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The map label for this gene is mhpC2 [H]

Identifier: 119714477

GI number: 119714477

Start: 225787

End: 226626

Strand: Direct

Name: mhpC2 [H]

Synonym: Noca_0210

Alternate gene names: 119714477

Gene position: 225787-226626 (Clockwise)

Preceding gene: 119714476

Following gene: 119714478

Centisome position: 4.53

GC content: 70.95

Gene sequence:

>840_bases
GTGATCCGCACCCGCGACGTCGAGGTCGGCGACCTGTCCTTCCGGCTCTACGAGACCGGCGAGACCGGGGCCGAGGCCGT
GCTGTTCCTGCACGGCTCGGGCCCCGGCGCCACCGGCCTGTCGAACTGGGAACGGATCATCGAGGACCTCGGCGACCGGT
TCCACTGCCTGGCACCCGACATGATCGGGTTCGGCGACTCCGCGCACCCGGTCGACCCGCCGCGTGGGATGGGTCCCTTC
AACGAGCTGCGCGCCCAGGCGCTGCTGGGCCTGCTCGACGTGCTCGGCCTGGAGAAGGTGCACCTGGTCGGCAACTCGAT
GGGCGGCCAGCTCGCGCTGCTCATGGTGCTCGCCCAGCCCGACCGGGTCGGCAAGATCCTGCTGATGGGCAGCGGCGGCG
CCCCGGACCTGCCCGTCTCCCCGGGCCTGGCCCACCTGCGCACGTTCTACGACGCGCCTTCGGCGGAGAGTCTCGAGCGG
CTGCTCGAGCAGTTCGTCTTCGACCTCGAGCCGATGCGTCCGGTGATCGACCGGGTGGTCAAGGAGCGGATGGCGTACGT
CGAGCGCGCCGACGTGCGCCGCTCGCACGCCGCCAGCTTCGACCCCGACGGGCCGCGGCGTTTCTTCACCCCCGAGGAGC
TGGCCGGCATCGAGCACGACGTCCTCGTCGTGCACGGCCGCGACGACCGGATCATCCCGACCCAGGCGAGCCGGTGGTTC
GCCGACCACCTGCCGAACGCGAACCTCTACGTGATCGGCCGGTGCGGGCACTGGACCCAGATCGAGCACCCGACGACGTT
CGAGACGCTGTTGACCGGTCTCGTCGACGGCGTGATCTAG

Upstream 100 bases:

>100_bases
TCGTCTCCGCGAGCAGCCACGCGTTCCTCTACGCCGACCCGCTGGCGCGGGCCTGGGCGGGCCGGCACCTCGGTGCCGAG
AACCTGGTCGAGGTGCACCT

Downstream 100 bases:

>100_bases
GAGTCGCGGGATGCGGATCGCCAACCACGACGGACGCGCCGTACTCGTGCTGGGCGAGGACAAGGGCGTCGACATCGAGC
GCGCCAGCCACGGCGAGCTC

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase 2; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase 2; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase 2 [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MIRTRDVEVGDLSFRLYETGETGAEAVLFLHGSGPGATGLSNWERIIEDLGDRFHCLAPDMIGFGDSAHPVDPPRGMGPF
NELRAQALLGLLDVLGLEKVHLVGNSMGGQLALLMVLAQPDRVGKILLMGSGGAPDLPVSPGLAHLRTFYDAPSAESLER
LLEQFVFDLEPMRPVIDRVVKERMAYVERADVRRSHAASFDPDGPRRFFTPEELAGIEHDVLVVHGRDDRIIPTQASRWF
ADHLPNANLYVIGRCGHWTQIEHPTTFETLLTGLVDGVI

Sequences:

>Translated_279_residues
MIRTRDVEVGDLSFRLYETGETGAEAVLFLHGSGPGATGLSNWERIIEDLGDRFHCLAPDMIGFGDSAHPVDPPRGMGPF
NELRAQALLGLLDVLGLEKVHLVGNSMGGQLALLMVLAQPDRVGKILLMGSGGAPDLPVSPGLAHLRTFYDAPSAESLER
LLEQFVFDLEPMRPVIDRVVKERMAYVERADVRRSHAASFDPDGPRRFFTPEELAGIEHDVLVVHGRDDRIIPTQASRWF
ADHLPNANLYVIGRCGHWTQIEHPTTFETLLTGLVDGVI
>Mature_279_residues
MIRTRDVEVGDLSFRLYETGETGAEAVLFLHGSGPGATGLSNWERIIEDLGDRFHCLAPDMIGFGDSAHPVDPPRGMGPF
NELRAQALLGLLDVLGLEKVHLVGNSMGGQLALLMVLAQPDRVGKILLMGSGGAPDLPVSPGLAHLRTFYDAPSAESLER
LLEQFVFDLEPMRPVIDRVVKERMAYVERADVRRSHAASFDPDGPRRFFTPEELAGIEHDVLVVHGRDDRIIPTQASRWF
ADHLPNANLYVIGRCGHWTQIEHPTTFETLLTGLVDGVI

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=284, Percent_Identity=32.7464788732394, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30687; Mature: 30687

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTRDVEVGDLSFRLYETGETGAEAVLFLHGSGPGATGLSNWERIIEDLGDRFHCLAPD
CCCCCCCEECCEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCC
MIGFGDSAHPVDPPRGMGPFNELRAQALLGLLDVLGLEKVHLVGNSMGGQLALLMVLAQP
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEHHHHCCC
DRVGKILLMGSGGAPDLPVSPGLAHLRTFYDAPSAESLERLLEQFVFDLEPMRPVIDRVV
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KERMAYVERADVRRSHAASFDPDGPRRFFTPEELAGIEHDVLVVHGRDDRIIPTQASRWF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCHHHHHH
ADHLPNANLYVIGRCGHWTQIEHPTTFETLLTGLVDGVI
HHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRTRDVEVGDLSFRLYETGETGAEAVLFLHGSGPGATGLSNWERIIEDLGDRFHCLAPD
CCCCCCCEECCEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCC
MIGFGDSAHPVDPPRGMGPFNELRAQALLGLLDVLGLEKVHLVGNSMGGQLALLMVLAQP
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEHHHHCCC
DRVGKILLMGSGGAPDLPVSPGLAHLRTFYDAPSAESLERLLEQFVFDLEPMRPVIDRVV
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
KERMAYVERADVRRSHAASFDPDGPRRFFTPEELAGIEHDVLVVHGRDDRIIPTQASRWF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCCCHHHHHH
ADHLPNANLYVIGRCGHWTQIEHPTTFETLLTGLVDGVI
HHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA