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The map label for this gene is leuA [H]

Identifier: 119386737

GI number: 119386737

Start: 1178755

End: 1180317

Strand: Direct

Name: leuA [H]

Synonym: Pden_4030

Alternate gene names: 119386737

Gene position: 1178755-1180317 (Clockwise)

Preceding gene: 119386736

Following gene: 119386739

Centisome position: 68.13

GC content: 64.88

Gene sequence:

>1563_bases
ATGACCGATCCGAACCGCGTATTGATCTTTGACACCACCCTGCGCGACGGCGAGCAATCGCCCGGCGCCACCATGAGCCA
TACCGAAAAGCTGGACATCGCCGAGATGCTAGACGAGATGGGCGTCGATATCATCGAGGCCGGTTTCCCCATCGCCTCGG
AAGGCGATTTCACCGCCGTCTCCGAGATCGCCAGGCGCGCCAAGAACTCGGTGATCTGTGGCCTGGCACGGGCGCAGTTT
CCCGATATCGACCGCTGCTGGGAGGCGGTGAAGCATGCGCAGAAGCCGCGCATCCACACCTTCATCGGCACCTCGCCGCT
GCACCGCGCCATCCCCAACCTGGACATGGACCAGATGGCCGAGCGCATCCACGACACCGTGACCCATGCCCGCAACCTGT
GCGACAACGTGCAATGGTCGGCCATGGACGCCACCCGGACCGAGCACGACTATCTGTGCCGCGTGGTCGAGATCGCGATC
AAATGCGGCGCGACCACCATCAACATCCCCGATACCGTCGGCTATACTGCGCCGCGCGAATCCGCCGCGCTGATCCGCAT
GCTGCTGGAACGGGTGCCGGGCGCCGATACCGTCGTCTTCGCCACGCATTGCCACAACGACCTGGGCATGGCGACGGCGA
ACTCTCTGGCCGCGGTCGAGGCGGGTGCGCGCCAGGTCGAATGCACCATCAACGGCCTGGGCGAACGCGCCGGCAACACG
GCGCTGGAGGAGGTGGTGATGGCGCTGAAGGTGCGCCACGACATCATGCCCTATACGACCGGCATCGACACCACCAAGAT
CATGGGCCTGTCGCGGCGCGTTTCGCAAGCCTCGGGCTTCCTGGTGCAACCGAACAAGGCGGTGGTCGGCAAGAACGCCT
TCCTGCACGAATCCGGCATCCACCAGGACGGCGTGCTGAAGAATGTCGAGACCTTCGAGATCATGAAGCCCGCCGATATC
GGCCTGAACGAGGCCAATATCGCCATGGGCAAGCATTCCGGCCGCGCCGCGCTGCGCGCCAAGCTGAAGGATCTGGGCTA
TGAGCTGGGCGACAACCAGCTGAAGGATGTCTTCATCCGCTTCAAGGCGCTGGCCGACCGCAAGAAGGAGGTCTATGACG
ACGACCTGGTCGCGCTGGTCCAGGATGCCGCGACGAATACCGAGGACGACCATCTGCAGGTCAAGCATCTGCGCGTCGTC
TGCGGCAGCGACGGGCAATCGGCCGACCTGACCATGCTGGTCGGCGGCGAGGAGCAGACCGTCCATGCCGAGGGCGACGG
TCCGGTCGATGCCTGCTTCAACGCCGTCAAGGCGATCTGGCCGCATCGGGCGCGTCTGCAGCTCTATCAGGTCCATGCCG
TGACCGAGGGCACCGATGCCCAGGCCACCGTCAGCGTGCGCATGGAGGAAGAGGGCCGCATCGCCACCGGCCAGGCCTCG
GATACCGACACCATCCTGGCCAGCGTCAAGGCCTATGTCGGGGCGCTGAACCGGCTGAAGGTGCGCCGCGACATGGTCGG
CGAAGGCGCCGATGCCAAGCAGGTCAGCATGTATTCGCACTGA

Upstream 100 bases:

>100_bases
CGGTCTCCTTCTTCTTACGCTGCGCTGAGCGGGTCTCCGGGCCGCCGCTCAGTCAGGTTCGTGCCCGGACCCCTGCGAGC
ATGAAGAAGGATTCCCCAAA

Downstream 100 bases:

>100_bases
TCCCCTGCGAAACCACGGACAAGGGGCGGCGCTCAGGCCGCCCCTTTGCGTTCCACGAATCCAAGGTCGATGCCTTGCAG
GAAGGGGAAGTCCTCGGCCA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MTDPNRVLIFDTTLRDGEQSPGATMSHTEKLDIAEMLDEMGVDIIEAGFPIASEGDFTAVSEIARRAKNSVICGLARAQF
PDIDRCWEAVKHAQKPRIHTFIGTSPLHRAIPNLDMDQMAERIHDTVTHARNLCDNVQWSAMDATRTEHDYLCRVVEIAI
KCGATTINIPDTVGYTAPRESAALIRMLLERVPGADTVVFATHCHNDLGMATANSLAAVEAGARQVECTINGLGERAGNT
ALEEVVMALKVRHDIMPYTTGIDTTKIMGLSRRVSQASGFLVQPNKAVVGKNAFLHESGIHQDGVLKNVETFEIMKPADI
GLNEANIAMGKHSGRAALRAKLKDLGYELGDNQLKDVFIRFKALADRKKEVYDDDLVALVQDAATNTEDDHLQVKHLRVV
CGSDGQSADLTMLVGGEEQTVHAEGDGPVDACFNAVKAIWPHRARLQLYQVHAVTEGTDAQATVSVRMEEEGRIATGQAS
DTDTILASVKAYVGALNRLKVRRDMVGEGADAKQVSMYSH

Sequences:

>Translated_520_residues
MTDPNRVLIFDTTLRDGEQSPGATMSHTEKLDIAEMLDEMGVDIIEAGFPIASEGDFTAVSEIARRAKNSVICGLARAQF
PDIDRCWEAVKHAQKPRIHTFIGTSPLHRAIPNLDMDQMAERIHDTVTHARNLCDNVQWSAMDATRTEHDYLCRVVEIAI
KCGATTINIPDTVGYTAPRESAALIRMLLERVPGADTVVFATHCHNDLGMATANSLAAVEAGARQVECTINGLGERAGNT
ALEEVVMALKVRHDIMPYTTGIDTTKIMGLSRRVSQASGFLVQPNKAVVGKNAFLHESGIHQDGVLKNVETFEIMKPADI
GLNEANIAMGKHSGRAALRAKLKDLGYELGDNQLKDVFIRFKALADRKKEVYDDDLVALVQDAATNTEDDHLQVKHLRVV
CGSDGQSADLTMLVGGEEQTVHAEGDGPVDACFNAVKAIWPHRARLQLYQVHAVTEGTDAQATVSVRMEEEGRIATGQAS
DTDTILASVKAYVGALNRLKVRRDMVGEGADAKQVSMYSH
>Mature_519_residues
TDPNRVLIFDTTLRDGEQSPGATMSHTEKLDIAEMLDEMGVDIIEAGFPIASEGDFTAVSEIARRAKNSVICGLARAQFP
DIDRCWEAVKHAQKPRIHTFIGTSPLHRAIPNLDMDQMAERIHDTVTHARNLCDNVQWSAMDATRTEHDYLCRVVEIAIK
CGATTINIPDTVGYTAPRESAALIRMLLERVPGADTVVFATHCHNDLGMATANSLAAVEAGARQVECTINGLGERAGNTA
LEEVVMALKVRHDIMPYTTGIDTTKIMGLSRRVSQASGFLVQPNKAVVGKNAFLHESGIHQDGVLKNVETFEIMKPADIG
LNEANIAMGKHSGRAALRAKLKDLGYELGDNQLKDVFIRFKALADRKKEVYDDDLVALVQDAATNTEDDHLQVKHLRVVC
GSDGQSADLTMLVGGEEQTVHAEGDGPVDACFNAVKAIWPHRARLQLYQVHAVTEGTDAQATVSVRMEEEGRIATGQASD
TDTILASVKAYVGALNRLKVRRDMVGEGADAKQVSMYSH

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=46.9758064516129, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6320019, Length=378, Percent_Identity=30.1587301587302, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=29.8941798941799, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324682, Length=542, Percent_Identity=24.9077490774908, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=538, Percent_Identity=24.907063197026, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56629; Mature: 56498

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPNRVLIFDTTLRDGEQSPGATMSHTEKLDIAEMLDEMGVDIIEAGFPIASEGDFTAV
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCCCCCCCHHHH
SEIARRAKNSVICGLARAQFPDIDRCWEAVKHAQKPRIHTFIGTSPLHRAIPNLDMDQMA
HHHHHHHCCCEEEEEHHCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHHHHH
ERIHDTVTHARNLCDNVQWSAMDATRTEHDYLCRVVEIAIKCGATTINIPDTVGYTAPRE
HHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCH
SAALIRMLLERVPGADTVVFATHCHNDLGMATANSLAAVEAGARQVECTINGLGERAGNT
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCHHHHHHCCCEEEEEEEECCCCCCCCH
ALEEVVMALKVRHDIMPYTTGIDTTKIMGLSRRVSQASGFLVQPNKAVVGKNAFLHESGI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCEEECCCCEEECCCC
HQDGVLKNVETFEIMKPADIGLNEANIAMGKHSGRAALRAKLKDLGYELGDNQLKDVFIR
CCCCCCCCCCHHEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
FKALADRKKEVYDDDLVALVQDAATNTEDDHLQVKHLRVVCGSDGQSADLTMLVGGEEQT
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCEEEEECCCCCE
VHAEGDGPVDACFNAVKAIWPHRARLQLYQVHAVTEGTDAQATVSVRMEEEGRIATGQAS
EEECCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCEEEEEEEEECCCCEECCCCC
DTDTILASVKAYVGALNRLKVRRDMVGEGADAKQVSMYSH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDPNRVLIFDTTLRDGEQSPGATMSHTEKLDIAEMLDEMGVDIIEAGFPIASEGDFTAV
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCCCCCCCHHHH
SEIARRAKNSVICGLARAQFPDIDRCWEAVKHAQKPRIHTFIGTSPLHRAIPNLDMDQMA
HHHHHHHCCCEEEEEHHCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHHHHH
ERIHDTVTHARNLCDNVQWSAMDATRTEHDYLCRVVEIAIKCGATTINIPDTVGYTAPRE
HHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCH
SAALIRMLLERVPGADTVVFATHCHNDLGMATANSLAAVEAGARQVECTINGLGERAGNT
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCHHHHHHCCCEEEEEEEECCCCCCCCH
ALEEVVMALKVRHDIMPYTTGIDTTKIMGLSRRVSQASGFLVQPNKAVVGKNAFLHESGI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCEEECCCCEEECCCC
HQDGVLKNVETFEIMKPADIGLNEANIAMGKHSGRAALRAKLKDLGYELGDNQLKDVFIR
CCCCCCCCCCHHEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
FKALADRKKEVYDDDLVALVQDAATNTEDDHLQVKHLRVVCGSDGQSADLTMLVGGEEQT
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCEEEEECCCCCE
VHAEGDGPVDACFNAVKAIWPHRARLQLYQVHAVTEGTDAQATVSVRMEEEGRIATGQAS
EEECCCCCHHHHHHHHHHHCCCCHHEEEEEEEEECCCCCCEEEEEEEEECCCCEECCCCC
DTDTILASVKAYVGALNRLKVRRDMVGEGADAKQVSMYSH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA