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The map label for this gene is glnA [H]

Identifier: 119387167

GI number: 119387167

Start: 1674857

End: 1676263

Strand: Direct

Name: glnA [H]

Synonym: Pden_4462

Alternate gene names: 119387167

Gene position: 1674857-1676263 (Clockwise)

Preceding gene: 119387166

Following gene: 119387168

Centisome position: 96.81

GC content: 63.18

Gene sequence:

>1407_bases
ATGACAGTCAAGGACGTTCTGGATCTGATGAAGGCCGAGGAAGTCGAATATGTCGACGTCCGCTTCACCGATCCCAAGGG
CAAGCTGCAGCATGTGACGCTGGTCGCCGATCTGGTGGACGAGGACTTCTTCGAGGAAGGCTTCATGTTCGACGGCTCCT
CGATCGCCGGCTGGAAATCCATCGACCAGTCCGACATGAAGCTGATGCCGGACGCATCCTCGGCCTATATCGACCCGTTC
TATGCCGAAAAGACGCTTTGCGTGCATTGCAACGTGGTCGAGCCCGACACCGGCGAGCCCTATGCCCGCGACCCGCGCGG
CACCGCGCTGAAGGCCGAGGCCTATCTGAAATCCTCGGGCATCGGCGACATCTCCTATTTCGGCCCCGAGGCGGAGTTCT
TCCTTTTCGACGACGTCCGCTATTCGGTGACGCCGCAGAAGGTCGGCTTCGAGATCGACTCGGTCGATGCCGCCTGGAAC
ACCGACACCGTCTACGAGGACGGCAACCTCGCCCATCGCGCGGCCCACAAGGGCGGCTATTTCCCGGTGAACCCGGTCGA
TGCGGCCCAGGACATCCGCGGCGAGATGCTCTCGACCATGAAGCGCATGGGGATCAAGGTCGACAAGCACCACCACGAGG
TCGCCACCGCCCAGCACGAGCTTGGCATGATCTTCGGCGGGCTGGTCGAGCAGGCCGACAACATCCAGAAATACAAATAC
GTCATCCACAACGTCGCCGCCGCCTATGGCAAGACCGCGACCTTCATGCCGAAGCCGATGAAGGGCGACAACGGCTCGGG
GATGCATGTGAACATGTCGATCTGGAAGGACGGCAAGCCGCTGTTCGCCGGCGACAAATATGCCGACCTGTCGCAAGAGG
CGCTGTGGTTCATCGGCGGCATCCTCAAGCACGCCAAGGCGCTCAATGCGCTGACCAACCCGGCGACCAACAGCTACAAG
CGCCTGATCCCCGGCTTCGAGGCCCCGGTGCTGCGCGCCTATTCGGCCCGCAACCGCTCGGGCTGCGTGCGGATCCCGTG
GACCGAGTCGCCGAAGGCCAAGCGCGTCGAGGCCCGTTTCCCCGATCCGGCCGCGAACCCCTACCTGGCCTTTGCCGCGC
TGCTGATGGCGGGCCTCGACGGCATCAAGAACAAGATCGACCCGGGCCCGGCCTCGGACAAGGACCTCTACGACCTGCCG
CCCGAGGAACTGGCCGAGATCCCGACCGTCTGCGGCTCGCTGCGCGAGGCGCTGGAGGAGCTGGAGAAGGACATGGACTT
CCTGCTGGCCGGCGACGTCTTCACCCGCGACCAGCTGGAAGGCTACATCAAGCTGAAATGGGAAGAGGTCTATGCCTACG
AGCATACCCCGCACCCCATCGAGTACGCGATGTATTACAGCTGCTGA

Upstream 100 bases:

>100_bases
AGACCGGCGACGACGCGGTCTGATCCCGCCCACCCGCACCATGAACCAATGACCCATGCCCGGCGGCGGCGCCGGGCAGA
CTGATGGAGGAAGGATCCCA

Downstream 100 bases:

>100_bases
TCCACGGCAGGGACAGTGACACGGGAAAGCCGCCCCACGGGGCGGCTTTCCCTTTGGGCGGGGCCTTTCGGCGCGAAGGG
GGCCTTCCCTCGCGATCCGC

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTVKDVLDLMKAEEVEYVDVRFTDPKGKLQHVTLVADLVDEDFFEEGFMFDGSSIAGWKSIDQSDMKLMPDASSAYIDPF
YAEKTLCVHCNVVEPDTGEPYARDPRGTALKAEAYLKSSGIGDISYFGPEAEFFLFDDVRYSVTPQKVGFEIDSVDAAWN
TDTVYEDGNLAHRAAHKGGYFPVNPVDAAQDIRGEMLSTMKRMGIKVDKHHHEVATAQHELGMIFGGLVEQADNIQKYKY
VIHNVAAAYGKTATFMPKPMKGDNGSGMHVNMSIWKDGKPLFAGDKYADLSQEALWFIGGILKHAKALNALTNPATNSYK
RLIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARFPDPAANPYLAFAALLMAGLDGIKNKIDPGPASDKDLYDLP
PEELAEIPTVCGSLREALEELEKDMDFLLAGDVFTRDQLEGYIKLKWEEVYAYEHTPHPIEYAMYYSC

Sequences:

>Translated_468_residues
MTVKDVLDLMKAEEVEYVDVRFTDPKGKLQHVTLVADLVDEDFFEEGFMFDGSSIAGWKSIDQSDMKLMPDASSAYIDPF
YAEKTLCVHCNVVEPDTGEPYARDPRGTALKAEAYLKSSGIGDISYFGPEAEFFLFDDVRYSVTPQKVGFEIDSVDAAWN
TDTVYEDGNLAHRAAHKGGYFPVNPVDAAQDIRGEMLSTMKRMGIKVDKHHHEVATAQHELGMIFGGLVEQADNIQKYKY
VIHNVAAAYGKTATFMPKPMKGDNGSGMHVNMSIWKDGKPLFAGDKYADLSQEALWFIGGILKHAKALNALTNPATNSYK
RLIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARFPDPAANPYLAFAALLMAGLDGIKNKIDPGPASDKDLYDLP
PEELAEIPTVCGSLREALEELEKDMDFLLAGDVFTRDQLEGYIKLKWEEVYAYEHTPHPIEYAMYYSC
>Mature_467_residues
TVKDVLDLMKAEEVEYVDVRFTDPKGKLQHVTLVADLVDEDFFEEGFMFDGSSIAGWKSIDQSDMKLMPDASSAYIDPFY
AEKTLCVHCNVVEPDTGEPYARDPRGTALKAEAYLKSSGIGDISYFGPEAEFFLFDDVRYSVTPQKVGFEIDSVDAAWNT
DTVYEDGNLAHRAAHKGGYFPVNPVDAAQDIRGEMLSTMKRMGIKVDKHHHEVATAQHELGMIFGGLVEQADNIQKYKYV
IHNVAAAYGKTATFMPKPMKGDNGSGMHVNMSIWKDGKPLFAGDKYADLSQEALWFIGGILKHAKALNALTNPATNSYKR
LIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARFPDPAANPYLAFAALLMAGLDGIKNKIDPGPASDKDLYDLPP
EELAEIPTVCGSLREALEELEKDMDFLLAGDVFTRDQLEGYIKLKWEEVYAYEHTPHPIEYAMYYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=387, Percent_Identity=26.6149870801034, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=62.046908315565, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI87081870, Length=392, Percent_Identity=25.2551020408163, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52137; Mature: 52006

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKDVLDLMKAEEVEYVDVRFTDPKGKLQHVTLVADLVDEDFFEEGFMFDGSSIAGWKS
CCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEHHHHHHHHHHHHCCCEECCCCCCCCCC
IDQSDMKLMPDASSAYIDPFYAEKTLCVHCNVVEPDTGEPYARDPRGTALKAEAYLKSSG
CCCCCCEECCCCCCCCCCCHHCCCEEEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHCCC
IGDISYFGPEAEFFLFDDVRYSVTPQKVGFEIDSVDAAWNTDTVYEDGNLAHRAAHKGGY
CCCEEECCCCCCEEEEECCEEEECHHHHCCEEECCCCCCCCCCEEECCCHHHHHHHCCCC
FPVNPVDAAQDIRGEMLSTMKRMGIKVDKHHHEVATAQHELGMIFGGLVEQADNIQKYKY
CCCCCCCHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIHNVAAAYGKTATFMPKPMKGDNGSGMHVNMSIWKDGKPLFAGDKYADLSQEALWFIGG
HHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
ILKHAKALNALTNPATNSYKRLIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARF
HHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEECCC
PDPAANPYLAFAALLMAGLDGIKNKIDPGPASDKDLYDLPPEELAEIPTVCGSLREALEE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEKDMDFLLAGDVFTRDQLEGYIKLKWEEVYAYEHTPHPIEYAMYYSC
HHHCHHEEEECCCHHHHHCCCEEEEEEEEEEEECCCCCCEEEEEEECC
>Mature Secondary Structure 
TVKDVLDLMKAEEVEYVDVRFTDPKGKLQHVTLVADLVDEDFFEEGFMFDGSSIAGWKS
CHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEHHHHHHHHHHHHCCCEECCCCCCCCCC
IDQSDMKLMPDASSAYIDPFYAEKTLCVHCNVVEPDTGEPYARDPRGTALKAEAYLKSSG
CCCCCCEECCCCCCCCCCCHHCCCEEEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHCCC
IGDISYFGPEAEFFLFDDVRYSVTPQKVGFEIDSVDAAWNTDTVYEDGNLAHRAAHKGGY
CCCEEECCCCCCEEEEECCEEEECHHHHCCEEECCCCCCCCCCEEECCCHHHHHHHCCCC
FPVNPVDAAQDIRGEMLSTMKRMGIKVDKHHHEVATAQHELGMIFGGLVEQADNIQKYKY
CCCCCCCHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIHNVAAAYGKTATFMPKPMKGDNGSGMHVNMSIWKDGKPLFAGDKYADLSQEALWFIGG
HHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
ILKHAKALNALTNPATNSYKRLIPGFEAPVLRAYSARNRSGCVRIPWTESPKAKRVEARF
HHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEECCC
PDPAANPYLAFAALLMAGLDGIKNKIDPGPASDKDLYDLPPEELAEIPTVCGSLREALEE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LEKDMDFLLAGDVFTRDQLEGYIKLKWEEVYAYEHTPHPIEYAMYYSC
HHHCHHEEEECCCHHHHHCCCEEEEEEEEEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7921264 [H]