Click here to switch to the map view.

The map label for this gene is nasD [H]

Identifier: 119714617

GI number: 119714617

Start: 375404

End: 376786

Strand: Direct

Name: nasD [H]

Synonym: Noca_0351

Alternate gene names: 119714617

Gene position: 375404-376786 (Clockwise)

Preceding gene: 119714616

Following gene: 119714618

Centisome position: 7.53

GC content: 76.43

Gene sequence:

>1383_bases
ATGAGGGTCGTCGTCGTCGGAGCCGGGATGGCCGCCGCCCGCCTCGCCGACGGTCTCCACGACCACGACGTGACCCTGCT
CGGGGACGAGCCGCACCCGCCGTACAACCGGATCCTGCTCTCCGCCGTCCTGGAGGGCACCCATGATCCGACCGCGATCG
CGATGCCGCTGCGCGGCGATGTCGACCTGCGGCTCGGGACCCGCGTCGTGGAGATCCACCGCGAGGACCGCGACGTCGAG
CTCGCCGACCGGTCCCGGGTGCCCTACGACCGGCTGGTGCTCGCCACCGGCAGCATCCCGACGCTCCCGCCGATCCGCGG
GCTGGTGCGGATGGACGGCCGGCTCGACGAGCGCGTGCACGCGTTCCGCAGCCTGGAGGACTGCCGGCGGCTCGACGTGG
CGGTCCCGGCGGCGCACCGGGCGGTCGTCGTCGGCGGCGGGCTGCTGGGCCTGCAGGTCGCGCGGGCGCTCGCGGTCCGC
GGCGTCGCCACCGAGGTCGTCGAGGGCGGTGACCACCTGCTGCGCAGCCAGGTCGGCCCGCGGGCCGGGGCGATCCTGGC
TCGCGACCTCGGCCGGCTGGGCACCCGCGTCTACACCGGCGCCAGGGCCGTCCGGCTGACCGACGAGGGTCTGCGGCTCG
ACAACGGATACACGCTGGCGACCGACCTCGTGGTGCTCACCGCCGGCGGCCGCCCCTCGACCGCGCTGGCCCGGCGGGCC
GGGCTGGAGGTCCGCCGCGGCATCGTGGTCGACGAGCGGCTGCGCACCAGCGACCACCGCATCCACGCGATCGGCGACTG
CGCCCAGCACGGGACACAGGTGACCGGATTCGTGCCGCCGGCGTGGGAGCAGGCCGGGCTGCTGGCACGGCAGCTGCGCG
GCGAGGACGTGACGTACGCCGGCAGCCGCAGCGTCGCCCGGCTGCGGGCGACCGGGCTCGAGGTCGCCGTGCTCGGCGAG
CCCGAGCGCGCCGAGGGCCAGGTGGTCGAGGTCGCCAACCCGGTCACCGGGACCCACCGCAAGCTCGTGGTCCGCGACGG
CGTGATCGTCGCCGCGACCCTGGTCGGCGACCTCTCCCGGATCGGCCTGATCACCCAGCACTACGACCGCGGCACGGTCC
TCGGCGCCAGCGAGCCCGGCGACCTGCTGATCGGCGATCGTCCGGCCGCCCAGGTCGACCTCCCCGACGACGCGGAGGTC
TGCGCGTGCGCGGGCGTGAGCGCCGGGGCGATCCGCGCCTGCGCCTCGCTCGCGGACGCGCGCGAGACCACCCGCGCCAC
CACGGGCTGCGGGGGCTGTGCCGCCGCGGTCCGCCAGCTGCTCGCAGCCCGTCTGTCGCCAGGCCCGTCCCTGGGCACCA
CCCTGGAAGGAACCCCCCGATGA

Upstream 100 bases:

>100_bases
GCTCACCGCTGGCCGGGGGTGGCGGGGGTGGGCGGTGTGTCCGCAACCGTTGGGGGCGCTCATTCGGTTGCCTGCTCACC
GCTGGGCGGGGGGCGGCCGG

Downstream 100 bases:

