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The map label for this gene is bcr [H]

Identifier: 119387176

GI number: 119387176

Start: 1684901

End: 1686109

Strand: Direct

Name: bcr [H]

Synonym: Pden_4471

Alternate gene names: 119387176

Gene position: 1684901-1686109 (Clockwise)

Preceding gene: 119387175

Following gene: 119387177

Centisome position: 97.39

GC content: 69.56

Gene sequence:

>1209_bases
ATGAGCAGCGCGCCCCCCTCCACCCCCCGCCGTCCAAGCATCCTGACGCTGGTTTCGATCTCGGCCGCCGGGGCGCTTTC
GATGAATATCTTCCTGCCCTCGCTGCCGGCCATGGCGCGGGATTTCGGCGTGGAATACGGCTTCATGCAGCTGTCCGTCT
CGGCCTTCCTGGCGGTCAGCGCGGTCCTGCAACTGCTTTGCGGCCCGATCAGCGACCGTTTTGGGCGCCGGCCGGTGGTG
CTGGGCTCGTTTGCGATCTTCCTGCTGGCGACCCTTGGGGCGCTGCTGGCCACGGATGCCGGCTGGTTCATGCTTTTCCG
CCTGATCCAGGCGGTGGTGACGACCGGCTTCGTCATCAGCCGTGCCGTGGTGCGCGACATCGTGCCGGCCGAACAGGCGG
CCAGCATGATCGGCTATGTCACCATGGGCATGTCGCTGGTGCCGATGGTCGCGCCCACCCTGGGCGGCGTTCTGGACGAG
ACGCTGGGCTGGCGGGCCAGCTTCGTCGCCATGGGGCTGGCCGGGCTGGCGGTCATGACGCTGTGCGCCTTCGACCTGAC
CGAGACGGCGCGCGGCGGCGGCGTGCCGCTGCGCCAGCAGGTCGCGACCTATCCGGTCCTGGCCCGTTCGCAGCGGTTCT
GGGGCTATGCGCTGGCCGCGACGCTCAGCGCCGGATCGTTCTACGCCTATCTGGGCGGCGCGCCCTTTGTCGGCCAGGTG
GTGCTGCACCTGTCGCCGGCCGAGGTCGGCTACTGGTTCGCCGGCCCCTCGATCGGCTATGCGCTGGGGAATTACCTTTC
GGCCCGGTTCTCGACCCGGATCGGCATGAACCGGATGATCCTGTCGGGAGCGCTGATCTGCTCGGTCTTCCTAGTGCTGG
CGCTGGTGGTCGACCTGATGGGCGGGCTGACGCCGCTGGTCTTCTTCGGCTCGGTCGGGCTGATGGGGCTGGGCAACGGC
ATGCTCCTGCCCAACGCCAATGCCGGGATGATGAGCGTGCGCCCCGAACTGGCCGGCACCGCCAGCGGGCTGGGCGGCGC
CATGGCGGTGGCGGGCGGCGCGGGCCTGGCCGCGCTGGCCGGCGCGCTGCTGCACGACGATACCGGCGCCTTGCCGCTGC
TGGTCATCATGGCGGGGTCGGCCGTCGCCTCGATCGGCTTCATCCTGTGGGTCATGATCCGCGAAAGGCAGGTCATCAGC
CGCGGCTGA

Upstream 100 bases:

>100_bases
CCTGATCTTCCACAATTCCGGCAGCACTGCGCATTGACGCGAAACAGTACCGCGTAAACAGGCGATTGCCTTCGCCCGCC
GGCCGGGCAAAGGTGCGGGC

Downstream 100 bases:

>100_bases
TCCCTCTTGCGGCAAGGCATTTGCAAAGCGTATCCCTGTCTTTGCAAAGCCAGGAGGTCAGATGGCCACCCAGAAGATCT
ATGCCGGCGTCGCGCTGCGC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSSAPPSTPRRPSILTLVSISAAGALSMNIFLPSLPAMARDFGVEYGFMQLSVSAFLAVSAVLQLLCGPISDRFGRRPVV
LGSFAIFLLATLGALLATDAGWFMLFRLIQAVVTTGFVISRAVVRDIVPAEQAASMIGYVTMGMSLVPMVAPTLGGVLDE
TLGWRASFVAMGLAGLAVMTLCAFDLTETARGGGVPLRQQVATYPVLARSQRFWGYALAATLSAGSFYAYLGGAPFVGQV
VLHLSPAEVGYWFAGPSIGYALGNYLSARFSTRIGMNRMILSGALICSVFLVLALVVDLMGGLTPLVFFGSVGLMGLGNG
MLLPNANAGMMSVRPELAGTASGLGGAMAVAGGAGLAALAGALLHDDTGALPLLVIMAGSAVASIGFILWVMIRERQVIS
RG

Sequences:

>Translated_402_residues
MSSAPPSTPRRPSILTLVSISAAGALSMNIFLPSLPAMARDFGVEYGFMQLSVSAFLAVSAVLQLLCGPISDRFGRRPVV
LGSFAIFLLATLGALLATDAGWFMLFRLIQAVVTTGFVISRAVVRDIVPAEQAASMIGYVTMGMSLVPMVAPTLGGVLDE
TLGWRASFVAMGLAGLAVMTLCAFDLTETARGGGVPLRQQVATYPVLARSQRFWGYALAATLSAGSFYAYLGGAPFVGQV
VLHLSPAEVGYWFAGPSIGYALGNYLSARFSTRIGMNRMILSGALICSVFLVLALVVDLMGGLTPLVFFGSVGLMGLGNG
MLLPNANAGMMSVRPELAGTASGLGGAMAVAGGAGLAALAGALLHDDTGALPLLVIMAGSAVASIGFILWVMIRERQVIS
RG
>Mature_401_residues
SSAPPSTPRRPSILTLVSISAAGALSMNIFLPSLPAMARDFGVEYGFMQLSVSAFLAVSAVLQLLCGPISDRFGRRPVVL
GSFAIFLLATLGALLATDAGWFMLFRLIQAVVTTGFVISRAVVRDIVPAEQAASMIGYVTMGMSLVPMVAPTLGGVLDET
LGWRASFVAMGLAGLAVMTLCAFDLTETARGGGVPLRQQVATYPVLARSQRFWGYALAATLSAGSFYAYLGGAPFVGQVV
LHLSPAEVGYWFAGPSIGYALGNYLSARFSTRIGMNRMILSGALICSVFLVLALVVDLMGGLTPLVFFGSVGLMGLGNGM
LLPNANAGMMSVRPELAGTASGLGGAMAVAGGAGLAALAGALLHDDTGALPLLVIMAGSAVASIGFILWVMIRERQVISR
G

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=372, Percent_Identity=30.9139784946237, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI48994889, Length=348, Percent_Identity=29.0229885057471, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87082312, Length=354, Percent_Identity=29.0960451977401, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790146, Length=163, Percent_Identity=28.8343558282209, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790794, Length=322, Percent_Identity=25.1552795031056, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787065, Length=276, Percent_Identity=25.7246376811594, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787947, Length=250, Percent_Identity=28.8, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=179, Percent_Identity=30.7262569832402, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41541; Mature: 41410

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAPPSTPRRPSILTLVSISAAGALSMNIFLPSLPAMARDFGVEYGFMQLSVSAFLAVS
CCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHH
AVLQLLCGPISDRFGRRPVVLGSFAIFLLATLGALLATDAGWFMLFRLIQAVVTTGFVIS
HHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RAVVRDIVPAEQAASMIGYVTMGMSLVPMVAPTLGGVLDETLGWRASFVAMGLAGLAVMT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCAFDLTETARGGGVPLRQQVATYPVLARSQRFWGYALAATLSAGSFYAYLGGAPFVGQV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHH
VLHLSPAEVGYWFAGPSIGYALGNYLSARFSTRIGMNRMILSGALICSVFLVLALVVDLM
HHEECHHHCCEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
GGLTPLVFFGSVGLMGLGNGMLLPNANAGMMSVRPELAGTASGLGGAMAVAGGAGLAALA
HHHHHHHHHHCCHHHCCCCCEECCCCCCCCEEECHHHHCCCCCCCCHHHHCCCCCHHHHH
GALLHDDTGALPLLVIMAGSAVASIGFILWVMIRERQVISRG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSAPPSTPRRPSILTLVSISAAGALSMNIFLPSLPAMARDFGVEYGFMQLSVSAFLAVS
CCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHH
AVLQLLCGPISDRFGRRPVVLGSFAIFLLATLGALLATDAGWFMLFRLIQAVVTTGFVIS
HHHHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RAVVRDIVPAEQAASMIGYVTMGMSLVPMVAPTLGGVLDETLGWRASFVAMGLAGLAVMT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LCAFDLTETARGGGVPLRQQVATYPVLARSQRFWGYALAATLSAGSFYAYLGGAPFVGQV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHH
VLHLSPAEVGYWFAGPSIGYALGNYLSARFSTRIGMNRMILSGALICSVFLVLALVVDLM
HHEECHHHCCEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
GGLTPLVFFGSVGLMGLGNGMLLPNANAGMMSVRPELAGTASGLGGAMAVAGGAGLAALA
HHHHHHHHHHCCHHHCCCCCEECCCCCCCCEEECHHHHCCCCCCCCHHHHCCCCCHHHHH
GALLHDDTGALPLLVIMAGSAVASIGFILWVMIRERQVISRG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]