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The map label for this gene is tilS [H]

Identifier: 119714705

GI number: 119714705

Start: 472519

End: 473484

Strand: Direct

Name: tilS [H]

Synonym: Noca_0441

Alternate gene names: 119714705

Gene position: 472519-473484 (Clockwise)

Preceding gene: 119714704

Following gene: 119714706

Centisome position: 9.48

GC content: 76.92

Gene sequence:

>966_bases
ATGAGCCTCCACCCCGCGATCGCCGCGGTGCGCCTCGCGGTACGCCGTGCCCTCGCCCACGTCGACCCGGGTCGGACCGT
GGTCGTCGCGTGCTCCGGGGGCGCCGACTCGCTCGCGTTGCTCGCCGCGACGGTGTTCGAGGGCCGACGCGCCGGGTGGC
GGGTCGTGGGCGCGACGGTCGACCACGGGCTCCAGCCCGGCTCGGCCGACCACGCCGCGCACGTCGTACGCCAGATGGCC
GCCCTCGGCGCCGACGAGACCGTCGCCGCGACCGTGCACGTCGAGGCGGCCGGCCAGGGCCCGGAGGCGGCGGCCCGCCA
GGCCCGCTACGCCGTCCTCGACCAGCTCGCCGAGCGCTTCGACGCCGACGCCGTGCTGCTCGGCCACACCCGCGACGACC
AGGCCGAGACGGTGCTGCTCGGCCTGGCCAGGGGCTCCGGGGGGCGGTCGCTGGCCGGGATGCGACGCTCCTTCGACCGC
TTCGTCCGCCCGCTCCTCGACCTCTCCCGCGCCGACACCGCCACCGCCTGCCAGGTCGCGGGGATCGAGTTCTGGAGCGA
CCCCCACAACGAGGACCCCGCATTCACCCGGGTGCGGGTACGCCGCAGCGTGCTGCCGGTCCTCGAGGACCAGCTCGGTC
CGGGGGTGGCCGAGGCGCTGGCCCGGACCGCCGACCAGCTGCGCGCGGACATGGACCTGCTCGACGACGCCGCCGAGGCG
GCGTACCAGCGCCTGCGCGACCTTCCTGTCGACGGGCTGCTCGCCGAGCCCGGCCCGATCCGGCGGCGGGTGCTGCGGCT
GGCCGCGCTGGCCGCCGGCGCCCCCGCCTCCGAGCTCTTCCACGAGCACGTGCTCGCGATGGACGCGCTGCTCACCCACT
GGCACGGCCAGAAATGGGTGGACCTCCCCGGGAAGGTGCGCTGCCGACGAGCCGACGGCCGGCTGCGCCTCGGCCCGGCT
GGATGA

Upstream 100 bases:

>100_bases
CGGTCTGGGAGCGGCCCGAGAACCTGCCGTCGAAGGCCGAGATCGCGGACCCACGAGCCTGGATCAGCCGGGTGCTGTGA
CCGTCCCACGGGCCTGACCC

Downstream 100 bases:

>100_bases
GAAGTCTCAGGTTCCGCCTGCTCCGACCGATGTTCCGCGTCAGAATCGCAATCGACGTCCGTCTGGTCAGCGTGCCGACT
GAAACGAGGAACTCCATGTC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSLHPAIAAVRLAVRRALAHVDPGRTVVVACSGGADSLALLAATVFEGRRAGWRVVGATVDHGLQPGSADHAAHVVRQMA
ALGADETVAATVHVEAAGQGPEAAARQARYAVLDQLAERFDADAVLLGHTRDDQAETVLLGLARGSGGRSLAGMRRSFDR
FVRPLLDLSRADTATACQVAGIEFWSDPHNEDPAFTRVRVRRSVLPVLEDQLGPGVAEALARTADQLRADMDLLDDAAEA
AYQRLRDLPVDGLLAEPGPIRRRVLRLAALAAGAPASELFHEHVLAMDALLTHWHGQKWVDLPGKVRCRRADGRLRLGPA
G

Sequences:

>Translated_321_residues
MSLHPAIAAVRLAVRRALAHVDPGRTVVVACSGGADSLALLAATVFEGRRAGWRVVGATVDHGLQPGSADHAAHVVRQMA
ALGADETVAATVHVEAAGQGPEAAARQARYAVLDQLAERFDADAVLLGHTRDDQAETVLLGLARGSGGRSLAGMRRSFDR
FVRPLLDLSRADTATACQVAGIEFWSDPHNEDPAFTRVRVRRSVLPVLEDQLGPGVAEALARTADQLRADMDLLDDAAEA
AYQRLRDLPVDGLLAEPGPIRRRVLRLAALAAGAPASELFHEHVLAMDALLTHWHGQKWVDLPGKVRCRRADGRLRLGPA
G
>Mature_320_residues
SLHPAIAAVRLAVRRALAHVDPGRTVVVACSGGADSLALLAATVFEGRRAGWRVVGATVDHGLQPGSADHAAHVVRQMAA
LGADETVAATVHVEAAGQGPEAAARQARYAVLDQLAERFDADAVLLGHTRDDQAETVLLGLARGSGGRSLAGMRRSFDRF
VRPLLDLSRADTATACQVAGIEFWSDPHNEDPAFTRVRVRRSVLPVLEDQLGPGVAEALARTADQLRADMDLLDDAAEAA
YQRLRDLPVDGLLAEPGPIRRRVLRLAALAAGAPASELFHEHVLAMDALLTHWHGQKWVDLPGKVRCRRADGRLRLGPAG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=208, Percent_Identity=32.2115384615385, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 34192; Mature: 34061

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHPAIAAVRLAVRRALAHVDPGRTVVVACSGGADSLALLAATVFEGRRAGWRVVGATV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEHH
DHGLQPGSADHAAHVVRQMAALGADETVAATVHVEAAGQGPEAAARQARYAVLDQLAERF
HCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
DADAVLLGHTRDDQAETVLLGLARGSGGRSLAGMRRSFDRFVRPLLDLSRADTATACQVA
CCCEEEEECCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GIEFWSDPHNEDPAFTRVRVRRSVLPVLEDQLGPGVAEALARTADQLRADMDLLDDAAEA
CCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYQRLRDLPVDGLLAEPGPIRRRVLRLAALAAGAPASELFHEHVLAMDALLTHWHGQKWV
HHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
DLPGKVRCRRADGRLRLGPAG
CCCCCEEEEECCCEEECCCCC
>Mature Secondary Structure 
SLHPAIAAVRLAVRRALAHVDPGRTVVVACSGGADSLALLAATVFEGRRAGWRVVGATV
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEHH
DHGLQPGSADHAAHVVRQMAALGADETVAATVHVEAAGQGPEAAARQARYAVLDQLAERF
HCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
DADAVLLGHTRDDQAETVLLGLARGSGGRSLAGMRRSFDRFVRPLLDLSRADTATACQVA
CCCEEEEECCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GIEFWSDPHNEDPAFTRVRVRRSVLPVLEDQLGPGVAEALARTADQLRADMDLLDDAAEA
CCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AYQRLRDLPVDGLLAEPGPIRRRVLRLAALAAGAPASELFHEHVLAMDALLTHWHGQKWV
HHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
DLPGKVRCRRADGRLRLGPAG
CCCCCEEEEECCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA