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The map label for this gene is gshB [H]

Identifier: 119386667

GI number: 119386667

Start: 1097346

End: 1098296

Strand: Direct

Name: gshB [H]

Synonym: Pden_3960

Alternate gene names: 119386667

Gene position: 1097346-1098296 (Clockwise)

Preceding gene: 119386666

Following gene: 119386669

Centisome position: 63.43

GC content: 63.62

Gene sequence:

>951_bases
ATGAGTCTTTACGTGGCCCTGCAGATGGACCCGATCGAGCAGGTCTCGATCGACGGCGACAGCACGTTCCGGCTGGGGCT
TGAGGCCGAAGCCCGCGGCCACCGGCTGTTCCAGTATACAGCCGACCGCCTGAGCTATTGCGAAGGGCGGGTCATGGCGC
GCGGCCGGCCGATCACCCTGCGCCGCGAACAGGGCAACCATGTGAGTTTCGGCGACTGGACCGATGCCGATCTGTCGGAG
TTCGACGTGGTCTGGCTGCGGCAGGATCCGCCCTTCGACATGGGCTACATCACCACCACGCACCTGCTGGACCGGGTGCA
GCCCTCGACCTATGTCGTCAACGATCCGTTCTGGGTGCGCAACAGTCCCGAGAAGCTGCTGGTGCTCGATTTCCCAGAGC
TTACACCGCCCACGCTGATCACCCGCGATCTGGCACAGATACGTGCCTTTCGCGAGCGGCATGGCGACTTCATCCTCAAG
CCGCTCTACGGCAATGGCGGGGTCGGCATCTTCCATATGCGACCCGACGACCCGAATCTGTCCTCGCTGGTCGAGACTTT
CCTGGCCAATAGTCGCGAGCCGATCATCGCCCAGAAATACCTGCCCGCCGTGGTCAAGGGCGACAAGCGCATCATCCTGG
TCGATGGCGAGCCGGTCGGCGCGATCAACCGGGTTCCGGCCGAGGGCGAGGCACGGTCGAACATGCATGTCGGCGGCCGT
CCCGAAAAGATCGGCCTGACCGAGCGCGAATACGAGATCTGCGCCCATATCGGTCCGGTCCTGCGCGAAAAGGGCCTGCT
TTTCACTGGCATAGATGTGATCGACGGCTGGCTGACCGAGATCAACGTGACCTCGCCGACCGGCATTCAGGAACTCGAGC
GCTTCGATGGCATCAATGCGGCTGCGCTGATCTGGGACGCGATCGAGCGCCGGCTGGCCGAGCGAAGCTGA

Upstream 100 bases:

>100_bases
TGGGCAGAAACACGAATCTCGGCGCCGATCCGGCGCTGCCGCTTGCAAAGCCCGTCCATGCGCGCGAGAACTTCGGCCGA
AACAGGCTGGGGATGATGGT

Downstream 100 bases:

>100_bases
TACCGCACCGGAAGGCGAAGCCGAAGGCAGGGCTCCGGGGATCCAATGATCCGCTGCTGCGGGTTCATGGAGGGCCAGCC
TTGCCGCGACCTCGGCCGCA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSLYVALQMDPIEQVSIDGDSTFRLGLEAEARGHRLFQYTADRLSYCEGRVMARGRPITLRREQGNHVSFGDWTDADLSE
FDVVWLRQDPPFDMGYITTTHLLDRVQPSTYVVNDPFWVRNSPEKLLVLDFPELTPPTLITRDLAQIRAFRERHGDFILK
PLYGNGGVGIFHMRPDDPNLSSLVETFLANSREPIIAQKYLPAVVKGDKRIILVDGEPVGAINRVPAEGEARSNMHVGGR
PEKIGLTEREYEICAHIGPVLREKGLLFTGIDVIDGWLTEINVTSPTGIQELERFDGINAAALIWDAIERRLAERS

Sequences:

>Translated_316_residues
MSLYVALQMDPIEQVSIDGDSTFRLGLEAEARGHRLFQYTADRLSYCEGRVMARGRPITLRREQGNHVSFGDWTDADLSE
FDVVWLRQDPPFDMGYITTTHLLDRVQPSTYVVNDPFWVRNSPEKLLVLDFPELTPPTLITRDLAQIRAFRERHGDFILK
PLYGNGGVGIFHMRPDDPNLSSLVETFLANSREPIIAQKYLPAVVKGDKRIILVDGEPVGAINRVPAEGEARSNMHVGGR
PEKIGLTEREYEICAHIGPVLREKGLLFTGIDVIDGWLTEINVTSPTGIQELERFDGINAAALIWDAIERRLAERS
>Mature_315_residues
SLYVALQMDPIEQVSIDGDSTFRLGLEAEARGHRLFQYTADRLSYCEGRVMARGRPITLRREQGNHVSFGDWTDADLSEF
DVVWLRQDPPFDMGYITTTHLLDRVQPSTYVVNDPFWVRNSPEKLLVLDFPELTPPTLITRDLAQIRAFRERHGDFILKP
LYGNGGVGIFHMRPDDPNLSSLVETFLANSREPIIAQKYLPAVVKGDKRIILVDGEPVGAINRVPAEGEARSNMHVGGRP
EKIGLTEREYEICAHIGPVLREKGLLFTGIDVIDGWLTEINVTSPTGIQELERFDGINAAALIWDAIERRLAERS

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=315, Percent_Identity=42.5396825396825, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35622; Mature: 35491

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYVALQMDPIEQVSIDGDSTFRLGLEAEARGHRLFQYTADRLSYCEGRVMARGRPITL
CEEEEEEEECCCCEEEECCCCEEEEEEECCCCCCEEEEHHHHHHHHHCCEEEECCCCEEE
RREQGNHVSFGDWTDADLSEFDVVWLRQDPPFDMGYITTTHLLDRVQPSTYVVNDPFWVR
EECCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCEEEC
NSPEKLLVLDFPELTPPTLITRDLAQIRAFRERHGDFILKPLYGNGGVGIFHMRPDDPNL
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCH
SSLVETFLANSREPIIAQKYLPAVVKGDKRIILVDGEPVGAINRVPAEGEARSNMHVGGR
HHHHHHHHCCCCCCEEHHHHCHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PEKIGLTEREYEICAHIGPVLREKGLLFTGIDVIDGWLTEINVTSPTGIQELERFDGINA
CCCCCCCHHHHHHHHHHHHHHHHCCCEEECHHHHCCEEEEEEECCCCCHHHHHHHCCCCH
AALIWDAIERRLAERS
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLYVALQMDPIEQVSIDGDSTFRLGLEAEARGHRLFQYTADRLSYCEGRVMARGRPITL
EEEEEEEECCCCEEEECCCCEEEEEEECCCCCCEEEEHHHHHHHHHCCEEEECCCCEEE
RREQGNHVSFGDWTDADLSEFDVVWLRQDPPFDMGYITTTHLLDRVQPSTYVVNDPFWVR
EECCCCEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCEEEC
NSPEKLLVLDFPELTPPTLITRDLAQIRAFRERHGDFILKPLYGNGGVGIFHMRPDDPNL
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCH
SSLVETFLANSREPIIAQKYLPAVVKGDKRIILVDGEPVGAINRVPAEGEARSNMHVGGR
HHHHHHHHCCCCCCEEHHHHCHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PEKIGLTEREYEICAHIGPVLREKGLLFTGIDVIDGWLTEINVTSPTGIQELERFDGINA
CCCCCCCHHHHHHHHHHHHHHHHCCCEEECHHHHCCEEEEEEECCCCCHHHHHHHCCCCH
AALIWDAIERRLAERS
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]