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The map label for this gene is garR [C]

Identifier: 119386710

GI number: 119386710

Start: 1144811

End: 1145677

Strand: Direct

Name: garR [C]

Synonym: Pden_4003

Alternate gene names: 119386710

Gene position: 1144811-1145677 (Clockwise)

Preceding gene: 119386707

Following gene: 119386713

Centisome position: 66.17

GC content: 69.55

Gene sequence:

>867_bases
ATGGCGCGCGTGGCATTTCTGGGACTTGGGGTGATGGGCTTTCCCATGGCGGGGCACCTGGCCGCGGCCGGGCATGAGGT
GACGGTCTGGAACCGCAGCCCCGCCAAGGCCGAGGCCTGGGCCGCCCGTCACAAGGGCCGCGTGGCCGCCACCGCGCGCG
AAGCGGCCGAGGGTGCCGAGTTCGTCATGGCCTGCGTCGGCAATGACGACGACCTGCGCCAGGTCTGCCTGGGCGAGGCG
GGTGCCTTTGCCGGCATGGGCAAGGGCGCGGTCTTCGTCGACCACACCACCGTTTCGGCGGCAGTGACGCGCCAGCTTTC
TGCGCTGGCGGTCGAGGCCGGGCTGGGCTTTGTCGATGCCCCGGTCTCGGGCGGGCAGGCGGGGGCCGAGAGCGGCCAGC
TTTCCGTGATGTGCGGCGGCACGCAGGACGATTACGCCCGGGCCGAGTCGGTGATCGCCGCCTATGCCAAGATCTGCAAG
CTCATGGGGCCCTCGGGTGCCGGGCAACTGGCCAAGATGTGCAACCAGATCGCCATCGCCGGGCTGGTGCAGGGCCTGTC
GGAATCGCTGCATTTCGCCGAAAAGGCCGGGCTGTCGATCCCCGAGGTGGTCGAGGTGATCAGCCAGGGCGCCGCCGGCA
GCTGGCAGATGCAGAACCGTCACCAGACCATGGCCGAGAACCACTTCGACTTCGGCTTTGCGGTGGACTGGATGCGCAAG
GACCTGGGCATCTGCCTGGCCACCGCCGACGAAACCGGCGCCAGCCTGCCGGTGACGGCGCTGGTCGACCAGTTCTACAA
GGAAGTGCAGGCCATGGGCGGCGGGCGCTGGGACACCTCCTCGCTGATCGCAAGGCTGCGCCGGTGA

Upstream 100 bases:

>100_bases
AGAGGTTGACCCCCGCGCCGGGTGATGAAATTCATCGGGGTGAATAGGGCAGGGCGCGGGCCGGATCAACGCGCCTTTAC
GACATGAGGGAGAGAATCTG

Downstream 100 bases:

>100_bases
CGCCCATGCTTGCCAAGGGTGCGGCGATCGCGCTATCAGCCGGCATGGGGGCGGTAGCTCAGCGGTAGAGCGACACGTTT
ACACCGTGTTGGTCGGGGGT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MARVAFLGLGVMGFPMAGHLAAAGHEVTVWNRSPAKAEAWAARHKGRVAATAREAAEGAEFVMACVGNDDDLRQVCLGEA
GAFAGMGKGAVFVDHTTVSAAVTRQLSALAVEAGLGFVDAPVSGGQAGAESGQLSVMCGGTQDDYARAESVIAAYAKICK
LMGPSGAGQLAKMCNQIAIAGLVQGLSESLHFAEKAGLSIPEVVEVISQGAAGSWQMQNRHQTMAENHFDFGFAVDWMRK
DLGICLATADETGASLPVTALVDQFYKEVQAMGGGRWDTSSLIARLRR

Sequences:

>Translated_288_residues
MARVAFLGLGVMGFPMAGHLAAAGHEVTVWNRSPAKAEAWAARHKGRVAATAREAAEGAEFVMACVGNDDDLRQVCLGEA
GAFAGMGKGAVFVDHTTVSAAVTRQLSALAVEAGLGFVDAPVSGGQAGAESGQLSVMCGGTQDDYARAESVIAAYAKICK
LMGPSGAGQLAKMCNQIAIAGLVQGLSESLHFAEKAGLSIPEVVEVISQGAAGSWQMQNRHQTMAENHFDFGFAVDWMRK
DLGICLATADETGASLPVTALVDQFYKEVQAMGGGRWDTSSLIARLRR
>Mature_287_residues
ARVAFLGLGVMGFPMAGHLAAAGHEVTVWNRSPAKAEAWAARHKGRVAATAREAAEGAEFVMACVGNDDDLRQVCLGEAG
AFAGMGKGAVFVDHTTVSAAVTRQLSALAVEAGLGFVDAPVSGGQAGAESGQLSVMCGGTQDDYARAESVIAAYAKICKL
MGPSGAGQLAKMCNQIAIAGLVQGLSESLHFAEKAGLSIPEVVEVISQGAAGSWQMQNRHQTMAENHFDFGFAVDWMRKD
LGICLATADETGASLPVTALVDQFYKEVQAMGGGRWDTSSLIARLRR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=28.7719298245614, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=27.0547945205479, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI145693186, Length=281, Percent_Identity=34.5195729537367, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=33.2167832167832, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=31.1643835616438, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789092, Length=294, Percent_Identity=25.5102040816327, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=25.8181818181818, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=29.0102389078498, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=29.0102389078498, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=22.9681978798587, Blast_Score=103, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 29947; Mature: 29816

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARVAFLGLGVMGFPMAGHLAAAGHEVTVWNRSPAKAEAWAARHKGRVAATAREAAEGAE
CCEEEEECCCHHHCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCHH
FVMACVGNDDDLRQVCLGEAGAFAGMGKGAVFVDHTTVSAAVTRQLSALAVEAGLGFVDA
EEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEC
PVSGGQAGAESGQLSVMCGGTQDDYARAESVIAAYAKICKLMGPSGAGQLAKMCNQIAIA
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLVQGLSESLHFAEKAGLSIPEVVEVISQGAAGSWQMQNRHQTMAENHFDFGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
DLGICLATADETGASLPVTALVDQFYKEVQAMGGGRWDTSSLIARLRR
HHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
ARVAFLGLGVMGFPMAGHLAAAGHEVTVWNRSPAKAEAWAARHKGRVAATAREAAEGAE
CEEEEECCCHHHCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCHH
FVMACVGNDDDLRQVCLGEAGAFAGMGKGAVFVDHTTVSAAVTRQLSALAVEAGLGFVDA
EEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEC
PVSGGQAGAESGQLSVMCGGTQDDYARAESVIAAYAKICKLMGPSGAGQLAKMCNQIAIA
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
GLVQGLSESLHFAEKAGLSIPEVVEVISQGAAGSWQMQNRHQTMAENHFDFGFAVDWMRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
DLGICLATADETGASLPVTALVDQFYKEVQAMGGGRWDTSSLIARLRR
HHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA