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The map label for this gene is cysJ [H]

Identifier: 119386009

GI number: 119386009

Start: 454411

End: 456618

Strand: Direct

Name: cysJ [H]

Synonym: Pden_3290

Alternate gene names: 119386009

Gene position: 454411-456618 (Clockwise)

Preceding gene: 119386008

Following gene: 119386010

Centisome position: 26.27

GC content: 68.75

Gene sequence:

>2208_bases
ATGATCCGCATGCTGCATCGCTGGCCGGGTCTGCTGGCCCTTGCTCTCGTCATGGTCCTCGCGATCAGCGGGGCGGCGCT
CTCGGTCTTTCCAGTGGCCGAGCGGTTTTCCACGCCGCAGGCCGAGGCCGGCCTGACCGTTGCCGATCTCGCAGAACGCA
TCCGGACTGCCTATCCGGGGGTGGAGCAGATCCGGCGTGCGCCCTCGGGCCGGATTACTGCGTTCTGGTTCGACGACGGC
ACGCCCGGCGCGGCGGTGATCGACCCCGCGACCGGCAAGGGCATCGCCAGCGCCGATCCGAACCCGGTCGAACGCTGGCT
GACCAGCCTTCACCGCTCGCTGTTTCTTGGCGATGCCGGCCGTATCGTCATGGCCGCCGGTGCGGCGGCGATGCTGGTCC
TGTCGGTCTCCGGCGCGATGCTGGTCGCGCGGCGCATGGGCGGCTGGCGGCGCTGGTTCACCCCTTTGCGCGGGCCGGCA
TCGAGCCGCATCCATACCGGGATCGCCCGCGTGGCGGTCGCGGGGCTGATTCTGTCCTCGGCGACCGCACTGTGGATGGC
CGCCTCGACCTTCGATCTTCTGCCGGATGGCGCCGCACCGCCGGCCTTTCCGACCGAGGTCAGCGGCAGCACCGGGGTCT
CCCTTGCTGCGATGGAGCCGCTCTCCGTGATTCCTGTCACGGAGTTGCGCGAACTGAGCTTTCCCTATCCGGGCGACGCC
ACGGACGCCTTCACCCTGAAGACGGATCGCGGCACCGGCTATCTTGATCAGGGGACGGGTGAGCTTCTGGCTTGGGCCGA
GCTGACGATGTGGGAACGGATATCTGAAACCATCTACATGCTGCATACCGGACAGGGGGCCGCATCTCTCGGCCTCGTGC
TGGGTGTGATGGCGCTCGGCGTGCCGGCAATGGGGGTGAGTGGTCTTGTGCTCTGGTTCGCGGGGCGGCGGTCAAGGCCG
CGTCTCCGTGGCAATCATCCTGCAAAAGGCGCTGGGACCATCCTGCTGGTGGGCTCGGAGGGCGGCAGCACCTGGGGCTT
CGCTGCGACGCTCCAACATGCGCTGACCACGGTGGGCCAGCATGTCCATGTCGCGCCGATGTCTGGCTTCGATCCGGCGC
GCTATCCGCGGGCCGAACGGTTCATCGTCCTTTCCGCCACCTGGGGCGAGGGACAGGCTCCCGCCACGGCGAAGGGGTTC
ATCGAACGCCTCGCGGTGCTGGACTCCGCGCCCCGCGCCCCGCTTGCCGTGCTGGGTTTCGGTGATCGCAGCTTCCCCGC
GTTCTGCGCGTTCGCCGATGAAGTCGCGCGGCTGGCTGCTGCCAAAGGCTGGGCGCAATTGATGCCCTTCGACACGGTCG
ATCGCCAGTCGCCGCAGGATTTCGCCCGCTGGGGCCGCGCCCTGGGGGCGGCGCTGGGGATCGAGCTTGCGCTCGAACAT
GTGCCAACCCCGCCGGTGACGGAGCGGCTCACCCTGATCTCGCGCCGTGATTACGGGGCCGAGGTGCAGGCGCCGACCAC
GATCCTGCGCTTTGCCCTACCCGAGGCGACGTTTTTGCAACGGGTGATAGCTCGGGGGTTTGGCCGGTTTCAAGCCGGTG
ATCTCCTCGGCGTTCTGCCCGAGGGTGCGGACCTGCCGCGCTTCTACTCGCTGGCGTCCGGACGGCGCGACGGATTCGTC
GAGATCGTGGTGCGCAAGCATCCCGGCGGCCTCTGCTCGGGTCAGCTTCTGGCGCTGGAGCCGGGCCAGGCGATCCGCGC
CTTCGTCCGGCACAATCCGGCTTTCCATGCCGCAGCAGGACGCTCGCCGCTGATCCTGATCGGCGCTGGCACCGGCATCG
GCCCGCTTGCGGGCTTCATCCGGGGCAACAGCCGCAAGCGGCCCATCCATCTGGCCTTCGGGATGCGCCGCCCAGACAGC
GATTTCCTGTATCGCGACGATCTGGCCGGCTGGCGGGTCGATGGCCGTCTGGCGCGATTGACCACCGCGAACTCGCGCGG
GGCAAAGCCGCGCCATGTCCAGGACGCGCTGCGGGCCGAGAGCGCTGAGCTGATCCGGCTGGTACACGAAGGCGCGCGCA
TCATGGTCTGCGGCGGGCGCGACATGGCCTCGGGCGTGGCCGAGGCGCTGACCGACATCCTCGCGCCGACGGGTCTTACC
CCGCTCGCGCTGAAAGCGGAGGGGCGCTATGCCGAAGATATCTACTGA

Upstream 100 bases:

>100_bases
GAACGACATCGCCGTGCCGTTGACGGCGGCAGGAGCGGGGCAGCCCGTGCCCGGCCGCCGCTATGTCGCCAGCCTCCGCT
ACGACATGTGAGATGCCGCC

Downstream 100 bases:

>100_bases
CCCGGGCCGCCACGCGCTGAACGGCCCGACCATGGGTACGCGCTGGTCGGCGCTTTTCCATGCCGATGCCGGGCTGGACC
CCGCCCCGGTGCAGGCGGCC

Product: oxidoreductase FAD/NAD(P)-binding subunit

Products: NA

Alternate protein names: SIR-FP [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MIRMLHRWPGLLALALVMVLAISGAALSVFPVAERFSTPQAEAGLTVADLAERIRTAYPGVEQIRRAPSGRITAFWFDDG
TPGAAVIDPATGKGIASADPNPVERWLTSLHRSLFLGDAGRIVMAAGAAAMLVLSVSGAMLVARRMGGWRRWFTPLRGPA
SSRIHTGIARVAVAGLILSSATALWMAASTFDLLPDGAAPPAFPTEVSGSTGVSLAAMEPLSVIPVTELRELSFPYPGDA
TDAFTLKTDRGTGYLDQGTGELLAWAELTMWERISETIYMLHTGQGAASLGLVLGVMALGVPAMGVSGLVLWFAGRRSRP
RLRGNHPAKGAGTILLVGSEGGSTWGFAATLQHALTTVGQHVHVAPMSGFDPARYPRAERFIVLSATWGEGQAPATAKGF
IERLAVLDSAPRAPLAVLGFGDRSFPAFCAFADEVARLAAAKGWAQLMPFDTVDRQSPQDFARWGRALGAALGIELALEH
VPTPPVTERLTLISRRDYGAEVQAPTTILRFALPEATFLQRVIARGFGRFQAGDLLGVLPEGADLPRFYSLASGRRDGFV
EIVVRKHPGGLCSGQLLALEPGQAIRAFVRHNPAFHAAAGRSPLILIGAGTGIGPLAGFIRGNSRKRPIHLAFGMRRPDS
DFLYRDDLAGWRVDGRLARLTTANSRGAKPRHVQDALRAESAELIRLVHEGARIMVCGGRDMASGVAEALTDILAPTGLT
PLALKAEGRYAEDIY

Sequences:

>Translated_735_residues
MIRMLHRWPGLLALALVMVLAISGAALSVFPVAERFSTPQAEAGLTVADLAERIRTAYPGVEQIRRAPSGRITAFWFDDG
TPGAAVIDPATGKGIASADPNPVERWLTSLHRSLFLGDAGRIVMAAGAAAMLVLSVSGAMLVARRMGGWRRWFTPLRGPA
SSRIHTGIARVAVAGLILSSATALWMAASTFDLLPDGAAPPAFPTEVSGSTGVSLAAMEPLSVIPVTELRELSFPYPGDA
TDAFTLKTDRGTGYLDQGTGELLAWAELTMWERISETIYMLHTGQGAASLGLVLGVMALGVPAMGVSGLVLWFAGRRSRP
RLRGNHPAKGAGTILLVGSEGGSTWGFAATLQHALTTVGQHVHVAPMSGFDPARYPRAERFIVLSATWGEGQAPATAKGF
IERLAVLDSAPRAPLAVLGFGDRSFPAFCAFADEVARLAAAKGWAQLMPFDTVDRQSPQDFARWGRALGAALGIELALEH
VPTPPVTERLTLISRRDYGAEVQAPTTILRFALPEATFLQRVIARGFGRFQAGDLLGVLPEGADLPRFYSLASGRRDGFV
EIVVRKHPGGLCSGQLLALEPGQAIRAFVRHNPAFHAAAGRSPLILIGAGTGIGPLAGFIRGNSRKRPIHLAFGMRRPDS
DFLYRDDLAGWRVDGRLARLTTANSRGAKPRHVQDALRAESAELIRLVHEGARIMVCGGRDMASGVAEALTDILAPTGLT
PLALKAEGRYAEDIY
>Mature_735_residues
MIRMLHRWPGLLALALVMVLAISGAALSVFPVAERFSTPQAEAGLTVADLAERIRTAYPGVEQIRRAPSGRITAFWFDDG
TPGAAVIDPATGKGIASADPNPVERWLTSLHRSLFLGDAGRIVMAAGAAAMLVLSVSGAMLVARRMGGWRRWFTPLRGPA
SSRIHTGIARVAVAGLILSSATALWMAASTFDLLPDGAAPPAFPTEVSGSTGVSLAAMEPLSVIPVTELRELSFPYPGDA
TDAFTLKTDRGTGYLDQGTGELLAWAELTMWERISETIYMLHTGQGAASLGLVLGVMALGVPAMGVSGLVLWFAGRRSRP
RLRGNHPAKGAGTILLVGSEGGSTWGFAATLQHALTTVGQHVHVAPMSGFDPARYPRAERFIVLSATWGEGQAPATAKGF
IERLAVLDSAPRAPLAVLGFGDRSFPAFCAFADEVARLAAAKGWAQLMPFDTVDRQSPQDFARWGRALGAALGIELALEH
VPTPPVTERLTLISRRDYGAEVQAPTTILRFALPEATFLQRVIARGFGRFQAGDLLGVLPEGADLPRFYSLASGRRDGFV
EIVVRKHPGGLCSGQLLALEPGQAIRAFVRHNPAFHAAAGRSPLILIGAGTGIGPLAGFIRGNSRKRPIHLAFGMRRPDS
DFLYRDDLAGWRVDGRLARLTTANSRGAKPRHVQDALRAESAELIRLVHEGARIMVCGGRDMASGVAEALTDILAPTGLT
PLALKAEGRYAEDIY

Specific function: Catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavo-protein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein compo

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=253, Percent_Identity=35.1778656126482, Blast_Score=104, Evalue=4e-22,
Organism=Homo sapiens, GI40254422, Length=187, Percent_Identity=36.3636363636364, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI127139033, Length=223, Percent_Identity=31.8385650224215, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI10835173, Length=187, Percent_Identity=31.5508021390374, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI169790956, Length=173, Percent_Identity=31.7919075144509, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI169790958, Length=173, Percent_Identity=31.7919075144509, Blast_Score=78, Evalue=4e-14,
Organism=Escherichia coli, GI1789123, Length=215, Percent_Identity=33.4883720930233, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554134, Length=203, Percent_Identity=30.5418719211823, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325305, Length=168, Percent_Identity=31.547619047619, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6321832, Length=160, Percent_Identity=34.375, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583543, Length=224, Percent_Identity=33.4821428571429, Blast_Score=79, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78706876, Length=224, Percent_Identity=33.4821428571429, Blast_Score=79, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78706872, Length=224, Percent_Identity=33.4821428571429, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 78110; Mature: 78110

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRMLHRWPGLLALALVMVLAISGAALSVFPVAERFSTPQAEAGLTVADLAERIRTAYPG
CCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHCCCCHHHHHHHHHHHCCC
VEQIRRAPSGRITAFWFDDGTPGAAVIDPATGKGIASADPNPVERWLTSLHRSLFLGDAG
HHHHHCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RIVMAAGAAAMLVLSVSGAMLVARRMGGWRRWFTPLRGPASSRIHTGIARVAVAGLILSS
CEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ATALWMAASTFDLLPDGAAPPAFPTEVSGSTGVSLAAMEPLSVIPVTELRELSFPYPGDA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCC
TDAFTLKTDRGTGYLDQGTGELLAWAELTMWERISETIYMLHTGQGAASLGLVLGVMALG
CCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHC
VPAMGVSGLVLWFAGRRSRPRLRGNHPAKGAGTILLVGSEGGSTWGFAATLQHALTTVGQ
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCC
HVHVAPMSGFDPARYPRAERFIVLSATWGEGQAPATAKGFIERLAVLDSAPRAPLAVLGF
EEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEC
GDRSFPAFCAFADEVARLAAAKGWAQLMPFDTVDRQSPQDFARWGRALGAALGIELALEH
CCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
VPTPPVTERLTLISRRDYGAEVQAPTTILRFALPEATFLQRVIARGFGRFQAGDLLGVLP
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECC
EGADLPRFYSLASGRRDGFVEIVVRKHPGGLCSGQLLALEPGQAIRAFVRHNPAFHAAAG
CCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCEEECCC
RSPLILIGAGTGIGPLAGFIRGNSRKRPIHLAFGMRRPDSDFLYRDDLAGWRVDGRLARL
CCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEECCCCCEEECCEEEEE
TTANSRGAKPRHVQDALRAESAELIRLVHEGARIMVCGGRDMASGVAEALTDILAPTGLT
EECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCC
PLALKAEGRYAEDIY
CEEEEECCCCCCCCC
>Mature Secondary Structure
MIRMLHRWPGLLALALVMVLAISGAALSVFPVAERFSTPQAEAGLTVADLAERIRTAYPG
CCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHCCCCHHHHHHHHHHHCCC
VEQIRRAPSGRITAFWFDDGTPGAAVIDPATGKGIASADPNPVERWLTSLHRSLFLGDAG
HHHHHCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
RIVMAAGAAAMLVLSVSGAMLVARRMGGWRRWFTPLRGPASSRIHTGIARVAVAGLILSS
CEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ATALWMAASTFDLLPDGAAPPAFPTEVSGSTGVSLAAMEPLSVIPVTELRELSFPYPGDA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCC
TDAFTLKTDRGTGYLDQGTGELLAWAELTMWERISETIYMLHTGQGAASLGLVLGVMALG
CCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHC
VPAMGVSGLVLWFAGRRSRPRLRGNHPAKGAGTILLVGSEGGSTWGFAATLQHALTTVGQ
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCC
HVHVAPMSGFDPARYPRAERFIVLSATWGEGQAPATAKGFIERLAVLDSAPRAPLAVLGF
EEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEC
GDRSFPAFCAFADEVARLAAAKGWAQLMPFDTVDRQSPQDFARWGRALGAALGIELALEH
CCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
VPTPPVTERLTLISRRDYGAEVQAPTTILRFALPEATFLQRVIARGFGRFQAGDLLGVLP
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECC
EGADLPRFYSLASGRRDGFVEIVVRKHPGGLCSGQLLALEPGQAIRAFVRHNPAFHAAAG
CCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCCEEECCC
RSPLILIGAGTGIGPLAGFIRGNSRKRPIHLAFGMRRPDSDFLYRDDLAGWRVDGRLARL
CCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEECCCCCEEECCEEEEE
TTANSRGAKPRHVQDALRAESAELIRLVHEGARIMVCGGRDMASGVAEALTDILAPTGLT
EECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCC
PLALKAEGRYAEDIY
CEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8695637 [H]