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The map label for this gene is apbE [H]

Identifier: 119386010

GI number: 119386010

Start: 456599

End: 457591

Strand: Direct

Name: apbE [H]

Synonym: Pden_3291

Alternate gene names: 119386010

Gene position: 456599-457591 (Clockwise)

Preceding gene: 119386009

Following gene: 119386011

Centisome position: 26.39

GC content: 70.39

Gene sequence:

>993_bases
ATGCCGAAGATATCTACTGACCCGGGCCGCCACGCGCTGAACGGCCCGACCATGGGTACGCGCTGGTCGGCGCTTTTCCA
TGCCGATGCCGGGCTGGACCCCGCCCCGGTGCAGGCGGCCTTGCAGGCGGCCGTGGCGGATGTCGACGCCCTGATGTCGA
CATGGAAACCCGACAGCGACCTGATGCGCCTGAACCGGACGCCCGAAGGTGTCCGGGTGATCGTTCCCGCCAGGTTGGCC
GAGGTGCTCGACCTCGGGCTGAGGATCGGGACCGCATCGGGCGGCGCGTTCGACATCGGCATGGGCGATGCGGCGCGCGC
GTGGGGTTTCGGGTCTGAGCCGGCCAATGCCGAGGCTATGCGCGCGGCGATGGCCACCGCGCGCCGGCCCGCGCAGGAAG
CGCTGGAGGTCGACCCTCTGGCCGGGCTGGCGCGAAAACACGCGCCGATCGCGCTCGATCTCAACGGCATCGCCAAGGGG
CATGGCGTCGACAGGCTGGCCAAGACGTTGCAGGGCTTCGGCATCACCGCGTTCCTTGTCGGCATCGACGGCGAAATGCG
TGCATCCGGGCTGCGACCCGATGGCATGCCGTGGACGGTCGCCGTCGAGATGCCCGATCCCGAACGCCGCGCGCCTCACT
CCATCCTGAAGCTCGCAGATGCCGCCGTCGCCACGTCGGGCGACTACCGCCACTATGTGACCCTTCGCGGCCGACGGCTG
TCGCACACGATGGACCCGACGACGGGCGGGCCGCTTCGCTCGTCGCCGGCCTCGGTCACCGTCGTCGCCCGCACCTGTGC
CGAAGCCGACGCCTGGGCGACCGCGCTGATGGTGCTGGGTCCGCAAGCCGGCGGCGAACTGGCTTCGCGTCTCGGCCTCC
ATGCGCTGTTCCTCATCCGCGAGGCAGGCGTCATCCGGACCCATGCCGTCGGGGCGCTGTTCGACAGCCCGATGGTATCG
GCGGCAGGCCGCGAGGGTCTGGTTCGTGCCTGA

Upstream 100 bases:

>100_bases
TGCGGCGGGCGCGACATGGCCTCGGGCGTGGCCGAGGCGCTGACCGACATCCTCGCGCCGACGGGTCTTACCCCGCTCGC
GCTGAAAGCGGAGGGGCGCT

Downstream 100 bases:

>100_bases
TGTCTGTCCATTCGTGCAATAGGTCTTTGCAGGTTTCGTCAATTTTCGTGCGAAAACGGACAGTCTATGTCTATCCCAAG
TCCGGCGGCGTCTGTCGATG

Product: ApbE family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MPKISTDPGRHALNGPTMGTRWSALFHADAGLDPAPVQAALQAAVADVDALMSTWKPDSDLMRLNRTPEGVRVIVPARLA
EVLDLGLRIGTASGGAFDIGMGDAARAWGFGSEPANAEAMRAAMATARRPAQEALEVDPLAGLARKHAPIALDLNGIAKG
HGVDRLAKTLQGFGITAFLVGIDGEMRASGLRPDGMPWTVAVEMPDPERRAPHSILKLADAAVATSGDYRHYVTLRGRRL
SHTMDPTTGGPLRSSPASVTVVARTCAEADAWATALMVLGPQAGGELASRLGLHALFLIREAGVIRTHAVGALFDSPMVS
AAGREGLVRA

Sequences:

>Translated_330_residues
MPKISTDPGRHALNGPTMGTRWSALFHADAGLDPAPVQAALQAAVADVDALMSTWKPDSDLMRLNRTPEGVRVIVPARLA
EVLDLGLRIGTASGGAFDIGMGDAARAWGFGSEPANAEAMRAAMATARRPAQEALEVDPLAGLARKHAPIALDLNGIAKG
HGVDRLAKTLQGFGITAFLVGIDGEMRASGLRPDGMPWTVAVEMPDPERRAPHSILKLADAAVATSGDYRHYVTLRGRRL
SHTMDPTTGGPLRSSPASVTVVARTCAEADAWATALMVLGPQAGGELASRLGLHALFLIREAGVIRTHAVGALFDSPMVS
AAGREGLVRA
>Mature_329_residues
PKISTDPGRHALNGPTMGTRWSALFHADAGLDPAPVQAALQAAVADVDALMSTWKPDSDLMRLNRTPEGVRVIVPARLAE
VLDLGLRIGTASGGAFDIGMGDAARAWGFGSEPANAEAMRAAMATARRPAQEALEVDPLAGLARKHAPIALDLNGIAKGH
GVDRLAKTLQGFGITAFLVGIDGEMRASGLRPDGMPWTVAVEMPDPERRAPHSILKLADAAVATSGDYRHYVTLRGRRLS
HTMDPTTGGPLRSSPASVTVVARTCAEADAWATALMVLGPQAGGELASRLGLHALFLIREAGVIRTHAVGALFDSPMVSA
AGREGLVRA

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=313, Percent_Identity=33.5463258785943, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 34456; Mature: 34325

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKISTDPGRHALNGPTMGTRWSALFHADAGLDPAPVQAALQAAVADVDALMSTWKPDSD
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
LMRLNRTPEGVRVIVPARLAEVLDLGLRIGTASGGAFDIGMGDAARAWGFGSEPANAEAM
HHHHCCCCCCEEEEEHHHHHHHHHHHHEEECCCCCEEEECCCCHHHHCCCCCCCCCHHHH
RAAMATARRPAQEALEVDPLAGLARKHAPIALDLNGIAKGHGVDRLAKTLQGFGITAFLV
HHHHHHHCCCHHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEEE
GIDGEMRASGLRPDGMPWTVAVEMPDPERRAPHSILKLADAAVATSGDYRHYVTLRGRRL
ECCCCHHHCCCCCCCCCEEEEEECCCCHHHCHHHHHHHHHHHHHCCCCCEEEEEEECCEE
SHTMDPTTGGPLRSSPASVTVVARTCAEADAWATALMVLGPQAGGELASRLGLHALFLIR
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHH
EAGVIRTHAVGALFDSPMVSAAGREGLVRA
HCCCHHHHHHHHHHCCCHHHHCCCCCCCCC
>Mature Secondary Structure 
PKISTDPGRHALNGPTMGTRWSALFHADAGLDPAPVQAALQAAVADVDALMSTWKPDSD
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
LMRLNRTPEGVRVIVPARLAEVLDLGLRIGTASGGAFDIGMGDAARAWGFGSEPANAEAM
HHHHCCCCCCEEEEEHHHHHHHHHHHHEEECCCCCEEEECCCCHHHHCCCCCCCCCHHHH
RAAMATARRPAQEALEVDPLAGLARKHAPIALDLNGIAKGHGVDRLAKTLQGFGITAFLV
HHHHHHHCCCHHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEEE
GIDGEMRASGLRPDGMPWTVAVEMPDPERRAPHSILKLADAAVATSGDYRHYVTLRGRRL
ECCCCHHHCCCCCCCCCEEEEEECCCCHHHCHHHHHHHHHHHHHCCCCCEEEEEEECCEE
SHTMDPTTGGPLRSSPASVTVVARTCAEADAWATALMVLGPQAGGELASRLGLHALFLIR
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHH
EAGVIRTHAVGALFDSPMVSAAGREGLVRA
HCCCHHHHHHHHHHCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]