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The map label for this gene is hemK [H]

Identifier: 119714686

GI number: 119714686

Start: 453119

End: 454018

Strand: Reverse

Name: hemK [H]

Synonym: Noca_0422

Alternate gene names: 119714686

Gene position: 454018-453119 (Counterclockwise)

Preceding gene: 119714693

Following gene: 119714684

Centisome position: 9.11

GC content: 77.44

Gene sequence:

>900_bases
GTGCTGACGCTCGGCATGGCAGCATCATCGACCATGGCCGGTCCCGCTCCCGACGACGCCCTCGTCGCGACCTTGCGGGC
CGCCGGGTGCGTGTTCGCCGAGGACGAGGCTGCGCTGCTCGCCCGCGAGGCCGGGGACGAGGCCGCGCTGGCGGCGATGG
TCGCGCGCCGGGTCGCCGGCGAGCCGCTGGAGCAGGTGCTCGGGTTCGCGGAGTTCCTCGGGCTGCGCGTCGCGGTCGAG
CCCGGGGTGTTCGTGCCGCGGCTGCGCACCACGCTGCTGGCCCGCCGGGCGATCCGGGCGCTCGAGCCGGGCGACCTCGC
CGTCGACCTGTGCTGCGGCACCGGCGCCATCGGCGTGGCACTGCTCGCGGCGGTCCCGGGCGCCGAGGTGCACGCAGCGG
ACATCGACCCGGCCGCGGTGCGCTGCGCCCGACGGAACCTGCCGCCCGACCGGGTCCACGAGGGCGACCTGTACGACGCG
CTGCCGGGCGAGCTGCGCGGGCGGGTGGCGGTCGTGGTCGCAAACGCGCCGTACGTCCCGACCGGCGCCATCGCGACGAT
GCCGCCCGAGGCGCGCGACCACGAGCACCGGGTGGCGCTCGACGGCGGCGCGGACGGTCTCGAGGTCGCCCGGCGGGTGA
TCGCCGGGGCGCGGCCGTGGCTCGCCCCCGGTGGCCGGGTGCTCGTGGAGACGAGCGCCGCCCAGCGCGACCTGGCCGCC
GCCCACGTCGTCGCGGCGGGCCTCGACGTCACCGTCGCGTCGGCCGCGGACGTCGACGGCAACGTCGTGATCGGGCGGCT
CCACCCCGAGCGACTCAGCCCCGAGCGACTCAGCCCCGAGCAGCTCAGTCCTGGGCAGCTCAGTCCTGGGCAGCTCAGTC
CTGGGCGGCCCAGGTGCTGA

Upstream 100 bases:

>100_bases
CATCTGGTCGAGCCAGCCGGGGCGCTCGCCACGCCGGGCGGCCACCACCGCCAGGACGGCGACGAGCGCGGCGTACCCGA
CCACGATCCAGGTGATCTGG

Downstream 100 bases:

>100_bases
CCGTGTCGCGCAGCGGCACCGTGGGCCGGCCGAGCAGCTTCGCGAGGTGCCCGGTCGAGAGGCCGAGCAGCCCGGCGGCG
ATGTCCTGGTCGAGGGCGAC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLTLGMAASSTMAGPAPDDALVATLRAAGCVFAEDEAALLAREAGDEAALAAMVARRVAGEPLEQVLGFAEFLGLRVAVE
PGVFVPRLRTTLLARRAIRALEPGDLAVDLCCGTGAIGVALLAAVPGAEVHAADIDPAAVRCARRNLPPDRVHEGDLYDA
LPGELRGRVAVVVANAPYVPTGAIATMPPEARDHEHRVALDGGADGLEVARRVIAGARPWLAPGGRVLVETSAAQRDLAA
AHVVAAGLDVTVASAADVDGNVVIGRLHPERLSPERLSPEQLSPGQLSPGQLSPGRPRC

Sequences:

>Translated_299_residues
MLTLGMAASSTMAGPAPDDALVATLRAAGCVFAEDEAALLAREAGDEAALAAMVARRVAGEPLEQVLGFAEFLGLRVAVE
PGVFVPRLRTTLLARRAIRALEPGDLAVDLCCGTGAIGVALLAAVPGAEVHAADIDPAAVRCARRNLPPDRVHEGDLYDA
LPGELRGRVAVVVANAPYVPTGAIATMPPEARDHEHRVALDGGADGLEVARRVIAGARPWLAPGGRVLVETSAAQRDLAA
AHVVAAGLDVTVASAADVDGNVVIGRLHPERLSPERLSPEQLSPGQLSPGQLSPGRPRC
>Mature_299_residues
MLTLGMAASSTMAGPAPDDALVATLRAAGCVFAEDEAALLAREAGDEAALAAMVARRVAGEPLEQVLGFAEFLGLRVAVE
PGVFVPRLRTTLLARRAIRALEPGDLAVDLCCGTGAIGVALLAAVPGAEVHAADIDPAAVRCARRNLPPDRVHEGDLYDA
LPGELRGRVAVVVANAPYVPTGAIATMPPEARDHEHRVALDGGADGLEVARRVIAGARPWLAPGGRVLVETSAAQRDLAA
AHVVAAGLDVTVASAADVDGNVVIGRLHPERLSPERLSPEQLSPGQLSPGQLSPGRPRC

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=211, Percent_Identity=28.9099526066351, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI87082085, Length=209, Percent_Identity=29.6650717703349, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787463, Length=226, Percent_Identity=30.9734513274336, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24582226, Length=210, Percent_Identity=31.4285714285714, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30710; Mature: 30710

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLGMAASSTMAGPAPDDALVATLRAAGCVFAEDEAALLAREAGDEAALAAMVARRVAG
CEEECCCCCCCCCCCCCHHHHHHHHHHHCEEEECCHHHHHHHHCCCHHHHHHHHHHHHCC
EPLEQVLGFAEFLGLRVAVEPGVFVPRLRTTLLARRAIRALEPGDLAVDLCCGTGAIGVA
CHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
LLAAVPGAEVHAADIDPAAVRCARRNLPPDRVHEGDLYDALPGELRGRVAVVVANAPYVP
HHHHCCCCCEEECCCCHHHHHHHHCCCCHHHCCCCCCHHCCCHHHCCCEEEEEECCCCCC
TGAIATMPPEARDHEHRVALDGGADGLEVARRVIAGARPWLAPGGRVLVETSAAQRDLAA
CCCEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHH
AHVVAAGLDVTVASAADVDGNVVIGRLHPERLSPERLSPEQLSPGQLSPGQLSPGRPRC
HHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTLGMAASSTMAGPAPDDALVATLRAAGCVFAEDEAALLAREAGDEAALAAMVARRVAG
CEEECCCCCCCCCCCCCHHHHHHHHHHHCEEEECCHHHHHHHHCCCHHHHHHHHHHHHCC
EPLEQVLGFAEFLGLRVAVEPGVFVPRLRTTLLARRAIRALEPGDLAVDLCCGTGAIGVA
CHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
LLAAVPGAEVHAADIDPAAVRCARRNLPPDRVHEGDLYDALPGELRGRVAVVVANAPYVP
HHHHCCCCCEEECCCCHHHHHHHHCCCCHHHCCCCCCHHCCCHHHCCCEEEEEECCCCCC
TGAIATMPPEARDHEHRVALDGGADGLEVARRVIAGARPWLAPGGRVLVETSAAQRDLAA
CCCEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHH
AHVVAAGLDVTVASAADVDGNVVIGRLHPERLSPERLSPEQLSPGQLSPGQLSPGRPRC
HHHHHHCCCEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]