018099547511, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788891, Length=208, Percent_Identity=36.0576923076923, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082100, Length=268, Percent_Identity=30.9701492537313, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789208, Length=264, Percent_Identity=26.1363636363636, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787335, Length=262, Percent_Identity=26.3358778625954, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.-

Molecular weight: Translated: 27344; Mature: 27213

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAGDHELAGRAMLLTGAATGIGAALSAGLRSVGCHVVELDRVPLAAADDLIALPGRRH
CCCCCCCHHCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCC
RVVGDVTDPEANLEAVRLALREFGALDGFIGNAGVHDGGVHVRDADPATLRDLTRTVLDV
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
NVVGYVVGACAAAEAVTQAGGVMLFTLSDASYVVSGNGAGIAYAASKHAALGVVRSLAAK
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCEEEEECCHHHHHHHHHHHHHH
LAPAVRVNAVAPGGVVTPLSRVAADGEATGRQQVYENVEDSVAGIRALNPLRTILTPEEL
HCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VPHYLYLLTSQSTGLTGHVVRPDGGLSIA
HHHEEEEEECCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
SQAGDHELAGRAMLLTGAATGIGAALSAGLRSVGCHVVELDRVPLAAADDLIALPGRRH
CCCCCCHHCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEECCCCCC
RVVGDVTDPEANLEAVRLALREFGALDGFIGNAGVHDGGVHVRDADPATLRDLTRTVLDV
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
NVVGYVVGACAAAEAVTQAGGVMLFTLSDASYVVSGNGAGIAYAASKHAALGVVRSLAAK
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCEEEEECCHHHHHHHHHHHHHH
LAPAVRVNAVAPGGVVTPLSRVAADGEATGRQQVYENVEDSVAGIRALNPLRTILTPEEL
HCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VPHYLYLLTSQSTGLTGHVVRPDGGLSIA
HHHEEEEEECCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]