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The map label for this gene is nuoB

Identifier: 119714785

GI number: 119714785

Start: 551532

End: 552086

Strand: Direct

Name: nuoB

Synonym: Noca_0521

Alternate gene names: 119714785

Gene position: 551532-552086 (Clockwise)

Preceding gene: 119714784

Following gene: 119714786

Centisome position: 11.06

GC content: 68.47

Gene sequence:

>555_bases
ATGGGAATTGAGGAGAAGCTCCCGAGCGGGGTGCTGCTCTCGACCGTCGAGGGCCTGGCGGGCTACATGCGCAAGGCCTC
GTTCTGGCCGGCGTCCTTCGGCCTCGCCTGCTGCGCGATCGAGATGATGACCAGTGGCGGCCCCAAGCACGACCTGGGCC
GCTTCGGCATGGAGGTGTTCCGGGCCAGCCCCCGCCAGGCCGACGTGATGATCGTCGCCGGCCGGGTGAGCCAGAAGATG
GCCCCGGTGCTGCGCCAGATCTACGACCAGATGCCCGAGCCCAAGTGGGTGCTGGCGATGGGTGTGTGCGCCAGCAGCGG
CGGCATGTTCAACAACTACGCGGTCGTGCAGGGCGTCGACCACGTCGTCCCGGTCGACATGTACCTCCCCGGTTGCCCAC
CCCGGCCGCAGATGCTCATCGACGCGATCTTGAAGCTGCACGACAAGGTCCAGCACACCAAGATGGGCGCCCACCGCGCG
GCCGAGATCGAGGAGCTCGAGACGGCGGCGCTGCGCGCGCTCCCCACCTCCGAGATGAAGGGCCAGCTGCGGTGA

Upstream 100 bases:

>100_bases
GGGCTGGTCGAGATGGTCTTGTTCATCGCCACGGTCTTCGTCGCCTATGCCTACGTGTGGCGCCGCGGCGGGCTCGATTG
GGACTGAGAGGAACCAACTG

Downstream 100 bases:

>100_bases
GCGACGACTCCAGCGACGTGAAGCCGGGCCCGAAGGGACCCGAGCAGCCGGCCGTCGAGCAATCCCCGGAGAACGTCCCG
GCGCCCACGGGAGAGGTCCA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MGIEEKLPSGVLLSTVEGLAGYMRKASFWPASFGLACCAIEMMTSGGPKHDLGRFGMEVFRASPRQADVMIVAGRVSQKM
APVLRQIYDQMPEPKWVLAMGVCASSGGMFNNYAVVQGVDHVVPVDMYLPGCPPRPQMLIDAILKLHDKVQHTKMGAHRA
AEIEELETAALRALPTSEMKGQLR

Sequences:

>Translated_184_residues
MGIEEKLPSGVLLSTVEGLAGYMRKASFWPASFGLACCAIEMMTSGGPKHDLGRFGMEVFRASPRQADVMIVAGRVSQKM
APVLRQIYDQMPEPKWVLAMGVCASSGGMFNNYAVVQGVDHVVPVDMYLPGCPPRPQMLIDAILKLHDKVQHTKMGAHRA
AEIEELETAALRALPTSEMKGQLR
>Mature_183_residues
GIEEKLPSGVLLSTVEGLAGYMRKASFWPASFGLACCAIEMMTSGGPKHDLGRFGMEVFRASPRQADVMIVAGRVSQKMA
PVLRQIYDQMPEPKWVLAMGVCASSGGMFNNYAVVQGVDHVVPVDMYLPGCPPRPQMLIDAILKLHDKVQHTKMGAHRAA
EIEELETAALRALPTSEMKGQLR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=144, Percent_Identity=52.0833333333333, Blast_Score=174, Evalue=4e-44,
Organism=Escherichia coli, GI1788624, Length=148, Percent_Identity=52.027027027027, Blast_Score=183, Evalue=5e-48,
Organism=Escherichia coli, GI1789074, Length=105, Percent_Identity=42.8571428571429, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788834, Length=109, Percent_Identity=39.4495412844037, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=143, Percent_Identity=50.3496503496504, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI18859983, Length=143, Percent_Identity=53.8461538461538, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24642371, Length=143, Percent_Identity=53.8461538461538, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24651058, Length=155, Percent_Identity=50.9677419354839, Blast_Score=174, Evalue=3e-44,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_NOCSJ (A1SE28)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_921750.1
- ProteinModelPortal:   A1SE28
- SMR:   A1SE28
- STRING:   A1SE28
- GeneID:   4596441
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_0521
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   EAKWVIS
- PhylomeDB:   A1SE28
- ProtClustDB:   PRK06411
- BioCyc:   NSP35761:NOCA_0521-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20057; Mature: 19926

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
7.6 %Met     (Translated Protein)
9.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
7.1 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIEEKLPSGVLLSTVEGLAGYMRKASFWPASFGLACCAIEMMTSGGPKHDLGRFGMEVF
CCCCHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RASPRQADVMIVAGRVSQKMAPVLRQIYDQMPEPKWVLAMGVCASSGGMFNNYAVVQGVD
HCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCHHHHHCCC
HVVPVDMYLPGCPPRPQMLIDAILKLHDKVQHTKMGAHRAAEIEELETAALRALPTSEMK
CEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GQLR
CCCC
>Mature Secondary Structure 
GIEEKLPSGVLLSTVEGLAGYMRKASFWPASFGLACCAIEMMTSGGPKHDLGRFGMEVF
CCCHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RASPRQADVMIVAGRVSQKMAPVLRQIYDQMPEPKWVLAMGVCASSGGMFNNYAVVQGVD
HCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCHHHHHCCC
HVVPVDMYLPGCPPRPQMLIDAILKLHDKVQHTKMGAHRAAEIEELETAALRALPTSEMK
CEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
GQLR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA