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The map label for this gene is ilvB [H]

Identifier: 119385994

GI number: 119385994

Start: 441327

End: 443006

Strand: Direct

Name: ilvB [H]

Synonym: Pden_3275

Alternate gene names: 119385994

Gene position: 441327-443006 (Clockwise)

Preceding gene: 119385993

Following gene: 119385995

Centisome position: 25.51

GC content: 66.55

Gene sequence:

>1680_bases
ATGAAGGGGCCGCGCACTGCCGCCCGCATCCTCGTCGACCAGCTGTTGCTGCAAGGTGTGGACCGCGTCTTCTGCGTGCC
GGGCGAAAGCTTCCTCGCCGTTCTCGACGCGCTGCATGACTGCCCCCAGATCGAATTGGTGACCTGTCGGCACGAGGGCG
GGGCCGCCATGATGGCCGAGGCCTATGGCAAGCTGACCGGCCGGCCGGGCGTCGTCTTCTGCTCGCGCGGCCCCGGGGCA
ACCAATGCCTCCTCGGGGCTTCATGTCGCCTTTCAGGACAGCACGCCGATGATCCTGTTCATCGGCCAGAACCCGCGTGA
CGTGATGACCCGCGAGGGGTTTCAGGAGATCGACTACCGCTGCACTTTCGGCACGCAGGCCAAGGCTGTGCTGCAGCTCG
AGGACGCCGCACGGACCGCGGAATTCGTCACCCGCGCCTTTCAGCTGGCCATGTCCGGCCGTCCCGGTCCCGTGGTCATT
CCCCTGCCGGAGGACGTGCTGACCGAAATCGCCGAATGCGCCGATGGCCGCCCTGCGGCGCGCTTTGCGAATGAGCCCTC
GCCGGAGGCCGTGTCGGCGGTGCGGGCATTGCTGACCGAGGCGCGCCGTCCGGTCGTGATGCTGGGCGGCTCCGGCTGGA
CCGGCGAGGCGGTCGCCGCCTTCCGTCGCTTTGCCGAGGACAGCGGGCTGCCCGTCACCGCCTCGTTCCGGCGGCAGGAC
CTCCTCGACAATGACAGCAGCAGCTATATCGGGGAACTCGGCCTCGGCCCCAATCCCAGGCTTGTGCAGATGGTGGCGGA
GGCGGATCTGATCCTCGTCATCGGGGCGCGGTTGGGCGAGATCGGAACCAATGGCTACACGATCATGGACATTCCCTGTC
CCCGGCAGCGGTTGATCCATGTCTATCCCGGTCCTGATGAGATCGGCCATGTCTATCGACCCGAACTGGGCATCGTCAGC
GATCCGGCAAGCTTTGCCCTGGCGCTCGCCGCGGGCGGTGCTGTCCGCGGTGATTGGGACGACTGGCGCAAGGCCGCACG
CGCGGATTTCGAGGCATGGACCGCCCCTCGGACCAGCCCCGGCGCGATCCAGATGTGCGAGATCATGGCCTGGCTTGATG
AGAGCCTGCCGGAGGACACGATCTACACCAACGGCGCAGGGAATTTTACCGTCTGGCTGCACCGCTTTCACCGCTATCGC
CGCTTTGGCACGCAGGTCGCGCCGATCTGCGGCTCGATGGGCTACGGCATTCCGGCAGCCTTGGCCGCCAAGTTCGCCCG
GCCAGAAGCCTCTGTTATTTGCATCGCAGGGGACGGCGATTTCCAGATGACCTGCACCGAGTTGGCGACGGCGGCGGCGC
ATGGGCTGAAAATCCGTATCCTGATCCTGAACAACGGGATGCTCGGCACGATCCGCATGCATCAGGAGCGGCATTATCCC
GCCCGGGTCTCGGGAACCGATCTCGCGCGCGGAAATCCCGATTTCACCGCATTGGCGCGGTCCTATGGCGCCCATGCCGA
AAGGGTCGAGCGGACCACGGATTTCGCCGCAGCCTTCGCGCGCGCCGACACCCATGACGGGCCCGCGGTCATCGAAATCA
TGATCGATCCCGAGGCTCTGACGCCCGGCGCCTCGCTCAGCCAGATCAGGCAGGCGGCGCTGCAGACGCTGCCGGGCTAA

Upstream 100 bases:

>100_bases
TGGAGCTTTCGCCCGAGGACCGGCACGCGATCTTCGAAGGCAATGTGCGCCGCGTCTATCCGCGCCTCGACGCGCGGCTG
AAGGCGATGGGAAAAGCGGC

Downstream 100 bases:

>100_bases
ATCCAGGGACCATATTGGAAGTCAACCTGCGACCATTGGAATTGAGCTTGCCCTTATTCTCGGAGATGCCGGGCAGGCCT
AACCTGAAAGACATGAAGTC

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MKGPRTAARILVDQLLLQGVDRVFCVPGESFLAVLDALHDCPQIELVTCRHEGGAAMMAEAYGKLTGRPGVVFCSRGPGA
TNASSGLHVAFQDSTPMILFIGQNPRDVMTREGFQEIDYRCTFGTQAKAVLQLEDAARTAEFVTRAFQLAMSGRPGPVVI
PLPEDVLTEIAECADGRPAARFANEPSPEAVSAVRALLTEARRPVVMLGGSGWTGEAVAAFRRFAEDSGLPVTASFRRQD
LLDNDSSSYIGELGLGPNPRLVQMVAEADLILVIGARLGEIGTNGYTIMDIPCPRQRLIHVYPGPDEIGHVYRPELGIVS
DPASFALALAAGGAVRGDWDDWRKAARADFEAWTAPRTSPGAIQMCEIMAWLDESLPEDTIYTNGAGNFTVWLHRFHRYR
RFGTQVAPICGSMGYGIPAALAAKFARPEASVICIAGDGDFQMTCTELATAAAHGLKIRILILNNGMLGTIRMHQERHYP
ARVSGTDLARGNPDFTALARSYGAHAERVERTTDFAAAFARADTHDGPAVIEIMIDPEALTPGASLSQIRQAALQTLPG

Sequences:

>Translated_559_residues
MKGPRTAARILVDQLLLQGVDRVFCVPGESFLAVLDALHDCPQIELVTCRHEGGAAMMAEAYGKLTGRPGVVFCSRGPGA
TNASSGLHVAFQDSTPMILFIGQNPRDVMTREGFQEIDYRCTFGTQAKAVLQLEDAARTAEFVTRAFQLAMSGRPGPVVI
PLPEDVLTEIAECADGRPAARFANEPSPEAVSAVRALLTEARRPVVMLGGSGWTGEAVAAFRRFAEDSGLPVTASFRRQD
LLDNDSSSYIGELGLGPNPRLVQMVAEADLILVIGARLGEIGTNGYTIMDIPCPRQRLIHVYPGPDEIGHVYRPELGIVS
DPASFALALAAGGAVRGDWDDWRKAARADFEAWTAPRTSPGAIQMCEIMAWLDESLPEDTIYTNGAGNFTVWLHRFHRYR
RFGTQVAPICGSMGYGIPAALAAKFARPEASVICIAGDGDFQMTCTELATAAAHGLKIRILILNNGMLGTIRMHQERHYP
ARVSGTDLARGNPDFTALARSYGAHAERVERTTDFAAAFARADTHDGPAVIEIMIDPEALTPGASLSQIRQAALQTLPG
>Mature_559_residues
MKGPRTAARILVDQLLLQGVDRVFCVPGESFLAVLDALHDCPQIELVTCRHEGGAAMMAEAYGKLTGRPGVVFCSRGPGA
TNASSGLHVAFQDSTPMILFIGQNPRDVMTREGFQEIDYRCTFGTQAKAVLQLEDAARTAEFVTRAFQLAMSGRPGPVVI
PLPEDVLTEIAECADGRPAARFANEPSPEAVSAVRALLTEARRPVVMLGGSGWTGEAVAAFRRFAEDSGLPVTASFRRQD
LLDNDSSSYIGELGLGPNPRLVQMVAEADLILVIGARLGEIGTNGYTIMDIPCPRQRLIHVYPGPDEIGHVYRPELGIVS
DPASFALALAAGGAVRGDWDDWRKAARADFEAWTAPRTSPGAIQMCEIMAWLDESLPEDTIYTNGAGNFTVWLHRFHRYR
RFGTQVAPICGSMGYGIPAALAAKFARPEASVICIAGDGDFQMTCTELATAAAHGLKIRILILNNGMLGTIRMHQERHYP
ARVSGTDLARGNPDFTALARSYGAHAERVERTTDFAAAFARADTHDGPAVIEIMIDPEALTPGASLSQIRQAALQTLPG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=575, Percent_Identity=24.1739130434783, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI93004078, Length=572, Percent_Identity=22.3776223776224, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=29.1814946619217, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI87081685, Length=534, Percent_Identity=29.4007490636704, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI1786717, Length=550, Percent_Identity=28, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787096, Length=575, Percent_Identity=24.1739130434783, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1788716, Length=587, Percent_Identity=22.9982964224872, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17531299, Length=572, Percent_Identity=25.1748251748252, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=572, Percent_Identity=25.1748251748252, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17542570, Length=591, Percent_Identity=23.0118443316413, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=558, Percent_Identity=28.494623655914, Blast_Score=229, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6320816, Length=289, Percent_Identity=23.8754325259516, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19922626, Length=585, Percent_Identity=25.1282051282051, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60113; Mature: 60113

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGPRTAARILVDQLLLQGVDRVFCVPGESFLAVLDALHDCPQIELVTCRHEGGAAMMAE
CCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
AYGKLTGRPGVVFCSRGPGATNASSGLHVAFQDSTPMILFIGQNPRDVMTREGFQEIDYR
HHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHCCHHHCCEE
CTFGTQAKAVLQLEDAARTAEFVTRAFQLAMSGRPGPVVIPLPEDVLTEIAECADGRPAA
EECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCHH
RFANEPSPEAVSAVRALLTEARRPVVMLGGSGWTGEAVAAFRRFAEDSGLPVTASFRRQD
HCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHH
LLDNDSSSYIGELGLGPNPRLVQMVAEADLILVIGARLGEIGTNGYTIMDIPCPRQRLIH
HCCCCCCCCEEECCCCCCHHHHHHHHCCCEEEEEECHHCCCCCCCEEEEECCCCHHHEEE
VYPGPDEIGHVYRPELGIVSDPASFALALAAGGAVRGDWDDWRKAARADFEAWTAPRTSP
EECCCCCCCCCCCCCCCEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHCCCCCCC
GAIQMCEIMAWLDESLPEDTIYTNGAGNFTVWLHRFHRYRRFGTQVAPICGSMGYGIPAA
CHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHH
LAAKFARPEASVICIAGDGDFQMTCTELATAAAHGLKIRILILNNGMLGTIRMHQERHYP
HHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCC
ARVSGTDLARGNPDFTALARSYGAHAERVERTTDFAAAFARADTHDGPAVIEIMIDPEAL
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
TPGASLSQIRQAALQTLPG
CCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGPRTAARILVDQLLLQGVDRVFCVPGESFLAVLDALHDCPQIELVTCRHEGGAAMMAE
CCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
AYGKLTGRPGVVFCSRGPGATNASSGLHVAFQDSTPMILFIGQNPRDVMTREGFQEIDYR
HHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCHHHHHHCCHHHCCEE
CTFGTQAKAVLQLEDAARTAEFVTRAFQLAMSGRPGPVVIPLPEDVLTEIAECADGRPAA
EECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCHH
RFANEPSPEAVSAVRALLTEARRPVVMLGGSGWTGEAVAAFRRFAEDSGLPVTASFRRQD
HCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHH
LLDNDSSSYIGELGLGPNPRLVQMVAEADLILVIGARLGEIGTNGYTIMDIPCPRQRLIH
HCCCCCCCCEEECCCCCCHHHHHHHHCCCEEEEEECHHCCCCCCCEEEEECCCCHHHEEE
VYPGPDEIGHVYRPELGIVSDPASFALALAAGGAVRGDWDDWRKAARADFEAWTAPRTSP
EECCCCCCCCCCCCCCCEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHCCCCCCC
GAIQMCEIMAWLDESLPEDTIYTNGAGNFTVWLHRFHRYRRFGTQVAPICGSMGYGIPAA
CHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHH
LAAKFARPEASVICIAGDGDFQMTCTELATAAAHGLKIRILILNNGMLGTIRMHQERHYP
HHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCC
ARVSGTDLARGNPDFTALARSYGAHAERVERTTDFAAAFARADTHDGPAVIEIMIDPEAL
CCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
TPGASLSQIRQAALQTLPG
CCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]