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The map label for this gene is purB [H]

Identifier: 119387168

GI number: 119387168

Start: 1676470

End: 1677786

Strand: Direct

Name: purB [H]

Synonym: Pden_4463

Alternate gene names: 119387168

Gene position: 1676470-1677786 (Clockwise)

Preceding gene: 119387167

Following gene: 119387169

Centisome position: 96.9

GC content: 65.98

Gene sequence:

>1317_bases
ATGATCCCGCGCTATGCCCGCCCCGAAATGACCGCCATCTGGTCGCCCGAGACCAGGTTCCGGATCTGGTATGAGATCGA
GGCCCATGCCTGCGACGCCCAGGCCGACCTGGGCGTGATCCCGCGCGAGAACGCCGAAGCGGTCTGGAAGGCCAGGGACG
TCGAGTTCGACGTCGCCCGCATCGACGAGATCGAGGCCGTGACCAAGCACGACGTCATCGCCTTCCTGACCCATCTGGCC
GAGCATATCGGCGCCGAAGAGGCGCGCTTCGTCCATCAGGGCATGACCAGCTCGGACGTGCTGGACACCACGCTGAACGT
CCAGCTGGTGCGGGCCGCCGACATCCTGCTGGCCGACATGGACAAGGTGCTGGCCGCGCTGAAGAAACGCGCCTACGAGC
ACAAGAACACCGTCCGCATCGGCCGCAGCCACGGCATCCATGCCGAGCCGACCACCATGGGCCTGACCTTCGCCCGCTTC
TACGCCGAGATGGCGCGCGGCCGCGAACGGCTGGAACGCGCCCGCGACGAGGTCGCGACCGGCGCCATCTCGGGCGCGGT
CGGCACCTTCGCCAATATCGACCCCCGCGTCGAGGAACATGTCTGCGCGAAGATGGGCCTCTCCCCCGAACCGATCAGCA
CCCAGGTCATCCCGCGCGACCGCCACGCGATGTTCTTTGCCACGCTGGGCGTCATCGCCAGCTCGATCGAGAACATCGCC
ATCGAGATCCGCCACATGCAGCGCACCGAAGTGCTGGAGGCCGAGGAATATTTCAGCCCCGGCCAGAAGGGCAGCTCGGC
CATGCCCCACAAGCGCAACCCGGTGCTGACCGAGAACCTGACCGGCCTCGCCCGCCTGGTGCGCATGGCGGTGGTCCCGG
CGATGGAGAACGTGGCGCTGTGGCATGAACGCGACATCAGCCATTCCTCGGTCGAACGCGGCATCGCGCCGGACGCGACG
ATCACCCTGGACTTCGCCCTGAACCGCCTGGCGGGCGTGATCGAAAAGCTGGTGGTCTACCCCGAGAACATGCTGAAGAA
CATGAACAAGTTCAAGGGCCTGGTCATGTCCCAGCGCGTGCTTCTGGCCCTGACCCAGGCCGGCGTCAGCCGCGAGGACG
CCTATCGCCTGGTGCAGCGCAACGCCATGAAGGTCTGGGAACAGGGCGCCGACTTCAAGACCGAGCTGCTGGCCGATGCC
GAGGTGACGGCCGCGCTTTCGCCCGCCGAGATCGAGGAGAAATTCGACCTGGGCTATCACACCAAGCATGTGGACACGAT
TTTCGCCCGGGTCTTCGGCGAAAACGGCGCGCATTAA

Upstream 100 bases:

>100_bases
GATGCCGCGCCCGGCAAGGGCCTCTGCCCCGCGATGATGCCGGCGCGGTTGCGGGACGTGAGGCAAGCGACTAGAACCCC
CGCAAACACAGGAGCCCCCG

Downstream 100 bases:

>100_bases
CCGCCTGTTCGCTTTTCCCTGCCAGATTGCCCCCGGAAAGTTGGGAATCGGGGGCAATCCATGGGTGTTCCGGCGCTCGG
GGCGACGGCGGGGCTCAGTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MIPRYARPEMTAIWSPETRFRIWYEIEAHACDAQADLGVIPRENAEAVWKARDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHIGAEEARFVHQGMTSSDVLDTTLNVQLVRAADILLADMDKVLAALKKRAYEHKNTVRIGRSHGIHAEPTTMGLTFARF
YAEMARGRERLERARDEVATGAISGAVGTFANIDPRVEEHVCAKMGLSPEPISTQVIPRDRHAMFFATLGVIASSIENIA
IEIRHMQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVALWHERDISHSSVERGIAPDAT
ITLDFALNRLAGVIEKLVVYPENMLKNMNKFKGLVMSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQGADFKTELLADA
EVTAALSPAEIEEKFDLGYHTKHVDTIFARVFGENGAH

Sequences:

>Translated_438_residues
MIPRYARPEMTAIWSPETRFRIWYEIEAHACDAQADLGVIPRENAEAVWKARDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHIGAEEARFVHQGMTSSDVLDTTLNVQLVRAADILLADMDKVLAALKKRAYEHKNTVRIGRSHGIHAEPTTMGLTFARF
YAEMARGRERLERARDEVATGAISGAVGTFANIDPRVEEHVCAKMGLSPEPISTQVIPRDRHAMFFATLGVIASSIENIA
IEIRHMQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVALWHERDISHSSVERGIAPDAT
ITLDFALNRLAGVIEKLVVYPENMLKNMNKFKGLVMSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQGADFKTELLADA
EVTAALSPAEIEEKFDLGYHTKHVDTIFARVFGENGAH
>Mature_438_residues
MIPRYARPEMTAIWSPETRFRIWYEIEAHACDAQADLGVIPRENAEAVWKARDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHIGAEEARFVHQGMTSSDVLDTTLNVQLVRAADILLADMDKVLAALKKRAYEHKNTVRIGRSHGIHAEPTTMGLTFARF
YAEMARGRERLERARDEVATGAISGAVGTFANIDPRVEEHVCAKMGLSPEPISTQVIPRDRHAMFFATLGVIASSIENIA
IEIRHMQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVALWHERDISHSSVERGIAPDAT
ITLDFALNRLAGVIEKLVVYPENMLKNMNKFKGLVMSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQGADFKTELLADA
EVTAALSPAEIEEKFDLGYHTKHVDTIFARVFGENGAH

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=421, Percent_Identity=29.4536817102138, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=29.3963254593176, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI1787376, Length=318, Percent_Identity=27.3584905660377, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17508577, Length=409, Percent_Identity=26.161369193154, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=362, Percent_Identity=26.7955801104972, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323391, Length=407, Percent_Identity=28.2555282555283, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24647570, Length=409, Percent_Identity=25.9168704156479, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24640179, Length=207, Percent_Identity=29.951690821256, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640177, Length=207, Percent_Identity=29.951690821256, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78710009, Length=205, Percent_Identity=27.8048780487805, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48925; Mature: 48925

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYARPEMTAIWSPETRFRIWYEIEAHACDAQADLGVIPRENAEAVWKARDVEFDVAR
CCCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEHHHH
IDEIEAVTKHDVIAFLTHLAEHIGAEEARFVHQGMTSSDVLDTTLNVQLVRAADILLADM
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHCEEEHHHHHHHHHHH
DKVLAALKKRAYEHKNTVRIGRSHGIHAEPTTMGLTFARFYAEMARGRERLERARDEVAT
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEHVCAKMGLSPEPISTQVIPRDRHAMFFATLGVIASSIENIA
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IEIRHMQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERGIAPDATITLDFALNRLAGVIEKLVVYPENMLKNMNKFKGLVMSQRV
HHHCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMKVWEQGADFKTELLADAEVTAALSPAEIEEKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCHHHHHHHHCCCCC
TKHVDTIFARVFGENGAH
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIPRYARPEMTAIWSPETRFRIWYEIEAHACDAQADLGVIPRENAEAVWKARDVEFDVAR
CCCCCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCEEHHHH
IDEIEAVTKHDVIAFLTHLAEHIGAEEARFVHQGMTSSDVLDTTLNVQLVRAADILLADM
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHCEEEHHHHHHHHHHH
DKVLAALKKRAYEHKNTVRIGRSHGIHAEPTTMGLTFARFYAEMARGRERLERARDEVAT
HHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEHVCAKMGLSPEPISTQVIPRDRHAMFFATLGVIASSIENIA
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IEIRHMQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERGIAPDATITLDFALNRLAGVIEKLVVYPENMLKNMNKFKGLVMSQRV
HHHCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMKVWEQGADFKTELLADAEVTAALSPAEIEEKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHCCCHHHHHHHHCCCCC
TKHVDTIFARVFGENGAH
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]