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The map label for this gene is bmr3 [H]

Identifier: 119714537

GI number: 119714537

Start: 292108

End: 293601

Strand: Direct

Name: bmr3 [H]

Synonym: Noca_0270

Alternate gene names: 119714537

Gene position: 292108-293601 (Clockwise)

Preceding gene: 119714533

Following gene: 119714538

Centisome position: 5.86

GC content: 72.36

Gene sequence:

>1494_bases
GTGCCGGCGCCGGGAATGTCGGCGACCGGGTGGGCGTTGCTTTCCGACGTGGCCTTGTCGGACGTCTCCCGCGCGAGCGT
GGGCCTGCGTTCGGAGCGCGGGCCGGTGCTGCTGGCGGTCATGCTCAGCAACGGGCTGGTCGCGATCGACTCGACCATCC
TCGCGACCGCCGTGCCCGCGGTGGTCACCGACCTCGGTGGGTTCACCCAGTTCCCGTGGTTGTTCTCGGTCTACCTGCTC
GCGCAGGCAGTGTCCGTGCCGATCTACGCCAAGCTGGCCGATCTCTACGGGCGCAAGCCGATGCTGCTGCTCGGCATCGG
GCTGTTCGTCCTCGGCTCGCTGCTGTGCGGCCTGGCCTGGGGGATGGGCGCGCTGATCGCGTTCCGGGCCCTGCAGGGCC
TGGGGGCGGGTGCCGTGCAGCCGATCGGCCTGACCGTGGTCGGCGACATCTACTCGATCCAGGAGCGCGCGAAGGTCCAG
GGCTACCTGGCGAGCGTCTGGGCGATCTCCGCGATCGTCGGGCCGACCCTCGGCGGCGCCTTCTCCGACTACCTGTCGTG
GCGCTGGATCTTCTTCGTGAACCTGCCGCTGGGCGTCGCGGCGGCCTGGATGTTCTGGCGCCGCTTCGACGAGCGCGTCG
AGCGCCGCCGGCACCGGATCGACCACGCCGGCGCCGTGCTGCTGGCGGGTGGTGGGTTGTTGCTGATGCTCGCGCTGCTC
GAGGGCGGAGTGCGCTGGCCGTGGTCGTCGGTCACCAGCGTGGCGCTCTTCTCGGGGGCTGCCGTCCTGCTGGTCGGCTT
CGTCCTGGTCGAGCGGCGGGCCGCGGAGCCGATCCTGCCGCTGTGGGTGTTCCGGCACCGGGTGCTCGGCTCGGCGATGT
CGGCCTCGCTGATTGTCGGCGTGCTCGTGATGGGCCTCGCGTCGTACGTCCCGCTCTACGCCCAGGCCGTGCTCGGCCAC
GGCGCCGTCGTCGCCGGGTTCGCGCTCGCCGGGATGACACTCGGGTGGCCGCTCGCCTCCTCGAGCGCCGGCCGGCTCTT
CCTCACGATCGGTCCCCGCTCCACGATGATGCTCGGCGGTGCGACCACCGCCGCCGGCGCCGCGCTGCTTCTGACGGTGG
GGGCGGACAGCTCGTTCCTCCACCTCGCGGTCCCCTGCTTCGTCATGGGCCTGGGTTTCGGCCTGGTGGCCAGCCCGGCC
GTGATCGCCGCGCAGTCGGCGGTGACCTGGCAGGACCGCGGCGTCGCGACCGGCTCGACCATGTTCGCCCGTTCCATGGG
CAGCGCGATCGGGGTCGCGGTCTTCGGTGCGGTCGCCAACGGCGTGGTCGCCTCCCGGCTCGGCAGCCGGGCCGGCAGCC
TCGAGCTCGTCACGCCCCGGGTGCTGGAGCCGGCGATCGGCGCCGTGTTCCTGACCTCCGCGGTCGTCGCGCTCTCACTG
GTCGCGATCTCGGCGGTGATGACGCGGCGGATCGCCGAGCCGGTTACCGACTAG

Upstream 100 bases:

>100_bases
CGGTTGGAACGATCAAAGCACACCCCTCGGTCTGGTCCGTCAACCCGAGGAGCGCCGTACCCACTGTGCCGCGTGCCCAA
CCCGGCGACCAGCCCCGCGA

Downstream 100 bases:

>100_bases
TAGCAACCGTCGTAGGATGCCCGCCATGAAGCGCAGCATCTACGACGACGACCACGAGGCGTTCCGCGACTCGGTGCGCG
AGTTCCTCGAGCGGTCGGTC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MPAPGMSATGWALLSDVALSDVSRASVGLRSERGPVLLAVMLSNGLVAIDSTILATAVPAVVTDLGGFTQFPWLFSVYLL
AQAVSVPIYAKLADLYGRKPMLLLGIGLFVLGSLLCGLAWGMGALIAFRALQGLGAGAVQPIGLTVVGDIYSIQERAKVQ
GYLASVWAISAIVGPTLGGAFSDYLSWRWIFFVNLPLGVAAAWMFWRRFDERVERRRHRIDHAGAVLLAGGGLLLMLALL
EGGVRWPWSSVTSVALFSGAAVLLVGFVLVERRAAEPILPLWVFRHRVLGSAMSASLIVGVLVMGLASYVPLYAQAVLGH
GAVVAGFALAGMTLGWPLASSSAGRLFLTIGPRSTMMLGGATTAAGAALLLTVGADSSFLHLAVPCFVMGLGFGLVASPA
VIAAQSAVTWQDRGVATGSTMFARSMGSAIGVAVFGAVANGVVASRLGSRAGSLELVTPRVLEPAIGAVFLTSAVVALSL
VAISAVMTRRIAEPVTD

Sequences:

>Translated_497_residues
MPAPGMSATGWALLSDVALSDVSRASVGLRSERGPVLLAVMLSNGLVAIDSTILATAVPAVVTDLGGFTQFPWLFSVYLL
AQAVSVPIYAKLADLYGRKPMLLLGIGLFVLGSLLCGLAWGMGALIAFRALQGLGAGAVQPIGLTVVGDIYSIQERAKVQ
GYLASVWAISAIVGPTLGGAFSDYLSWRWIFFVNLPLGVAAAWMFWRRFDERVERRRHRIDHAGAVLLAGGGLLLMLALL
EGGVRWPWSSVTSVALFSGAAVLLVGFVLVERRAAEPILPLWVFRHRVLGSAMSASLIVGVLVMGLASYVPLYAQAVLGH
GAVVAGFALAGMTLGWPLASSSAGRLFLTIGPRSTMMLGGATTAAGAALLLTVGADSSFLHLAVPCFVMGLGFGLVASPA
VIAAQSAVTWQDRGVATGSTMFARSMGSAIGVAVFGAVANGVVASRLGSRAGSLELVTPRVLEPAIGAVFLTSAVVALSL
VAISAVMTRRIAEPVTD
>Mature_496_residues
PAPGMSATGWALLSDVALSDVSRASVGLRSERGPVLLAVMLSNGLVAIDSTILATAVPAVVTDLGGFTQFPWLFSVYLLA
QAVSVPIYAKLADLYGRKPMLLLGIGLFVLGSLLCGLAWGMGALIAFRALQGLGAGAVQPIGLTVVGDIYSIQERAKVQG
YLASVWAISAIVGPTLGGAFSDYLSWRWIFFVNLPLGVAAAWMFWRRFDERVERRRHRIDHAGAVLLAGGGLLLMLALLE
GGVRWPWSSVTSVALFSGAAVLLVGFVLVERRAAEPILPLWVFRHRVLGSAMSASLIVGVLVMGLASYVPLYAQAVLGHG
AVVAGFALAGMTLGWPLASSSAGRLFLTIGPRSTMMLGGATTAAGAALLLTVGADSSFLHLAVPCFVMGLGFGLVASPAV
IAAQSAVTWQDRGVATGSTMFARSMGSAIGVAVFGAVANGVVASRLGSRAGSLELVTPRVLEPAIGAVFLTSAVVALSLV
AISAVMTRRIAEPVTD

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=27.8325123152709, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=26.491646778043, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789042, Length=404, Percent_Identity=26.980198019802, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788710, Length=411, Percent_Identity=26.2773722627737, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788392, Length=290, Percent_Identity=31.0344827586207, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790146, Length=144, Percent_Identity=32.6388888888889, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=584, Percent_Identity=25.3424657534247, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6325455, Length=469, Percent_Identity=23.6673773987207, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6323735, Length=292, Percent_Identity=28.4246575342466, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6319770, Length=392, Percent_Identity=25.765306122449, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=33.5570469798658, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319783, Length=458, Percent_Identity=24.235807860262, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51384; Mature: 51253

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPGMSATGWALLSDVALSDVSRASVGLRSERGPVLLAVMLSNGLVAIDSTILATAVPA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCEEEEHHHHHHHHHHH
VVTDLGGFTQFPWLFSVYLLAQAVSVPIYAKLADLYGRKPMLLLGIGLFVLGSLLCGLAW
HHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GMGALIAFRALQGLGAGAVQPIGLTVVGDIYSIQERAKVQGYLASVWAISAIVGPTLGGA
HHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FSDYLSWRWIFFVNLPLGVAAAWMFWRRFDERVERRRHRIDHAGAVLLAGGGLLLMLALL
HHHHHCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
EGGVRWPWSSVTSVALFSGAAVLLVGFVLVERRAAEPILPLWVFRHRVLGSAMSASLIVG
HCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
VLVMGLASYVPLYAQAVLGHGAVVAGFALAGMTLGWPLASSSAGRLFLTIGPRSTMMLGG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEECC
ATTAAGAALLLTVGADSSFLHLAVPCFVMGLGFGLVASPAVIAAQSAVTWQDRGVATGST
CCHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEECCCCCCCCHH
MFARSMGSAIGVAVFGAVANGVVASRLGSRAGSLELVTPRVLEPAIGAVFLTSAVVALSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
VAISAVMTRRIAEPVTD
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PAPGMSATGWALLSDVALSDVSRASVGLRSERGPVLLAVMLSNGLVAIDSTILATAVPA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCEEEEHHHHHHHHHHH
VVTDLGGFTQFPWLFSVYLLAQAVSVPIYAKLADLYGRKPMLLLGIGLFVLGSLLCGLAW
HHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GMGALIAFRALQGLGAGAVQPIGLTVVGDIYSIQERAKVQGYLASVWAISAIVGPTLGGA
HHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FSDYLSWRWIFFVNLPLGVAAAWMFWRRFDERVERRRHRIDHAGAVLLAGGGLLLMLALL
HHHHHCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
EGGVRWPWSSVTSVALFSGAAVLLVGFVLVERRAAEPILPLWVFRHRVLGSAMSASLIVG
HCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
VLVMGLASYVPLYAQAVLGHGAVVAGFALAGMTLGWPLASSSAGRLFLTIGPRSTMMLGG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEECC
ATTAAGAALLLTVGADSSFLHLAVPCFVMGLGFGLVASPAVIAAQSAVTWQDRGVATGST
CCHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEECCCCCCCCHH
MFARSMGSAIGVAVFGAVANGVVASRLGSRAGSLELVTPRVLEPAIGAVFLTSAVVALSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
VAISAVMTRRIAEPVTD
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]