>100_bases
GCCCGAAGCACCGCAGGACCCTCCTGGTCGCCGGCCACGGCATGGTCGGCCACCGGTTCGTGCAGGCCGCGATCGAGCGC
GGCCTCACCGAGACCCACGA

Product: assimilatory nitrate reductase (NADH) subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MRVVVVGAGMAAARLADGLHDHDVTLLGDEPHPPYNRILLSAVLEGTHDPTAIAMPLRGDVDLRLGTRVVEIHREDRDVE
LADRSRVPYDRLVLATGSIPTLPPIRGLVRMDGRLDERVHAFRSLEDCRRLDVAVPAAHRAVVVGGGLLGLQVARALAVR
GVATEVVEGGDHLLRSQVGPRAGAILARDLGRLGTRVYTGARAVRLTDEGLRLDNGYTLATDLVVLTAGGRPSTALARRA
GLEVRRGIVVDERLRTSDHRIHAIGDCAQHGTQVTGFVPPAWEQAGLLARQLRGEDVTYAGSRSVARLRATGLEVAVLGE
PERAEGQVVEVANPVTGTHRKLVVRDGVIVAATLVGDLSRIGLITQHYDRGTVLGASEPGDLLIGDRPAAQVDLPDDAEV
CACAGVSAGAIRACASLADARETTRATTGCGGCAAAVRQLLAARLSPGPSLGTTLEGTPR

Sequences:

>Translated_460_residues
MRVVVVGAGMAAARLADGLHDHDVTLLGDEPHPPYNRILLSAVLEGTHDPTAIAMPLRGDVDLRLGTRVVEIHREDRDVE
LADRSRVPYDRLVLATGSIPTLPPIRGLVRMDGRLDERVHAFRSLEDCRRLDVAVPAAHRAVVVGGGLLGLQVARALAVR
GVATEVVEGGDHLLRSQVGPRAGAILARDLGRLGTRVYTGARAVRLTDEGLRLDNGYTLATDLVVLTAGGRPSTALARRA
GLEVRRGIVVDERLRTSDHRIHAIGDCAQHGTQVTGFVPPAWEQAGLLARQLRGEDVTYAGSRSVARLRATGLEVAVLGE
PERAEGQVVEVANPVTGTHRKLVVRDGVIVAATLVGDLSRIGLITQHYDRGTVLGASEPGDLLIGDRPAAQVDLPDDAEV
CACAGVSAGAIRACASLADARETTRATTGCGGCAAAVRQLLAARLSPGPSLGTTLEGTPR
>Mature_460_residues
MRVVVVGAGMAAARLADGLHDHDVTLLGDEPHPPYNRILLSAVLEGTHDPTAIAMPLRGDVDLRLGTRVVEIHREDRDVE
LADRSRVPYDRLVLATGSIPTLPPIRGLVRMDGRLDERVHAFRSLEDCRRLDVAVPAAHRAVVVGGGLLGLQVARALAVR
GVATEVVEGGDHLLRSQVGPRAGAILARDLGRLGTRVYTGARAVRLTDEGLRLDNGYTLATDLVVLTAGGRPSTALARRA
GLEVRRGIVVDERLRTSDHRIHAIGDCAQHGTQVTGFVPPAWEQAGLLARQLRGEDVTYAGSRSVARLRATGLEVAVLGE
PERAEGQVVEVANPVTGTHRKLVVRDGVIVAATLVGDLSRIGLITQHYDRGTVLGASEPGDLLIGDRPAAQVDLPDDAEV
CACAGVSAGAIRACASLADARETTRATTGCGGCAAAVRQLLAARLSPGPSLGTTLEGTPR

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI22202629, Length=345, Percent_Identity=25.7971014492754, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI4757732, Length=345, Percent_Identity=25.7971014492754, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI65787454, Length=281, Percent_Identity=26.6903914590747, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI226437568, Length=281, Percent_Identity=26.6903914590747, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI21389617, Length=281, Percent_Identity=26.6903914590747, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789765, Length=484, Percent_Identity=30.7851239669421, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789065, Length=337, Percent_Identity=29.3768545994065, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1788892, Length=278, Percent_Identity=29.136690647482, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787352, Length=303, Percent_Identity=27.3927392739274, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=290, Percent_Identity=26.8965517241379, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 48403; Mature: 48403

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVVVGAGMAAARLADGLHDHDVTLLGDEPHPPYNRILLSAVLEGTHDPTAIAMPLRGD
CEEEEEECCHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
VDLRLGTRVVEIHREDRDVELADRSRVPYDRLVLATGSIPTLPPIRGLVRMDGRLDERVH
EEEEECCEEEEEECCCCCCEEHHCCCCCHHHEEEEECCCCCCCHHHHHHHCCCCHHHHHH
AFRSLEDCRRLDVAVPAAHRAVVVGGGLLGLQVARALAVRGVATEVVEGGDHLLRSQVGP
HHHHHHHHHHCCEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
RAGAILARDLGRLGTRVYTGARAVRLTDEGLRLDNGYTLATDLVVLTAGGRPSTALARRA
CHHHHHHHHHHHHHHHEECCCEEEEEECCCEEECCCEEEEEEEEEEECCCCCHHHHHHHH
GLEVRRGIVVDERLRTSDHRIHAIGDCAQHGTQVTGFVPPAWEQAGLLARQLRGEDVTYA
CCHHHCCEEECHHHCCCCCEEEEEHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEC
GSRSVARLRATGLEVAVLGEPERAEGQVVEVANPVTGTHRKLVVRDGVIVAATLVGDLSR
CCCCHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEECCEEEEHHHHHHHHH
IGLITQHYDRGTVLGASEPGDLLIGDRPAAQVDLPDDAEVCACAGVSAGAIRACASLADA
HHHHEEECCCCEEECCCCCCCEEECCCCCCEECCCCCCCHHEECCCCHHHHHHHHHHHHH
RETTRATTGCGGCAAAVRQLLAARLSPGPSLGTTLEGTPR
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVVVVGAGMAAARLADGLHDHDVTLLGDEPHPPYNRILLSAVLEGTHDPTAIAMPLRGD
CEEEEEECCHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCC
VDLRLGTRVVEIHREDRDVELADRSRVPYDRLVLATGSIPTLPPIRGLVRMDGRLDERVH
EEEEECCEEEEEECCCCCCEEHHCCCCCHHHEEEEECCCCCCCHHHHHHHCCCCHHHHHH
AFRSLEDCRRLDVAVPAAHRAVVVGGGLLGLQVARALAVRGVATEVVEGGDHLLRSQVGP
HHHHHHHHHHCCEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
RAGAILARDLGRLGTRVYTGARAVRLTDEGLRLDNGYTLATDLVVLTAGGRPSTALARRA
CHHHHHHHHHHHHHHHEECCCEEEEEECCCEEECCCEEEEEEEEEEECCCCCHHHHHHHH
GLEVRRGIVVDERLRTSDHRIHAIGDCAQHGTQVTGFVPPAWEQAGLLARQLRGEDVTYA
CCHHHCCEEECHHHCCCCCEEEEEHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEC
GSRSVARLRATGLEVAVLGEPERAEGQVVEVANPVTGTHRKLVVRDGVIVAATLVGDLSR
CCCCHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEECCEEEEHHHHHHHHH
IGLITQHYDRGTVLGASEPGDLLIGDRPAAQVDLPDDAEVCACAGVSAGAIRACASLADA
HHHHEEECCCCEEECCCCCCCEEECCCCCCEECCCCCCCHHEECCCCHHHHHHHHHHHHH
RETTRATTGCGGCAAAVRQLLAARLSPGPSLGTTLEGTPR
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]