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The map label for this gene is nasB [H]

Identifier: 119714618

GI number: 119714618

Start: 376783

End: 379299

Strand: Direct

Name: nasB [H]

Synonym: Noca_0352

Alternate gene names: 119714618

Gene position: 376783-379299 (Clockwise)

Preceding gene: 119714617

Following gene: 119714619

Centisome position: 7.56

GC content: 72.71

Gene sequence:

>2517_bases
ATGAGCCCGAAGCACCGCAGGACCCTCCTGGTCGCCGGCCACGGCATGGTCGGCCACCGGTTCGTGCAGGCCGCGATCGA
GCGCGGCCTCACCGAGACCCACGACATCGTCGTGGTCGGCGAGGAGCGCCGCCCGGCGTACGACCGGGTCGCGCTCACCT
CGTTCTTCGAGGTCGGCGCCGACGAGCTGTCGTTCCTGCCGAGCGGGGAGTACGACGACCCGCGGGTGCGGCTGCTCCTC
GGGACCAGTGTGGACGGGTTCGACCCGGCGACGCGCACCGTGGTGCTCTCCGACGGACAGGAGATCGCCTACGACGAGCT
GGTGCTCGCCACCGGGGCGATGCCGTTCGTGCCGCCCGTGCCCGGCCACGATCTGGAGGGCTGCTTCGTCTACCGCACGA
TCGAGGACCTCGAGGCGATCCGCGCGGCGGCCGCGACCGCCGAGGTCGGCGCGGTCGTCGGCGGCGGGCTGCTCGGGCTC
GAGGCTGCCAACGCACTGGCCCAGCTCGGCCTGGTGACCCACGTCGTGGAGATGGCACCCCGGCTGATGGCGGTGCAGGT
CGACGAAGCCGGCGGCGCCACGCTCGCGCGGCACATCGAGCGGCTCGGGCTCACCGTGCACACCGGCGCGACGACCCAGC
GGATCCTCGGGGCGGACCGGGTCGAGGGCCTGGCGCTGGCCGGCGAGGAGCCGATCCCGGCCGACCTCGTGGTGTTCTCG
GCCGGCATCCGCCCGCGCGATCAGCTGGCCCGCGACGCGGGCCTCGACGTCGCCGAGCGCGGCGGCGTGCTCGTCGACGA
GCGCTGCCGCACCTCCGACCCGCACGTGTGGGCGATCGGCGAGTGTGCCGCGCCGGGCGGCCGGATGTACGGCCTGGTCG
CCCCCGGGTACGCCATGGCCGAGGTCGTGGTCGACGCGCTGCTCGGCGGCTCCGGGGCGTTCACCGGCGCCGACCTGTCC
ACCAAGCTGAAGCTGCTCGGCGTCGACGTCGCGTCCTTCGGGGACGCGTTCGCCGCGACCGAGGGGGCGCTGGAGCTCGT
GTACGCCGACGCCGTCACGGGCGTCTACAAGAAGCTGGTGCTCTCCGCGGACGGCCGGCAGCTGCTCGGCGGGATCCTGG
TGGGCGACGCGTCGGCGTACGGCGTGCTGCGCCCGATGGTCGCCTCCGGCATCGCGCTCCCCGACAACCCCGAGGACCTG
ATCCTCCCGACGGGACGGGGCGTCCTCGAGATCGGGCTGCCCGACGAGGCGCAGGTCTGCTCGTGCAACGACGTCACCAA
GGGCGACATCCTCGCCGCGGCCGCGGCGGGGACGAGTACCCGCGCCGGCGCGACCTGCGGCTCCTGCAAGGCCCAGACCA
AGAGGATCATCGAGGACTACTACGCCAGCCAGGGCAAGGTCGTCGACCGGAGCCTGTGCGAGCACTTCGCGCTCACGCGG
CAGGAGCTGTTCGATGTCGTGGCCGTGCACGGCTACACCCGCTTCGACGACATCGTCGCGGGGCACGGCCGCGGCCGGGG
CTGCGACATCTGCAAGCCCGCGATCGCCTCGATCCTCGCCTCCCAGCTCAACGGGCACGTCCTGGACGGCCCGAACCGGA
CCCTTCAGGACACCAACGACGCCTACCTCGCCAACATCCAGCGCAACGGCACCTACTCCGTGGTCCCCCGCATCCCCGGC
GGCGAGATCACGCCCGAGAAGCTGATCGTGATCGGCGAGGTCGCCCAGGAGTTCGGCCTGTACACCAAGATCACCGGGGG
CCAGCGGATCGACCTCTTCGGCGCCAGGATGGAGCAGCTGCCCGCGATCTGGAGGCGGCTGGTCGACTCCGGCTTCGAGT
CCGGGCACGCCTACGGAAAGTCGCTGCGCACGGTGAAGTCCTGCGTCGGCTCCACCTGGTGCCGCTACGGCGTGCAGGAC
TCCGTGCAGCTGGCCATCGACCTCGAGCTGCGCTACCGCGGCCTGCGCTCGCCGCACAAGCTCAAGGGCGGCGTGAGCGG
CTGCGCGCGTGAGTGCGCCGAGGCGCGGGCCAAGGACTTCGGGGTGATCGCGACCGAGAAGGGCTGGAACCTGTACGTCG
GGGGCAACGGCGGCGCCACCCCCGCGCACGCCCGGCTGCTCGCCGGCGACCTCGACCGCGAGACCCTGGTCCGCTACCTC
GACCGGTTCCTCATGTACTACGTCCGCACCGCCGACCGCCTGCAGCGGACCGCGTCGTGGATCGACTCGATCGACGGCGG
CCTGGACCGGGTGCGGGAGATCGTCGTGGACGACGCGCTCGGCCTCGGCTCGGAACTCGAGGCCGCGATGGCCCGGCACG
TGGCCGGCTACTTCGACGAATGGCGGGCCACCCTCGACGACCCCGCGAAGCTGGCCCGGTTCGTCTCCTTCGTCAACGCC
CCGGGCACCCCGGATCCCCACATCACGTTCCGGTCCGAGCGCGACCAGGCGGTGCCGGAGGAGTCCTCGGGGCCGGTCGA
CCTCGGCGCCACGATCCCGGTGGGAGCGCCGCGGTGA

Upstream 100 bases:

>100_bases
CCACGGGCTGCGGGGGCTGTGCCGCCGCGGTCCGCCAGCTGCTCGCAGCCCGTCTGTCGCCAGGCCCGTCCCTGGGCACC
ACCCTGGAAGGAACCCCCCG

Downstream 100 bases:

>100_bases
GCACGTCGACCGGGCCCGCCGCGGGGGCGACGTACACGACCGTCTGCGGGCTCGACCAGATCGAGGTCGAGGGCGGCGTC
GTGGCGCTCGTCGACGGCGT

Product: assimilatory nitrite reductase (NAD(P)H) large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 838; Mature: 837

Protein sequence:

>838_residues
MSPKHRRTLLVAGHGMVGHRFVQAAIERGLTETHDIVVVGEERRPAYDRVALTSFFEVGADELSFLPSGEYDDPRVRLLL
GTSVDGFDPATRTVVLSDGQEIAYDELVLATGAMPFVPPVPGHDLEGCFVYRTIEDLEAIRAAAATAEVGAVVGGGLLGL
EAANALAQLGLVTHVVEMAPRLMAVQVDEAGGATLARHIERLGLTVHTGATTQRILGADRVEGLALAGEEPIPADLVVFS
AGIRPRDQLARDAGLDVAERGGVLVDERCRTSDPHVWAIGECAAPGGRMYGLVAPGYAMAEVVVDALLGGSGAFTGADLS
TKLKLLGVDVASFGDAFAATEGALELVYADAVTGVYKKLVLSADGRQLLGGILVGDASAYGVLRPMVASGIALPDNPEDL
ILPTGRGVLEIGLPDEAQVCSCNDVTKGDILAAAAAGTSTRAGATCGSCKAQTKRIIEDYYASQGKVVDRSLCEHFALTR
QELFDVVAVHGYTRFDDIVAGHGRGRGCDICKPAIASILASQLNGHVLDGPNRTLQDTNDAYLANIQRNGTYSVVPRIPG
GEITPEKLIVIGEVAQEFGLYTKITGGQRIDLFGARMEQLPAIWRRLVDSGFESGHAYGKSLRTVKSCVGSTWCRYGVQD
SVQLAIDLELRYRGLRSPHKLKGGVSGCARECAEARAKDFGVIATEKGWNLYVGGNGGATPAHARLLAGDLDRETLVRYL
DRFLMYYVRTADRLQRTASWIDSIDGGLDRVREIVVDDALGLGSELEAAMARHVAGYFDEWRATLDDPAKLARFVSFVNA
PGTPDPHITFRSERDQAVPEESSGPVDLGATIPVGAPR

Sequences:

>Translated_838_residues
MSPKHRRTLLVAGHGMVGHRFVQAAIERGLTETHDIVVVGEERRPAYDRVALTSFFEVGADELSFLPSGEYDDPRVRLLL
GTSVDGFDPATRTVVLSDGQEIAYDELVLATGAMPFVPPVPGHDLEGCFVYRTIEDLEAIRAAAATAEVGAVVGGGLLGL
EAANALAQLGLVTHVVEMAPRLMAVQVDEAGGATLARHIERLGLTVHTGATTQRILGADRVEGLALAGEEPIPADLVVFS
AGIRPRDQLARDAGLDVAERGGVLVDERCRTSDPHVWAIGECAAPGGRMYGLVAPGYAMAEVVVDALLGGSGAFTGADLS
TKLKLLGVDVASFGDAFAATEGALELVYADAVTGVYKKLVLSADGRQLLGGILVGDASAYGVLRPMVASGIALPDNPEDL
ILPTGRGVLEIGLPDEAQVCSCNDVTKGDILAAAAAGTSTRAGATCGSCKAQTKRIIEDYYASQGKVVDRSLCEHFALTR
QELFDVVAVHGYTRFDDIVAGHGRGRGCDICKPAIASILASQLNGHVLDGPNRTLQDTNDAYLANIQRNGTYSVVPRIPG
GEITPEKLIVIGEVAQEFGLYTKITGGQRIDLFGARMEQLPAIWRRLVDSGFESGHAYGKSLRTVKSCVGSTWCRYGVQD
SVQLAIDLELRYRGLRSPHKLKGGVSGCARECAEARAKDFGVIATEKGWNLYVGGNGGATPAHARLLAGDLDRETLVRYL
DRFLMYYVRTADRLQRTASWIDSIDGGLDRVREIVVDDALGLGSELEAAMARHVAGYFDEWRATLDDPAKLARFVSFVNA
PGTPDPHITFRSERDQAVPEESSGPVDLGATIPVGAPR
>Mature_837_residues
SPKHRRTLLVAGHGMVGHRFVQAAIERGLTETHDIVVVGEERRPAYDRVALTSFFEVGADELSFLPSGEYDDPRVRLLLG
TSVDGFDPATRTVVLSDGQEIAYDELVLATGAMPFVPPVPGHDLEGCFVYRTIEDLEAIRAAAATAEVGAVVGGGLLGLE
AANALAQLGLVTHVVEMAPRLMAVQVDEAGGATLARHIERLGLTVHTGATTQRILGADRVEGLALAGEEPIPADLVVFSA
GIRPRDQLARDAGLDVAERGGVLVDERCRTSDPHVWAIGECAAPGGRMYGLVAPGYAMAEVVVDALLGGSGAFTGADLST
KLKLLGVDVASFGDAFAATEGALELVYADAVTGVYKKLVLSADGRQLLGGILVGDASAYGVLRPMVASGIALPDNPEDLI
LPTGRGVLEIGLPDEAQVCSCNDVTKGDILAAAAAGTSTRAGATCGSCKAQTKRIIEDYYASQGKVVDRSLCEHFALTRQ
ELFDVVAVHGYTRFDDIVAGHGRGRGCDICKPAIASILASQLNGHVLDGPNRTLQDTNDAYLANIQRNGTYSVVPRIPGG
EITPEKLIVIGEVAQEFGLYTKITGGQRIDLFGARMEQLPAIWRRLVDSGFESGHAYGKSLRTVKSCVGSTWCRYGVQDS
VQLAIDLELRYRGLRSPHKLKGGVSGCARECAEARAKDFGVIATEKGWNLYVGGNGGATPAHARLLAGDLDRETLVRYLD
RFLMYYVRTADRLQRTASWIDSIDGGLDRVREIVVDDALGLGSELEAAMARHVAGYFDEWRATLDDPAKLARFVSFVNAP
GTPDPHITFRSERDQAVPEESSGPVDLGATIPVGAPR

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=275, Percent_Identity=25.4545454545455, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI65787454, Length=275, Percent_Identity=25.4545454545455, Blast_Score=86, Evalue=2e-16,
Organism=Homo sapiens, GI226437568, Length=275, Percent_Identity=25.4545454545455, Blast_Score=86, Evalue=2e-16,
Organism=Escherichia coli, GI1789765, Length=827, Percent_Identity=51.3905683192261, Blast_Score=842, Evalue=0.0,
Organism=Escherichia coli, GI1788892, Length=282, Percent_Identity=28.3687943262411, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789065, Length=319, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=284, Percent_Identity=27.112676056338, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281359715, Length=299, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281359713, Length=299, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639250, Length=299, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI18543267, Length=299, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639252, Length=299, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639257, Length=299, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585130, Length=281, Percent_Identity=24.5551601423488, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581020, Length=225, Percent_Identity=30.6666666666667, Blast_Score=74, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28573993, Length=225, Percent_Identity=30.6666666666667, Blast_Score=74, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 89000; Mature: 88869

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKHRRTLLVAGHGMVGHRFVQAAIERGLTETHDIVVVGEERRPAYDRVALTSFFEVGA
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCC
DELSFLPSGEYDDPRVRLLLGTSVDGFDPATRTVVLSDGQEIAYDELVLATGAMPFVPPV
HHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCC
PGHDLEGCFVYRTIEDLEAIRAAAATAEVGAVVGGGLLGLEAANALAQLGLVTHVVEMAP
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
RLMAVQVDEAGGATLARHIERLGLTVHTGATTQRILGADRVEGLALAGEEPIPADLVVFS
CEEEEEECCCCCHHHHHHHHHHCCEEECCCCHHHHHCHHHHCCEEECCCCCCCCEEEEEE
AGIRPRDQLARDAGLDVAERGGVLVDERCRTSDPHVWAIGECAAPGGRMYGLVAPGYAMA
CCCCCHHHHHHHCCCCHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCEEEEECCCHHHH
EVVVDALLGGSGAFTGADLSTKLKLLGVDVASFGDAFAATEGALELVYADAVTGVYKKLV
HHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCHHHHCCCHHEEHHHHHHHHHHHHHH
LSADGRQLLGGILVGDASAYGVLRPMVASGIALPDNPEDLILPTGRGVLEIGLPDEAQVC
HCCCCHHHHCEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCCCCEE
SCNDVTKGDILAAAAAGTSTRAGATCGSCKAQTKRIIEDYYASQGKVVDRSLCEHFALTR
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QELFDVVAVHGYTRFDDIVAGHGRGRGCDICKPAIASILASQLNGHVLDGPNRTLQDTND
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCCC
AYLANIQRNGTYSVVPRIPGGEITPEKLIVIGEVAQEFGLYTKITGGQRIDLFGARMEQL
EEEEEEECCCCEEEEECCCCCCCCHHHEEEEHHHHHHCCCEEEECCCCEEEHHHHHHHHH
PAIWRRLVDSGFESGHAYGKSLRTVKSCVGSTWCRYGVQDSVQLAIDLELRYRGLRSPHK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHCCCCCCHH
LKGGVSGCARECAEARAKDFGVIATEKGWNLYVGGNGGATPAHARLLAGDLDRETLVRYL
HCCCHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHH
DRFLMYYVRTADRLQRTASWIDSIDGGLDRVREIVVDDALGLGSELEAAMARHVAGYFDE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
WRATLDDPAKLARFVSFVNAPGTPDPHITFRSERDQAVPEESSGPVDLGATIPVGAPR
HHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEECCCCCCCCCCC
>Mature Secondary Structure 
SPKHRRTLLVAGHGMVGHRFVQAAIERGLTETHDIVVVGEERRPAYDRVALTSFFEVGA
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCC
DELSFLPSGEYDDPRVRLLLGTSVDGFDPATRTVVLSDGQEIAYDELVLATGAMPFVPPV
HHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCC
PGHDLEGCFVYRTIEDLEAIRAAAATAEVGAVVGGGLLGLEAANALAQLGLVTHVVEMAP
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
RLMAVQVDEAGGATLARHIERLGLTVHTGATTQRILGADRVEGLALAGEEPIPADLVVFS
CEEEEEECCCCCHHHHHHHHHHCCEEECCCCHHHHHCHHHHCCEEECCCCCCCCEEEEEE
AGIRPRDQLARDAGLDVAERGGVLVDERCRTSDPHVWAIGECAAPGGRMYGLVAPGYAMA
CCCCCHHHHHHHCCCCHHHCCCEEEECCCCCCCCCEEEEECCCCCCCCEEEEECCCHHHH
EVVVDALLGGSGAFTGADLSTKLKLLGVDVASFGDAFAATEGALELVYADAVTGVYKKLV
HHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCHHHHCCCHHEEHHHHHHHHHHHHHH
LSADGRQLLGGILVGDASAYGVLRPMVASGIALPDNPEDLILPTGRGVLEIGLPDEAQVC
HCCCCHHHHCEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCCCCEE
SCNDVTKGDILAAAAAGTSTRAGATCGSCKAQTKRIIEDYYASQGKVVDRSLCEHFALTR
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QELFDVVAVHGYTRFDDIVAGHGRGRGCDICKPAIASILASQLNGHVLDGPNRTLQDTND
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCCC
AYLANIQRNGTYSVVPRIPGGEITPEKLIVIGEVAQEFGLYTKITGGQRIDLFGARMEQL
EEEEEEECCCCEEEEECCCCCCCCHHHEEEEHHHHHHCCCEEEECCCCEEEHHHHHHHHH
PAIWRRLVDSGFESGHAYGKSLRTVKSCVGSTWCRYGVQDSVQLAIDLELRYRGLRSPHK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHCCCCCCHH
LKGGVSGCARECAEARAKDFGVIATEKGWNLYVGGNGGATPAHARLLAGDLDRETLVRYL
HCCCHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHH
DRFLMYYVRTADRLQRTASWIDSIDGGLDRVREIVVDDALGLGSELEAAMARHVAGYFDE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
WRATLDDPAKLARFVSFVNAPGTPDPHITFRSERDQAVPEESSGPVDLGATIPVGAPR
HHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]