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The map label for this gene is ulaF [H]

Identifier: 119714646

GI number: 119714646

Start: 410564

End: 411280

Strand: Direct

Name: ulaF [H]

Synonym: Noca_0381

Alternate gene names: 119714646

Gene position: 410564-411280 (Clockwise)

Preceding gene: 119714645

Following gene: 119714647

Centisome position: 8.23

GC content: 72.25

Gene sequence:

>717_bases
ATGAACGCCGTCGTCAGCGCCGAGTCCCGCGAGGCGGTACGCCGGCTGCAGGCGGAGGTCGCCGCGCTGCACGCCGAGCT
GACCCGCAACCGGCTGGTCGTGTGGACCGCCGGCAACGTGTCCGCGCGGGTGCCGGGCACCGACCTGTTCGTGATCAAGC
CGTCGGGGGTCTCCTACGACGAGCTGGACGCCGACACGATGGTCGTCTGCGACCTCGACGGCACCCTGGTCGAGGGGGCG
CGACAGCCGAGCTCCGACACCGCCGCCCACGCCTACGTCTACCGCCACCTGCCCGAGGTCGGCGGGGTCGTGCACACCCA
CTCGACGTACGCGACCGCGTGGGCCGCCCGCGGCGAGCCGATCCCGTGCGTGCTCACGATGGCGGCCGACGAGTTCGGCG
GCGAGATCCCGATCGGGCCGTTCGCGCTGATCGGGGACGACTCGATCGGCCGCGGGATCGTCGAGACGCTGCGCGAGAGC
CGCTCGCGCGCCGTACTCATGCAGAACCACGGCCCGTTCACCGTCGGGCCCGATGCCCGGTCCGCCGTCAAGGCGGCCGT
GATGGTCGAGGACGTCGCTCGCACGGTGCACGTCGCCCGCCAGCTCGGTGCGCCGCTCCCGATCGCCCAGGAGCACGTCG
ACGCCCTCTACGACCGCTACCAGAACGTGTACGGCCAGACCCAGCAGACCCAGCAACAGACCGGAGGAGTCGCGTGA

Upstream 100 bases:

>100_bases
CCGAGTACCGCGCCCTCCACGATCACTTCGGTCGCGGCGGCACCGAGGTGATGCGCCGACTCCGCTCGATCCGCCGCGCC
ACCCACACCGGAGCCCAGGC

Downstream 100 bases:

>100_bases
CCCACCACCCCGACGAGCTCGAGATCTGGTTCCTCACCGGGAGCCAGCACCTGTACGGGCCCGAGGTCATCGACCAGGTC
GCCGAGCACGCCGCCCGGAT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNAVVSAESREAVRRLQAEVAALHAELTRNRLVVWTAGNVSARVPGTDLFVIKPSGVSYDELDADTMVVCDLDGTLVEGA
RQPSSDTAAHAYVYRHLPEVGGVVHTHSTYATAWAARGEPIPCVLTMAADEFGGEIPIGPFALIGDDSIGRGIVETLRES
RSRAVLMQNHGPFTVGPDARSAVKAAVMVEDVARTVHVARQLGAPLPIAQEHVDALYDRYQNVYGQTQQTQQQTGGVA

Sequences:

>Translated_238_residues
MNAVVSAESREAVRRLQAEVAALHAELTRNRLVVWTAGNVSARVPGTDLFVIKPSGVSYDELDADTMVVCDLDGTLVEGA
RQPSSDTAAHAYVYRHLPEVGGVVHTHSTYATAWAARGEPIPCVLTMAADEFGGEIPIGPFALIGDDSIGRGIVETLRES
RSRAVLMQNHGPFTVGPDARSAVKAAVMVEDVARTVHVARQLGAPLPIAQEHVDALYDRYQNVYGQTQQTQQQTGGVA
>Mature_238_residues
MNAVVSAESREAVRRLQAEVAALHAELTRNRLVVWTAGNVSARVPGTDLFVIKPSGVSYDELDADTMVVCDLDGTLVEGA
RQPSSDTAAHAYVYRHLPEVGGVVHTHSTYATAWAARGEPIPCVLTMAADEFGGEIPIGPFALIGDDSIGRGIVETLRES
RSRAVLMQNHGPFTVGPDARSAVKAAVMVEDVARTVHVARQLGAPLPIAQEHVDALYDRYQNVYGQTQQTQQQTGGVA

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=217, Percent_Identity=41.4746543778802, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1790008, Length=235, Percent_Identity=40.4255319148936, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1786247, Length=229, Percent_Identity=39.7379912663755, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789164, Length=201, Percent_Identity=29.8507462686567, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789094, Length=191, Percent_Identity=25.130890052356, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25453; Mature: 25453

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVVSAESREAVRRLQAEVAALHAELTRNRLVVWTAGNVSARVPGTDLFVIKPSGVSYD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCEEEEECCCCCCHH
ELDADTMVVCDLDGTLVEGARQPSSDTAAHAYVYRHLPEVGGVVHTHSTYATAWAARGEP
HCCCCEEEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCC
IPCVLTMAADEFGGEIPIGPFALIGDDSIGRGIVETLRESRSRAVLMQNHGPFTVGPDAR
CCEEEEEEHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCEEECCCHH
SAVKAAVMVEDVARTVHVARQLGAPLPIAQEHVDALYDRYQNVYGQTQQTQQQTGGVA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
>Mature Secondary Structure
MNAVVSAESREAVRRLQAEVAALHAELTRNRLVVWTAGNVSARVPGTDLFVIKPSGVSYD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCEEEEECCCCCCHH
ELDADTMVVCDLDGTLVEGARQPSSDTAAHAYVYRHLPEVGGVVHTHSTYATAWAARGEP
HCCCCEEEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCC
IPCVLTMAADEFGGEIPIGPFALIGDDSIGRGIVETLRESRSRAVLMQNHGPFTVGPDAR
CCEEEEEEHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCEEECCCHH
SAVKAAVMVEDVARTVHVARQLGAPLPIAQEHVDALYDRYQNVYGQTQQTQQQTGGVA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA